Long COVID in People With Multiple Sclerosis and Related Disorders: A Multicenter Cross-Sectional Study.

Background: Managing long COVID in people with multiple sclerosis and related disorders (pwMSRD) is complex due to overlapping symptoms. To address evidence gaps, we evaluated long COVID susceptibility in pwMSRD versus controls and its associations with multi-domain function and disability.

Methods: In this multicenter cross-sectional study, participants completed a survey covering 71 post-infection symptoms, distinguishing new-onset from worsening symptoms.

Blood pressure in the first 6 hours for older adults with stroke after endovascular therapy: a pooled analysis of the DEVT and RESCUE BT randomized clinical trials.

Optimal systolic blood pressure (SBP) targets after endovascular therapy (EVT) for stroke in older adults (≥ 65 years) remain undefined. This study assessed age-stratified associations between early post-EVT SBP (first 6 h) and outcomes. Post hoc analysis of two trials.

Exercise interventions in cancer-related cognitive impairment: an umbrella review.

Purpose: The aim of this umbrella review is to assess the effectiveness of exercise interventions in preventing and managing cancer-related cognitive impairment among cancer survivors, providing an evidence-based foundation for clinical practice.

Methods: The umbrella review was pre-registered on PROSPERO. It included systematic reviews that assessed any exercise interventions aimed at improving cognition in cancer patients.

MGRL-DDI: Multiview Graph Representation Learning for Accurate Drug-Drug Interaction Prediction.

Drug-drug interactions (DDIs) present a significant challenge in clinical practice, as they may lead to adverse reactions, diminished therapeutic efficacy, and serious risks to patient safety. However, most existing methods depend on single-view representations of drug molecules or substructures, which limits their capacity to capture the diverse and complex nature of drug properties. To overcome this limitation, we propose MGRL-DDI, a multiview graph representation learning framework that comprehensively models drug structures from three complementary perspectives: Three-dimensional (3D) molecular graphs, motif graphs, and molecular graphs.

Identification and exploration of novel biomarkers and potential therapeutic agents for the progression of sepsis to septic ARDS.

Patients with sepsis complicated by acute respiratory distress syndrome (ARDS) face a significantly increased risk of in-hospital death. This study aimed to identify sepsis-associated genes involved in ARDS pathogenesis and discover candidate biomarkers for its diagnosis. Gene expression profiling data from the Gene Expression Omnibus database were analyzed to identify key septic ARDS genes using differential expression analysis and weighted gene co-expression network analysis.

Broad-spectrum antitumor analysis of the telomerase activity inhibitor TPCH derived from the human constitutively expressed protein LPTS/PinX1.

Background: The human liver-related putative tumor suppressor LPTS/PinX1 is a gene encoding a telomerase inhibitory protein. Overexpression of LPTS/PinX1 protein can inhibit the growth of multiple telomerase-positive cancer cell lines. LPTS/PinX1 has therapeutic potential for cancer.

Short-course-based TNT with or without PD-1 inhibitor for pMMR locally advanced rectal cancer: Phase 2 results of a randomized trial (STELLAR II).

Background: The therapeutic efficacy and mode of combining immunotherapy with neoadjuvant chemoradiotherapy in proficient mismatch repair (pMMR)/microsatellite stable (MSS) locally advanced rectal cancer (LARC) remain uncertain.

Methods: In this multicenter, randomized, seamless phase 2/3 trial (ClinicalTrials.gov: NCT05484024), eligible participants were randomly assigned (1:1) to receive short-course radiotherapy (SCRT) (5 Gy × 5), followed by 4 cycles of capecitabine and oxaliplatin or 6 cycles of leucovorin, oxaliplatin, and fluorouracil, with (iTNT group) or without (total neoadjuvant therapy [TNT] group) 4 cycles of sintilimab.

Visible light-driven activation of peroxymonosulfate by tubular O, S co-doped g-CN for efficient aflatoxin B degradation in edible oils.

Aflatoxin B (AFB) contamination in edible oils poses severe food safety threats, with traditional photocatalysis hindered by insufficient reactive oxygen species (ROS) precursors in lipid matrices. This study integrates tubular O, S co-doped g-CN (OSTCN) photocatalysis into edible oil water-washing, in the simulated dual-phase systems, OSTCN activates peroxymonosulfate (PMS) under visible light to generate ROS, achieving complete degradation of oil-phase migrated parent AFB within 120 min while maintaining oil quality within acceptable thresholds. Combined evidence reveals that the tubular structure enhances light harvesting, while O, S co-doping creates electron donor-acceptor sites that strengthen PMS adsorption and electron transfer, reducing activation barriers and conferring 6.

