Retrospective analysis of the impact of human papillomavirus infection in the male genital tract on sperm: from a single center.

Objective: This study primarily aimed to investigate human papillomavirus (HPV) infection in males and to evaluate its effect on semen parameters, fertility and partner HPV infection status.

Methods: A total of 624 men who visited the West China Second Hospital of Sichuan University between October 1, 2019, and September 30, 2023, were included. HPV DNA was detected in exfoliated cells from the male genitalia using polymerase chain reaction (PCR) and reverse membrane hybridization to analyze the relationship between HPV infection and semen parameters.

Inhalable Nanovaccine Based on Bioengineered Bacteria-Derived Membrane Vesicles Against Lung Metastasis.

Lung metastases pose a challenge in cancer treatment due to the lung's vascular network and immunosuppressive microenvironment. Conventional subcutaneous vaccines typically fail to elicit localized immune responses at metastatic sites. To address this, an inhalable nanovaccine, BMVax (bacterial membrane-based vaccine), is developed using bacterial membrane vesicles from engineered E.

Acupuncture combined with Tai Chi in the treatment of a patient with coccyx fracture: A case report.

Rationale: Coccyx fractures and subluxations are commonly caused by trauma, often leading to severe pain and restricted mobility. Traditional treatments focus on analgesia and immobilization; however, recovery periods are prolonged. This study explores the efficacy of acupuncture combined with Tai Chi in promoting rehabilitation for coccyx fractures.

A national survey on how to improve the integration of traditional Chinese medicine and artificial intelligence: Attitudes and perceptions from medical staff.

Background: With the significant development of artificial intelligence (AI) in recent years, the inheritance and innovation of traditional Chinese medicine (TCM) urgently require the help of AI technology. The present study was to evaluate the attitudes and perceptions of medical staff towards the integration of TCM and AI development.

Methods: A cross-sectional national survey was conducted at 13 medical institutions across China.

A national survey on the integration of traditional Chinese medicine and artificial intelligence: attitudes and perceptions from the individuals with health needs.

Background: Traditional Chinese medicine (TCM) plays an indispensable role in the healthcare system. Artificial intelligence (AI) opens a new pathway for TCM modernization, while also addressing critical healthcare challenges. The present national survey was conducted to assess the attitudes and perceptions of individuals with health needs regarding the integration of TCM with AI.

Machine Learning Guided Assembly of a Nested Gd@Gd @Ni Cluster via Urea Controlled Carbonate Release.

Lanthanide-based polyhedral clusters are of great interest due to their unique geometries and functional properties, but their controlled synthesis remains a major challenge. Here, we report a nested three-shell cluster, [GdNi(MeIDA)(OH)(CO) (HO)]·(ClO)·(HO) (GdNi, HMeIDA = N-Methyliminodiacetic acid), achieved by using urea as a slow-release carbonate source to direct the formation of a dodecahedral inner shell. Single-crystal X-ray diffraction reveals a unique Gd@Gd@Ni arrangement, with the innermost Gd forming a Platonic dodecahedron templated by carbonate.

Second-order Calibration-assisted Excitation-emission Matrix Fluorescence Detection of Multi-class Mycotoxins in Grape, Wine and Raisin for Food Safety Assessment.

Mycotoxin in grape and its products (wine, raisin) is a widely concerned issue of food safety, it closely associated with consumers' health. In this study, an analytical strategy by combining second-order calibration method with excitation-emission matrix (EEM) fluorescence detection followed photo derivatization (PD) was explored for rapid and sensitive analysis of aflatoxin B (AFB), ochratoxin A (OTA), zearalenone (ZEA) in grape, raisin and wine. Except simple solvent extraction by ethyl acetate and concentration by vacuum distillation, samples don't need other complicated treatment steps any more.

Co-Fermentation of Defatted Zanthoxylum bungeanum Seeds by Lactobacillus casei and Saccharomyces cerevisiae Achieves Complete Alkylamide Degradation and Nutritional Enhancement.

Zanthoxylum bungeanum seeds (ZBS), a nutrient-rich by-product, are underutilized in animal feed due to pungent alkylamides like hydroxy-α-sanshool. This study aimed to degrade alkylamides via microbial fermentation and enhance ZBS's nutritional value. Lactobacillus casei 37 and L.

Establishing the Minimal Clinically Important Difference, Substantial Clinical Benefit, and Patient Acceptable Symptomatic State for Patient-Reported Outcome Measures After Primary Posterior Cruciate Ligament Reconstruction With a Minimum 5-Year Follow-up.

