Dietary Exposure to Imidacloprid at Environmental Doses Induces Renal Injury in Mice via P-AMPK Inhibition and Subsequent Purine Metabolism Dysregulation.

Imidacloprid (IMI), a neonicotinoid insecticide extensively applied in agriculture, is among the most frequently detected pesticides in food. However, the long-term impact of chronic dietary exposure to environmentally relevant IMI doses on kidney health has not been fully elucidated, particularly regarding its nephrotoxic mechanisms. To address this gap, chronic exposure to IMI was administered to mice through supplementation of their feed with environmentally relevant doses (approximately 100 and 1000 μg/kg/day) for 24 weeks to investigate its nephrotoxicity and underlying mechanisms.

Enhanced chemiluminescence driven by Fe-MIL-101(NH) host-guest recognition for the detection of glutamic acid.

Enhancing luminol-based chemiluminescence (CL) emission through functionalized materials is essential for developing highly sensitive method in rapid analysis. Herein, a Fe-MIL-101(NH) enhanced CL method was developed for the selective determination of glutamic acid (Glu) in serum. By utilizing Fe-MIL-101(NH) as an effective catalyst for the first time, the CL intensity of the luminol-HO system was amplified, and this system exhibited a selective response to Glu.

Butterfly-Shaped Folding Synthons for Designing Superselective and Ultrapermeable Artificial Water Channels.

Water transport across biological membranes is essential for life, facilitated by water channel proteins like aquaporins (AQPs). Drawing inspiration from these natural systems, artificial water channels (AWCs) have emerged as transformative tools for advancing industrial and environmental applications. Herein, we report the design and comprehensive characterization of a groundbreaking class of AWCs, derived from unprecedented butterfly-shaped aromatic folding synthons, carefully engineered to emulate the functional attributes of natural AQPs.

Alkoxy functionalized covalent organic frameworks for efficient oil/water separation.

The massive discharge of oily wastewater and the frequent occurrence of oil spills have posed serious threat to human. Hence, it is important to develop functional materials and related methods to achieve efficient oil/water separation. Herein, a new superhydrophobic COF (named SCOF) was designed and successfully synthesized with functional alkoxy side chains at room temperature for the first time and was then applied to oil/water separation.

Precision fabrication of polymer nanostructures on recyclable DNA template.

The fabrication of precisely patterned polymers at the nanoscale is of critical importance. We have previously succeeded in creating various nanopatterned polymers with nanoscale resolution through the use of in situ atom transfer radical polymerization (ATRP) techniques on deoxyribonucleic acid (DNA) origami. However, separating nanopatterned polymers from the origami template without damaging the origami presents a significant challenge, thereby increasing costs and limiting the development of applications involving nanopatterned polymers.

Chronic Exposure to Environmentally Relevant Doses of Triphenyl Phosphate Induces Anxiety- and Depression-Like Behaviors in Mice via the Gut-Brain Axis.

Triphenyl phosphate (TPHP) is an emerging endocrine-disrupting chemical that has been widely used as a flame retardant. Although the neurotoxicity of TPHP to aquatic animals had been highlighted in previous studies, its effects on mammals, particularly at environmentally relevant doses (ERDs), have remained largely unknown. In this study, a 28-week mouse model was established to investigate the potential neurotoxicity induced by ERDs of TPHP (0.

Constructing Immunomodulator Biosynthesis Factory in Grafting-From DNA Hydrogel for Heart Valve Regeneration.

The development of heart valve prostheses with regenerative capabilities offers significant potential to overcome the limitations of existing commercial artificial valves in clinical practice. Immune modulation plays a crucial role in heart valve regeneration by reversing the coagulation and inflammatory microenvironment, thereby facilitating recellularization. In this study, a biosynthesis factory is constructed on decellularized heart valves (DHVs) to continuously convert the abundant heme in the blood into immunomodulators, supporting long-term immune modulation and tissue regeneration.

Capsule-Type Single-Crystal Covalent Organic Frameworks as an Advanced Chromatographic Stationary Phase for Efficient Separation of Isomers.

Single-crystal covalent organic frameworks (SCOFs) with long-range ordered crystal structures have emerged as potential separation media for high-performance liquid chromatography (HPLC). Herein, capsule-type SCOFs (C-SCOFs) were synthesized at room temperature and directly utilized as a stationary phase for HPLC. Owing to its regular morphology, excellent monodispersity, outstanding chemical stability, organized pore structure, and pronounced hydrophobicity, C-SCOFs packed column could not only efficiently separate hydrophobic molecules with outstanding column efficiency but also achieved baseline separation of 15 isomers with similar physicochemical properties, including iodoanisole isomers, iodotoluene isomers, and iodobenzotrifluoride isomers.

