Assessment of serum Ninj1 as a potential biomarker for predicting severity in patients with COVID-19.

Infection with SARS-CoV-2 elevates the expression of cytokines, resulting in a cytokine storm that serves as the primary factor for severe illness and mortality; however, effective markers for predicting disease severity and preventing are lacking. Thus, we investigated the association between serum levels of nerve injury-induced protein 1 (Ninj1), a mediator of plasma membrane rupture, and the extent of lung damage in COVID-19 patients was examined to anticipate the severity of SARS-CoV-2 infection. This study included 62 healthy participants and 264 patients with COVID-19.

The Effects of HFOV-VG vs HFOV on Bronchopulmonary Dysplasia and Neurobehavioral Development in Preterm Infants with Perinatal Acute Respiratory Distress Syndrome.

Objective: This study aimed to explore whether high-frequency oscillatory ventilation with volume-guarantee (HFOV-VG) strategy could reduce the incidence of bronchopulmonary dysplasia (BPD) and improve poor neurological prognosis in premature infants with perinatal acute respiratory distress syndrome (NARDS) compared with high-frequency oscillatory ventilation (HFOV) alone.

Methods: This retrospective single-center study conducted in the neonatal intensive care unit (NICU) from January 2016 and December 2023. One hundred and seventy-two premature infants (32 weeks ≤ gestational age < 37 weeks) with NARDS were enrolled.

Electrically tunable terahertz filter based on single split-ring resonators integrated with graphene.

We present a compact, electrically reconfigurable terahertz (THz) filter based on a single split-ring resonator (SRR) bridged by gate-tuned graphene ribbons. By varying the graphene Fermi level from 0 to 1.5 eV, we continuously modulate the SRR's gap capacitance and realize a reversible blue-shift of the LC resonance from 1.

Clinical relevance of transperineal ultrasound compared with anorectal manometry for the evaluation of female patients with obstructive defecation syndrome.

Background And Aims: The utility of transperineal ultrasound (TPUS) in combination with anorectal manometry (ARM) in the evaluation of women with obstructive defecation syndrome (ODS) remains unclear. We investigated the combined performance of TPUS and ARM in the evaluation of female patients with ODS, and determined the clinical utility of both modalities in the evaluation of female patients with ODS.

Methods: Female patients with ODS were evaluated by TPUS and ARM, and binary logistic regression models were exploited to evaluate the clinical association of abnormal TPUS variables with abnormal ARM variables.

LC-MS Determination of Trichloroethylene Glutathione Conjugation Metabolites in a Parkinson's Disease Mouse Model.

Rational: Investigating TCE glutathione (GSH) conjugation metabolites is essential, as these reactive intermediates play a central role in TCE bioactivation and are implicated in organ-specific toxicities, including nephrotoxicity, hepatotoxicity, and neurotoxicity. Characterizing and quantifying these metabolites enhances our understanding of TCE metabolism, supports biomarker discovery, and helps elucidate mechanisms of TCE-induced toxicity.

Methods: A robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for the detection and quantification of three major GSH-related TCE metabolites: S-(1,2-dichlorovinyl)-glutathione (DCVG), S-(1,2-dichlorovinyl)-L-cysteine (DCVC), and N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine (NAcDCVC).

tRNA modification profiling reveals epitranscriptome regulatory networks in Pseudomonas aeruginosa.

Transfer RNA (tRNA) modifications have emerged as critical post-transcriptional regulators of gene expression affecting diverse biological and disease processes. While there is extensive knowledge about the enzymes installing the dozens of post-transcriptional tRNA modifications-the tRNA epitranscriptome-very little is known about how metabolic, signaling, and other networks integrate to regulate tRNA modification levels. Here, we took a comprehensive first step at understanding epitranscriptome regulatory networks by developing a high-throughput tRNA isolation and mass spectrometry-based modification profiling platform and applying it to a Pseudomonas aeruginosa transposon insertion mutant library comprising 5746 strains.

Levels and predictors of PTSS in young children aged 3-12 years at the hardest-hit area of China during early COVID-19 outbreak.

Background: Public crisis has a profound impact on mental health, particularly among young children, since that they are often encountered with disrupted routine, restricted movement, school closure, caregiver illness or distress, greater likelihood of aversive or neglecting parenting etc. However, the mental distress of young children aged below 12 years-old has been less attended. Based on the Risk Factor Model, this study aims to observe the levels and predictors of posttraumatic stress symptoms (PTSS) in children aged 3-12 years at the hardest-hit area of China during the early stage of the Coronavirus Disease 2019 (COVID-19) outbreak.

Red cabbage exosome-like nanoparticles inhibited LPS-induced inflammation and oxidative stress in RAW264.7 cells.

