Mid-Term Management of Operating Room Equipment: Improving Surgical Quality.

This study focuses on the mid-term management theory of equipment lifecycle and systematically examines the impact of operating room instrument management on operational efficiency optimization. The review mainly covers four key aspects: operator training, information technology application, cost control, and quality assurance. The results indicate that mid-term management plays a crucial role in the full lifecycle management of operating room equipment and plays a key role in improving the quality of operating room operations.

and Genes Are Involved in SlMYB15-Regulated Redox Homeostasis to Enhance Cold Tolerance in Tomato.

Cold stress is a major abiotic factor limiting plant growth and productivity. MYB transcription factors are important regulators of stress responses. Our previous study identified SlMYB15 as a positive regulator of cold tolerance in tomato.

CircNOX4 promotes proliferation and resistance by regulating the hsa-miR-6884-5p/YWHAG Axis and ERK signaling pathway in cholangiocarcinoma.

The biological function of circular RNAs (circRNAs) has been increasingly implicated in tumor drug resistance. However, their role in intrahepatic cholangiocarcinoma (ICC) remains poorly understood, and the underlying mechanisms are largely unexplored. In this study, we identified a key circRNA, circNOX4, through data mining and experimental validation in ICC.

Impacts of Early Weaning on Lamb Gut Health and Immune Function: Short-Term and Long-Term Effects.

Despite the known impacts of weaning on animal health, the underlying molecular mechanisms remain unclear, particularly how psychological and nutritional stress differentially affect gut health and immune function over time. This study hypothesized that early weaning exerts distinct short- and long-term effects on lamb stress physiology, immunity, and gut health, mediated by specific molecular pathways. Twelve pairs of full-sibling male Hu sheep lambs were assigned to control (CON) or early-weaned (EW) groups.

Dietary baking soda (NaHCO) therapy recovered urolithiasis-induced kidney injury in mice by inhibition of oxidative stress, pyroptosis, and inflammation through gut-kidney axis.

Background: Urolithiasis is one of the most common urological diseases, and its incidence has increased globally in recent years. Multiple potential mechanisms are involved in urolithiasis, including renal oxidative stress, inflammatory response, fibrosis, pyroptosis, and gut microbiota disturbance. Sodium bicarbonate (NaHCO) is commonly used clinically to alkalize urine and slow the progression of chronic kidney disease, including urolithiasis.

Bile acids activate cancer-associated fibroblasts and induce an immunosuppressive microenvironment in cholangiocarcinoma.

Cholangiocarcinoma (CCA) is a highly lethal malignancy originating from the biliary tract and characterized with exposure to high levels of bile acids (BAs). Immunotherapies have demonstrated limited efficacy in CCA, but the underlying mechanism remains elusive. In this study, we reveal that the excessive BAs specifically activate GPBAR1 on cancer-associated fibroblasts (CAFs) to express high levels of CXCL10, enhancing epithelial-mesenchymal transition (EMT) and metastasis of CCA cells and creating an immunosuppressive tumor microenvironment (TME) by recruiting neutrophils in CCA.

Development and validation of a nomogram combining cytokines with traditional clinical parameters in predicting the risk of postoperative sepsis after ureteroscopic lithotripsy.

Background: Sepsis is one of the most severe complications of ureteroscopic lithotripsy (URSL) surgery and is the main cause of death in hospitalized patients. Traditional preoperative patient management and risk assessment systems need to be updated urgently to reduce the occurrence of postoperative sepsis. This study aimed to construct a novel nomogram to preoperatively predict the risk of septic complications after URSL.

The role of gut microbiota in Tirzepatide-mediated alleviation of high-fat diet-induced obesity.

Purpose: In this study, we aim to explore the effects of tirzepatide on the gut microbiota in mice with obesity induced by a high-fat diet.

Methods: Forty male C57BL/6J mice, aged six weeks, were randomly assigned to one of four experimental groups: normal control diet, normal control diet with tirzepatide treatment (NCD + TZP), high-fat diet and high-fat diet with tirzepatide treatment (HFD + TZP). Mice in the HFD group were fed a high-fat diet for ten weeks to establish an obesity model.

Unveiling the Key Role of Surface Oxidation through Quantifying Reaction Kinetics in Heterogeneous Catalysis for Water Treatment.

Quantifying surface-specific kinetics of organic oxidation in heterogeneous catalytic systems remains a critical challenge due to the interplay of adsorption and complex reaction mechanisms. In this study, we introduce a novel kinetic framework that distinguishes surface reaction kinetics () from conventional solution-phase kinetics (), using nitrogen-doped porous carbon (NPC) as a model catalyst with high adsorption capacity and exceptional efficacy in peroxymonosulfate (PMS) activation. By directly analyzing the selective oxidation of fully adsorbed -substituted phenolic compounds (-PCs), we precisely quantified and established robust QSAR models with remarkable linear correlations ( = 0.

Impact of crumbled pelleted starter feed and alfalfa inclusion on feed intake, growth, and rumen microbiota in young lambs.

Pelleted feed has multiple advantages in animal production, but its hardness may limit the intake of young lambs whose teeth and digestive systems are not yet fully developed, especially when the feed contains alfalfa and is produced with a high compression ratio in small diameters. This study aimed to evaluate the effects of removing alfalfa from starter feed or post-pelleting crumbling on lamb performance. We hypothesized that crumbling pelleted feed could reduce hardness, thereby increasing intake and enhancing performance.

2bRAD-M Reveals the Characteristics of Urinary Microbiota in Overweight Patients with Urinary Tract Stones.

