MLKL PARylation in the endothelial niche triggers angiocrine necroptosis to evade cancer immunosurveillance and chemotherapy.

Chemoresistance is the leading cause of cancer-related death. How chemotherapy subjugates the cellular crosstalk in the tumour microenvironment to cause chemoresistance remains to be defined. Here we find chemotherapy enables immunosuppressive SDF1 endothelial niche to evade immunosurveillance in ovarian and breast cancers.

Midterm outcomes of rheumatic mitral valve repair versus replacement in a dual-centre retrospective study.

Background: Rheumatic mitral valve disease remains a major global health challenge. Determining optimal surgical approaches is critical. This study aimed to identify key repairability factors and compare midterm outcomes of mitral repair versus replacement.

Habitat Suitability Evaluation of Chinese Red Panda in Daxiangling and Xiaoxiangling Mountains.

The Chinese red panda () is a rare and endangered animal in China; the increase in global temperature and the interference of human activities have caused irreversible effects on the suitable habitat of wild red pandas and threatened their survival. Therefore, it is necessary to carry out scientific research and protection for Chinese red pandas. In this study, the MaxEnt model was used to predict and analyze the suitable habitats of Chinese red pandas in the large and small Xiangling Mountains.

DiffRaman: A conditional latent denoising diffusion probabilistic model for enhancing bacterial identification via Raman spectra generation under limited data.

Raman spectroscopy has attracted significant attention in various biochemical detection fields, especially in the rapid identification of pathogenic bacteria. The integration of this technology with deep learning to facilitate automated bacterial Raman spectroscopy diagnosis has emerged as a key focus in recent research. However, the diagnostic performance of existing deep learning methods largely depends on a sufficient dataset, and in scenarios where there is a limited availability of Raman spectroscopy data, it is inadequate to fully optimize the numerous parameters of deep neural networks.

Advances on the biosynthesis of ergothioneine using microbial chassis.

Ergothioneine (ERG) is a naturally occurring sulfur-containing amino acid with a unique structure. Due to its potent antioxidant properties and diverse biological functions, ERG has attracted increasing attention from researchers and is now widely used in the food, cosmetics, and pharmaceutical industries. The primary methods for obtaining ergothioneine include biological extraction, chemical synthesis, and biosynthesis.

[Role of traditional Chinese medicine concepts in the prevention of childhood caries].

The high prevalence of childhood caries poses a considerable threat to children's overall health. Traditional Chinese medicine (TCM) concepts offers a distinctive and valuable perspective on caries prevention. This study explores TCM's conceptualization of dental caries in relation to the physiological characteristics of children and proposes targeted preventive strategies, including holistic care, dietary regulation, and lifestyle modification.

Establishing a long-term predictive model for aggressive behavior in schizophrenia: a 21-year longitudinal study in rural China.

Background: Although identifying factors contributing to aggressive behavior in individuals with schizophrenia is crucial for developing targeted prevention strategies and intervention, most studies were cross-sectional or short-term, and did not take into account the factor of urbanicity. This study aimed to develop a predictive model of aggressive behavior in individuals with schizophrenia in rural China.

Method: A total of 205 individuals with schizophrenia who were identified in 1994 and followed up in 2015 were included in the study.

A cross-sectional analysis of fingerstick blood self-microcollection for remote HIV suppression monitoring in Atlanta, Georgia, USA: a path to expanding access to continuum of care.

Background: Many people with HIV experience considerable barriers to accessing HIV clinic services. Options that would permit blood sampling that preclude the need for in-clinic visits and increase privacy would aid in overcoming many of the obstacles that hinder receiving adequate HIV care.

Methods: In Project Home-MaDE, 57 participants were evaluated for their ability to collect fingerstick blood (minimum 250 µL) in Microtainer tubes (MCT), then package and overnight mail specimens following kit instructions without assistance.

Discovery of a highly selective BChE inhibitor with concomitant anti-neuroinflammatory activity for treating Alzheimer's disease.

Recently, butyrylcholinesterase (BChE) has emerged as a potential therapeutic target for advanced Alzheimer's disease (AD). Herein, 20 novel carbamate-based N-benzoyl tryptamine derivatives were designed and synthesized by integrating a BChE-targeting fragment with the original carrier scaffold, which possesses good anti-neuroinflammatory activity. Among them, N14 was proved to be the most potent and highly selective BChE inhibitor [eqBChE IC = 18.

2D magnetotelluric forward modeling based on multitask deep learning.

The accuracy and efficiency in 2D magnetotelluric (MT) forward modeling determine inversion quality. Traditional numerical methods, while achieving reliable results on high-performance computing clusters, face challenges of heavy computational burden and inefficiency when implemented on personal computers due to their computationally intensive nature, this study proposes a novel 2D MT forward modeling method based on a Transformer U-Net (T-Unet) multitask network. Through end-to-end training, the network establishes a mapping relationship between geoelectric models and apparent resistivity as well as phase, generates corresponding datasets, and obtains a neural network weight model capable of directly predicting MT forward modeling results after training.

Inhibition of USP46/ANK2 Axis Alleviates Myocardial Infarction in Hypoxia/Reoxygenation-Treated Cardiomyocytes and Ischemia/Reperfusion-Induced Rat Models.

Ankyrin2 (ANK2) has been found to be abnormally overexpressed in myocardial infarction (MI) cell models, but its role and related mechanisms in MI progression have not been explored. Cardiomyocytes (AC16) were subjected to hypoxia/reoxygenation (H/R) treatment to mimic MI cell models, and ischemia/reperfusion (I/R)-induced myocardial injury was used to establish MI rat models. Cell viability, apoptosis and mitochondrial membrane potential (MMP) depolarization were analyzed by CCK8 assay, flow cytometry and JC-1 staining.

