Identification and exploration of novel biomarkers and potential therapeutic agents for the progression of sepsis to septic ARDS.

Patients with sepsis complicated by acute respiratory distress syndrome (ARDS) face a significantly increased risk of in-hospital death. This study aimed to identify sepsis-associated genes involved in ARDS pathogenesis and discover candidate biomarkers for its diagnosis. Gene expression profiling data from the Gene Expression Omnibus database were analyzed to identify key septic ARDS genes using differential expression analysis and weighted gene co-expression network analysis.

Risk factors in systemic lupus erythematosus-associated Sjögren's syndrome: multicenter case-control studies and systematic review.

Objective: The study identifies risk factors for SLE-associated Sjögren's syndrome (SLE-SS), based on multiple cohorts and meta-analysis.

Methods: Three cohorts (Luzhou cohort, Enshi cohort, and Chengdu cohort) were initially conducted, and clinical, laboratory characteristics from patients with SLE-only and SLE-SS patients were collected and we discussed risk factors related to SLE-SS. Subsequently, a meta-analysis discussed risk factors related to SLE-SS risk from published articles and our studies.

Linear covariation between germline and somatic mutation rates across ciliates and mammals.

Somatic mutations have received increased attention due to their roles in disease (e.g., cancer) and possibly aging.

Efficacy and safety of heart rate control with esmolol on the incidence and duration of organ failure in predicted severe acute pancreatitis: protocol of a multicenter, open-label, randomized controlled trial.

Introduction: Overactivation of the sympathetic nerve system can lead to a sustained increase in heart rate, which may impair blood perfusion and organ function. Previous studies have demonstrated that the use of -blockers like esmolol can reduce heart rate, thereby improving clinical outcomes in patients with septic shock. For acute pancreatitis (AP), which shares a similar inflammatory pathophysiology with sepsis, previous experimental and observational studies showed significant sympathetic excitation during the acute phase, and the use of -blockers might be clinically beneficial.

Development and validation of a prognostic nomogram incorporating automated collateral score and computed tomography perfusion parameters for patients with acute ischemic stroke undergoing endovascular thrombectomy.

Background: Prognostic evaluation of patients with acute ischemic stroke (AIS) after endovascular thrombectomy (EVT) remains challenging. Traditional computed tomography angiography (CTA) and computed tomography perfusion (CTP) have limitations, whereas multiphase CTA (mCTA) allows for more accurate collateral circulation assessment. However, the predictive utility of automated collateral scoring remains underexplored.

Interfacial Anchoring and Dual-Phase Separation Tame Liquid Metal into a NIR-Responsive Conductive Adhesive for Remote Electronics Welding and Eco-Recycling.

We present a near-infrared (NIR)-weldable and recyclable conductive adhesive through solvent-free integration of a liquid metal (LM) with polythioctic acid (pTA). The strong thiol/carboxyl-mediated binding enables 70 wt % LM loading within the pTA matrix while preventing leakage. Crystallization of incompletely polymerized TA monomers generates microphase domains that enhance the bulk cohesion of pTA/LM, achieving an adhesion strength exceeding 1.

Investigation of the molecular mechanism of Xiaoluo Wan in thyroid-associated ophthalmopathy: network analysis and in vivo study.

Background: Thyroid-associated ophthalmopathy (TAO) is a common complication of hyperthyroidism that can significantly impair quality of life. This study investigated the effects and mechanisms of Xiaoluo Wan (XLW), a traditional Chinese herbal prescription, in treating TAO.

Methods: The protective effects of XLW on the extraocular muscles were first examined in hyperthyroid rats.

Fufang Shuanghua oral liquid alleviates influenza A virus-infected acute lung injury by restoring antiviral immunity partially via TNF-mediated IFN/NLRP3-inflammasome signaling.

Ethnopharmacological Relevance: As a traditional Chinese medicine (TCM)-originated antiviral prescription, Fufang Shuanghua oral liquid (FS) is extensively used for influenza virus therapy with a satisfying therapeutic efficacy in clinic.

Aim Of The Study: The bioactive compounds (BACs) and the molecular mechanisms of FS against influenza A virus (IAV)-infected acute lung injury were investigated.

