Investigation into Antibiotic Resistance Profiles of Among Chronic Rhinosinusitis Patients and Multifaceted Analysis of Factors Influencing Methicillin-Resistant Infection.

Objective: This study aimed to comprehensively investigate the antibiotic resistance characteristics of in chronic rhinosinusitis (CRS) patients and to identify key determinants influencing the development of methicillin-resistant Staphylococcus aureus (MRSA) infections.

Methods: A retrospective analysis was conducted on 180 CRS patients admitted to our hospital between February 2022 and July 2024. Nasal secretion samples were collected upon admission for strain isolation, and antibiotic susceptibility testing was performed using an automated microbiology system.

Glyphosate bioremediation using a newly isolated Bacillus albus strain F9D: mechanisms and kinetic studies.

Unlabelled: Glyphosate is widely used as an herbicide around the world. The extensive application of glyphosate, however, has serious adverse effects on living systems. Therefore, the elimination of residual glyphosate pollution has become an urgent issue worldwide.

Integrative Omics Reveals Glutamine Catabolism-Driven Apoptotic Suppression in Monocytes upon Mechanical Unloading.

Osteoclasts derived bone marrow monocytes have been documented to modulate bone quality by directly sensing mechanical forces. However, the mechanisms by which osteoclasts perceive and respond to mechanical disturbances remain unclear. Through integrating multi-omics data of bone tissues from hindlimb unloading (HLU) and control mice, it is revealed that glutamine (Gln) catabolism-induced suppression of apoptosis is critical for monocytes sensing and responding to mechanical unloading.

Analysis of the Clinical Symptom Improvement and Recurrence Rate in Chronic Suppurative Otitis Media Patients Treated with Combination Therapy: Combination of Ofloxacin and Dexamethasone.

Objective: To analyze the clinical symptom improvement and recurrence rate of chronic suppurative otitis media (CSOM) patients treated with a combination therapy regimen (combination of ofloxacin and dexamethasone).

Methods: Based on the treatment regimen, patients were divided into two groups: the control group was treated with dexamethasone, while the treatment group received a combination of ofloxacin and dexamethasone. The clinical symptom improvement, hearing evaluation, pathogen clearance rate, inflammatory marker detection, adverse reactions, recurrence rate and recurrence rate post-treatment were compared between the two groups.

Investigation on the Distribution of in Yuanshui Unique Fish Species National Aquatic Germplasm Resources Reserve Using Environmental DNA Technology.

Freshwater ecosystems face unprecedented challenges as the cumulative impact of human activities intensifies. While protected areas and species-specific conservation policies are widely implemented, their effectiveness remains difficult to gauge using traditional catch-based surveys. This research employed environmental DNA (eDNA) technology to assess the distribution of the endangered fish, , within the Yuanshui Unique Fish Species National Aquatic Germplasm Resources Reserve (YUFRR) in Hunan, China.

Multi-parameter information detection of aircraft taxiing on an airport runway based on an ultra-weak FBG sensing array.

Tracking the moving state and analyzing the distribution feature of an aircraft taxiing on the pavement are crucial for runway safety management. One challenge is to provide a reliable methodology that would enable such needs to be addressed in long-distance range runway with high sensitivity and spatial resolution. This paper proposed a novel monitoring approach for acquiring multi-parameter information of aircraft taxiing on airport runway based on distributed vibration detected by ultra-weak fiber Bragg grating (FBG) sensing array.

Microorganism-Driven 2,4-D Biodegradation: Current Status and Emerging Opportunities.

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has been widely used around the world in both agricultural and non-agricultural fields due to its high activity. However, the heavy use of 2,4-D has resulted in serious environmental contamination, posing a significant risk to non-target organisms, including human beings. This has raised substantial concerns regarding its impact.

The Discovery of a Specific CKIP-1 Ligand for the Potential Treatment of Disuse Osteoporosis.

Bone homeostasis relies on the delicate balance between osteoblast-mediated bone formation and osteoclast-mediated bone resorption. The casein kinase 2 interacting protein-1 (CKIP-1), a specific CK2α subunit-interacting protein, has been documented as one of the crucial negative regulators of bone formation. CKIP-1 siRNA therapy has constraints that limit its use in clinical applications.