Background: Improvements in patient-reported outcome measure (PROM) scores have been widely documented after posterior cruciate ligament reconstruction (PCLR). Establishing clinically significant outcome (CSO) benchmarks for PROMs can enhance their clinical applicability.

Purpose: To establish the minimal clinically important difference (MCID), substantial clinical benefit (SCB), and patient acceptable symptomatic state (PASS) for PROMs after primary PCLR and identify the predictors of achieving them.

Dual-functional DNA nanoribbon-templated copper nanoclusters synergistically activate NRF2/HO-1 pathway for synergistic oxidative stress mitigation.

Employing antioxidant nanozymes to eliminate reactive oxygen species (ROS) is a promising strategy for alleviating oxidative stress. However, most current nanozymes struggle to balance catalytic efficacy with biosafety, limiting their clinical applicability. In this study, we introduce a novel platform: DNA nanoribbon-templated copper nanoclusters (DNR/Cu NCs), which harness dual antioxidative mechanisms (direct ROS scavenging and activation of nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway) to synergistically mitigate oxidative stress.

Ultrabroadband on-chip photonics for full-spectrum wireless communications.

The forthcoming sixth-generation and beyond wireless networks are poised to operate across an expansive frequency range-from microwave, millimetre wave to terahertz bands-to support ubiquitous connectivity in diverse application scenarios. This necessitates a one-size-fits-all hardware solution that can be adaptively reconfigured within this wide spectrum to support full-band coverage and dynamic spectrum management. However, existing electrical or photonic-assisted solutions face a lot of challenges in meeting this demand because of the limited bandwidths of the devices and the intrinsically rigid nature of system architectures.

A Sponge-like Polysaccharide from Pine Pollen: Structural Features and Therapeutic Potential in DNCB-Induced Atopic Dermatitis Models.

Atopic dermatitis (AD) is a long-term, recurring inflammatory skin condition characterized by impaired epidermal barrier function and abnormal immune system regulation. Pine pollen has traditionally been used for dermatological treatments, though its active components remain unclear. The primary objective of this study was to pinpoint the active constituents of pine pollen and elucidate its therapeutic effects against AD.

Residual compensation algorithm for machine learning-based LIBS quantification optimization.

We propose a laser-induced breakdown spectroscopy (LIBS) quantitative prediction algorithm based on residual compensation that can effectively reduce the mean absolute error of prediction (MAEP) and mean relative error of prediction (MREP) by incorporating environmental and sample parameters into the prediction model. A 10-fold cross-validation of the residual compensation model based on four common models, support vector machine regression (SVR), partial least squares regression (PLSR), random forest regression (RFR), and K-nearest neighbor regression (KNNR), was applied to test the aluminum alloy samples. After testing on 10 elements, MAEP and MREP were reduced by an average of 51.

Integrating bulk and single-cell sequencing reveals cellular heterogeneity between lung adenocarcinoma in smokers and never-smokers.

Lung cancer in smokers (LCIS) and lung cancer in never-smokers (LCINS) are different entities with distinct molecular features. However, their cellular heterogeneity still requires further investigation. Through an integrated analysis of single-cell RNA sequencing (scRNA-seq) and bulk sequencing data, we identified cell subpopulations associated with smoking and non-smoking patients.

Analysis and prediction of cardiovascular research hotspots, trends and interdisciplinarity.

Objective: Comprehensive data and analyses on cardiovascular research could clarify recent research trends for the academic community and facilitate policy development. We examined publications and reference data to identify research topics, trends and interdisciplinarity for cardiovascular disease (CVD).

Methods: We extracted and clustered text fragments from the titles and abstracts of 2 512 445 publications using artificial intelligence techniques, including natural language processing (NLP) for semantic analysis.

Topology-Optimized 3D Metalloporphyrin Covalent Organic Framework for Photocatalytic CO Fixation.

Three-dimensional (3D) covalent organic frameworks (COFs) represent promising photocatalytic platforms with accessible active sites, yet their development faces challenges in terms of structural diversity and synthetic complexity. Herein, a robust 3D metalloporphyrin COF with topology and 2-fold interpenetration is achieved by using high-connectivity metalloporphyrin, and different metal centers within the porphyrin units of 3D COFs lead to intriguing structural variations. Particularly, the 3D cobalt-porphyrin-based COF achieves efficient photocatalytic CO-to-CO conversion with a rate of 21 251.

Natural history outcome of moderate tricuspid regurgitation with preserved left-ventricular ejection fraction.

Background: The long-term natural history of moderate tricuspid regurgitation (TR) patients with preserved left-ventricular ejection fraction (LVEF) and without severe left-sided valvular heart disease (VHD) remains ambiguous. We aimed to assess the outcomes of patients with moderate TR, preserved LVEF and without concomitant severe left-sided VHD.

