Eicosapentaenoic acid restores inflammation-induced changes in chondrocyte mechanics by suppressing the NF-κB p65/CD44 signaling pathway and attenuates osteoarthritis.

Here we investigate the effects and mechanisms of eicosapentaenoic acid (EPA) in regulating chondrocyte mechanics during inflammation in the progression of osteoarthritis (OA). Primary porcine chondrocytes and human OA chondrocytes were isolated and cultured. Cell mechanical properties were measured using atomic force microscopy.

Discovery of a highly selective BChE inhibitor with concomitant anti-neuroinflammatory activity for treating Alzheimer's disease.

Recently, butyrylcholinesterase (BChE) has emerged as a potential therapeutic target for advanced Alzheimer's disease (AD). Herein, 20 novel carbamate-based N-benzoyl tryptamine derivatives were designed and synthesized by integrating a BChE-targeting fragment with the original carrier scaffold, which possesses good anti-neuroinflammatory activity. Among them, N14 was proved to be the most potent and highly selective BChE inhibitor [eqBChE IC = 18.

Self-healing and adhesive eutectogels with dual conductive networks for multimodal health monitoring and human-computer interaction.

Flexible sensors exhibit transformative potential across diverse applications ranging from continuous health monitoring to advanced human-machine interaction. However, conventional unimodal sensors are limited in their ability to capture multidimensional signals in complex scenarios. To address this issue, this study synthesized a polymerizable deep eutectic solvent (pDES) from choline chloride (Chcl) and acrylic acid (AA).

Reliability and validity of the Standardized swallowing assessment among community-dwelling older adults in China.

Background: Oropharyngeal Dysphagia (OD) has become a public health issue and early screening has practical significance. The Standardized Swallowing Assessment (SSA) is a clinician-driven, simple, and efficient screening tool but has yet to be validated in Chinese communities.

Objectives: This study aimed to cross-culturally adapt and validate the SSA in Chinese community-dwelling older adults and explore its optimal cut-off value.

Pediatric metabolic dysfunction-associated steatotic liver disease and the gut microbiome: from research landscape to targeted modulation.

Metabolic dysfunction-associated steatotic liver disease (MASLD), formally known as non-alcoholic fatty liver disease, has become the most common form of chronic liver disease in children. The spectrum of pediatric MASLD ranges from simple steatosis to steatohepatitis, fibrosis, cirrhosis, and in rare cases, hepatocellular carcinoma. Its pathogenesis involves a complex interplay among genetic, epigenetic, and environmental factors, along with alterations in the gut microbiota and its associated metabolites.

Integrative Omics Reveals Glutamine Catabolism-Driven Apoptotic Suppression in Monocytes upon Mechanical Unloading.

Osteoclasts derived bone marrow monocytes have been documented to modulate bone quality by directly sensing mechanical forces. However, the mechanisms by which osteoclasts perceive and respond to mechanical disturbances remain unclear. Through integrating multi-omics data of bone tissues from hindlimb unloading (HLU) and control mice, it is revealed that glutamine (Gln) catabolism-induced suppression of apoptosis is critical for monocytes sensing and responding to mechanical unloading.

Refining the Interaction Between Social Norms and Uncertainty About Risk Perceptions in Health and Environmental Behaviors- A Cross-Cultural Replication and Extension.

Social norms play a crucial role in shaping risk-mitigating behaviors, prompting extensive research to advance theory, and inform practices. However, until recently, limited attention has been given to how individuals' (meta)cognitive appraisals of risks may interact with social norms to affect risk-mitigating behaviors. Expanding previous work, this study employs a cross-sectional survey ( = 1,249) to (1) further differentiates from , (2) test the interaction between two types of social norms and (uncertainty about) risk perceptions, and (3) replicate the findings across two cultural contexts (i.

Impact of Acute Total Occlusion of the Culprit Vessel on Prognosis and Risk Stratification in Patients With Non-ST-Segment Elevation Myocardial Infarction.

Non-ST-segment elevation myocardial infarction (NSTEMI) is a common form of acute coronary syndrome (ACS), and approximately 25% of patients present with acute total occlusion (ATO) of the culprit vessel. ATO may lead to more extensive myocardial injury and poorer outcomes. However, its prognostic significance in NSTEMI, particularly in the Chinese population, is not well established.