Materials And Methods: IAV-infected acute lung injury mouse model was established infected by IAV (H1N1) strain A/PR/8/34 to evaluate the pharmacological effects of FS.

miR-363-5p- and IL-34-mediated modulation of pacemaker channel, HCN4, on iPSC-CM: Translation into the human sinus node microenvironment.

Background: The human sinus node (SN) contains cardiac fibroblasts and resident macrophages, with microRNAs (miRNAs) and interleukins as regulators of SN function. However, the mechanisms by which they influence heart rate remain unclear.

Objective: This study aimed to investigate the SN microenvironment, encompassing miRNAs, interleukins, macrophages, and fibroblasts and modulating induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and hence beating rate.

Targeting Ferroptosis With Natural Products to Treat Diabetes and Its Complications: Opportunities and Challenges.

Diabetes mellitus (DM) is a common chronic metabolic disorder, and the recommended pharmacotherapies in DM guidelines can effectively alleviate symptoms of DM, but some drugs may have certain side effects. In contrast, natural products and their derivatives have shown promising therapeutic effects on DM with fewer side effects. Additionally, ferroptosis, as a form of cell death, is involved in the normal physiological and pathological regulation of the body.

Single-Cell RNA Sequencing Identifies MMP11 Cancer-Associated Fibroblasts as Drivers of Angiogenesis and Bladder Cancer Progression.

Cancer-associated fibroblasts (CAFs) play a crucial role in tumor progression, with heterogeneity influencing therapeutic response and prognosis, highlighting their potential as viable targets for treatment. In this study, a novel CAF subgroup, MMP11 mCAF is identified, through single-cell RNA sequencing, which accumulates progressively during bladder cancer progression and is significantly associated with poor prognosis. This cell population regulates the migration of tip endothelial cell clusters (ESM1tEC) via the WNT5A-MCAM signaling axis, and modulates the expression of key transcription factors, SOX18, NFIC, and HOXB9.

Caveolin-1 negatively regulates the calcitonin receptor-like receptor and neuroinflammation in a female mouse model of migraine.

Background: Caveolin-1 (CAV1), a scaffolding protein critical for caveolae formation, regulates G-protein-coupled receptor (GPCR) signaling via caveolae-mediated endocytosis. The calcitonin receptor-like receptor (CLR), a GPCR and core subunit of the calcitonin gene-related peptide (CGRP) receptor, is a therapeutic target for migraine. However, the role of CAV1 in CLR regulation and migraine remains unclear.

Prediction of a novel synthetic peptide vaccine against tuberculosis and validation of its immunogenicity.

Background: Tuberculosis (TB) is an infectious disease transmitted through the respiratory system that affects people worldwide. Bacillus Calmette-Guérin (BCG), the only approved TB vaccine, has been shown to have highly variable protective efficacy in different populations and is ineffective at protecting adults. Therefore, the development of more effective TB vaccines is vital.

Kaempferol Mitigates -Induced Acute Lung Inflammation Through Suppressing GSK3β/JNK/c-Jun Signaling Pathway and NF-κB Activation.

, one of the common bacterial pathogens causing nosocomial pneumonia, is characterized as highly pathogenic and multidrug-resistant. Kaempferol (KP), a natural flavonoid, has been shown to exhibit effectiveness in treating infection-induced lung injury. We applied network pharmacology to explore the underlying mechanisms of KP in treating pneumonia and further validated them through a mouse model of acute bacterial lung infection and an in vitro macrophage infection model.

Functionalized Cerium Oxide Nanoparticles Enhance Penetration into Melanoma Spheroids In Vivo through Angiogenesis.

Angiogenesis is a crucial step in tumor progression, including melanoma, making anti-angiogenic strategies a widely explored treatment approach. However, both innate and acquired resistance to these therapies suggest that this approach may need re-evaluation. Nanoparticles have gained attention for their potential to enhance drug delivery and retention within tumors via the bloodstream.

The interplay between endothelial glycocalyx maturity and both the toxicity and intracellular uptake of charged nanoparticles.

Nanoparticles are widely studied for delivering treatments to target tissues, but few have reached clinical use. Most nanoparticles encounter blood vessels on their way to target tissues. The inner surface of these vessels is lined with endothelial cells covered by a glycocalyx, an extracellular matrix rich in anionic glycans.

[Current situation investigation and analysis of influencing factors on the long-term quality of life of cured and discharged patients with severe acute pancreatitis].

