Dual molecularly imprinted nanocomposite with transferrin mediated glioma targeting and cholesterol exhaustion for synergistic cuproptosis/immune checkpoint blockade/immunogenic cell death.

Effective therapies for Glioblastoma (GBM) are often challenging by virtue of the intracranial location of GBM tumors, molecular heterogeneity, high recurrence rate, and overall resistance to treatment. Therefore, we proposed the development of doxorubicin (DOX) loaded molecularly imprinted nanocomposites (DOX@MINPs-TRF/ChO) using transferrin (TRF) and cholesterol (ChO) as dual-template and Cu nanoparticles (Cu@BSNs) as a functional monomer for enhancing the treatment of GBM. The results showed that DOX@MINPs-TRF/ChO specifically and effectively adsorbed TRF in blood circulation and subsequently enhanced the brain tumor targeting capability specific binding with transferrin receptors (TfR) highly expressed on the surface of GL261 cells.

Application of Predilution Plasma Exchange, a new plasmapheresis method for self-anticoagulation, in liver failure.

Introduction: For patients with severe liver failure, citrate accumulation is prone to occur, which increases the risk of death in critically ill patients. To reduce this risk, we created a self-anticoagulation protocol, predilution plasma exchange ( pre-PE ), in which exogenous plasma is replenished into the circuit before the plasma separator. This new method uses citrate in exogenous plasma as an anticoagulant to avoid the use of additional citrate, which would reduce the occurrence of citrate accumulation during PE.

Determination of the standardized ileal amino acid digestibility of corn in 13-day-old broilers and establishment of the prediction equations.

This study was conducted to evaluate apparent ileal amino acid digestibility (AID AA) and standardized ileal amino acid digestibility (SID AA) in corn with different sources fed to broilers and to establish prediction equations of SID AA based on the chemical composition of corn. Ten different sources of corn were evaluated. A total of 660 1-day-old male broilers (Arbor Acres) were randomly divided into 11 treatments (6 replicates, 10 birds per replicate).

Supplement C18:1 in Culture Medium to Improve Survival Rate and Fermentation Activity of Lyophilization of L9.

This study explored the impact of C18:1 fatty acid supplementation in culture medium on the survival rate, fermentation activity, and membrane stability of L9 during freeze-drying. Cells cultured in medium supplemented with C18:1 (0.2 mg/ml) exhibited a higher survival rate (95.

Suppresses CD8 T Cell Effector Function and Intestinal Inflammation.

The role of CD8 T cells in the pathogenesis of ulcerative colitis (UC) remains unclear. Similarly, the posttranscriptional regulation of the highly heterogenic CD8 T cell populations and their effector function in IBD also remains poorly understood. Here, we find that and ) regulate T cell fate, and their expression is higher near damaged colon tissue in patients with IBD compared to controls.

Probiotics restore enteric HDL3 secretion and improve prognosis in patients with end-stage renal disease.

Randomized double-blind trials have shown that probiotic mixtures significantly increase high-density lipoprotein (HDL) levels and reduce the risk of cardiovascular disease mortality in end-stage renal disease (ESRD) patients. Meta-analysis with prospective cohort studies further confirms that elevated HDL is a protective factor for ESRD outcomes. In severe renal injury models, including 5/6 nephrectomy and apolipoprotein E-deficient ( ) mice, probiotics restored cardiac function, mirroring the cardioprotective effects seen in humans.

Sodium-glucose cotransporter 2 inhibitors alleviate renal fibrosis in diabetic kidney disease by inhibiting Hmgcs2 and Btg2 in proximal tubular cells.

Context: Sodium-glucose cotransporter 2 inhibitors (SGLT2i) have been shown to ameliorate renal fibrosis in diabetic kidney disease (DKD), but the mechanism has not been fully explored.

Methods: The single-cell sequencing (scRNA-seq) data were downloaded from the Gene Expression Omnibus (GEO) database, and we selected the tissue data from mice, mice and mice with SGLT2i treatment. The results were also validated by immunofluorescent staining and western blot in vivo and in vitro, respectively.

Preclinical and pilot clinical evaluation of novel dual-modality pet/fluorescence probes targeting FAP for accurate tumor margin delineation.

Purpose: Accurate delineation of tumor margins and maximal safe resection are critical for successful curative oncologic surgery. However, fibroblast activation protein (FAP)-targeted probes suitable for fluorescence imaging remain limited. Here, we developed novel FAP-targeted fluorescent probes to accurately delineate tumor margins and enable rapid intraoperative identification of tumor boundaries in resected specimens.

SGLT2 Inhibitors and Improved Survival in Patients with Diabetes and Acute Myocardial Infarction: Evidence from an Electronic Health Record-Based Cohort Study.