Targeting Smurf1 to block PDK1-Akt signaling in KRAS-mutated colorectal cancer.

The phosphoinositide 3-kinase (PI3K)-Akt axis is one of the most frequently activated pathways and is demonstrated as a therapeutic target in Kirsten rat sarcoma viral oncogene homolog (KRAS)-mutated colorectal cancer (CRC). Targeting the PI3K-Akt pathway has been a challenging undertaking through the decades. Here we unveiled an essential role of E3 ligase SMAD ubiquitylation regulatory factor 1 (Smurf1)-mediated phosphoinositide-dependent protein kinase 1 (PDK1) neddylation in PI3K-Akt signaling and tumorigenesis.

The complete mitochondrial genome of Zhu and Cao 1988 (Cypriniformes: Nemacheilidae).

The genus is an ideal taxon for understanding geological evolution. In this study, for the first time, we report the complete mitochondrial genome of Zhu and Cao 1988 using the Nanopore sequencing. It is a circular genome with a length of 16558 bp, comprising 22 tRNAs, 13 protein-coding genes (PCGs), two rRNAs, and one non-coding control region.

Cepharanthine Ameliorates Pulmonary Fibrosis by Inhibiting the NF-κB/NLRP3 Pathway, Fibroblast-to-Myofibroblast Transition and Inflammation.

Pulmonary fibrosis (PF) is one of the sequelae of Corona Virus Disease 2019 (COVID-19), and currently, lung transplantation is the only viable treatment option. Hence, other effective treatments are urgently required. We investigated the therapeutic effects of an approved botanical drug, cepharanthine (CEP), in a cell culture model of transforming growth factor-β1 (TGF-β1) and bleomycin (BLM)-induced pulmonary fibrosis rat models both in vitro and in vivo.

Rapid metagenomic identification of two major swine pathogens with real-time nanopore sequencing.

Metagenomic next-generation sequencing (mNGS) is a rapid deep-sequencing diagnostic tool for the unbiased identification of pathogens. In this study, we established a nanopore-sequencing-based mNGS protocol to detect two major viral pathogens of swine, Porcine reproductive and respiratory syndrome virus (PRRSV) and Porcine epidemic diarrhea virus (PEDV). Samples were spiked with the serially diluted viruses as standard references to define the specific protocols.

The deubiquitinase OTUD1 inhibits colonic inflammation by suppressing RIPK1-mediated NF-κB signaling.

The E3 ubiquitin ligase (E3)-mediated ubiquitination and deubiquitinase (DUB)-mediated deubiquitination processes are closely associated with the occurrence and development of colonic inflammation. Ovarian tumor deubiquitinase 1 (OTUD1) is involved in immunoregulatory functions linked to infectious diseases. However, the effect of OTUD1 on intestinal immune responses during colonic inflammatory disorders such as inflammatory bowel disease (IBD) remains unclear.

Polymorphism, expression and structure analysis of key genes in the ovarian steroidogenesis pathway in sheep (Ovis aries).

Background: Litter size is an important factor that significantly affects the development of the sheep industry. Our previous TMT proteomics analysis found that three key proteins in the ovarian steroidogenesis pathway, STAR, HSD3B1, and CYP11A1, may affect the litter size trait of Small Tail Han sheep.

Objective: The purpose of this study was to better understand the relationship between polymorphisms of these three genes and litter size.

Notch Signaling Activation in Cervical Cancer Cells Induces Cell Growth Arrest with the Involvement of the Nuclear Receptor NR4A2.

Cervical cancer is a second leading cancer death in women world-wide, with most cases in less developed countries. Notch signaling is highly conserved with its involvement in many cancers. In the present study, we established stable cervical cell lines with Notch activation and inactivation and found that Notch activation played a suppressive role in cervical cancer cells.

The complete mitochondrial genome of the hybrid [ (♀) × (♂)].

The complete mitochondrial genome of the hybrid of (♀) (♂) was characterized first in this study. The total length of the mitochondrial genome of was identical to the female parent as 16,581 bp, and the overall base composition was 31.87% A, 24.

The complete mitochondrial genome of the hybrid (♀)× (♂)].

