First-line benmelstobart plus anlotinib versus sunitinib in advanced renal cell carcinoma (ETER100): a multicentre, randomised, open-label, phase 3 trial.

Background: The underexplored potential of PD-L1 blockade in advanced renal cell carcinoma highlights an urgent need for novel agents. This trial aimed to compare benmelstobart (a novel PD-L1 inhibitor) plus anlotinib with sunitinib as first-line treatment for advanced renal cell carcinoma.

Methods: ETER100 was a multicentre, randomised, open-label, phase 3 trial conducted at 37 medical sites in China.

Physical and chemical niche of human growth plate for polarized bone development.

Growth plate (GP), a critical cartilaginous structure in amniotes, underpins longitudinal bone growth, yet the intricate mechanisms behind its polarized mineralization during evolution remain unclear. Herein, employing high-resolution analytical techniques, we reveal that the GP-epiphysis interface displays a sharp transition in tissue modulus, acting as a protective shell for the underlying GP, whereas the GP-metaphysis interface exhibits a gradual modulus increase, enabling efficient load redistribution to the metaphysis. This mechanical microenvironment contributes to unique microstructural and compositional transformations from GP to epiphysis and metaphysis.

From niche to organoid: Engineering bone tissues through microenvironmental insights.

The construction of bone organoids represents a transformative approach in tissue engineering, offering unprecedented opportunities for studying bone biology, disease modeling, and regenerative medicine. The intricate understanding of the skeletal microenvironment, or niche, which governs cellular behavior, tissue organization, and functional maturation, is critical important to construct bone organoid. This review explored insights into the skeletal microenvironment, including the roles of extracellular matrix components, mechanical cues, biochemical signaling, and cellular interactions.

Characteristic metabolite profile of 10 colorectal cancer-related bacteria.

Background: Tumor metabolomics of colorectal cancer (CRC) is significantly different from normal tissues, due to nutrient deprivation, metabolite accumulation, acidity and hypoxia. Besides, gut microbiota has been confirmed to affect the progression of CRC. Microbiota metabolites might participate in the metabolic reprogramming of CRC cells and further regulate tumor microenvironment.

Correction: Gut microbiota, metabolites, and cytokines in relation to the risk of prostate cancer in the Asian population.

[This corrects the article DOI: 10.3389/fonc.2024.

CENPF overexpression in bladder cancer cells enhances proliferation, migration, invasion, and apoptosis.

Bladder cancer has been considered as one of the most common urinary malignancies. Growing evidence has indicated that Centromere Protein F (CENPF) is a promising molecular biomarker for many human malignant tumors. However, the role of CENPF in bladder cancer (BC) proliferation, migration, invasion, cell cycle and apoptosis thereof remain unclear.

Dietary baking soda (NaHCO) therapy recovered urolithiasis-induced kidney injury in mice by inhibition of oxidative stress, pyroptosis, and inflammation through gut-kidney axis.

Background: Urolithiasis is one of the most common urological diseases, and its incidence has increased globally in recent years. Multiple potential mechanisms are involved in urolithiasis, including renal oxidative stress, inflammatory response, fibrosis, pyroptosis, and gut microbiota disturbance. Sodium bicarbonate (NaHCO) is commonly used clinically to alkalize urine and slow the progression of chronic kidney disease, including urolithiasis.

Development and validation of a nomogram combining cytokines with traditional clinical parameters in predicting the risk of postoperative sepsis after ureteroscopic lithotripsy.

Background: Sepsis is one of the most severe complications of ureteroscopic lithotripsy (URSL) surgery and is the main cause of death in hospitalized patients. Traditional preoperative patient management and risk assessment systems need to be updated urgently to reduce the occurrence of postoperative sepsis. This study aimed to construct a novel nomogram to preoperatively predict the risk of septic complications after URSL.

Comprehensive multi-omics analysis of nucleotide metabolism: elucidating the role and prognostic significance of UCK2 in bladder cancer.

Nucleotide metabolism reprogramming is a hallmark of cancer, yet its systematic investigation remains limited. Here, we performed a comprehensive multi-omics analysis of key nucleotide metabolism genes across various cancer types using TCGA, GTEx, and other public datasets. Uridine-cytidine kinase 2 (UCK2), a key enzyme in the pyrimidine salvage pathway, was identified as consistently upregulated and genomically amplified, particularly in bladder cancer (BLCA).

ADT increases prostate cancer cell invasion via altering AR/SALL4/SOX2-OCT4 stem cell signaling.

Early studies indicated that the androgen-deprivation-therapy with antiandrogen Enzalutamide (Enz) could increase prostate cancer patients' survival by an average of 4.8 months. Yet Enz might also have some adverse effects via increasing the prostate cancer (PCa) cell invasion.

Nano-delivery of miRNA inhibiting CENPF combined with cisplatin for bladder cancer treatment.

Bladder cancer (BCa) presents a substantial global health burden, with high rates of recurrence and metastasis that limit the effectiveness of current therapies. New therapeutic strategies are urgently needed. This study introduces a novel nanotherapeutic approach utilizing polydopamine (PDA) nanoparticles to co-deliver cisplatin and miR-205-5p for BCa treatment.

2bRAD-M Reveals the Characteristics of Urinary Microbiota in Overweight Patients with Urinary Tract Stones.