Unlabelled: Excessive inflammation and oxidative stress can accelerate inflammatory disease. Plant exosome-like nanoparticles demonstrate therapeutic capacity for anti-inflammatory and antioxidant effects. Here, the effects of red cabbage exosome-like nanoparticles (RELNs) on inflammation and oxidative stress triggered by LPS in macrophages were investigated.

Development and validation of a nomogram model for predicting the occurrence of necrotizing enterocolitis in premature infants with late-onset sepsis.

Background: Necrotizing enterocolitis (NEC), a devastating gastrointestinal disease in preterm infants, is strongly linked to sepsis, and 34-57% of NEC cases develop post-sepsis. However, the risk factors for sepsis-associated NEC are still unclear. Therefore, this study aimed to identify predictive factors associated with the occurrence of NEC in premature infants with late-onset sepsis (LOS) and establish a nomogram for early NEC prediction.

Impact of Molecular Tumor Board on Clinical Outcomes in Patients with Refractory Solid Tumor: a Real-World Study.

Introduction: Providing precise oncologic treatment for patients with refractory solid tumor is still an unmet need in clinical practice. This study aimed to assess whether treatments recommended by a molecular tumor board (MTB) can improve clinical outcomes in patients with refractory solid tumors.

Methods: We screened all patients with refractory solid tumor during the period from 2017 to 2022 at the authors' center.

How emotional labor leads to turnover intention: the mediating role of job burnout and the moderating role of social support among rural physical education teachers in China.

Objective: To examine the mediating role of job burnout in the relationship between emotional labor strategies and turnover intention among rural physical education (PE) teachers in China, and to explore the moderating effect of social support in this mechanism.

Methods: A cross-sectional survey was conducted among 1,761 rural PE teachers using validated self-report scales. Structural equation modeling (SEM) via AMOS 26.

Effect of COVID-19 inactivated vaccine on anti-Müllerian hormone in Chinese women: a retrospective cohort study.

Introduction: This study aimed to assess the impact of inactivated COVID-19 vaccine on Anti-Müllerian hormone (AMH) levels in Chinese women.

Methods: A retrospective analysis was conducted on women aged 18-45 who had undergone two AMH tests between March 2020 and September 2021. Participants were grouped based on vaccine doses (two- and three-dose), time intervals since vaccination, and manufacturers.

Evaluating rapid antimicrobial susceptibility testing directly from positive blood cultures for Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in Chinese hospitals.

Introduction: We sought to assess whether rapid antimicrobial susceptibility testing (AST) directly from positive blood culture bottles for Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa can be used in Chinese hospitals.

Methods: Rapid AST was performed on each positive blood culture bottle. Categorical rapid AST results at 8 to 10 and 16 to 18 hours were compared with those from the VITEK 2 system (bioMérieux).

Helicobacter Pylori Infection as an Independent Risk Factor for Kidney Stone Formation in China: A Cross-Sectional Study.

Introduction: This cross-sectional study aimed to explore the relationship between Helicobacter pylori (Hp) infection and kidney stone formation in China, given the high incidence of Hp infection and its potential to cause damage to multiple systems.

Methods: Conducted at the Department of Health Management of Wuhan Union Hospital, the study included 48,294 hp-negative and 28,455 hp-positive individuals. Hp infection was detected using the 13C urea breath test (13C-UBT), and kidney stones were identified via urinary Doppler ultrasonography.

Cytokine profiling in COVID-19 patients by using ion mobility-mass spectrometry-based parallel reaction monitoring.

Cytokine therapy, a non-antigen-specific strategy, has led to several FDA-approved drugs. Given the role of dysregulated cytokine expression in diseases such as COVID-19, accurate quantification is critical in both clinical and research settings. While antibody-based assays offer high sensitivity, their reliance on specific antibodies limits multiplexing and increases analytical complexity.

Comparative safety and efficacy of manual therapy interventions for cervicogenic headache: a systematic review and network meta-analysis.

Objective: To evaluate and compare the safety and efficacy of spinal manipulation, mobilization, and massage for the management of cervicogenic headache (CGH) using meta-analytic techniques.

Methods: Comprehensive searches were conducted in Cochrane, Embase, PubMed, and ClinicalTrials.gov to identify studies investigating the effects of manipulation, mobilization, and massage on pain, disability, and physical function in patients with CGH.

Anlotinib plus toripalimab as a first-line treatment in patients with advanced gastric cancer and performance status 2: the phase II APICAL-GC trial.

Evidence-guided regimens for advanced gastric cancer (AGC) in patients with performance status 2 (PS 2) are limited. Here, we proposed a structured therapeutic framework termed "performance status-matched strategy", and further conducted the APICAL-GC trial (NCT04278222). This open-label, single-arm phase II study evaluated the efficacy and safety of anlotinib combined with toripalimab among 24 treatment-naïve AGC patients with PS 2.

