Polyphenol Oxidase-Like Nanozymes.

Nanozymes are nanomaterials with enzyme-like activities. Over the past decade, a steadily growing body of research on polyphenol oxidase-like (PPO-like) nanozymes, a subgroup of oxidase-like nanozymes, has suggested their increasing prominence in nanozymology. Most importantly, PPO-like nanozymes have developed a unique, well-defined, and systematic design philosophy along with a corresponding catalytic mechanism.

Incorporating Stem Cell-Derived Small Extracellular Vesicles into a Multifunctional OHAMA-EPL Hydrogel Spray for Rapid Healing of Infected Skin Wounds.

Wound healing is a significant clinical concern that can be hindered by excessive inflammation, infection, and insufficient regeneration. Small extracellular vesicles (sEVs) derived from stem cells have been extensively studied for wound healing, but their efficient delivery and controllable release in the targeted tissues remain a technical challenge. A stem cell-derived sEVs incorporated dual-network hydrogel spray system is prepared for the rapid healing of infected full-thickness skin wounds for the first time.

Diagnosis of deep vein thrombosis from γ-butylbetaine and L-carnitine in plasma and urine based on untargeted and targeted metabolomics.

Deep vein thrombosis (DVT) is a significant contributor to the global disease burden. This study aimed to identify metabolic biomarkers to compensate for the limitation in specificity and singular nature of D-dimer test. UHPLC-MS was used to examine γ-butylbetaine and L-carnitine levels in clinical and rat blood and urine at different stages of DVT along with analysis of their diagnostic titer and correlation analyses with DVT.

The effect of chloroquine on cervical cancer via the PI3K/AKT/MDM2 pathway.

Background: Cervical cancer (CC), the most prevalent malignant tumor in the female reproductive system, is characterized by its high incidence and mortality rates. Chloroquine(CQ), a 4-aminoquinoline drug originally used to prevent and treat malaria, has gained recognition as an adjuvant anti-cancer agent. However, whether chloroquine exhibits anti-CC activity and its underlying mechanisms remain unclear.

Strong plasmon coupled gold nanoantennas with closed hot-spots enabled ultrasensitive lateral flow immunoassay of porcine epidemic diarrhea virus.

Porcine epidemic diarrhea virus (PEDV) is the etiological agent of porcine epidemic diarrhea (PED), which is an acute and devastating enteric disease in suckling piglets and shows cross-species transmission ability between pigs and humans as well. The accurate and timely detection of PEDV infection is essential for the control and prevention of viral transmission and outbreaks. Here, a strong plasmon coupled gold nanoantennas have been developed by Ag induced gold nanoparticles (AuNPs) assembly for surface-enhanced Raman scattering (SERS) integrated lateral flow immunoassay (LFIA) toward rapid and ultrasensitive determination of PEDV.

Mesenchymal Stem Cell-Derived Extracellular Vesicles Embedded in a Self-Adaptive Multifunctional Hydrogel for Rapid Healing of Infected Wounds.

Wound healing represents a major clinical concern, disrupted by cellular dysfunction due to infection, metabolic imbalance, and immune response, resulting in physical and economic burden to the patients. Despite the widespread recognition of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in wound healing research, ensuring their release and retention at the wound site and maximizing their therapeutic efficacy remain significant challenges. To address these issues, a self-adaptive multifunctional hydrogel is introduced that allows the self-adaptive release of MSC-EVs at the wound sites.

Purification, immobilization, evolution, and characterization of D-allulose 3-epimerase from Antarctic Microbacterium.

D-allulose is a rare, low-calorie sugar substitute with multiple physiological functions and is widely used in food and pharmaceutical industries. D-allulose is primarily produced by the catalytic conversion of d-fructose via D-allulose 3-epimerase (DAEase). In this study, a DAEase gene, dpema4, was isolated from an Antarctic bacterium and expressed in Escherichia coli.

