Plasma proteomic signature for preoperative prediction of microvascular invasion in HCC.

Background & Aims: Microvascular invasion (MVI) is a major determinant of poor prognosis in hepatocellular carcinoma (HCC). However, reliable non-invasive biomarkers for the preoperative evaluation and diagnosis of MVI are urgently needed in clinical practice.

Methods: Plasma samples were collected from 160 patients with HCC (80 MVI-positive and 80 MVI-negative) from four medical centers.

Parental Social Support and Preschoolers' Oral Health Behaviours: The Mediating Role of Parental Stress and Self-Efficacy.

Introduction And Aims: Oral health behaviours (OHBs) of preschoolers are crucial for preventing early childhood caries (ECC); however, the relationship between parental/caregivers' factors and the OHBs of Chinese preschoolers has not been clearly defined. This study aimed to explore the relationship between parental/caregivers' social support and the OHBs of Chinese preschoolers, and whether parental stress and self-efficacy mediate this relationship serially.

Methods: This cross-sectional study was conducted in Minhang District, Shanghai, from December 2023 to March 2024, among 2393 participants recruited from parents or caregivers of preschoolers from 46 kindergartens.

A biodegradable magnetic microrobot based on alginate/GelMA microcapsules for delivery of chondroitin sulfate for cartilage tissue repair.

Defects of articular cartilage can lead to osteoarthritis, but the effective repair of damaged cartilage is still a clinical challenge. Microrobots based on smart hydrogel can deliver drugs to cartilage defects in a minimally invasive manner. Herein, biodegradable microrobots were fabricated based on spherical microcapsules composed of sodium alginate (SA), gelatin methacrylate (GelMA) and iron oxide nanoparticles (FeO) using interficial shearing method with high mass production.

Co-elevated CO concentration and temperature enhance the carbon assimilation and lipid metabolism in a high-oil soybean (Glycine max (L.) Merr.) variety.

Global climate changes, such as elevated CO concentrations (eCO) and rising temperatures, trigger complex interactions that collectively affect soybean growth and seed quality. To investigate the effects of eCO and elevated temperature on carbon and lipid metabolism in soybeans, the high-oil Zhonghuang35 (ZH35) and the low-oil soybean Jindazaohuang2 (JZ2) were used in this study. Four treatments were set up in the controlled air chambers.

Strong plasmon coupled gold nanoantennas with closed hot-spots enabled ultrasensitive lateral flow immunoassay of porcine epidemic diarrhea virus.

Porcine epidemic diarrhea virus (PEDV) is the etiological agent of porcine epidemic diarrhea (PED), which is an acute and devastating enteric disease in suckling piglets and shows cross-species transmission ability between pigs and humans as well. The accurate and timely detection of PEDV infection is essential for the control and prevention of viral transmission and outbreaks. Here, a strong plasmon coupled gold nanoantennas have been developed by Ag induced gold nanoparticles (AuNPs) assembly for surface-enhanced Raman scattering (SERS) integrated lateral flow immunoassay (LFIA) toward rapid and ultrasensitive determination of PEDV.

Enhanced Therapeutic Effect of IL-10-ADSCs on Rabbit Autoimmune Dacryoadenitis By Suppressing T Follicular Helper Cell Responses Via miR-142-5p/RC3H1 Axis.

Purpose: Mesenchymal stem cells (MSCs) represent a promising therapeutic strategy in clinical research for dry eye, and their immunomodulatory effects can be enhanced through genetic modification. In this study, we constructed interleukin-10 (IL-10) gene-modified adipose-derived MSCs (IL-10-ADSCs) and investigated their protective effects and underlying mechanisms on rabbit autoimmune dacryoadenitis, an animal model of autoimmune dry eye.

Methods: ADSCs were isolated from rabbit adipose tissue and transduced with IL-10 overexpressing lentivirus.

Conducting polymer functionalized Cu-metal organic framework-based electrochemical immunosensor for rapid and sensitive quantitation of Escherichia coli O157:H7.

Escherichia coli (E. coli) O157:H7 is an important food-borne pathogen that can cause hemorrhagic diarrhea and enteritis in humans and animals. Realizing the rapid quantitation of E.

The transcriptional landscape and clinico-biological characterization of human endogenous retroviruses in esophageal squamous cell carcinoma.

Human endogenous retroviruses (HERVs) are emerging as critical elements in host genomic regulation. Aberrant HERV transcription has been implicated in developmental and tissue-specific aging and pathological processes. In this study, we presented a comprehensive locus-specific characterization of the HERV expression landscape in esophageal squamous cell carcinoma (ESCC).

Association of maternal mineral status with the risk of preterm birth: a retrospective cohort study.

Background: There has been a gradual increase in the proportion of preterm birth in China during the past several decades. Maternal malnutrition is a significant determinant for preterm birth. Nevertheless, comprehensive studies investigating serum mineral levels during pregnancy associated with preterm birth remain scarce.

RTCpredictor: identification of read-through chimeric RNAs from RNA sequencing data.

Read-through chimeric RNAs are being recognized as a means to expand the functional transcriptome and contribute to cancer tumorigenesis when mis-regulated. However, current software tools often fail to predict them. We have developed RTCpredictor, utilizing a fast ripgrep tool to search for all possible exon-exon combinations of parental gene pairs.

