SPRY1 Interaction with CBL Promotes Vimentin Stabilization to Enhance Malignant Progression and Metastasis in Wilms Tumor.

Background: SPRY1 from the Sprouty family is frequently overexpressed in many tumors and is accompanied by poor prognosis and metastatic progression. However, its role in the tumorigenesis of Wilms tumor (WT) remains unknown. The aim of this study is to elucidate the functional role of SPRY1 in the oncogenesis of Wilms tumor.

Phase Equilibrium Regulation in ZIF-67-Derived Electrocatalysts: Degradation Mechanism and Stability Enhancement for Oxygen Evolution Reaction.

Metal-organic frameworks (MOFs) like ZIF-67 are promising electrocatalysts due to their tunable structures and porosity, but their instability in aqueous electrolytes requires a deeper understanding. This study investigates the structural evolution and degradation mechanism of ZIF-67 during the oxygen evolution reaction (OER) in alkaline media. Using atomic-resolution identical-location transmission electron microscopy, we reveal its transformation pathway: ZIF-67 first converts to Co(OH), then progressively evolves into catalytically active CoOOH and inactive CoO species, ultimately establishing a dynamic three-phase equilibrium under operational conditions.

A comparative pharmacological study of three Chinese traditional medicines found to be the key functional bacterium of Franch. and Cortex against colitis.

Background: Sanhuang refers to the three cold-natured and bitter-flavored traditional Chinese medicines, namely, Georgi (HuangQin, HQ), Franch. (HuangLian, HL), and Cortex (HuangBo, HB). Although similar in drug properties, they are traditionally used to treat different dampness-heat syndromes belonging to the Upper Jiao (lung and heart diseases), the Middle Jiao (stomach and intestine diseases), and the Lower Jiao (intestine, kidney, and bladder diseases).

Multi-enzyme system for efficient bioamination of lignin-derived vanillin into vanillylamine: Enzyme structural insights and pyruvate removal strategy for enhanced biocatalytic performance.

Recently, there has been great interest to synthesize amines from biomass-based molecules through the biological amination with ω-transaminase under the mild conditions. In this research, pyruvate decarboxylase (PDC) was co-expressed with ω-transaminase HNILQE in a recombinant E. coli strain (HNILQE-PDC) to create a multi-enzyme pyruvate removal system.

Prognostic and Therapeutic Value of Metabolism-Related Genes in Nephroblastoma: A Focus on the Key Gene NNMT and Its Regulative Effect on Metabolism.

Metabolic reprogramming plays an essential role in the initiation and aggressiveness of malignant tumors. This study aims to establish a prognostic signature for Wilms tumor (WT) based on metabolism-related genes (MRGs) and to explore potential molecular mechanisms. RNA sequencing data of WT samples and MRGs were sourced from GEO, TCGA and KEGG, respectively.

Expression of the immune gene related to energy metabolism (NDRG1) in Wilms' tumor and its correlation with prognosis.

Background And Objective: This study aimed to investigate the expression of NDRG1 in Wilms' tumor and analyze its correlation with WT prognosis, thereby elucidating its role and potential mechanisms in the onset and progression of WT.

Methods: The expression level of NDRG1 was evaluated in the WT TARGET cohort and validated using the GSE138869 cohort. Univariate and multivariate Cox regression analyses, along with decision curve analysis and nomogram construction, were executed for NDRG1.

ENC1 Promotes the Malignant Progression and Metastasis by Suppressing TRIM21 Mediated Vimentin Degradation in Wilms Tumor.

Ectodermal neural cortex 1 (ENC1) is significantly upregulated in various cancers and shows a positive correlation with poor prognosis and advanced clinical stages, such as colorectal cancer, endometrial cancer and breast cancer. However, the role of ENC1 in Wilms tumor (WT) has not been previously reported. In this study, we conducted several in vitro functional experiments and established xenograft models to confirm the oncogenic potential of ENC1.

The signature based on interleukin family and receptors identified IL19 and IL20RA in promoting nephroblastoma progression through STAT3 pathway.

Wilms tumor (WT) is a common renal malignancy in pediatric patients. Interleukin (receptors) (IL(R)s) play significant roles in tumor biology, however, their specific involvement in WT remains inadequately understood. We employed univariate Cox regression analysis to screen for certain IL(R) genes associated with prognosis and then analyzed their expression patterns.

An exploration into the construction and application of a pediatric surgery digital question bank based on the WeChat official accounts platform.

Backgroud: In the field of medical education, the innovation and optimization of teaching methods have always been the key to improving the quality of education. The popularization of the WeChat platform has provided a broader application space for question banks, especially digital question banks. To improve the teaching quality of pediatric surgery, a digital question bank for pediatric surgery was constructed based on the WeChat platform and applied to the clinical teaching of pediatric surgery.

Nanoscale high-entropy surface engineering promotes selective glycerol electro-oxidation to glycerate at high current density.

Selective production of valuable glycerol chemicals, such as glycerate (which serves as an important chemical intermediate), poses a significant challenge due to the facile cleavage of C-C bonds and the presence of multiple reaction pathways. This challenge is more severe in the electro-oxidation of glycerol, which requires the development of desirable electrocatalysts. To facilitate the glycerol electro-oxidation reaction to glycerate, here we present an approach utilizing a high-entropy PtCuCoNiMn nanosurface.

Two decades of materials research excellence at NIMTE: celebrating the 20th anniversary of Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences.

In celebration of the 20th anniversary of Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences (NIMTE, CAS), has published this collection showcasing some of the recent and impactful research from the institute.

Efficient amine-assisted CO hydrogenation to methanol co-catalyzed by metallic and oxidized sites within ruthenium clusters.