Advances in Tracing Techniques: Mapping the Trajectory of Mesenchymal Stem-Cell-Derived Extracellular Vesicles.

Mesenchymal stem-cell-derived extracellular vesicles (MSC-EVs) are nanoscale lipid bilayer vesicles secreted by mesenchymal stem cells. They inherit the parent cell's attributes, facilitating tissue repair and regeneration, promoting angiogenesis, and modulating the immune response, while offering advantages like reduced immunogenicity, straightforward administration, and enhanced stability for long-term storage. These characteristics elevate MSC-EVs as highly promising in cell-free therapy with notable clinical potential.

Identification of PTPRC as a potential serum biomarker in rheumatoid arthritis using bioinformatics analysis and molecular docking.

Background: Rheumatoid arthritis (RA) is a multifactorial autoimmune disease affecting joints. Early and accurate diagnosis is important for initiating the correct treatment and preventing long-term complications.

Methods: Microarray expression data of synovial tissues from people with osteoarthritis and healthy populations were downloaded from the Gene Expression Omnibus (GEO) database.

Rethinking Appearance-Based Deep Gait Recognition: Reviews, Analysis, and Insights From Gait Recognition Evolution.

Gait recognition is a prominent biometric recognition technique extensively employed in public security. Appearance-based and model-based gait recognition are two categories of methods commonly used. Specifically, appearance-based methods, which use silhouettes to represent body information, typically outperform model-based methods that rely on skeleton data, making them more popular.

Functional near-infrared spectroscopy for the assessment and treatment of patients with disorders of consciousness.

Background: Advances in neuroimaging have significantly enhanced our understanding of brain function, providing critical insights into the diagnosis and management of disorders of consciousness (DoC). Functional near-infrared spectroscopy (fNIRS), with its real-time, portable, and noninvasive imaging capabilities, has emerged as a promising tool for evaluating functional brain activity and nonrecovery potential in DoC patients. This review explores the current applications of fNIRS in DoC research, identifies its limitations, and proposes future directions to optimize its clinical utility.

Unveiling urinary extracellular vesicle mRNA signature for early diagnosis and prognosis of bladder cancer.

Bladder cancer (BC) ranks as one of the most prevalent cancers. Its early diagnosis is clinically essential but remains challenging due to the lack of reliable biomarkers. Extracellular vesicles (EVs) carry abundant biological cargoes from parental cells, rendering them as promising cancer biomarkers.

Systematic evaluation of methylation-based cell type deconvolution methods for plasma cell-free DNA.

Background: Plasma cell-free DNA (cfDNA) is derived from cellular death in various tissues. Investigating the tissue origin of cfDNA through cell type deconvolution, we can detect changes in tissue homeostasis that occur during disease progression or in response to treatment. Consequently, cfDNA has emerged as a valuable noninvasive biomarker for disease detection and treatment monitoring.

Efficacy of melatonin for prolonged disorders of consciousness: a double-blind, randomized clinical trial.

Background: Sleep is essential for the recovery of patients with disorders of consciousness (DoC). However, few approaches targeting sleep were applied. Melatonin has been shown to enhance sleep efficiency with virtually no side effects.

Screening of potential biomarkers of osteoarthritis: a bioinformatics analysis.

Background: Osteoarthritis (OA) is the most common joint disease worldwide, with an age-associated increasing in both incidence and prevalence. However, early diagnosis of OA is a challenge due to the lack of effective biomarkers. This study aimed to identify new biomarkers and mechanisms of OA.

Gypenosides alleviate oxidative stress in the hippocampus, promote mitophagy, and mitigate depressive-like behaviors induced by CUMS via SIRT1.

Ethnopharmacological Relevance: The use and efficacy of Gynostemma [Gynostemma pentaphyllum (Thunb.) Makino], a versatile traditional Chinese herb, was first documented in the renowned pharmacopoeia, "Compendium of Materia Medica". Gypenosides (Gps), saponin components are the primary constituents responsible for its biological activities and clinical effects, which include antioxidant, immunoregulatory, antitumor, and neuroprotective properties.

