Tungsten Species on Beta Zeolite with Abundant Silanol Nests for Efficient Olefin Metathesis.

Tungstate-based catalysts for olefin metathesis generally suffer from insufficient reaction rates, which require relatively high temperatures for the satisfied activities. This issue is mainly due to the shortage of active WO species related to intrinsic low adsorption and poor activation of olefin molecules. Herein, we found that the silanol nests in dealuminated Beta zeolite (DeAl-Beta) were favorable for high dispersion of tungsten species, forming active WO species on the zeolite, which was helpful for the adsorption and activation of olefin molecules, thus facilitating the generation of metallocycle intermediates.

Case Report: SARS-CoV-2-associated immune dysfunction manifesting as concurrent fulminant type 1 diabetes mellitus and subacute thyroiditis.

Objectives: The association between SARS-CoV-2 infection and endocrine emergencies (such as fulminant type 1 diabetes mellitus and subacute thyroiditis) has received increasing attention. However, concurrent manifestations of these two conditions within a short period of time after infection are exceedingly rare, and the underlying mechanisms and clinical management strategies remain unclear.

Case Presentation: A 45-year-old Chinese man developed sudden polydipsia, polyuria, and cervical pain on day 7, within 2 weeks of SARS-CoV-2 infection.

Association between dietary fiber intake and gallstones among American adults: a cross-sectional study.

Background: Limited research has been conducted on the effects of dietary fiber (DF) intake on gallstones. We aimed to investigate the actual DF intake in a nationally representative sample in the United States (US) and explore its association with gallstones.

Methods: We used data from the National Health and Nutrition Examination Survey database, including the 2017-2020 and 2021-2023 cycles.

RACK1 attenuates pancreatic tumorigenesis by suppressing acinar-to-ductal metaplasia through inflammatory signaling modulation.

Purpose: Pancreatic ductal adenocarcinoma (PDA) remains one of the most lethal malignancies with limited early diagnostic and therapeutic options. Although receptor for activated C kinase 1 (RACK1) is an evolutionarily conserved scaffold protein, its functional role and mechanistic involvement in PDA pathogenesis remain elusive.

Methods: Using multimodal approaches including: (1) genetically engineered mouse models of pancreatitis and carcinogenesis, (2) patient-derived PDA tissues with matched normal specimens, (3) primary acinar cell 3D cultures, and (4) orthogonal gain/loss-of-function assays in PDA cell lines, we systematically investigated RACK1's spatiotemporal expression patterns and functional impacts.

CRISPR/Cas13a triggered-DNA walker amplified SERS sensor for ultrasensitive detection of cancer-related exosomal miRNA.

Accurate quantification of cancer-related miRNA in exosomes offers a promising approach for early and effective cancer diagnosis. However, reliably detecting extremely low-abundance exosomal miRNAs in complex bodily fluids remains a significant challenge. Herein, a CRISPR/Cas13a triggered-DNA walker amplified SERS sensor has been proposed for detection of cancer cell-derived exosomal miRNA-106a.

bra-miR9569 Targets the Gene to Negatively Regulate H-ATPases, Affecting Pollen Fertility in Chinese Cabbage ( L. ssp. ).

Ogura cytoplasmic male sterility (CMS) in Chinese cabbage () is characterized by complete pollen abortion, wherein stamens fail to produce viable pollen while pistils retain normal fertility. This maternally inherited trait is valuable for hybrid breeding. This study employed integrated analysis of miRNA, transcriptome, and degradome sequencing data aligned to the Chinese cabbage reference genome to elucidate the molecular function of bra-miR9569 in Ogura CMS pollen fertility and explore its associated pathways.

The safety and feasibility of telerobotic cholecystectomy via a 5G network: a prospective controlled clinical trial.

Background: Since advances in the fifth-generation (5G) communication technology have effectively facilitated the development of robotic telesurgery, the popularity of telerobotic operations has increased. The purpose of this study was to evaluate the safety and feasibility of robotic telecholecystectomy with a 5G wireless network using a new domestic robotic surgical system.

Methods: This study is a prospective controlled clinical trial.

[Blended teaching reform practice in Protein Engineering and Enzyme Engineering].

Protein Engineering and Enzyme Engineering is a professional core course for life science-related majors in higher education, aiming to help students apply theoretical knowledge to engineering practice. The rapid development of biomanufacturing has placed new demands on the training of protein and enzyme engineers. However, due to the complexity and strong interdisciplinary nature of the course contents, traditional offline teaching modes have poor teaching performance and are unable to meet the era's demand for high-tech innovative talents in the field of biomanufacturing.