Amine-assisted two-step CO hydrogenation is an efficient route for methanol production. To maximize the overall catalytic performance, both the N-formylation of amine with CO (i.e.

Association between prophylactic aspirin use and hypertension using data from NHANES 2011-2018.

Aspirin may be necessary for some patients with cardiovascular disease, but previous studies on the use and dosage of aspirin and the association with hypertension have been inadequate. The results of existing studies have been somewhat inconsistent. Our study was designed to assess the association between prophylactic aspirin use and hypertension in U.

Design of 3d transition metal-embedded asymmetric HMoCF for electrocatalytic conversion of N to NH.

The electrochemical reduction of N to NH (NRR) is challenging due to the lack of efficient catalysts under mild conditions. We constructed a series of 3d-transition-metal (3d-TM)-embedded asymmetric 2D MXene HMoCF with one H or F vacancy (H or F) based on first-principles calculation. Due to the strong steric effect of the surface-covered H or F terminals, N is favored to be adsorbed on H or F through the end-on mode rather than the side-on mode.

Rational mutagenesis of an epoxide hydrolase and its structural mechanism for the enantioselectivity improvement toward chiral ortho-fluorostyrene oxide.

Chiral (S)-o-fluorostyrene oxide (oFSO) and vicinal diol (R)-o-fluorophenylethane-1,2-diol (oFPED) are important intermediates for synthesizing treatments for neuropathic diseases. This study aimed to engineer Aspergillus usamii epoxide hydrolase (AuEH2) through a rational mutagenesis strategy to customize high enantioselectivity mutant for rac-oFSO. Out of 181 single-site mutants screened, six showed elevated enantiomeric ratio (E value) ranging from 32 to 108 according to E value and activity mutability landscapes.

Self-cleaning electrode for stable synthesis of alkaline-earth metal peroxides.

Alkaline-earth metal peroxides (MO, M = Ca, Sr, Ba) represent a category of versatile and clean solid oxidizers, while the synthesis process usually consumes excessive hydrogen peroxide (HO). Here we discover that HO synthesized via two-electron electrochemical oxygen reduction (2e ORR) on the electrode surface can be efficiently and durably consumed to produce high-purity MO in an alkaline environment. The crucial factor lies in the in-time detachment of in situ-generated MO from the self-cleaning electrode, where the solid products spontaneously detach from the electrode to solve the block issue.

Strontium Doped IrO Triggers Direct O-O Coupling to Boost Acid Water Oxidation Electrocatalysis.

The discovery of efficient and stable electrocatalysts for the oxygen evolution reaction (OER) in acidic conditions is crucial for the commercialization of proton-exchange membrane water electrolyzers. In this work, we propose a Sr(OH)-assisted method to fabricate a (200) facet highly exposed strontium-doped IrO catalyst to provide available adjacent iridium sites with lower Ir-O covalency. This design facilitates direct O-O coupling during the acidic water oxidation process, thereby circumventing the high energy barrier associated with the generation of *OOH intermediates.

Engineering an Epoxide Hydrolase for Chemoenzymatic Asymmetric Synthesis of Chiral Triazole Fungicide ()- and ()-Flutriafol.

Flutriafol, a globally utilized triazole fungicide in agriculture, is typically applied as a racemic mixture, but its enantiomers differ in bioactivity and environmental impact. The synthesis of flutriafol enantiomers is critically dependent on chiral precursors: 2,2-bisaryl-substituted oxirane [(2-fluorophenyl)-2-(4-fluorophenyl)oxirane, ] and 1,2-diol [1-(2-fluorophenyl)-1-(4-fluorophenyl)ethane-1,2-diol, ]. Here, we engineered a epoxide hydrolase (EH), obtaining mutant / with a 6.

Bioinformatics Analysis of the Expression and Prognostic Value of PLAU Gene in Wilms' Tumor.

Background/aim: Autophagy and immunity play important roles in the growth of malignant tumors and are promising targets for tumor therapy. This study was conducted to identify differentially expressed immune genes related to autophagy in Wilms' tumor (WT) and analyze their correlation with the disease prognosis.

Materials And Methods: The public data of WT and normal kidney tissues were downloaded from TCGA, ImmPort, and GeneCards databases to obtain differentially expressed immune genes associated with autophagy.

Identification and analysis of epithelial-mesenchymal transition-related key long non-coding RNAs in hypospadias.

EMT dysfunction is a dominant mechanisms of hypospadias. Thus, identification of EMT-related lncRNAs based on transcriptome sequencing data of hypospadias might provide novel molecular markers and therapeutic targets for hypospadias. First, the microarray data related to hypospadias were downloaded from Gene Expression Omnibus (GEO).

Sulfur-regulated CoSe nanowires with high-charge active centers for electrochemical nitrate reduction to ammonium.

Developing high-efficiency electrocatalysts for nitrate-to-ammonia transformation holds significant promise for the production of ammonia, a crucial component in agricultural fertilizers and as a carbon-free energy carrier. In this study, we propose a viable strategy involving sulfur doping to modulate both the microstructure and electronic properties of CoSe for nitrate reduction. This approach remarkably enhances the conversion of nitrate to ammonia by effectively regulating the adsorption capability of nitrogenous intermediates.

Solidophobic Surface for Electrochemical Extraction of High-Valued Mg(OH) Coupled with H Production from Seawater.

A significant challenge in direct seawater electrolysis is the rapid deactivation of the cathode due to the large scaling of Mg(OH). Herein, we synthesized a Pt-coated highly disordered NiCu alloy (Pt-NiCu alloy) electrode with superior solidophobic behavior, enabling stable hydrogen generation (100 mA cm, >1000 h durability) and simultaneous production of Mg(OH) (>99.0% purity) in electrolyte enriched with Mg and Ca.