Investigations on the shock wave induced by collapse of a toroidal bubble.

When bubbles collapse near a wall, they typically experience an asymmetric deformation. This collapse leads to the creation of a jet that strikes the bubble interface, causing the formation of a toroidal bubble and the subsequent release of a water-hammer shock. In this study, we present a systematic analysis of the collapse of a toroidal bubble in an open field or adjacent to a flat wall using high-fidelity numerical simulation.

Identification of mitochondria-related gene biomarkers associated with immune infiltration in acute myocardial infarction.

Mitochondrial dysfunction has been known to contribute to the worsening of acute myocardial infarction (AMI). We screened differentially expressed genes (DEGs) between AMI and healthy individuals based on the GSE66360 dataset. We took the intersection of the obtained DEGs with 1,136 mitochondria-related genes.

A Single-Step Method for Harvesting Influenza Viral Particles from MDCK Cell Culture Supernatant with High Yield and Effective Impurity Removal.

Influenza vaccines, which are recommended by the World Health Organization (WHO), are the most effective preventive measure against influenza virus infection. Madin-Darby canine kidney (MDCK) cell culture is an emerging technology used to produce influenza vaccines. One challenge when purifying influenza vaccines using this cell culture system is to efficiently remove impurities, especially host cell double-stranded DNA (dsDNA) and host cell proteins (HCPs), for safety assurance.

The Diagnostic Accuracy Between Radiomics Model and Non-radiomics Model for Preoperative of Microvascular Invasion of Solitary Hepatocellular Carcinoma: A Systematic Review and Meta-analysis.

Rationale And Objectives: Microvascular invasion (MVI) is a key prognostic factor for hepatocellular carcinoma (HCC). The predictive models for solitary HCC could potentially integrate more comprehensive tumor information. Owing to the diverse findings across studies, we aimed to compare radiomic and non-radiomic methods for preoperative MVI detection in solitary HCC.

Prediction of pathological activity in Crohn's disease based on dual-energy CT enterography.

Purpose: To explore the feasibility of predicting the pathological activity of Crohn's disease (CD) based on dual-energy CT enterography (DECTE).

Methods: The clinical, endoscopic, imaging and pathological data of 55 patients with CD scanned by DECTE were retrospectively analyzed; the pathological results were used as a reference standard to classify the diseased bowel segments into active and inactive phases. The normalized iodine concentration (NIC), energy-spectrum curve slope K, dual energy index (DEI), fat fraction (FF) of the arterial phases and venous phases were compared.

How well do neural signatures of resting-state EEG detect consciousness? A large-scale clinical study.

The assessment of consciousness states, especially distinguishing minimally conscious states (MCS) from unresponsive wakefulness states (UWS), constitutes a pivotal role in clinical therapies. Despite that numerous neural signatures of consciousness have been proposed, the effectiveness and reliability of such signatures for clinical consciousness assessment still remains an intense debate. Through a comprehensive review of the literature, inconsistent findings are observed about the effectiveness of diverse neural signatures.

Recent Advances in Hepatic Metabolic Regulation by the Nuclear Factor Rev-erbɑ.

Rev-erbɑ (NR1D1) is a nuclear receptor superfamily member that plays a vital role in mammalian molecular clocks and metabolism. Rev-erbɑ can regulate the metabolism of drugs and the body's glucose metabolism, lipid metabolism, and adipogenesis. It is even one of the important regulatory factors regulating the occurrence of metabolic diseases (e.

Rapid and visual identification of β-lactamase subtypes for precision antibiotic therapy.

The abuse of antibiotics urgently requires rapid identification of drug-resistant bacteria at the point of care (POC). Here we report a visual paper sensor that allows rapid (0.25-3 h) discrimination of the subtypes of β-lactamase (the major cause of bacterial resistance) for precision antibiotic therapy.