The complete mitochondrial genome of the hybrid of (♀)×  (♂) was characterized first in this study. The total length of the genome was identical to the female parent as 16,581 bp, and the overall base composition was 31.80% A, 24.

Valproic acid exhibits different cell growth arrest effect in three HPV-positive/negative cervical cancer cells and possibly via inducing Notch1 cleavage and E6 downregulation.

We investigated the effect of valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, and the mechanism of VPA-induced growth inhibition on three cervical cancer cell lines with different molecular and genetic background. We found that VPA induced proliferation suppression, cell apoptosis and cell cycle arrest in all tested cell lines, with an increase of Notch1 active form ICN1 as a tumor suppressor and its target gene HES1. Noteworthy, blocking of Notch signaling with DAPT resulted in growth inhibition in ICN1-overexpressing CaSki and HT-3 cells.

Study on the correlation between extracellular matrix protein-1 and the growth, metastasis and angiogenesis of laryngeal carcinoma.

Objective: To investigate the correlation between extracellular matrix protein-1 (ECM1) and the growth, metastasis and angiogenesis of laryngeal carcinoma.

Materials And Methods: Forty-five samples with laryngeal benign and malignant tumors confirmed by pathology in Laiwu City People's Hospital from March 2006 to March 2011 were collected, in which there were 29 cases with laryngeal carcinoma and 16 with benign tumors. The expression of ECM1 and factor VIII-related antigens in patients with laryngeal carcinoma and those with benign tumors was respectively detected using immunohistochemical method, and the correlation between ECM1 staining grade and microvessel density (MVD) was analyzed.

Resistance to reinfection in mice as a vaccine model for giardiasis.

Infection with Giardia is the most commonly diagnosed parasitic cause of diarrhea in the developed world, yet no vaccine exists for human use and a commercially available veterinary vaccine is of limited utility. We have used the adult C57BL/6 mouse model of infection with Giardia duodenalis to better understand immunity to secondary infections with this parasite. Mice were primed by infection with either the GS or WB strains of Giardia and treated with metronidazole on day 7-12 to eliminate the primary infections.

Transgenically mediated shRNAs targeting conserved regions of foot-and-mouth disease virus provide heritable resistance in porcine cell lines and suckling mice.

Foot-and-mouth disease virus (FMDV) is responsible for substantial economic losses in livestock breeding each year, and the development of new strategies is needed to overcome the limitations of existing vaccines and antiviral drugs. In this study, we evaluated the antiviral potential of transgenic porcine cells and suckling mice that simultaneously expressed two short-hairpin RNAs (shRNAs) targeting the conserved regions of the viral polymerase protein 3D and the non-structural protein 2B. First, two recombinant shRNA-expressing plasmids, PB-EN3D2B and PB-N3D2B, were constructed and the efficiency of the constructs for suppressing an artificial target was demonstrated in BHK-21 cells.

Valproic acid suppresses cervical cancer tumor progression possibly via activating Notch1 signaling and enhances receptor-targeted cancer chemotherapeutic via activating somatostatin receptor type II.

Purpose: We investigated the effects of the anti-epilepsy drug valproic acid (VPA) alone and in combination in treating cervical cancer.

Methods: VPA was investigated for its effects on cervical cancer Hela cell proliferation and tumor growth via in vitro and in vivo assays.

Results: VPA induce cell growth suppression and cell cycle arrest, with an increase of Notch1 that acts as a tumor suppressor and the change of other tumor-associated genes such as p21, p63 and PCNA.

Anti-HBV efficacy of combined siRNAs targeting viral gene and heat shock cognate 70.

Background: Hepatitis B virus (HBV) infection is a major health concern with more than two billion individuals currently infected worldwide. Because of the limited effectiveness of existing vaccines and drugs, development of novel antiviral strategies is urgently needed. Heat stress cognate 70 (Hsc70) is an ATP-binding protein of the heat stress protein 70 family.

Cloning and characterization of porcine interferon-δ-related genes identified by genomic database screening.

Interferon-δ (IFN-δ) belongs to type I IFN. It was first identified by Lefevre in 1993. The porcine interferon-δ (PoIFN-δ)-related sequences were reported in 2009.