Safety of Minimally Invasive Sublobar Pulmonary Metastasectomy in the Frail Patient.

Background And Objectives: Minimally invasive (MI) pulmonary metastasectomy may improve access in frail patients who are at high-risk for complications after thoracotomy. We compared postoperative complications among frail and non-frail patients undergoing pulmonary metastasectomy.

Methods: The Nationwide Readmissions Database (2017-2019) identified adult patients who underwent pulmonary metastasectomy.

Extraintestinal pathogenic Escherichia coli (ExPEC) O-serotype distribution and antibiotic resistance profiles among the aging population in China: A longitudinal, multi-center study.

Extraintestinal pathogenic Escherichia coli (ExPEC) is a leading cause of community-acquired bacteremia and sepsis, which contributes to the substantial burden of invasive E. coli disease (IED) in older adults. This study aimed to estimate the O-serotype distribution of blood and sterile site ExPEC among older adults in China and the characteristics of antimicrobial resistance, O-serotypes, and O genotypes.

Passive eDNA Sampling Characterizes Fish Community Assembly in the Lancang River of Yunnan, China.

This study aimed to determine the practical efficacy of passive eDNA samplers (PEDS) for monitoring fish diversity in riverine ecosystems. It investigated the utility of environmental DNA (eDNA) in accurately depicting fish composition and diversity within the Lancang River. Environmental DNA technology, particularly PEDS, may be used as a substitute for traditional water filtration techniques.

SPRY1 Interaction with CBL Promotes Vimentin Stabilization to Enhance Malignant Progression and Metastasis in Wilms Tumor.

Background: SPRY1 from the Sprouty family is frequently overexpressed in many tumors and is accompanied by poor prognosis and metastatic progression. However, its role in the tumorigenesis of Wilms tumor (WT) remains unknown. The aim of this study is to elucidate the functional role of SPRY1 in the oncogenesis of Wilms tumor.

The Effect of the Fresh Latex Ratio on the Composition and Properties of Bio-Coagulated Natural Rubber.

By proportionally blending fresh latex from PR107, Reyan 72059, and Reyan 73397, and employing both acid- and enzyme-assisted microbial coagulation methods, this study analyzed the effects of the specific latex formulation on the following: physicochemical properties, non-rubber components, molecular weight and distribution, vulcanization characteristics of compounded rubber, and physical-mechanical properties of vulcanized natural rubber. The results indicate that, compared to acid-coagulated natural rubber, enzyme-assisted microbial coagulated natural rubber exhibits slightly lower levels of volatile matter, impurities, plasticity retention index (PRI), nitrogen content, calcium ions (Ca), iron ions (Fe), and fatty acid content. Conversely, it demonstrates higher values in ash content, initial plasticity (P), Mooney viscosity (M), acetone extract, magnesium ions (Mg), copper ions (Cu), manganese ions (Mn), gel content, molecular weight and distribution, and glass transition temperature (Tg).

A calcium manganese-based pancatalytic nanozyme as a cell pyroptosis inhibitor for efficient inflammatory bowel disease treatment.

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder marked by dysregulated immune responses and pyroptosis of intestinal epithelial cells, a type of programmed cell death that aggravates inflammation. Current therapeutic strategies frequently encounter limitations in both efficacy and specificity. Herein, we developed a calcium manganese-based (CaMnO) pancatalytic nanozyme designed to mitigate pyroptosis and alleviate IBD symptoms.

Hsa_circ_0005571 promotes the proliferation and invasion of colorectal cancer cells.

Dysregulated expression of circular RNAs (circRNAs) has been implicated in the initiation and progression of various diseases, including cancer. In this study, we investigated the role of hsa_circ_0005571 in modulating the biological characteristics of colorectal cancer (CRC) cells. Initially, the circRNA expression profiles of CRC tissues and corresponding normal tissues were analyzed using bioinformatics to identify differentially expressed circRNAs.

Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment.

Dendritic cell (DC)-based vaccines for solid tumors face major challenges, including limited tumor-specific antigens and immunosuppressive stroma. Here, we present a therapeutic nanovaccine (UCNP@MOF@MI@FM [UMMF]) composed of a DC/tumor fused cytomembrane-coated UCNP@MOF nanoparticle, co-loaded with a MutT homolog 1 (MTH1) inhibitor and combined with tetrahydrobiopterin (BH4). The fused membrane facilitates dual targeting to tumors and lymph nodes while enabling broad-spectrum tumor antigen presentation.

The prediction models for the optimal timing of surgical intervention for necrotizing enterocolitis: nomogram vs. five machine learning models.

