Host Shaping Associated Microbiota in Hydrothermal Vent Snails from the Indian Ocean Ridge.

Snails at hydrothermal vents rely on symbiotic bacteria for nutrition; however, the specifics of these associations in adapting to such extreme environments remain underexplored. This study investigated the community structure and metabolic potential of bacteria associated with two Indian Ocean vent snails, and . Using microscopic, phylogenetic, and metagenomic analyses, this study examines bacterial communities inhabiting the foot and gland tissues of these snails.

Biological detoxification and application of emerging control strategies of zearalenone in food.

Zearalenone (ZEA) is a mycotoxin produced by filamentous fungi of the Ascomycota phylum, widely present in grains such as corn and wheat. The global contamination of ZEA is becoming increasingly severe, with a significant rise in its detection rate in grains and animal feed, posing serious threats to the health of livestock and poultry, the safety of processed grain products, and human health. This paper reviews the residual risks of ZEA, particularly its significant effects on the reproductive system and liver.

An Interpretable Radiomics Model Integrating Ultrasound and Clinical Features for Multiclass Classification of Axillary Lymph Nodes.

Objective: Accurate identification of common lesions (benign, metastatic carcinoma, lymphoma) of axillary lymph nodes (ALNs) is of vital importance for clinical decision-making, directly affecting tumor staging, treatment strategy formulation and prognosis of patients. However, current diagnostic methods by radiologists have limited accuracy in differentiating between benign lesions, metastatic carcinoma, and lymphoma.This study aimed to develop and validate the Axillary Lymph Node Interpretable Prediction (ALNIP) model for classifying axillary lymph nodes into benign, metastatic carcinoma, or lymphoma based on ultrasound imaging.

Apigenin mitigates intestinal barrier dysfunction in sepsis by modulating the AKT signaling pathway.

Sepsis is a systemic inflammatory response syndrome triggered by infection, which can lead to multiple organ dysfunction. This study untangles the synergistic multi-mechanistic effects of the natural flavonoid apigenin in ameliorating this pathological process. Utilizing a murine sepsis model and Caco-2 cell line, we systematically investigated the impact of apigenin on intestinal barrier function.

Integration of multi-omics approaches in exploring intra-tumoral heterogeneity.

Intra-tumoral heterogeneity (ITH) is common in malignant tumors and arises from dynamic variations across genetic, epigenetic, transcriptomic, proteomic, metabolic, and microenvironmental factors. This complexity drives tumor evolution and treatment resistance, undermining the accuracy of clinical diagnosis, prognosis, and treatment planning. Despite recent advances in multi-omics technologies that enable comprehensive mapping of ITH across molecular layers, major challenges remain in clinical translation.

Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosis.

Clathrin-mediated endocytosis internalizes proteins and lipids from the cell surface. A flexible condensate of initiator proteins catalyzes assembly of clathrin-coated vesicles in diverse organisms. Here we reveal that an endocytic adaptor protein, Epsin1, conditionally stabilizes this network, creating a cargo-dependent endocytic checkpoint.

Single-Cell Profiling of Peripheral and Intrahepatic B Cells Reveals Altered Responses and a CCR7⁺ Subset Linked to Antiviral Potential in Chronic HBV Infection.

B cell is a crucial component of adaptive immunity that plays a paramount role in the progression and prognosis of chronic hepatitis B virus (HBV) infection. However, detailed and systematic overviews of B cells are lacking, hindering their clinical applications in fighting against HBV. This study aims to provide a landscape of B cell responses in the context of chronic HBV infection.

Differences in Diversity of Collembola Communities Between Primary and Secondary Forests and Driving Factors.

Primary forests harbor extraordinary biodiversity, but conversion from primary forests to secondary forests often leads to biodiversity loss and diminished ecosystem functioning. While much of the existing research has focused on plants and vertebrates, soil fauna-particularly Collembola-remain underexplored in this context. To address this gap, we conducted a comprehensive assessment of the Collembola diversity and community composition in primary and secondary forests across two regions in northeastern China.

Correction: Liu et al. GCN5 Potentiates Glioma Proliferation and Invasion via STAT3 and AKT Signaling Pathways. 2015, , 21897-21910.

In the original publication [...

Effect of sevoflurane-based or propofol-based anesthesia on acute kidney injury after surgery for gastric cancer: a retrospective propensity score-matched analysis.

Background: Acute kidney injury (AKI) is a common complication of major abdominal surgery that is associated with adverse patient outcomes including death. The objective of this study was to investigate the incidence of postoperative AKI after gastric cancer surgery, comparing patients who received propofol-based TIVA with those who received sevoflurane-based INHA.

Methods: We analyzed the medical records of all patients aged 19 years or older who underwent radical surgery for primary gastric cancer at the Harbin Medical University Cancer Hospital between January 2010 and September 2018.

A natural ultrasound-triggered nitric oxide booster for endothelial dysfunction therapy.

Vascular endothelial dysfunction constitutes a pivotal initiating event in atherosclerosis (AS) pathogenesis, characterized by upregulated inflammatory factors, elevated reactive oxygen species (ROS) production, and excessive nitric oxide (NO) depletion. The exogenous delivery of NO, while minimizing adverse effects on the human body, presents a potent strategy for maintaining vascular homeostasis during pathological events. Herein, a natural ultrasound (US)-triggered and "on-demand" NO booster (FPG) is constructed using -nitrosoglutathione (GSNO) as the NO donor and fucoidan for its anti-inflammatory and antioxidant properties.

Phosphorylation-dependent association of a bacterial effector with plant 14-3-3 proteins enhances effector stability and bacterial virulence.

