Hypoxia Inducible Factor-1α Specific Probe for Tumor Hypoxia Positron Emission Tomography Imaging.

A peptide-based, hypoxia-inducible factor-1 α (HIF-1α) specific PET tracer for tumor hypoxia imaging is reported. It was prepared with a rapid AlF labeling method with high stability. AlF-CLLFVY specifically binds to HIF-1α with high affinity and shows higher uptake in cells under hypoxia.

Hierarchical Structures of Protein-Fiber-Infused Aminoglycan Hydrogels to Promote Myocardial Repair.

Myocardial infarction (MI) is followed by irreversible damage to the myocardium, which eventually evolves into ventricular remodeling and heart failure. An imbalanced inflammatory response after MI can exacerbate myocardial injury. Current strategies to modulate inflammation and thereby improve myocardial tissue repair are limited.

Inhalable Nanovaccine Based on Bioengineered Bacteria-Derived Membrane Vesicles Against Lung Metastasis.

Lung metastases pose a challenge in cancer treatment due to the lung's vascular network and immunosuppressive microenvironment. Conventional subcutaneous vaccines typically fail to elicit localized immune responses at metastatic sites. To address this, an inhalable nanovaccine, BMVax (bacterial membrane-based vaccine), is developed using bacterial membrane vesicles from engineered E.

Ionic strength effects on rheology, microstructure, and properties of diacylglycerol-based high internal phase Pickering emulsion-myofibrillar protein gels.

This study examined the effects of various ionic strengths (0, 0.1, 0.3, and 0.

Visualizing energy transfer between redox-active colloids.

Redox-active colloids (RACs) represent a novel class of energy carriers that exchange electrical energy upon contact. Understanding contact-mediated electron transfer dynamics in RACs offers insights into physical contact events in colloidal suspensions and enables quantification of electrical energy transport in nonconjugated polymers. Redox-based electron transport was directly observed in monolayers of micron-sized RACs containing ethyl-viologen side groups via fluorescence microscopy through an unexpected nonlinear electrofluorochromism that is quantitatively coupled to the redox state of the colloid.

Functional connectivity signatures of olfactory networks linked to cognitive risk: A functional MRI study in subjective cognitive decline at risk for Alzheimer's disease.

BackgroundIndividuals with subjective cognitive decline (SCD) are at high risk of preclinical Alzheimer's disease (AD). While olfactory dysfunction is evident in AD and mild cognitive impairment (MCI), its presence and neural mechanism in SCD remain unclear.ObjectiveThis study examined functional connectivity (FC) alterations across olfactory networks and their mediating role between olfactory and cognitive functions in SCD and MCI.

[Role of traditional Chinese medicine concepts in the prevention of childhood caries].

The high prevalence of childhood caries poses a considerable threat to children's overall health. Traditional Chinese medicine (TCM) concepts offers a distinctive and valuable perspective on caries prevention. This study explores TCM's conceptualization of dental caries in relation to the physiological characteristics of children and proposes targeted preventive strategies, including holistic care, dietary regulation, and lifestyle modification.

Long-term outcomes of venetoclax and ibrutinib in Japanese patients with relapsed/refractory mantle cell lymphoma.

Background: Patients with relapsed/refractory (R/R) mantle cell lymphoma (MCL) face a poor prognosis in the absence of effective treatment options. Ibrutinib plus venetoclax demonstrated high response rates and a tolerable safety profile in the primary analysis of the Phase 2, M20-075 study (NCT04477486) in Japanese patients with R/R MCL. We report updated efficacy and safety from this study with longer follow-up.

Toward High-Voltage Cathodes for Zinc-Ion Batteries: Discovery Pipeline and Material Design Rules.

Efficient energy storage systems are crucial to address the intermittency of renewable energy sources. As multivalent batteries, Zn-ion batteries (ZIBs), while inherently low voltage, offer a promising low-cost alternative to Li-ion batteries due to the viable use of zinc as the anode. However, to maximize the potential impact of ZIBs, rechargeable cathodes with improved Zn diffusion are needed.

Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway.

We previously reported that manganese (Mn) enhances innate immune responses to viral infection by inducing phosphorylation of TANK-binding kinase 1 (TBK1) in an Ataxia-telangiectasia mutated (ATM)-dependent manner. However, the underlying mechanism by which how Mn induces TBK1 phosphorylation remained unclear. Here, we show that Mn dose-dependently induced TBK1 phosphorylation in the presence of ATM across multiple cell lines, as well as in primary human macrophages and T cells.

Engineering strategies of sequential drug delivery systems for combination tumor immunotherapy.

Over the past few decades, tumor immunotherapy has revolutionized the landscape of cancer clinical treatment. There is a flourishing development of combination strategies to improve the anti-tumor efficacy of mono-immunotherapy. However, instead of a straightforward combination of multiple therapeutics, it is more preferable to pursue a synergistic effect by designing rational combinations as well as administration strategies, which are based on a comprehensive understanding of the physiological and pathological features.

Development and Validation of a Machine Learning-Based Risk Prediction Model for Delirium in Older Inpatients: A Prospective Cohort Study.

Aims: To develop and validate a machine learning-based risk prediction model for delirium in older inpatients.

Design: A prospective cohort study.

Methods: A prospective cohort study was conducted.

Principal component analysis for three-dimensional structured illumination microscopy (PCA-3DSIM).

Three-dimensional structured illumination microscopy (3DSIM) is an essential super-resolution imaging technique for visualizing volumetric subcellular structures at the nanoscale, capable of doubling both lateral and axial resolution beyond the diffraction limit. However, high-quality 3DSIM reconstruction is often hindered by uncertainties in experimental parameters, such as optical aberrations and fluorescence density heterogeneity. Here, we present PCA-3DSIM, a novel 3DSIM reconstruction framework that extends principal component analysis (PCA) from two-dimensional (2D) to three-dimensional (3D) super-resolution microscopy.

Transcriptomic analysis of Lactiplantibacillus plantarum SH7 reveals LuxS/AI-2 quorum sensing-mediated putrescine degradation and enhanced biogenic amine reduction via luxS gene overexpression.

In this study, transcriptomic techniques were used to analyze how 4,5-dihydroxy-2,3-pentanedione (DPD), an exogenous autoinducer-2 (AI-2) precursor, promotes putrescine degradation by Lactiplantibacillus plantarum SH7 through the LuxS/AI-2 quorum sensing (QS) system. The Kyoto Encyclopedia of Genes and Genomes analysis showed that DPD addition up-regulated putrescine-degradation genes and down-regulated putrescine-synthesis genes in L. plantarum SH7, promoting putrescine degradation from both degradation and synthesis aspects.

Monitoring nucleoside metabolism in living cells with a nucleobase analogue fluorescence lifetime imaging.

To overcome challenges in fluorescence labelling of RNA inside living cells we have recently introduced a direct approach using the fluorescent nucleobase analogue 2CNqA. Here we demonstrate its potential for use in fluorescence lifetime imaging (FLIM) to investigate nucleoside metabolism and for metabolic RNA labelling.

A Comprehensive Review on Physical Modifications of Plant Proteins: Mechanism, Influencing Factors, Structural and Functional Properties.

Plant proteins have received increasing attention from food researchers with the growing demand for plant proteins and the emphasis on healthy food and "clean labeling". However, due to their complex and dense structure and greater susceptibility to external factors, plant proteins exhibit poor functional properties, which are not conducive to processing and application in the food industry. Currently, physical modifications of plant proteins have been increasingly reported to improve their functional properties.

Microbial lipases in cheese production: an in-depth review of their role in quality, texture, and flavor.