Donor-acceptor covalent organic framework nanofilm-based laser desorption/ionization mass spectrometry for rapid and sensitive determination of creatinine in human serum.

Creatinine (Cre), a metabolite generated by muscles and kidneys, holds significant importance in clinical screening and detection of kidney disease. However, the existing clinical detection of Cre, such as the Jaffe reaction-based colorimetric method, requires complex sample pretreatment and is subject to interference in biological samples. Herein, a surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) method based on a donor-acceptor covalent organic framework (D-A COF) nanofilm as a substrate was proposed for Cre determination in human serum.

Determination of perfluoroalkyl carboxylic acids in mice serum and tissue by dispersive solid-phase extraction based on fluorine-functionalized covalent organic frameworks coupled with UPLC-MS/MS.

Perfluoroalkyl carboxylic acids (PFCAs) are a class of persistent organic pollutants (POPs), which posed various hazards to organisms, including reproductive, developmental, neurological, and immunological toxicity. Consequently, developing an analytical method aimed at achieving highly sensitive detection of trace amounts of PFCAs in complex samples is clearly of great significance. Herein, the Fluorine-functionalized covalent organic framework (F-COF) was synthesized at ambient temperature, which has the advantages of high specific surface area (1297.

Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for the Determination of Benzophenone Derivatives in Personal Care Products.

Rational: Benzophenone derivatives, commonly used as UV filters in personal care products (PCPs), are widely prevalent and raise concerns due to their endocrine-disrupting effects. Sensitive and efficient analytical methods are in demand for their detection. In this study, we developed a TAPB-DMTP-covalent organic framework (COF) nanofilm-assisted laser desorption ionization mass spectrometry (LDI-MS) method for the quantitative analysis of 2,4-dihydroxybenzophenone (BP-1) in PCPs.

Construction of hierarchically porous covalent organic frameworks with enhanced enzyme accessibility for sensitive dopamine detection.

Immobilization of fragile enzymes is vital to expanding its application in the extracellular environment. Covalent organic frameworks (COFs), as a class of emerging porous materials, are promising platforms for enzyme immobilization owing to their high porosity and tunable structure. However, the interior pores of COFs often fail to play their roles because of inaccessibility, resulting in decreased performance of immobilized enzymes.

Facile synthesis of hierarchically flower-like hollow covalent organic frameworks for enrichment and metabolic analysis of benzophenone derivatives in mouse serum.

Benzophenone derivatives (BPs), as synthetic chemicals widely used in personal care products, have drawn increasing attention due to their potential health risks. However, monitoring BPs in biological samples remains challenging due to their complex matrices and the deficiency in sensitivity and selectivity in current methods. Herein, a method combining hierarchically flower-like hollow covalent organic frameworks (HFH-COFs) with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for the enrichment and detection of BPs in serum samples.

Metal covalent organic frameworks-based laccase-like nanozyme for oxidative degradation and identification of phenolic pollutants.

Phenolic compounds are a kind of persistent organic pollutants (POPs), which are always a threat to human and environment due to their strong toxicity and low biodegradability. Therefore, developing a reliable method to simultaneously detect phenolic pollutants is of great importance to environmental safety and human health. Herein, we combined the advantages of metal organic frameworks (MOFs) and covalent organic frameworks (COFs) to prepare two cyclic trinuclear unit-based metal covalent organic framework (MCOFs, denoted as Cu-TDH COF and Cu-BDU COF) with large specific surface area, good stability and excellent laccase-like activity.

Gold-Modified Covalent Organic Frameworks-Assisted Laser Desorption/Ionization Mass Spectrometry for Analysis of Metabolites Induced by Triclosan Exposure.

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) holds great promise for the rapid and sensitive detection of biomolecules, but its precise detection of small molecule metabolites is hindered by severe background interference from the organic matrix in the low molecular weight range. To address this issue, nanomaterials have commonly been utilized as substrates in LDI-MS. Among them, covalent organic frameworks (COFs), known for their unique optical absorption and structural properties, have garnered significant attention.

Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for Rapid Screening of Parabens in Personal Care Products.