Embedding narrative medicine in primary healthcare: Exploration and practice from a medical humanities perspective.

This study examines the integration of narrative medicine (NM) into primary healthcare (PHC) settings, evaluating its role in enhancing medical humanities education within grassroots healthcare institutions. Through a comprehensive literature review and case analysis, the research investigates the current state, challenges, and practical barriers to embedding NM into PHC systems, while proposing targeted strategies for improvement. The findings suggest that NM fosters stronger doctor-patient trust, enhances healthcare quality, and promotes humanistic care.

Construction and Validation of a Nomogram Prediction Model for the Risk of Cefoperazone Sodium/Sulbactam Sodium-Related Coagulation Disorders.

Objective: To construct and validate a nomogram prediction model for the risk of cefoperazone sodium/sulbactam sodium (CPZ/SAM)-related coagulation disorders in hospitalized patients.

Methods: Patients treated with CPZ/SAM from January 2022 to December 2024 were enrolled and divided into a coagulation disorder group and a control group based on the occurrence of coagulation disorders. Clinical data were collected to identify risk factors and construct a nomogram model, which was validated using the Hosmer-Lemeshow goodness-of-fit test, receiver operating characteristic (ROC) curve, decision curve analysis (DCA), and clinical impact curve.

Immune microenvironment heterogeneity characterizes biologically distinct KRAS/SPOP and KRAS/PIK3CA mesonephric-like adenocarcinoma subtypes revealed by integrated whole-exome and transcriptomic profiling.

Objective: This study aims to uncover the molecular biology and immune microenvironment of gynecological mesonephric-like adenocarcinoma (MLA).

Methods: To determine the comprehensive characteristics of MLA, 17 patients with MLA were retrospectively enrolled in this study. Whole-exome sequencing and mRNA sequencing were performed to explore the molecular features.

Aramchol attenuates fibrosis in mouse models of biliary fibrosis and blocks the TGFβ-induced fibroinflammatory mediators in cholangiocytes.

Background: Cholestatic liver diseases, including primary sclerosing cholangitis, are characterized by biliary fibroinflammation. TGFβ-activated cholangiocytes release signals that recruit immune cells and activate myofibroblasts, promoting inflammation and extracellular matrix (ECM) deposition. TGFβ also regulates stearoyl-CoA desaturase (SCD), an enzyme involved in lipid signaling.

Screening for alcohol dehydrogenase inhibitors in natural compounds by electrophoretically mediated microanalysis.

Alcohol dehydrogenase (ADH), a pivotal enzyme in ethanol metabolism, has gained prominence as a promising therapeutic target. This study developed an electrophoretically mediated microanalysis (EMMA) platform integrated with rapid polarity switching to enable efficient mixing of reactants and rapid screening of ADH inhibitors from natural compounds. Systematic optimization of capillary electrophoresis separation and enzymatic reaction parameters yielded precise kinetic characterization, with a Michaelis constant (K) of 2.

[Research progress on mechanism of traditional Chinese medicine in treatment of premature ovarian insufficiency].

Premature ovarian insufficiency(POI) is a manifestation of ovarian aging, with a global incidence of 3.5%. If not addressed in time, POI can rapidly develop into premature ovarian failure(POF).

Dosimetric evaluation of field parameters in VMAT for cervical cancer with inguinal lymphatic drainage extension.

For cervical cancer radiotherapy, patients requiring inguinal lymphatic drainage (ILD) extension fields pose challenges due to the large and complex target volume. To investigate the dosimetric effects of x-jaw width, jaw aperture position, and the number of arcs on volumetric modulated arc therapy (VMAT) plans for ILD extension fields, in order to optimize treatment planning. Thirty-nine patients were retrospectively included, and seven VMAT planning groups were created: OF (open field), X = 15/16/17/18 (jaw widths of 15-18 cm), SP (split-field, four arcs), and X = 15 M (jaw aperture moved toward the isocenter).

Sociotechnical imaginaries and public communication: Analytical framework and empirical illustration using the case of artificial intelligence.