Effect of Glycolipids Application Combined with Nitrogen Fertilizer Reduction on Maize Nitrogen Use Efficiency and Yield.

Microbial-driven N turnover is important in regulating N fertilizer use efficiency through the secretion of metabolites like glycolipids. Currently, our understanding of the potential of glycolipids to partially reduce N fertilizer use and the effects of glycolipids on crop yield and N use efficiency is still limited. Here, a three-year in situ field experiment was conducted with seven treatments: no fertilization (CK); chemical N, phosphorus and potassium (NPK); NPK plus glycolipids (N+PKT); and PK plus glycolipids with 10% (0.

: A Sex-Specific Chimeric RNA and Its Role in Immune Sexual Dimorphism.

RNA processing mechanisms, such as alternative splicing and RNA editing, have been recognized as critical means to expand the transcriptome. Chimeric RNAs formed by intergenic splicing provide another potential layer of RNA diversification. By analyzing a large set of RNA-Seq data and validating results in over 1,200 blood samples, we identified , a female-specific chimeric transcript.

Exosomes from Intrahepatic Cholestasis of Pregnancy Induce Cell Apoptosis Through the miRNA-6891-5p/YWHAE Pathway.

Background: Intrahepatic cholestasis of pregnancy (ICP) is associated with adverse pregnancy outcomes; however, the underlying mechanisms are not fully understood.

Aims: This study aimed to determine the role of exosomal miR-6891-5p in placental trophoblast dysfunction in ICP and identify new biomarkers for ICP diagnosis.

Methods: Serum samples were collected from ICP patients and healthy pregnant women, and serum exosomes were extracted and identified.

The role of ERp29/FOS/EMT pathway in excessive apoptosis of placental trophoblast cells in intrahepatic cholestasis of pregnancy.

Background: Abnormal bile acid metabolism leading to changes in placental function during pregnancy. To determine whether endoplasmic reticulum protein 29 (ERp29) can mediate the pregnancy effects of cholestasis by altering the level of trophoblast cell apoptosis.

Methods: ERp29 in serum of 66 intrahepatic cholestasis of pregnancy (ICP) pregnant women and 74 healthy were detected by ELISA.

A protein-encoding CCDC7 circular RNA inhibits the progression of prostate cancer by up-regulating FLRT3.

Circular RNAs (circRNAs) are a family of endogenous RNAs that have become a focus of biological research in recent years. Emerging evidence has revealed that circRNAs exert biological functions by acting as transcriptional regulators, microRNA sponges, and binding partners with RNA-binding proteins. However, few studies have identified coding circRNAs, which may lead to a hidden repertoire of proteins.

Elevated CO concentration enhances drought resistance of soybean by regulating cell structure, cuticular wax synthesis, photosynthesis, and oxidative stress response.

The atmospheric [CO] and the frequency and intensity of extreme weather events such as drought are increased, leading to uncertainty to soybean production. Elevated [CO] (eCO) partially mitigates the adverse effects of drought stress on crop growth and photosynthetic performance, but the mitigative mechanism is not well understood. In this study, soybean seedlings under drought stress simulated by PEG-6000 were grown in climate chambers with different [CO] (400 μmol mol and 700 μmol mol).

The effect of anxiety, depression, and structural social capital on life satisfaction among people with hearing disabilities: a cross-sectional study in Shanghai, China.

Background: Life satisfaction (LS) serves as a crucial indicator of social wellbeing and plays a significant role in formulating strategies aimed at enhancing health outcomes among the hearing-disabled population. This study aimed to examine the effect of anxiety, depression, and structural social capital on life satisfaction among people with hearing disabilities in Shanghai, China.

Methods: A cross-sectional study was conducted in Shanghai, China.

Gold nanoparticle dimer-based immunochromatography for in situ ultrasensitive detection of porcine epidemic diarrhea virus.

The low detection sensitivity of lateral-flow immunochromatography assay (LFIA) based on spherical gold nanoparticle (AuNP) limits its wide applications. In the present study, AuNP dimers with strong plasma scattering and robust signal output were synthesized via the Ag ion soldering (AIS) strategy and used as labeled probes in LFIA to boost the sensitivity without any extra operation process and equipment. The established LFIA exhibited high sensitivity with a limit of detection (LOD) of 2.

Identification and validation of potential hypoxia-related genes associated with coronary artery disease.

Coronary artery disease (CAD) is one of the most life-threatening cardiovascular emergencies with high mortality and morbidity. Increasing evidence has demonstrated that the degree of hypoxia is closely associated with the development and survival outcomes of CAD patients. However, the role of hypoxia in CAD has not been elucidated.

Identification of INHBA as a potential biomarker for gastric cancer through a comprehensive analysis.

Inhibin subunit beta A (INHBA) is a member of the transforming growth factor-beta (TGF-β) superfamily that plays a fundamental role in various cancers. However, a systematic analysis of the exact role of INHBA in patients with gastric cancer (GC) has not yet been conducted. We evaluated the expression levels of INHBA and the correlation between INHBA and GC prognosis in GC.

Construction of the panoptosis-related gene model and characterization of tumor microenvironment infiltration in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is the most common fatal cancer worldwide, patients with HCC have a high mortality rate and poor prognosis. PANoptosis is a novel discovery of programmed cell death associated with cancer development. However, the role of PANoptosis in HCC remains obscure.