Urinary tract stone (UTS) is a common disease significantly impacting human health. Obesity influences stone formation and increases UTS incidence, yet the differences in the urinary microbiota and pathways between overweight and healthy-weight UTS patients remain unclear. In this study, 16 patients were analyzed: 8 overweight and 8 healthy-weight UTS patients.

Immunosuppressive role of benzo[a]pyrene exposure in prostate cancer progression.

Epidemiological studies indicate that prostate cancer (PCa) is the second prevalent malignant tumor affecting men globally. Environmental pollution such as cigarette smoke is one of the important risk factors for the development of prostate cancer. However, as one of the main carcinogens in cigarette smoke, the role of benzo[a]pyrene (BaP) in prostate cancer is still unclear.

Management of a nephrostomy tube misplacement into the inferior vena cava following PCNL.

A 50-year-old female with left flank pain and 3.1 cm renal calculi underwent PCNL. Intraoperatively, an anatomical variant led to accidental insertion of a nephrostomy tube into the inferior vena cava (IVC), while a second tube was correctly placed.

Oncogenic and immunological functions of USP35 in pan-cancer and its potential as a biomarker in kidney clear cell carcinoma.

Background: Ubiquitin-specific protease 35 (USP35) has gained attention as a regulator in cancer progression. However, its specific role in kidney clear cell carcinoma (KIRC) remains unclear.

Methods: USP35 expression in KIRC tumor and normal tissues was evaluated using TCGA data.

3D-Printed Dual-Lineage Inductive Approach for Efficient Osteochondral Regeneration.

Osteochondral defect regeneration is challenging due to the mismatch between cartilage and subchondral bone. We developed a functionalized scaffold replicating the natural architecture, biochemical and biomechanical environment of both tissues to promote concurrent regeneration. Our bilayered, zone-specific scaffold combines tailored materials for each tissue type: gelatin methacryloyl (GelMA), modified hyaluronic acid, and umbilical cord-derived extracellular matrix (ECM) for the cartilage layer; GelMA, placenta-derived ECM, and nano amorphous calcium phosphate for the osseous layer.

Organelle-tuning condition robustly fabricates energetic mitochondria for cartilage regeneration.

Mitochondria are vital organelles whose impairment leads to numerous metabolic disorders. Mitochondrial transplantation serves as a promising clinical therapy. However, its widespread application is hindered by the limited availability of healthy mitochondria, with the dose required reaching up to 10 mitochondria per injection/patient.

Living joint prosthesis with in-situ tissue engineering for real-time and long-term osteoarticular reconstruction.

The reconstruction of large osteoarticular defects caused by tumor resection or severe trauma remains a clinical challenge. Current metal prostheses exhibit a lack of osteo-chondrogenic functionality and demonstrate poor integration with host tissues. This often results in complications such as abnormal bone absorption and prosthetic loosening, which may necessitate secondary revisions.

An Integrated Approach Utilizing Single-Cell and Bulk RNA-Sequencing for the Identification of a Mitophagy-Associated Genes Signature: Implications for Prognostication and Therapeutic Stratification in Prostate Cancer.

: Prostate cancer, notably prostate adenocarcinoma (PARD), has high incidence and mortality rates. Although typically resistant to immunotherapy, recent studies have found immune targets for prostate cancer. Stratifying patients by molecular subtypes may identify those who could benefit from immunotherapy.

Combining transcriptomic and metabolomic insights to guide the clinical application of adipose- and bone marrow-derived mesenchymal stem cells.

Adipose-derived mesenchymal stem cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs) have shown great potential in clinical applications. However, the similarities and differences between these two cell types have not been fully elucidated. Recent advances in transcriptomic and metabolomic research have provided valuable insight into the characteristics and functions of ADSCs and BMSCs.

Perillaldehyde ameliorates sepsis-associated acute kidney injury via inhibiting HSP90AA1-mediated ferroptosis and pyroptosis: Molecular structure and protein interaction of HSP90AA1.

Heat shock protein 90α (HSP90AA1) is a molecular chaperone involved in a variety of cellular processes. Special attention is paid to how perillaldehyde ameliorates kidney injury by inhibiting HSP90AA1-mediated iron and pyrotoxicity, and in-depth analysis of the molecular structure and protein interactions of HSP90AA-1. The interaction between perillaldehyde and HSP90AA1 and the effect of perillaldehyde on the molecular structure of HSP90AA1 were analyzed by molecular docking and surface plasmon resonance technique.

Engineering cardiology with miniature hearts.

Cardiac organoids offer sophisticated 3D structures that emulate key aspects of human heart development and function. This review traces the evolution of cardiac organoid technology, from early stem cell differentiation protocols to advanced bioengineering approaches. We discuss the methodologies for creating cardiac organoids, including self-organization techniques, biomaterial-based scaffolds, 3D bioprinting, and organ-on-chip platforms, which have significantly enhanced the structural complexity and physiological relevance of cardiac models.

Gut microbiota, metabolites, and cytokines in relation to the risk of prostate cancer in the Asian population.

Purpose: Studies have shown that gut microbiota is involved in the tumorigenesis and development of prostate cancer. We aimed to perform a comprehensive analysis of causal associations of gut microbiota, metabolites, and cytokines with prostate cancer in the Asian population.

Patients And Methods: Genome-wide association study (GWAS) summary datasets were collected from the public databases.