3D printable phase change information storage label films for dynamic and multi-level anti-counterfeiting.

Anti-counterfeiting technology demands continuous innovation to address escalating global counterfeiting challenges. This study introduces 3D printable phase change information storage label films for dynamic, multi-level anti-counterfeiting applications. Utilizing extrusion-based 3D printing, customizable anti-counterfeiting labels with complex encrypted information such as barcodes and QR codes were fabricated.

Photosynthetic Electron Transport in Winter Wheat: Responses to Low-Temperature and Weak-Light Condition.

Spring low temperatures are a serious natural threat to wheat production in the Huang-Huai wheat region, and they are often accompanied by weak light environments during the day. To elucidate the response patterns and adaptation mechanisms of winter wheat leaves to low-temperature and weak-light environments, we simultaneously measured prompt chlorophyll a fluorescence, delayed chlorophyll a fluorescence, and modulated 820 nm light reflection; moreover, we analyzed the effects of low temperature and weak light treatment for different duration (2 h and 4 h) on the donor-side activity of photosystem II (PSII), the degree of PSII unit dissociation, the efficiency of light energy absorption and capture by PSII, electron transfer to Q and PSI terminal, PSI activity and cyclic electron transport activity in isolated wheat leaves under controlled conditions. The results, which were corroborated using the three methods, revealed that in low-temperature and weak-light environments, the degree of PSII unit dissociation, and the efficiency of light energy absorption, capture, and electron transfer to Q decreased, while the donor-side activity remained unaffected.

Effects of different leaf colors on the quality of hawk black tea: sensory evaluation and metabolomics.

Leaf color, a vital phenotypic trait, influences the flavor quality of tea. This study investigated the flavor and metabolite profiles of hawk black tea (HBT) processed from leaves of different colors. Sensory evaluation revealed that purple-leaf HBT (PHBT) exhibited prominent floral-fruity notes, green-leaf HBT (GHBT) showed fresh camphoraceous characteristics, while mixed-leaf HBT (MHBT) demonstrated optimal flavor with the highest overall acceptability.

Characterization of key astringent compounds and optimization of the fixation process of early tea in Chuanyu region.

Due to inadequate fixation, early tea produced in the Chuanyu region exhibits high astringency. To mitigate the astringency of early tea, this study identified the astringent compounds present in the tea, subsequently optimized its fixation process based on the key astringent substances. Initially, 20 early tea samples were categorized into three groups (low, middle, and high astringency) according to the differences in astringency.

Synergistic integration of light signal, hormone dynamics, and carbohydrate metabolism orchestrates rhizome bud development in Arundo donax.

The rhizome bud development is governed by light signal, hormonal dynamics, carbohydrate metabolism, and cell cycle regulation in Arundo donax. Rhizomatous plants reproduce asexually through transplantation of rhizome segments bearing buds, which develop into new individuals. However, the regulatory mechanism governing rhizome bud differentiation and development is still unknown.

Co-infection of with Epstein-Barr virus in gastric organoids enhances cell proliferation and morphogenesis.

Unlabelled: () is a key cause of chronic inflammation and gastric cancer, and Epstein-Barr virus (EBV) also contributes to gastric tumorigenesis. However, the role of EBV co-infection in -related cancers remains unclear because of the lack of efficient models. Organoids, which are derived from self-renewing stem cells, closely mimic tissue structure and function, making them ideal for disease modeling.

-derived nCEV vesicles promote cutaneous wound healing and inhibit HPV16 infection.

Unlabelled: Cervical cancer (CC) is a common gynecological malignancy, primarily associated with persistent infection by high-risk human papillomavirus (HPV). The vaginal microbiota, dominated by species, plays a critical role in maintaining a healthy vaginocervical microenvironment. However, the influence of on the pathogenesis and treatment of CC remains underexplored.

Establishment of a YKL-40 time-resolved Immunoassay and Application in Diabetic Kidney Disease.

To develop a highly sensitive, wide-linear-range time-resolved fluorescence immunoassay (TRFIA) to detect serum YKL-40 and to measure its concentrations in patients with diabetic kidney disease (DKD). A double-antibody sandwich method was employed to establish the YKL-40 TRFIA, which utilizes two antibodies targeting distinct epitopes on the YKL-40 antigen (a coating antibody and a labeled antibody). The assay's linearity, sensitivity, accuracy, specificity, and recovery rate were evaluated.

and Genes Are Involved in SlMYB15-Regulated Redox Homeostasis to Enhance Cold Tolerance in Tomato.

Cold stress is a major abiotic factor limiting plant growth and productivity. MYB transcription factors are important regulators of stress responses. Our previous study identified SlMYB15 as a positive regulator of cold tolerance in tomato.