Background: Emerging evidence suggests that sodium-glucose co-transporter-2 (SGLT2) inhibitors reduce cardiovascular events in patients with diabetes mellitus (DM) after acute myocardial infarction (AMI), but evidence in Asian populations remains limited.

Objective: We assessed the impact of SGLT2 inhibitors on in-hospital, 30-day, and 30-day to 1-year mortality in a Northern Chinese real-world cohort.

Methods: An electronic health record-based cohort was constructed from the Tianjin Health and Medical Data Platform from January 2013 to December 2022.

A CD147-targeted small-molecule inhibitor potentiates gemcitabine efficacy by triggering ferroptosis in pancreatic ductal adenocarcinoma.

CD147 has emerged as a promising tumor-specific therapeutic target. Identifying small-molecule inhibitors that promote its proteolysis represents a critical step toward advancing clinical translation, while elucidating its mechanisms of action could further accelerate this process. In this study, we identify dracorhodin perchlorate (DP) as a potent CD147 inhibitor that induces autophagy-dependent degradation.

Unraveling the molecular mechanisms of high hydrostatic pressure in enhancing emulsifying properties: insights from main fractionations in pepper seed protein.

High hydrostatic pressure (HHP) effectively enhances the emulsifying properties of pepper by-product - pepper seed protein (PSP), but the underlying mechanisms require further elucidation. This study investigated the molecular mechanisms of HHP-induced changes in protein emulsification by comparing predominant fractions (pepper seed albumin (PSA) and glutelin (PSG)) obtained by two extraction methods. Results showed that with higher emulsifying activity of PSA increased by 23.

Cycle-configuration descriptors: a novel graph-theoretic approach to enhancing molecular inference.

Inference of molecules with desired activities/properties is one of the key and challenging issues in cheminformatics and bioinformatics. For that purpose, our research group has recently developed a state-of-the-art framework mol-infer for molecular inference. This framework first constructs a prediction function for a fixed property using machine learning models, which is then simulated by mixed-integer linear programming to infer desired molecules.

Elucidating the metabolic pathway and initial degradation gene for -chloro--xylenol biodegradation in DMU114.

Unlabelled: The antimicrobial agent -chloro--xylenol (PCMX), an emerging environmental pollutant, poses ecological risks; however, its biodegradation mechanisms remain unresolved. Here, we elucidate the metabolic pathway and functional genes involved in the initial catabolic step of PCMX in a newly isolated bacterium, DMU114. Pure-culture and synthetic consortium assays confirmed the pivotal role of in PCMX degradation, despite its relatively low abundance in the PCMX-enriched consortium.

Plasma proteomic signature for preoperative prediction of microvascular invasion in HCC.

Background & Aims: Microvascular invasion (MVI) is a major determinant of poor prognosis in hepatocellular carcinoma (HCC). However, reliable non-invasive biomarkers for the preoperative evaluation and diagnosis of MVI are urgently needed in clinical practice.

Methods: Plasma samples were collected from 160 patients with HCC (80 MVI-positive and 80 MVI-negative) from four medical centers.

Engineered secreting a glucagon-like peptide-1-inducing protein P9 for amelioration of obesity in mice.

Unlabelled: This study investigated the anti-obesity potential of engineered to constitutively secrete P9 (Amuc_1631), a protein from . Our findings show that the engineered strain P11P9 effectively secretes P9 and significantly increases GLP-1 production in NCI-H716 enteroendocrine cells, with a 2.65-fold rise compared to controls.

Preliminary analysis of the diagnostic value and mechanism of action of TGFbI and S100A4 in hepatocellular carcinoma.

Background: The aim was to analyse the diagnostic value of transforming growth factor-beta-induced protein (TGF I) and S100 calcium-binding protein A4 (S100A4) on hepatocellular carcinoma (HCC) and to explore further the effects of TGF I and S100A4 on ferroptosis in HCC cells.

Methods: We retrospectively analysed 76 patients with HCC admitted to our hospital from October 2022 to June 2023 and detected the differences in the expression of TGF I and S100A4 in cancerous tissues and paracancerous tissues to analyse their diagnostic and prognostic assessment value for HCC. Additionally, the HCC cell line HepG2 was purchased and transfected with TGF I and S100A4 abnormal expression plasmids to check changes in cell viability, oxidative stress damage, mitochondrial damage, and ferroptosis.

Unidirectional electric field enables reversible ferroelectric domain engineering.

The deterministic control of ferroelectric polarization via an external field is critical for advancing the technologies of modern information storage. Conventionally, reversible and cyclic polarization switching in ferroelectric materials requires bipolar electric fields. The present work demonstrates the efficient reversible and cyclic ferroelectric domain switching under a unipolar electric field in van der Waals ferroelectric CuInPS, enabled by Cu-ion migration across van der Waals gaps.