Integrative Multi-Omics Analysis Reveals Key Metabolic Regulators and Prognostic Biomarkers in Pediatric and Adult Thyroid Cancer.

Thyroid cancer, including papillary thyroid carcinoma (PTC) and anaplastic thyroid carcinoma (ATC), exhibits distinct molecular characteristics in adult and pediatric populations. Understanding these differences is vital for identifying therapeutic targets and prognostic biomarkers. We performed an integrative multi-omics analysis combining proteomics, phosphoproteomics, metabolomics, and RNA sequencing data from adult and pediatric thyroid cancer cohorts.

Activated Interferon-γ-Positive T Lymphocytes and Cytokine Signatures in Patients With Postinfectious Cough.

Postinfectious subacute cough (PISC) and postinfectious chronic cough (PICC) are triggered by respiratory infections, which induce adaptive immunity. The expression of T-lymphocyte subsets and cytokine signatures remains elusive in these patients. Here, we recruited 40 healthy controls, 64 PICC patients, 65 PISC patients, and 20 recovered individuals with postinfectious subacute cough (R-PISC).

Machine-Learning Models With Multiple Imputation With Sequential Nearest Neighbors Imputation for Predicting the Prognosis of Idiopathic Sudden Sensorineural Hearing Loss Patients.

Objectives: The prognosis of idiopathic sudden sensorineural hearing loss (ISSNHL) is heterogeneous. The study aimed to investigate the prognostic factors of ISSNHL and develop machine-learning models with multiple imputation to predict the prognosis of ISSNHL.

Design: A retrospective study was undertaken on a cohort of 600 patients with ISSNHL who underwent standardized treatment protocols.

A comprehensive understanding of thermal tolerance in Percocypris pingi (Tchang, 1930) under different acclimation temperatures supported by behavioral assessments.

This study assessed the thermal limits of Percocypris pingi, initially reared at 9.86 °C. Fish were short-term acclimated to 12 °C, 18 °C, and 24 °C for two weeks, and their CT and CT were determined.

The relationship between different missed care activities and patient outcomes: A systematic review.

Background: Missed care is a key indicator of nursing quality and has been linked to negative patient outcomes. However, the rates of missed care vary across different nursing activities. Treating missed care as a composite indicator may lack specificity in relation to patient outcomes.

Public Attention to Mpox in China During the Pandemic: Qualitative Analysis of TikTok Data Using Latent Dirichlet Allocation Topic Modeling.

Background: Mpox has reemerged as a global public health concern. With the growing reliance on social media for health information dissemination, understanding public perception through these platforms is essential for designing effective health promotion strategies.

Objective: This study analyzes TikTok data related to mpox using Latent Dirichlet Allocation (LDA) topic modeling.

Natural Variations in the Promoter of CHILLING TOLERANCE DIVERGENCE 8 Contribute to the Functional Divergence in the Chilling Tolerance of Rice.

Improvement of the chilling tolerance is one of the key points in rice breeding since it is highly sensitive to chilling stress, which often limits rice production. Here, we identified a quantitative trait locus for chilling tolerance, qCTS8, from a recombinant inbred line (RIL) population derived from crossing Dongxiang wild rice (DXWR) with Guangluai 4 (GLA4). Map-based cloning revealed CHILLING TOLERANCE DIVERGENCE 8 (COLD8) as the underlying major gene.

Enzymatic hydrolysis combined with chemiluminescence immunoassay: Foresight for automated and rapid urinary aldosterone detection.

Background: The 24-hour urinary aldosterone test offers superior stability in assessing aldosterone secretion compared to single-point plasma measurements, suggesting its potential utility as a diagnostic tool for primary aldosteronism. However, its clinical utility is hampered by labor-intensive manual urine treatment and the lack of diagnostic cut-off, with the former demonstrating considerable inter-laboratory variability. There is an urgent need for an automated assay to facilitate rapid and standardized 24-hour urinary aldosterone testing, accompanied by method-specific diagnostic cut-offs.

CuSO protects hepatocytes from lipotoxicity by promoting autophagic degradation of lipid droplets to attenuate oxidative stress.

Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) is a prevalent chronic liver condition that often progresses to Metabolic Dysfunction-Associated Steatohepatitis (MASH), ultimately leading to liver fibrosis and hepatocellular carcinoma (HCC). Consequently, the early diagnosis and treatment of MASLD are particularly crucial. Currently, the pathogenic mechanisms underlying MASLD remain incompletely understood, and there is no optimal therapy available for this condition.

Variations in executive function in toddlers after exposure to pre-pregnancy overweight and obesity and the mediating role of inflammation in a longitudinal pregnancy cohort study.

Objective: This study aimed to determine the association between pre-pregnancy overweight and obesity status in pregnant women and executive function (EF) development in toddlers and establish whether inflammatory biomarkers mediate this association.

Methods: This prospective cohort included 1,126 mother-infant pairs. Pre-pregnancy body mass index (ppBMI) was obtained from medical records, and the participants categorized into normal weight, overweight and obese groups according to standard ppBMI classifications.

PTEN restricts IL-21R signaling in GC B cells and suppresses their differentiation to plasma cells.

The germinal center (GC) reaction is essential for generating high-quality humoral memory. Positively selected GC B cells must decide whether to remain in the GC for further affinity maturation or differentiate into memory or plasma cells (PCs). Previously, we identified IL-21R and CD40 signaling as critical promoters of GC B cell effector differentiation.