Rational: People are widely exposed to parabens in their daily life, but parabens are endocrine disrupting chemicals that pose a threat to human health. Therefore, establishing a rapid screening method to enhance monitoring of parabens is necessary. Herein, a covalent organic framework (COF) nanofilm-assisted laser desorption ionization mass spectrometry (LDI-MS) method was established to screen parabens in personal care products (PCPs).

1,4-Dioxo-1,2,3,4-tetrahydrophthalazine-6-carboxylic acid as a novel MALDI matrix for enhanced analysis of metabolites induced by imidacloprid exposure.

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has become a robust tool for analyzing a variety of biomacromolecules. However, the strong background interference produced by conventional organic matrices hinders the detection of small molecule analytes, which restricts the widespread application of MALDI-MS in metabolomics studies. Consequently, developing new organic matrices is urgently needed to overcome these issues.

Spatial metabolomics reveal metabolic alternations in the injured mice kidneys induced by triclocarban treatment.

Triclocarban (TCC) is a common antimicrobial agent that has been widely used in medical care. Given the close association between TCC treatment and metabolic disorders, we assessed whether long-term treatment to TCC at a human-relevant concentration could induce nephrotoxicity by disrupting the metabolic levels in a mouse model. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was applied to investigate the alterations in the spatial distributions and abundances of TCC, endogenous and exogenous metabolites in the kidney after TCC treatment.

Covalent organic framework-based solid phase microextraction coupled with electrospray ionization mass spectrometry for the quantitative assessment of abnormal bile acids by triclosan exposure in mice.

Bile acids, a representative diagnostic indicator of liver function, are used to visualize the extent of liver injury. Numerous studies have shown that triclosan (TCS) exposure leads to abnormal bile acid metabolism. As a result, there is a requirement to develop a fast and smart means to quantitatively monitor abnormal bile acids from exposure to triclosan in bio-sample.

Bioinspired Iron Porphyrin Covalent Organic Frameworks-Based Nanozymes Sensor Array: Machine Learning-Assisted Identification and Detection of Thiols.

Given the crucial role of thiols in maintaining normal physiological functions, it is essential to establish a high-throughput and sensitive analytical method to identify and quantify various thiols accurately. Inspired by the iron porphyrin active center of natural horseradish peroxidase (HRP), we designed and synthesized two iron porphyrin covalent organic frameworks (Fe-COF-H and Fe-COF-OH) with notable peroxidase-like (POD) activity, capable of catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) into oxidized TMB with three distinct absorption peaks. Based on these, a six-channel nanozyme colorimetric sensor array was constructed, which could map the specific fingerprints of various thiols.

Long-term exposure to triclocarban induces splenic injuries in mice: Insights from spatial metabolomics and lipidomics.

Triclocarban (TCC) is a widely used antimicrobial agent and known endocrine-disrupting chemical found in various products. While its potential toxicities on endocrine-related organs have been highlighted in previous studies, the effects of TCC on non-endocrine organs, particularly the spleen, remain largely unknown. Here, we employed a novel approach combining long-term TCC exposure in a mouse model with spatial metabolomics and lipidomics to investigate the effects of TCC on the spleen.

Advances in covalent organic frameworks for sample preparation.

Sample preparation is crucial in analytical chemistry, impacting result accuracy, sensitivity, and reliability. Solid-phase separation media, especially adsorbents, are vital for preparing of liquid and gas samples, commonly analyzed by most analytical instruments. With the advancements in materials science, covalent organic frameworks (COFs) constructed through strong covalent bonds, have been increasingly employed in sample preparation in recent years.

[A comprehensive undergraduate experiment: preparation and chromatographic evaluation of a core-shell stationary phase based on a covalent organic framework].

Continuously promoting new curriculum standards is a key aim of the Ministry of Education of China. With this in mind, this paper introduces a comprehensive teaching experiment for undergraduate instrumental analysis courses that aims to improve students' material-preparation and instrumental-analysis skills through practice. Herein, a covalent organic framework-based core-shell stationary phase material (SiO@COF) is prepared via a one-pot method and characterized in detail, after which its chromatographic properties are evaluated by high performance liquid chromatography (HPLC).

Associations of mothers' decisional conflicts and satisfaction with governmental health promotion materials with their daughters' HPV vaccination uptake in China: A cross-sectional survey.

Background: China started to implement the HPV vaccination program for females in 2016. This study investigated associations between mothers' decisional conflicts, satisfaction with governmental health promotion materials, and their daughters' HPV vaccination uptake.

Methods: A cross-sectional online survey was conducted between July and October 2023 among mothers of girls aged 9-17 years in Shenzhen, China.