Age-Related Trends in Total Joint Arthroplasty in China: A Nationwide Study.

Background: Changes in age-related trends in joint arthroplasty have a significant impact on surgical prognosis and resource allocation. Though these trends have been documented in several high-income countries, the rapid aging process and relatively backward development of joint arthroplasty may shape a different pattern in China. Therefore, we aimed to determine the age-related trends in total knee arthroplasty (TKA) and total hip arthroplasty (THA) in China.

MAFLD outperforms NAFLD in identifying metabolic dysfunction in U.S. adolescents: a NHANES 2017-2020 analysis.

Background: The newly proposed metabolic-associated fatty liver disease (MAFLD) definition better reflects the metabolic pathogenesis shared with non-alcoholic fatty liver disease (NAFLD), though with incomplete diagnostic overlap; this study systematically compares their prevalence and risk profiles in U.S. adolescents to inform optimal clinical management strategies.

Draft genome sequence of SZCHProba001, isolated from feces of a 6-year-old boy.

is a prevalent human gut bacterium. Here, we report the draft genome of SZCHProba001 isolated from a 6-year-old boy's feces. The 5.

Computationally resolved neuroprogenitor cell biomarkers associate with human disorders.

Adult hippocampal neurogenesis, the process of generating new neurons, relies on a rare population of neural stem and progenitor cells (NPCs) within the dentate gyrus complex microenvironment. Discovering the specific genes that define these cells is vital yet challenging due to overlapping expression patterns, limiting detection of rare cell populations using traditional approaches. By employing the computational digital sorting algorithm (DSA) that deconvolves complex gene expression data based on pattern recognition, we identified 129 genes enriched in murine NPCs.

Enhanced pollutant removal in moving bed biofilm reactor under high-salinity condition via specialized quorum sensing bacteria.

The study aims to accelerate biofilm formation and operational performance of moving bed biofilm reactor (MBBR) in a high salt environment by adding specific quorum sensing (QS) strain (Vibrio sp. LV-Q1). Results showed that the addition of QS strain significantly promoted the pollutant removal process of the bioreactor.

Dapk2 dysfunction leads to Mic60 lactylation and mitochondrial metabolic reprogramming, promoting lung cancer EGFR-TKI resistance and metastasis.

The epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) resistance is often linked to tumor metastasis, making control of metastasis crucial. Here, we identified a critical signaling hub responsible for cancer metastasis and resistance development: mitochondrial cristae remodeling and metabolic reprogramming, using an anoikis-resistant cell model and a mouse tail vein metastasis model. EGFR-TKI-resistant cells exhibited stronger anoikis resistance (AR) and mitochondrial metabolism compared with sensitive cells, making them more prone to metastasis.

The dependence of CO cerebrovascular reactivity (CVR) on caffeine.

Cerebrovascular reactivity (CVR) represents an important marker of brain vascular health, particularly in the context of small and large vessel diseases. However, an undesired feature of this measure is that there exist large variations in CVR values across individuals, which is mainly attributed to physiological factors. Here, we test the hypothesis that caffeine, a widely consumed neurostimulant, has a significant effect on CVR measured with MRI.

Research on the predictive efficacy of MRI-based endplate bone quality and vertebral body bone quality scores for adjacent segment degeneration after lumbar spinal fusion.

Objective: This study aims to evaluate the predictive significance of scores relating to endplate bone quality (EBQ) and vertebral body bone quality (VBQ) as assessed by MRI for adjacent segment degeneration (ASD) following posterior lumbar interbody fusion (PLIF).

Methods: In this retrospective analysis, we examined 236 patients with degenerative conditions of the lumbar spine who had undergone PLIF between 2018 and 2022. Participants were categorized into groups based on radiographic ASD (RASD), symptomatic ASD (SASD), and no ASD (NASD).

Tumor-infiltrating B cells produce tumor-specific antibodies and may contribute to suppressing tumor in head and neck squamous cell carcinoma.