Evolutionary Conservation and Immunoregulatory Function of LOXL3 Gene in Lamprey.

Lysyl oxidase (LOX) family enzymes play a pivotal role in extracellular matrix (ECM) remodeling and tissue homeostasis, and have evolved diverse functions in vertebrate immunity and metabolism. However, the evolutionary trajectory of these multifunctional roles-particularly in jawless vertebrates-remains obscure. Here, we investigate LOXL3 in the lamprey (Lethenteron reissneri), a jawless vertebrate that retains ancestral features of early vertebrate evolution.

Association between e-cigarette use and COVID-19 diagnosis: a systematic review and meta-analysis.

Background: The impact of e-cigarettes on COVID-19 remains unclear. This study aims to assess the relationship between e-cigarette use and COVID-19 diagnosis and other related outcomes.

Methods: This systematic review and meta-analysis searched studies from 2019 to April 2nd, 2024, in Medline (via OVID), EMBASE, Scopus and the Cochrane Central Register of Controlled Trials for eligible observational studies.

Unveiling the relationship between counterion size and spin crossover dynamics in hexadentate Schiff-base manganese(III) complexes.

Understanding the relationship between molecular packing/interaction and the spin crossover property is essential for advancing solid-state molecular memory devices. In this work, a series of hexadentate Schiff-base manganese(III) complexes ([Mn(4F-sal323)]X, X = ClO (1); X = AsF (2); X = PF (3); X = ReO (4) and X = NO (5)) were synthesized and characterized. Magnetic studies showed that complex 1 exhibited an abrupt spin transition at 90 K with a 10 K wide thermal hysteresis, while complexes 2-5 exhibited gradual and incomplete spin transition with no hysteresis.

Recent preparation and applications of chiral pillar[5]arene-based functional materials.

Pillar[5]arenes were first synthesized and reported in 2008 by Ogoshi Due to their rigid cavity structures, rich host-guest interactions and ease of functionalization, pillar[5]arenes have garnered considerable attention in the fields of supramolecular chemistry, material science and stereochemistry. However, they face significant challenges in obtaining stable enantiomers due to the presence of rotational single bonds within the cavity structure, which leads to a high predisposition for racemization. This review provides a comprehensive analysis of the synthesis of pillar[5]arene-based macrocyclic chiral materials and their applications over the past decade, including circularly polarized luminescent materials, chirality memory materials, chiral organic nanotube materials, and chiral molecular recognition and separation.

Modulation of the Surface Catalytic Activity of Boron-Doped Cobalt Oxide with Crystalline/Amorphous Interfaces for High-Stability Acetone Detection.

Enhancing the gas-solid interface interaction between sensing materials and O is promising for the development of high-performance metal oxide-based chemiresistive gas sensors. Nevertheless, high-performance gas sensors have not been developed owing to the lack of a deep understanding of the sensing mechanism with regards to gas-solid interface interactions. In this study, boron-doped cobalt oxide (B-CoO) with crystalline/amorphous interfaces was synthesized for acetone detection.

Unravelling the Web of Life: Incomplete Lineage Sorting and Hybridisation as Primary Mechanisms Over Polyploidisation in the Evolutionary Dynamics of Pear Species.

The traditional Tree of Life (ToL) model is increasingly challenged by the Web of Life (WoL) paradigm, which offers a more accurate depiction of organismal phylogeny, particularly in light of the incongruences often observed between gene and species trees. However, the absence of a standardised method for resolving evolutionary mechanisms - such as Incomplete Lineage Sorting (ILS), hybridisation, introgression, polyploidisation, and whole-genome duplication - remains a significant obstacle in defining the WoL. Characterised by extensive hybridisation events, the pear genus Pyrus provides an ideal model for exploring these complexities.

Tumor-derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma.

Hepatocellular carcinoma (HCC) is characterized by aberrant tumor vasculature and an immunosuppressive tumor microenvironment (TME), both of which compromise immunotherapy efficacy while promoting circulating tumor cell (CTC) dissemination and immune escape. Here, we aimed to identify potential therapeutic targets for remodeling aberrant tumor vasculature by analyzing CTCs from early-stage HCC patients. HCC tissue samples derived from patients with elevated CTC counts demonstrated significant CCL16 downregulation accompanied by vascular structural abnormalities and an immunosuppressive TME.

METTL3 facilitates colorectal cancer growth through altering the abundance of intestinal Akkermansia muciniphila.

Colorectal cancer (CRC) is a prevalent malignant tumor that poses a significant threat to human health; however, the precise mechanism underlying its onset remains elusive. In this study, we utilized metagenomic sequencing to reveal the dysregulation of intestinal microbiota caused by CRC. Single-cell sequencing data showed elevated mRNA expression of methyltransferase-like protein 3 (METTL3) in CRC, which was correlated with the abundance of intestinal microbiota.