Background: Necrotizing enterocolitis (NEC) is one of the most common diseases that pose serious threats to the life of newborns. In clinical practice, NEC is typically treated by surgical intervention, but it is still difficult to identify the timing of surgical intervention for this disease. Therefore, this study was conducted to establish a machine learning (ML) model for identifying the optimal timing of surgical intervention for NEC by comparing logistic regression (LR) models with ML models and to visualize important influencing indicators via a nomogram.

Genomic and Immune Landscape of Pancreatic Ductal Adenocarcinoma Associated with Germline Pathogenic Variants in ATM.

Background: Germline pathogenic variants (PVs) in ATM increase the risk of pancreatic ductal adenocarcinoma (PDAC) but the underlying tumor biology of PDAC associated with germline PVs in ATM have not been adequately explored.

Experimental Design: Whole-genome (WGS), whole-exome (WES), and RNA-sequencing were performed on PDAC tumors from 25 germline ATM PVs carriers diagnosed at Mayo Clinic between 2007 and 2017. Somatic and copy number alterations, mutational signatures, transcriptomic subtypes, and the immune landscape were evaluated.

[The impact of different chest compression frequencies on cardiopulmonary resuscitation outcomes in domestic pigs].

Objective: To compare the effects of different chest compression rates (60-140 times/min) on hemodynamic parameters, return of spontaneous circulation (ROSC), resuscitation success, and survival in a porcine model of cardiac arrest (CA) followed by cardiopulmonary resuscitation (CPR).

Methods: Forty healthy male domestic pigs were randomly divided into five groups based on chest compression rate: 60, 80, 100, 120, and 140 times/min (n = 8). All animals underwent standard anesthesia and tracheal intubation.

Deproteinization and controlled starch prehydrolysis in intact pulse cotyledon cells regulates starch retrogradation and digestion.

Intact pulse cotyledon cells are known for their intact cellular structure and dense protein matrix, leading to slower starch digestibility. However, the retrogradation and digestibility of retrograded intracellular starch has rarely been investigated, and our preliminary results indicated the difficulty of intracellular starch retrogradation. To test mechanistic principles of intracellular starch retrogradation, intact pulse cells were extracted and treated with protease followed by pancreatic α-amylase (0-20 min) and pullulanase (24 h) prehydrolysis, and then controlled retrogradation (4 °C, 5 d).

Evolutionary Conservation and Immunoregulatory Function of LOXL3 Gene in Lamprey.

Lysyl oxidase (LOX) family enzymes play a pivotal role in extracellular matrix (ECM) remodeling and tissue homeostasis, and have evolved diverse functions in vertebrate immunity and metabolism. However, the evolutionary trajectory of these multifunctional roles-particularly in jawless vertebrates-remains obscure. Here, we investigate LOXL3 in the lamprey (Lethenteron reissneri), a jawless vertebrate that retains ancestral features of early vertebrate evolution.

Combined Flt3L and CD40 agonism restores dendritic cell-driven T cell immunity in pancreatic cancer.

T cell-directed immunotherapies have largely failed to slow progression of pancreatic ductal adenocarcinoma (PDAC) because of poor tumor antigenicity and an immunosuppressive tumor microenvironment. We hypothesized that conventional dendritic cell (cDC) suppression in PDAC may further impair tumor immunity. We found that low tissue expression of Fms-like tyrosine kinase 3 ligand (Flt3L) partially underlies cDC deficits.

Effect of Caffeinated Chewing Gum on Maximal Strength, Muscular Power, and Muscle Recruitment During Bench Press and Back Squat Exercises.

: This study aims to investigate the effects of caffeinated chewing gum on maximal strength, muscular power, and neural drive to the prime movers during bench press and back squat in resistance-trained men. : Sixteen resistance-trained males participated in a double-blind, randomized trial, chewing either caffeinated gum (4 mg/kg) or placebo gum on two separate occasions, seven days apart. After chewing for 5 min, participants performed a maximal strength test followed by muscular power assessments at 25%, 50%, 75%, and 90% of their one-repetition maximum (1RM), completing with 3, 2, 1, and 1 repetition (s), respectively, for bench press and back squat.

Combined repair technique for patellar tendon rupture using two suture anchors and three transosseous sutures: a retrospective cohort study.

Background: Patellar tendon rupture is an uncommon injury that usually requires surgical repair, but no consensus exists regarding the ideal repair technique. Suture anchor repair and the transosseous suture method have be widely used, but the suture anchor technique may cause anchor pull-out, while the transosseous suture method may result in insufficient suture strength. Therefore, we propose and evaluate the efficacy of combined repair technique with two suture anchors and three transosseous sutures for patellar tendon repairs.

Advantages and Limitations of Zero-Profile Spacers in the Treatment of Cervical Spinal Cord Injury Without Fracture or Dislocation: A 5-Year Retrospective Analysis.