Cheese, a widely consumed dairy product globally, derives its commercial value primarily from its flavor and textural characteristics that crucially determine consumer acceptance. The quality of cheese including flavor and texture is intricately related to the enzymatic activities of microorganisms during the fermentation process. Among these enzymes, lipases play a pivotal role by hydrolyzing milk fat to generate free fatty acids, which serve as precursors for the formation of key flavor compounds, thereby significantly enhancing the sensory attributes of cheese.

Intelligent deep learning architecture for precision vegetable disease detection advancing agricultural new quality productive forces.

In the context of advancing agricultural new quality productive forces, addressing the challenges of uneven illumination, target occlusion, and mixed infections in greenhouse vegetable disease detection becomes crucial for modern precision agriculture. To tackle these challenges, this study proposes YOLO-vegetable, a high-precision detection algorithm based on improved You Only Look Once version 10 (YOLOv10). The framework incorporates three innovative modules.

An Integrated Approach Using GA-XGBoost and GMM-RegGAN for Marine Corrosion Prediction Under Small Sample Size.

Corrosion is the predominant failure mechanism in marine steel, and accurate corrosion prediction is essential for effective maintenance and protection strategies. However, the limited availability of corrosion datasets poses significant challenges to the accuracy and generalization of prediction models. This study introduces a novel integrated model designed for predicting marine corrosion under small sample sizes.

Study on the Adaptability of FBG Sensors Encapsulated in CNT-Modified Gel Material for Asphalt Pavement.

To prolong the service life of asphalt pavement and reduce its maintenance cost, a fiber Bragg grating (FBG) sensor encapsulated in carboxylated carbon nanotube (CNT-COOH)-modified gel material suitable for strain monitoring of asphalt pavement was developed. Through tensile and bending tests, the effects of carboxylated carbon nanotubes on the mechanical properties of gel materials under different dosages were evaluated and the optimal dosage of carbon nanotubes was determined. Infrared spectrometer and scanning electron microscopy were used to compare and analyze the infrared spectra and microstructure of carbon nanotubes before and after carboxyl functionalization and modified gel materials.

Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection.

Phloem-inhabiting unculturable bacterial pathogens are persistently transmitted by insect vectors. However, how they evade insect immune responses to ensure persistent transmission remains unknown. The important melanization immune response in insects is triggered by cleavage of prophenoloxidase (PPO) into active phenoloxidase (PO) via clip-domain serine proteases (CLIPs).

Multimodal Neural Assessment for Predicting Post-Stroke Dysphagia: Identifying Critical Risk Factors Using Combined Indicators.

BACKGROUND Post-stroke dysphagia (PSD) severely impairs patients' quality of life, yet current diagnostic methods lack efficacy in early detection and prediction. This retrospective study of 300 stroke patients aimed to evaluate risk factors in patients with and without post-stroke dysphagia using functional near-infrared spectroscopy (fNIRS), transcranial magnetic stimulation (TMS), and surface electromyography (sEMG). MATERIAL AND METHODS A total of 300 stroke patients (201 with PSD and 99 without, diagnosed via video fluoroscopic swallowing study or flexible endoscopic evaluation of swallowing) were included.

Enhanced VOC Sensing Performance of Double Transition-Metal MXenes via Pulsed Laser Ablation in Liquid.

MXenes are renowned for their exceptional chemical and physical properties, making them highly promising for gas sensing applications. However, instability remains a significant limitation for these materials. Compared with mono-transition-metal (MTM) MXenes, double transition-metal (DTM) MXenes theoretically offer higher stability and functional diversity.

Microorganism-derived biogenic amine degrading enzymes: mechanisms, molecular modifications, and applications in fermented foods.

Biogenic amines (BAs) threaten food safety and quality. Microorganism-derived BA-degrading enzymes, prized for safety and efficiency, enable BA oxidative degradation in fermented foods. Despite extensive research on enzyme characterization and modification, translating these findings to real fermented food systems for BA reduction remains elusive.