The involvement of T cells, particularly cytotoxic T cells, in tumor immunity has been reported, but the roles of B cells and antibodies in tumor immunity remain unclear. Previous studies have primarily focused on circulating B cells in the blood, with limited investigation of tumor-infiltrating B cells (TIL-Bs) and their secreted antibodies in head and neck squamous cell carcinoma (HNSCC) tissue. This study aimed to clarify TIL-Bs' role in HNSCC through comprehensive analyses including single-cell RNA sequencing of 68 cases, B-cell receptor repertoire analysis, multiplex immunofluorescence staining, and spatial transcriptomics analysis of resected HNSCC specimens.

Correction: Analyzing cold hardiness (Based on DTA) of one-year-old branches of peaches.

[This corrects the article DOI: 10.1371/journal.pone.

Improvement of anti-swelling property and anticorrosion performance of epoxy coatings by the introduction of nanocrystalline cellulose composite.

In this work, the effect of nanocrystalline cellulose (NCC) as a nanofiller on the corrosion protection of epoxy resin coatings was examined. To solve problems like poor solvent swelling resistance and micropore formation during the solidification and shrinkage of epoxy resin coatings, NCC was modified with a silane coupling agent. Alkyl chains and active amine groups were grafted onto its surface, improving the dispersibility of NCC in organic solvents and its bonding with epoxy resin.

EGCG-releasing nanofibrous scaffold enhances wound healing via γδ T cells modulation.

This study investigates the role of γδ T cells in wound healing facilitated by an EGCG (Epigallocatechin gallate)-releasing nanofibrous scaffold with aligned topography (APEMS). Incorporating core-shell microcapsules for sustained EGCG release over 11 days, the biocompatible scaffold enhances γδ T cell activity to promote advanced wound repair. In a splinted wound excisional model in 6-8 weeks-old male C57BL/6J mice, APEMS significantly improved re-epithelialization and wound closure compared to a control scaffold (APS).

Recombinant human collagen microneedle patches loaded with PRP for diabetic wound treatment.

Chronic nonhealing wounds represent significant complications of diabetes, bearing a substantial burden and posing risks of disability or mortality. In diabetic wounds, continuous tissue fluid exudation, inflammatory cell migration, fibrosis, and bacterial biofilm formation create a "barrier", which decreases the treating efficacy of therapeutics. To address these limitations, a recombinant human collagen type III microneedle patch (rhCol III-PRP) loaded with platelet-rich plasma (PRP) was developed, in which methacrylated rhCol III (rhCol III-MA) loaded with PRP was utilized to form needle tips, while rhCol III-MA formed the base part of the patch.

Screening of host proteins interacting with the African swine fever virus outer membrane protein CD2v.

Background: The African swine fever virus (ASFV) is a highly pathogenic double-stranded DNA virus that poses a significant threat to the global swine industry. Although the CD2v protein encoded by ASFV is a key factor in viral immune evasion and pathogenicity, the mechanism underlying its interaction with host proteins remains unclear.

Methods: The aim of this study was to identify a range of potential host proteins that interact with the CD2v protein using the membrane yeast two-hybrid technology.

Biomechanical effects of asymmetric backpack shoulder straps on the unilateral flatfoot: a finite element analysis.

This study investigated the biomechanical effects of asymmetric backpack shoulder strap configurations on individuals with unilateral flatfoot (UF) using finite element analysis (FEA). A male subject with UF was recruited, and different shoulder strap settings were simulated to assess their impact on plantar fascia and Achilles tendon stress and strain, as well as on changes in foot arch morphology. The results showed that using equal-length straps on both shoulders resulted in lower stress and strain levels in the plantar fascia and Achilles tendon during walking.

An Intrinsic-hoc Framework for Heterogeneous Cellular Senescence Elucidation Using Deep Graph Representation Learning and Experimental Validation.

Characterizing senescent cells and identifying corresponding senescence-associated genes within complex tissues is critical for our understanding of aging and age-related diseases. We present DeepSAS, an intrinsic-hoc framework for elucidating the heterogeneity encoded in senescent cells and their associated genes from single-cell RNA-seq data using deep graph representation learning. Applied to both healthy eye cell atlas and in-house idiopathic pulmonary fibrosis datasets with Xenium spatial transcriptomics validation, DeepSAS reveals robust and biologically grounded senotypes and demonstrates superior benchmarking performance compared with existing methods.