Objective: To investigate the current status of long-term quality of life in patients with severe acute pancreatitis (SAP) who have been cured and discharged, and to analyze the influencing factors affecting long-term quality of life in SAP cured patients after discharge.

Methods: A retrospective collection was conducted. Patients who were received standardized treatment before being cured and discharged from the hospital admitted to the first department of critical care medcine of the Second Affiliated Hospital of Anhui Medical University from January 2017 to December 2023 were enrolled.

A Risk Prediction Model for the Development of Rheumatoid Arthritis, Sjögren's Syndrome, Systemic Sclerosis in Patients With Systemic Lupus Erythematosus: Multicenter Approaches.

Aim: The aim of this study was to construct a predictive model to evaluate the risk of overlapping syndrome (OS), including the combination of systemic sclerosis, rheumatoid arthritis, and Sjögren's syndrome in patients with systemic lupus erythematosus (SLE) from multicenters.

Methods: This study included SLE patients in the development cohort (n = 4714) and the external validation cohort (n = 2271). SLE patients in the development cohort were randomly divided into the training cohort and the internal validation cohort at a 7:3 ratio.

Searching for potential Culicoides vectors of four orbiviruses in Yunnan Province, China.

Background: Some species of Culicoides (Diptera, Ceratopogonidae) are major vectors for arboviruses, and Yunnan Province is a key area for arbovirus prevalence in China. Therefore, this study attempts to search for potential Culicoides vectors for the common orbiviruses bluetongue virus (BTV), epizootic hemorrhagic disease virus (EHDV), Palyam virus (PALV) and Tibet orbivirus (TIBOV) in Yunnan Province, China.

Methods: Culicoides specimens were collected from 16 counties in Yunnan Province, China, using UV traps and tested for BTV, EHDV, PALV and TIBOV through one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

Identification and Functional Analysis of PANoptosis-Associated Genes in the Progression From Sepsis to ARDS.

Background: Sepsis and acute respiratory distress syndrome (ARDS) are common inflammatory conditions in intensive care, with ARDS significantly increasing mortality in septic patients. PANoptosis, a newly discovered form of programmed cell death involving multiple cell death pathways, plays a critical role in inflammatory diseases. This study aims to elucidate the PANoptosis-related genes (PRGs) and their involvement in the progression of sepsis to ARDS.

Impact of Mild Field Drought on the Aroma Profile and Metabolic Pathways of Fresh Tea () Leaves Using HS-GC-IMS and HS-SPME-GC-MS.

Aroma plays a pivotal role in defining tea quality and distinctiveness, and tea producers have often observed that specific drought conditions are closely associated with the formation and accumulation of characteristic aroma compounds in tea leaves. However, there is still limited understanding of the differential strategies employed by various tea cultivars in response to drought stress for the accumulation of key volatile aroma compounds in fresh tea leaves, as well as the associated metabolic pathways involved in aroma formation. In this study, two widely cultivated tea cultivars in China, Fuding Dabai (FD) and Wuniuzao (WNZ), were examined to assess the impact of mild field drought stress on the composition and accumulation of key volatile aroma compounds in fresh leaves using headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace solid phase micro-extraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) technologies.

The evolution of Traditional Chinese Medicine as recombinant inventions.

It has been widely recognized that technologies evolve with recombinant inventions. However, it remains unknown whether technologies developed using different approaches would exhibit different features during evolution. In particular, would technologies developed mainly based on accumulated experience in practices-formulas of traditional Chinese medicine (TCM) are typical examples of such technologies-have similar evolution features found in modern technologies? This study applied network science to explore the evolution of TCM from the perspective of recombinant inventions based on 59,063 TCM formulas documented over the last two thousand years, with each formula being a combination of components that are mostly herbs.

Effectiveness of a Triple Antiarrhythmic Drug Strategy for Arrhythmia Recurrence after Persistent Atrial Fibrillation Ablation.

Background And Objective: Treating recurrent atrial arrhythmias after persistent atrial fibrillation (PeAF) ablation is often challenging. This single-center, prospective study aimed to observe the effectiveness of different combinations of oral antiarrhythmic drugs (AADs) in reverting to sinus rhythm (SR) in patients with recurrent atrial arrhythmias after PeAF ablation.

Methods: Forty-five patients who experienced recurrent atrial arrhythmias after PeAF ablation were included.