[Safety evaluation of new drugs of traditional Chinese medicine based on human use experience].

Because of the unclear active substances, metabolic pathways, and targets of new drugs of traditional Chinese medicine(TCM), non-clinical safety evaluation often fails to accurately locate the target organs and tissue exposed to medicinal toxicity. The human use experience(HUE) contains important safety information of TCM, while the clinical safety data in the past HUE are few and have not been effectively applied. Standardized prospective HUE studies should be carried out to collect the clinical safety data, in which appropriate physical and chemical indicators(including blood, urine, and stool routine), liver biochemical indicators, kidney biochemical indicators, and cardiovascular biochemical indicators should be selected for safety evaluation, and the detection time point and sample size should be rationally designed.

Functional and Numerical Responses of (Hemiptera: Miridae) to (Hemiptera: Delphacidae).

The predatory function and numerical responses of natural enemy insects are critical for evaluating their biological control potential, particularly in understanding pest suppression mechanisms and ecological adaptability. Here, we examined the predation capacity of Stål (Hemiptera: Miridae) on eggs under laboratory conditions (24 ± 1 °C, 12:12 h light:dark photoperiod, 75% humidity). Functional response experiments were conducted at different prey densities (3, 4, 10, 20, and 30 eggs/day), and life table parameters were established to evaluate the effects of prey density on the development, reproduction, and population dynamics of .

Conductive cellulose nanofibers tailored poly(propylene carbonate) island structure and synergistic reinforcement-toughening mechanism of high-performance poly(lactic acid) composites.

Biodegradable poly(lactic acid) (PLA) based composites have broad application prospects in replacing common polyester-based composites, but poor toughness and low thermal stability of PLA composites is still unsatisfactory for practical applications. Herein, a new preparation method of PLA composites by doping rigid conductive cellulose nanofibers (CNFene) and tough poly(propylene carbonate) (PPC) to tailor island structure and synergistic reinforcement-toughening mechanism is presented. Moreover, the composite films and paper-based coated beverage straws prepared from these composites has excellent thermal stability, high-strength, and high-toughness.

Manual Therapy for Cervical Radiculopathy: Effects on Neck Disability and Pain - A Systematic Review and Network Meta-Analysis.

Objective: To evaluate the application effects of different manual therapy approaches in the treatment of cervical radiculopathy using a network meta-analysis.

Methods: Prospective randomized controlled trials on manual therapy for cervical radiculopathy published in PubMed, the Cochrane Library, and Embase databases were retrieved. The neck disability index and visual analogue scale for neck pain were collected and subjected to network meta-analysis.

How self-efficacy shapes professional identity: the mediating role of meaning in life and self-esteem in pre-service physical education teachers.

Background: Professional identity is a crucial psychological construct that shapes pre-service physical education (PE) teachers' academic engagement, persistence, and future career commitment. However, limited research has examined the psychological mechanisms that foster the development of professional identity in this population. This study explores how self-efficacy contributes to the formation of professional identity among PE pre-service teachers, with a particular focus on the mediating roles of meaning in life and self-esteem.

Exploring the mitigating potential of anthocyanin Malvidin in a mouse model of bleomycin-induced pulmonary fibrosis by inhibiting NLRP3 inflammasome activation and oxidative stress.

Background: Malvidin (MV), an essential anthocyanin, has antioxidant and anti-inflammatory effects that may help treat pulmonary fibrosis (PF), a progressive and occasionally fatal condition characterized by severe lung scarring, oxidative stress, and inflammation.

Objective: This study aims to evaluate the therapeutic potential of MV in PF by assessing its effects on inflammation, oxidative stress, and fibrotic markers through in vitro and in vivo models.

Methods And Materials: The compound was evaluated by molecular docking.

In-Tip Nanoreactors for Simultaneous Proteolysis and Enrichment of Phosphorylated Peptides.

Protein phosphorylation introduces negative charges on the hydroxyl groups of serine, threonine, and tyrosine residues, reducing the ionization efficiency of phosphorylated peptides. The low abundance of phosphorylated peptides often diminishes their detection using mass spectrometry. To enhance the identification of the low-abundance peptides, an enrichment step was often used, which complicated the high-throughput analysis of phosphorylated proteomes.

Tea Extracellular Vesicle-Derived MicroRNAs Contribute to Alleviate Intestinal Inflammation by Reprogramming Macrophages.

The clinical use of conventional medications for inflammatory bowel disease (IBD) is often limited by significant side effects. The extracellular vesicles derived from plant-based diets have shown promise in mitigating disease. Here, we discovered that natural extracellular vesicles from tea (TEVs) can achieve an appropriate transition from proinflammatory (M1) to anti-inflammatory (M2) macrophages and inhibit inflammation response both in vitro and in vivo.