Urinary tract stone (UTS) is a common disease significantly impacting human health. Obesity influences stone formation and increases UTS incidence, yet the differences in the urinary microbiota and pathways between overweight and healthy-weight UTS patients remain unclear. In this study, 16 patients were analyzed: 8 overweight and 8 healthy-weight UTS patients.

The role of PSMC4 in non-small cell lung cancer: implications for prognosis, diagnosis, and immune microenvironment modulation.

Introduction: Non-small cell lung cancer (NSCLC) remains a principal cause of cancer-related mortality. The discovery of novel biomarkers is pivotal for enhancing early diagnosis, refining prognostic evaluations, and optimizing targeted therapeutic strategies. Proteasome 26S subunit ATPase 4 (PSMC4), a proteasome subunit essential for protein degradation, influences tumor progression regulatory mechanisms.

Purification, identification, and synthesis of novel antioxidant peptides from peanut meal and their protective effects against H₂O₂-induced oxidative damage in HepG2 cells.

Peanut meal, a by-product of peanut oil extraction, is rich in protein but remains underutilized. Previous studies have indicated that peanut protein hydrolysate (PPH) exhibits strong antioxidant activity, but the antioxidant mechanisms and functional effects of PPH remain unclear. Therefore, this study aimed to purify, identify, and synthesize novel antioxidant peptides from PPH and evaluate their protective effects against oxidative damage in HepG2 cells.

Microbial insights into ruminal fiber degradation and feed efficiency of Hu sheep.

Ruminal fiber degradation is essential for feed conversion efficiency in sheep; however, it remains unclear whether individual variations in ruminal fiber degradation directly affect feed conversion efficiency. Here, the relationship between ruminal fiber degradation rate and feed conversion efficiency and influence of rumen structure, function, and microbiota on fiber degradation were investigated. A total of 190 male Hu lambs were randomly selected, raised from birth to 180 days, and slaughtered.

Characterization of the gut microbiota in urinary calculi patients with preoperative urinary tract infection.

Background: Urinary tract infection is one of the most common comorbidities of urinary stones. Disorders of gut microbiota can affect various infectious diseases and the formation of the stones. Therefore, alterations in the gut bacteria profile may be a potential risk factor for the development of infections in patients with urinary tract stones.

Patterns and outcomes of early and late recurrence after hepatectomy for hepatocellular carcinoma with microvascular invasion: a multicenter study in China.

Background: A few studies focus on the long-term outcomes and surveillance strategies for patients with hepatocellular carcinoma (HCC) and microvascular invasion (MVI) who experience postoperative recurrence. The aim of this study was to explore the patterns and prognosis of early and late recurrence (ER and LR) after hepatectomy of such patients.

Methods: Consecutive patients with HCC and MVI after hepatectomy from 26 centers in China from 2009 to 2020 were included.

p23 protects against ferroptosis of brain microvascular endothelial cells in ischemic stroke.

Ferroptosis is a type of iron‑dependent regulated cell death that differs from apoptosis, autophagy or necrosis. p23 serves as a co‑chaperone and performs a unique biological function in various diseases by binding to client proteins to modulate their biological functions; however, its effect on ferroptosis remains largely unknown. In the present study, the effects of cerebral ischemia/reperfusion (I/R) injury (CIRI) or oxygen‑glucose deprivation/reoxygenation on the blood‑brain barrier (BBB) and ferroptosis in brain microvascular endothelial cells (BMECs), as well as the expression of p23, were examined.

Multifaceted roles of ninjurin1 in immunity, cell death, and disease.

Ninjurin1 (NINJ1) is initially identified as a nerve injury-induced adhesion molecule that facilitates axon growth. It is initially characterized to promote nerve regeneration and mediate the transendothelial transport of monocytes/macrophages associated with neuroinflammation. Recent evidence indicates that NINJ1 mediates plasma membrane rupture (PMR) in lytic cell death.

Perillaldehyde ameliorates sepsis-associated acute kidney injury via inhibiting HSP90AA1-mediated ferroptosis and pyroptosis: Molecular structure and protein interaction of HSP90AA1.

Heat shock protein 90α (HSP90AA1) is a molecular chaperone involved in a variety of cellular processes. Special attention is paid to how perillaldehyde ameliorates kidney injury by inhibiting HSP90AA1-mediated iron and pyrotoxicity, and in-depth analysis of the molecular structure and protein interactions of HSP90AA-1. The interaction between perillaldehyde and HSP90AA1 and the effect of perillaldehyde on the molecular structure of HSP90AA1 were analyzed by molecular docking and surface plasmon resonance technique.

GASTRODIN PROTECTS AGAINST SEPSIS-ASSOCIATED ENCEPHALOPATHY BY SUPPRESSING FERROPTOSIS.

Background: Sepsis-associated encephalopathy (SAE) represents a severe complication of sepsis, substantially elevating both mortality and healthcare costs for patients. Gastrodin (GAS), a principal bioactive constituent of Gastrodia elata Blume, is neuroprotective in various neurological disorders, including ischemic stroke, epilepsy, Alzheimer's disease, and neuropathic pain. In this study, we sought to investigate whether GAS could serve as a protective agent against SAE.

Protocol of REACH-01: a single-arm, open label, prospective study of HAIC sequential TAE combined with tislelizumab and surufatinib in unresectable intrahepatic cholangiocarcinoma.

Introduction: Gemcitabine and cisplatin remain the cornerstone for the treatment of advanced or unresectable biliary tract cancers, but the incidence rate of the grade 3 or 4 toxic effects is high (70.7%). In recent years, significant progress has been achieved in the systemic treatment of cholangiocarcinoma with immune checkpoint inhibitors (ICIs), targeted therapy, and hepatic artery infusion chemotherapy (HAIC).