Methods: We evaluated patients diagnosed with moderate TR in our centers between 2012 and 2020.

Differential tropisms of old and new world hantaviruses influence virulence and developing host-directed antiviral candidates.

Hantaviruses are zoonotically transmitted from rodents to humans through the respiratory route, with no currently approved antivirals or widely available vaccines. The recent discovery of interhuman-transmitted Andes virus (ANDV) necessitates the systematic identification of cell tropism, infective potential, and potent therapeutic agents. We utilized human primary lung endothelial cells, various pluripotent stem cell-derived heart and brain cell types, and established human lung organoid models to evaluate the tropisms of Old World Hantaan (HTNV) and New World ANDV and Sin Nombre (SNV) viruses.

Branched-chain amino acids contribute to diabetic kidney disease progression via PKM2-mediated podocyte metabolic reprogramming and apoptosis.

Approximately 30-40% of patients with diabetes develop diabetic kidney disease (DKD). Identifying decisive factors for DKD initiation is crucial. Here, we observed that glomerular podocytes in male and female patients with DKD and db/db mice specifically displayed BCAA catabolic defects.

3D Printing of Conductive Polymer via Photo-Oxidation Polymerization inside Metal-Organic Framework.

Despite the crucial role of conductive polymers in flexible microelectronics, current 3D fabrication of conductive polymers is plagued by solubility problems and low precision. Femtosecond laser direct writing (FsLDW) offers a potential solution, yet complex 3D structures constructing of conductive polymers remains challenging. Here, an innovative approach that utilizes inorganic microporous metal-organic framework (MOF) crystals as supporting substrates for 3D printing of polypyrrole (PPy) is developed.

Modified laparoscopic transvesical diverticulectomy: a case series study for the treatment of giant bladder diverticulum.

BACKGROUND​: Bladder diverticulum presents significant surgical challenges in traditional laparoscopic approaches, including difficulty in locating diverticular openings and risks of ureteral injury. This study introduces a novel "modified laparoscopic transvesical diverticulectomy" featuring an inside-out approach with CO gas bladder insufflation, addressing these limitations and offering an innovative minimally invasive solution for giant bladder diverticula. CASE SERIES​: Between 2022 and 2024, a case series of 10 patients (9 males, 1 female; mean age 70.

The associations between pancreatic fat infiltration and its combination with the triglyceride glucose index and the risk of coronary calcification: A multicenter study.

Introduction: Intrapancreatic fat deposition is related to insulin resistance and type 2 diabetes mellitus. However, the association between intrapancreatic fat deposition and coronary artery disease has not been well studied. In this study, we investigated the associations between intrapancreatic fat deposition alone or in combination with triglyceride glucose index (TYG) and the risk of coronary artery calcification (CAC) in a general population.

Analysis of the short-to-intermediate-term efficacy of autologous costochondral transplantation in treatment for Hepple V osteochondral lesion of talus.

Objectives: To analyze the feasibility, effectiveness and safety of autologous costochondral transplantation (ACT) in patients with Hepple V osteochondral lesion of talus (OLT), and to analyze the short-to-intermediate-term efficacy.

Methods: Clinical data of 27 patients (27 ankles) with Hepple V OLT admitted in Shanghai Jiao Tong University School of Medicine Affiliated Sixth People's Hospital between February 2020 and February 2022 were retrospectively analyzed, of which were treated with ACT. The general information, operation time, and hospitalization days of the patients were recorded.

Multi-axis compression fusion network for vehicle re-identification.

Vehicle re-identification (Re-ID) has become a challenging retrieval task due to the high inter-class similarity and low intra-class similarity among vehicles. To address this challenge, the self-attention mechanism has been extensively studied and applied, demonstrating its effectiveness in capturing long-range dependencies in vehicle Re-ID. Traditional spatial self-attention and channel self-attention assign different weights to each node (position/channel) based on pairwise dependencies at a global scale to model long-term dependencies, but this approach is not only computationally complex but also unable to fully mine refined features.

Towards the elimination of infectious HPV: exploiting CRISPR/Cas innovations.

HPV has been conclusively associated with various human malignancies, making the development of prevention and treatment strategies for HPV-induced diseases a high priority. Currently, primary prevention methods include HPV immunization and routine screening, which significantly reduce the risk of HPV transmission. However, for patients diagnosed with invasive, advanced, or recurrent malignancies, non-virus-specific therapies frequently lead to drug resistance and adverse effects, resulting in minimal improvement in treatment efficacy for numerous patients.