The concept of sociotechnical imaginaries (SIs) has been widely used and proven fruitful to understand diverging trajectories of technologies. While scholars have acknowledged the multi-layered materialisation of SIs and highlighted the importance of the communicative layer therein, this aspect has remained under-conceptualised. Therefore, we propose an analytical framework to better understand Sociotechnical Imaginaries in Public Communication (SIPCs), defined as constructed visions of (un)desirable sociotechnical futures that guide action, mobilise resources and lay out trajectories for the materialisation or prevention of those futures.

Deguelin's Anticancer Bioactivity: Challenges and Opportunities in Medicinal Chemistry.

Deguelin is a natural isoflavone derived from the rotenone family, commonly found in plants belonging to the Derris and Grifola genera. Comprehensive research has underscored its considerable promise in oncological treatment. Deguelin demonstrates anticancer activity by suppressing cell proliferation and promoting apoptosis the modulation of critical signaling pathways, such as NF-κB, Wnt, and AMPK pathways.

Analysis of trends and hotspots in the research on sleep disorders of maintenance hemodialysis patients based on bibliometrics.

Objective: To explore the research hotspots and frontiers of sleep disorders in patients undergoing maintenance hemodialysis (MHD) over the past 15 years.

Methods: Relevant literature published from January 1, 2010, to April 1, 2025, was retrieved from the Web of Science Core Collection database. Bibliometric analysis and VOSviewer software were employed to visualize data on countries, institutions, journals, authors, and keywords.

Relationship between stress hyperglycemia ratio and the incidence of atrial fibrillation in patients after coronary artery bypass grafting: a retrospective study based on the MIMIC-IV database.

Background: The stress hyperglycemia ratio (SHR) is a clinical index that quantifies acute stress-induced hyperglycemia by comparing immediate blood glucose levels with chronic glucose control (reflected by HbA1c). It is especially valuable in cardiovascular disease and surgical prognosis. However, there is still a lack of research on the relationship between SHR and new-onset atrial fibrillation (AF) in patients after coronary artery bypass grafting (CABG).

Huaganjian decoction ameliorates metabolic dysfunction-associated steatotic liver disease through liver-gut crosstalk.

Ethnopharmacological Relevance: Huaganjian decoction (HGJD), a traditional Chinese medicine formula, has been widely acknowledged for its remarkable effects in soothing the liver, relieving depression, and alleviating hepatic inflammation. Clinically, it has been extensively applied in the treatment of metabolic dysfunction-associated steatotic liver disease (MASLD). Nevertheless, the underlying mechanisms of its therapeutic actions remain incompletely understood, thus necessitating further in-depth investigations.

Deciphering the MHC immunopeptidome of human cancers with Ligand.MHC atlas.

A fundamental principle of immunotherapy is that T cells are capable of detecting tumor epitopes presented on cancer cell surfaces. Immunopeptidomic strategies empowered by liquid chromatography-tandem mass spectrometry have transformed tumor epitopes identification and provided novel insights into tumor immunology. It enables in-depth profiling of major histocompatibility complex (MHC) presented ligands, thereby offering valuable perspectives on the molecular dialog among tumor and T cells.

Geographic Origin and Functional Group Type Affect the Decomposability of Peatland Plant Litter Through Biochemical Properties.

Peatlands are the most important global soil carbon reservoirs due to low decomposition rates. However, the influence of peatland plant litter quality and geographic environment on decomposition remains poorly understood. This article aims to explore the interactive effects of geographical origin and plant functional groups on the initial chemical characteristics and decomposition processes of peatland litter, elucidating how climate-driven biochemical legacies regulate decomposition dynamics.

Validation of guiding needle placement using registered ultrasound imaging for gynecologic brachytherapy in a simulated pelvic Phantom.

Our previous research demonstrated that, under ideal conditions, high-precision image registration between real-time ultrasound (US) images and preoperative CT/MR images could be achieved using real-time US guidance for needle insertion. In this study, we enhanced a constructed phantom to better simulate the structure of human abdominal tissue, aiming to verify the accuracy of surgical needle placement guided by real-time US images registered with preoperative CT/MR images. Specifically, the pelvic phantom was improved using animal abdominal tissue to mimic the properties of human abdominal tissue.