Aim: Treatment options for cervical spinal cord injury without fracture or dislocation (CSCIWFD) remain varied. Although zero-profile spacers are widely used in degenerative cervical conditions, their long-term efficacy in CSCIWFD is underexplored. This study compared the five-year outcomes of zero-profile spacers and plate-cage constructs in CSCIWFD.

Robust prediction of tool-tissue interaction force using ISSA-optimized BP neural networks in robotic surgery.

Background: A persistent problem with robot-assisted minimally invasive surgery is soft tissue damage caused by the exertion of excessive force due to the surgeon’s lack of direct access to the surgical site. A solution to predict clamp force accurately is needed to enhance surgical safety and efficiency.

Methods: The current proposal concerns a deep learning-based solution utilizing a backpropagation neural network (BPNN) optimized by improved sparrow search algorithm (ISSA) to predict clamp force on soft tissue.

Nanocatalytic Neuroprotection and Neurological Recovery Post-Traumatic Brain Injury.

Traumatic brain injury (TBI) is a severe neurological disorder requiring novel therapeutic strategies. We developed ultrasmall catalytic CeZrO nanozymes (CZs) and investigated their neuroprotective potential in combination with nimodipine, a calcium channel blocker. CZs effectively alleviated oxidative stress but were insufficient against calcium-mediated neuronal injury, while nimodipine alone inadequately mitigated oxidative damage.

Integrative analysis of lung adenocarcinoma across diverse ethnicities and exposures.

Lung adenocarcinomas (LUAD) are a pressing global health problem with enduring lethality and rapidly shifting epidemiology. Proteogenomic studies integrating proteomics and post-translational modifications with genomics can identify clinical strata and oncogenic mechanisms, but have been underpowered to examine effects of ethnicity, smoking and environmental exposures, or sex on this heterogeneous disease. This comprehensive proteogenomic analysis of LUAD tumors and matched normal adjacent tissues from 406 patients across diverse geographic and demographic backgrounds explores the impact of understudied driver mutations, prognostic role of chromosomal instability, patterns of immune signaling, differential and sex-specific effects of endogenous mutagens and environmental carcinogens, and pathobiology of early-stage tumors with "late-like" characteristics.

Pharmacokinetics, Safety, and Tolerability of (R)-Ketamine Hydrochloride Injection, a Novel Rapid-Acting Antidepressant, in Healthy Chinese Subjects.

: (R)-ketamine hydrochloride injection is a novel, rapid-acting antidepressant for the treatment of treatment-resistant depression. The aim of this study was to assess the pharmacokinetics, safety, and tolerability of (R)-ketamine hydrochloride injection in healthy Chinese subjects following ascending single intravenous doses ranging from 10.0 mg to 180 mg.

Brain-Wide Spatiotemporally Distinct Traveling Waves Drive Anxiety-Like Behaviors in Mice.

Cortical traveling waves coordinate communication among distributed neural ensembles to modulate brain function and dysfunction through distinct spatiotemporal propagation patterns. However, the brain-wide propagation dynamics of traveling waves from different origins and their roles in regulating behavior remain unclear. Using optogenetics alongside whole-brain fMRI in mice, it is demonstrated that optogenetic activation of the medial prefrontal cortex and primary somatosensory area induces cortical spreading depression (CSD)-like traveling waves.

Microbial diversity characteristics and differential analysis in green turtle nests in the Xisha Islands, South China Sea.

Sea turtles are renowned "living fossils" and ideal flagship marine conservation species. Sea turtles display high fidelity to their nesting grounds, and the quality of nesting grounds is related to the survival of sea turtles, in which microbial diversity, abundance, and potential pathogens are important indicators for assessing the quality of nesting grounds. The Xisha Islands are the largest nesting grounds for green turtles () in China.

The efficacy and mechanisms of low-intensity transcranial ultrasound stimulation on pain: a systematic review of human and animal studies.

Background: Low-intensity transcranial ultrasound stimulation (LITUS) is an emerging non-invasive neuromodulation technique for pain treatment, with the unique ability to modulate deep brain nuclei associated with pain. The aim of this study is to systematically review and summarize the evidence for the efficacy of LITUS in pain management and to elucidate the potential mechanisms underlying its analgesic effects.

Methods: A systematic search was conducted across five databases up to Mar 31st, 2025.

Theoretical framework for confined ion transport in two-dimensional nanochannels.

Quantitative understanding of ion transport mechanism is crucial for numerous applications of two-dimensional (2D) nanochannels, but is far from being resolved. Here, we formulated a theoretical framework for both self-diffusion and electromigration of hydrated monatomic ions in various 2D nanochannels (e.g.