Integration of Virtual Simulation Software in Preclinical Teaching in Temporomandibular Joint Surgery: Improving Learning Outcomes.

Objectives: The integration of virtual simulation software in medical education has revolutionized the way of students' learning. This study evaluated the impact of a virtual simulation software in pre-clinical teaching of temporomandibular joint surgery.

Methods: A total of 54 fourth-year stomatology undergraduates were randomly categorized into two groups after a pre-test: one subjected to traditional teaching and the other to virtual simulation teaching.

Cisplatin-Based Combinations-Associated Vasculopathy - A Disproportionality Analysis of Real-World Pharmacovigilance Data.

Introduction: Cisplatin, a platinum-based antineoplastic agent belonging to the alkylating class, is one of the most widely used chemotherapeutic agents in the treatment of solid tumors and hematologic malignancies. Cisplatin works by forming covalent bonds in DNA, resulting in cell cycle arrest, inadequate repair, and ultimately, apoptotic or non-apoptotic cell death. Despite its efficacy, cisplatin is known to be highly toxic, showing nephrological, Gastrointestinal (GI), and hepatotoxicity, but there is limited data on its association with adverse vascular events.

Polarization-entangled photon pairs generation from a single lithium niobate waveguide with single poling period.

Polarization-entangled photon pairs are essential sources for photonic quantum information processing. However, generating entangled photon pairs with large detuning via spontaneous parametric down-conversion (SPDC) often requires complex configurations to compensate for phase matching. Here, we propose a simple and efficient scheme to generate polarization-entangled photon pairs based on type-0 SPDC in a thin-film lithium niobate waveguide with a single poling period.

2-aminoethoxydiphenylborane intervenes intracellular calcium signaling and attenuates myocardial ischemia-reperfusion injury in mice through ITPR1/MCU pathway.

Myocardial ischemia-reperfusion (MI/R) injury frequently occurs during the clinical management of ischemic heart disease. The underlying mechanism includes neutrophil infiltration, heightened intracellular Ca levels, mitochondrial energy metabolism disorder. This study investigated the pathological role of the inositol 1,4,5-trisphosphate receptor/mitochondrial calcium uniporter (ITPR1/MCU) pathway in regulating disturbances in intracellular calcium ([Ca]) and mitochondrial calcium ([Ca]) levels during MI/R injury.

Flexible porous carbon fiber composite electrodes for hydrogen and chlorine production with antibacterial applications.

Flexible porous carbon fiber composite electrodes (Pt@C-PES cathode/RuO@C-PES anode) for hydrogen and chlorine evolution reactions (HER/CER) in acidic NaCl electrolyte (1 M HCl + 5 M NaCl) were developed in this work. Functionalized with Pt and RuO electrodeposition and drop-coating, the electrodes demonstrated low overpotentials of 71 mV@25 mA cm (HER) and 77 mV@25 mA cm (CER). When applied in a two-electrode H-cell system, faradaic efficiencies of 99.

FAM111B knockdown attenuates tumorigenesis of ovarian cancer via the downregulation of MYC.

Background: Ovarian cancer, a prevalent malignancy with the highest fatality rate among gynecological cancers, continues to face challenges in the development of effectively targeted therapeutic approaches. While the FAM111B gene has been implicated in various cancer types, its specific role in ovarian cancer remains poorly understood.

Methods: The ES2 and A2780 ovarian cell lines were exploited to explore the cellular proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in FAM111B knockdown experiments.

Peptidomimetics based on natural cinnamic acid scaffold: Synthesis, bio-evaluation and structure-activity relationships.

A total of twenty-one peptidomimetics based on cinnamic acid scaffold were synthesized through structure-activity relationship driven (SAR-driven) optimization of natural molecule (CSDK450160). Systematic bio-evaluation against twelve phytopathogenic fungi for these compounds revealed structure-dependent antifungal specificity, with cinnamic acid-peptide conjugates demonstrating pronounced inhibitory efficacy against Botrytis cinerea and Sclerotinia sclerotiorum. In planta antifungal assays showed that compound I-1 exhibited superior inhibitory potency against S.

Cooperative CCL2/CCR2 and HGF/MET signaling enhances breast cancer growth and invasion associated with metabolic reprogramming.

With over 60,000 cases diagnosed in women annually, ductal carcinoma in situ (DCIS) is the most common form of pre-invasive breast cancer in the US. Despite standardized therapy, under-treatment and over-treatment are prevailing concerns. By understanding the mechanisms regulating DCIS progression, we may develop tailored strategies to improve treatment.