Gram-negative bacterial pathogens inject effector proteins inside plant cells using specialized secretion systems. These effectors manipulate plant cellular functions and suppress the plant immune system in order to promote bacterial proliferation. Although bacterial effectors are exogenous proteins that could be targeted by protein degradation systems within plant cells, they are able to perform their virulence functions.

Beat-to-Beat Blood Pressure Monitoring and Orthostatic Hypotension-Related Falls in Two Cohorts of Older Adults.

Falls are a major public health issue among older adults, often related to postural or orthostatic hypotension (OH). The optimal timing and methods for measuring blood pressure (BP) to assess OH and its relationship with falls are uncertain. We analyzed data from two older cohorts: the PROHEALTH study ( = 30, aged ≥ 65 years) and the NILVAD-CBF trial ( = 58, aged ≥ 50 years).

The gut microbiome: a vital link to hyperuricemia, gout and acute flares?

Objectives: To explore the associations between the gut microbiome and asymptomatic hyperuricemia, as well as acute gout flares.

Methods: Forty-three Chinese participants were divided into healthy and hyperuricemic groups according to serum uric acid (SUA) levels. The hyperuricemia group were further separated into asymptomatic hyperuricemia (HUA) and gout patients on the basis of their clinical symptoms.

Interaction of high pressure-stressed soy protein isolate and 5-methyltetrahydrofolate and its impact on the stability of 5-methyltetrahydrofolate.

5-Methyltetrahydrofolate (5-MTHF) is considered as a safer fortifier than folic acid (FA), but still unstable and less bioavailable. To investigated the soy protein isolate (SPI) or high hydrostatic pressure (HHP) treated SPI on the thermal stability of 5-MTHF and interaction of protein with 5-MTHF, the thermal stability of 5-MTHF in SPI-5MTHF complexes and HHP treated SPI-5MTHF complex was firstly investigated, and then revealed the effect of HHP on the structure and micromorphology of SPI by circular dichroism spectroscopy, free sulfhydryl content and scanning electron microscopy. Finally, the effect of HHP on the ability of the SPI to bind to 5-MTHF was also investigated by fluorescence spectrometry and isothermal titration.

Stimuli-responsive nanoparticles with tailorable elastin-like polypeptide chains for improved brain tumor targeting and therapy.

Glioblastoma (GBM), the most aggressive brain tumor, poses significant therapeutic challenges due to its infiltrative growth, resistance to conventional therapies, and the impermeable blood-brain barrier (BBB), which restricts drug delivery. To address these limitations, we developed a stimuli-responsive nanoplatform based on elastin-like polypeptides (ELPs)-biocompatible, thermally responsive biopolymers that enable precise drug release. The length-variable ELPs endow nanoparticles with tunable physicochemical characteristics, enabling the optimization of delivery efficiency through length-specific selection.

Efficient extracellular expression and rapid screening of human-like recombinant gelatin in Komagataella phaffii.

To obtain a strain that efficiently secretes and expresses hlrGEL (recombinant human-like gelatin), and to establish a rapid method for detecting this expression, this study began with the original strain GS115/pPICZα-gel6 (4# clone). High-copy transformants were obtained through Zeocin resistance screening, and positive expression strains were rapidly screened using single-cell laser Raman spectroscopy (SCLRS) technology. The results revealed that the Raman spectral features of positive expressing strains were located at 1447 cm, 1658 cm, and 2929 ~ 2943 cm, and their peak intensities were correlated with the expression level of hlrGEL while being consistent with SDS-PAGE, Western blotting and BCA methods analysis results.

Lateral Coordination Sulfur-Modified Charge Asymmetry Cu-Ru Diatomic Catalyst for Efficient and Stable Electrochemical Nitrate Reduction to Ammonia.

Double-atom catalysts (DACs) with asymmetric coordination are crucial for enhancing the benefits of electrochemical reduction of nitrate (NO) to ammonia (NH) and advancing sustainable development; however, the rational design of DACs is still challenging. In this study, we synthesized atomically dispersed catalysts with lateral coordination sulfur-modified Cu-Ru sites (named NSCu-RuN/SNC). The abundant defects and low-electronegativity heteroatoms in the carbon-based framework endow the asymmetric local structure of NSCu-RuN at the atomic level, which is confirmed by aberration-corrected electron microscopy and X-ray absorption spectroscopy (XAS).

Chimeric collagen-like proteins with tunable structural heterogeneity for precise control and targeted doxorubicin delivery.

Protein-based drug delivery systems offer advantages such as genetic tunability, structural homogeneity, and high biocompatibility. However, precisely controlling the assembly of protein nanoparticles and establishing correlations between their macroscopic properties and molecular architecture remain significant challenges. Here, inspired by chimeric collagen-like proteins, we rationally designed a series of de novo chimeric proteins comprising an N-terminal globular domain fused to a linear collagen-like domain.

The impact mechanism of media use on the green and low carbon consumption behavior of China: integrating planned behavior theory and social cognitive theory.

As a crucial medium for information dissemination and public opinion guidance, how to leverage media to promote the development of public green and low-carbon consumption (GLC) is a major challenge in the new era. This study, based on the Theory of Planned Behavior and Social Cognitive Theory, employs PLS-SEM and MGA methods to construct a model encompassing traditional media, online media, and social media channels, delving into the relationship between media use (MU) and GLC. The findings indicate that media usage is positively correlated with both aspects of GLC: green and low-carbon consumption intention, green and low-carbon consumption behavior.

Multi-omics characterization of diabetic nephropathy in the db/db mouse model of type 2 diabetes.

Background: Despite optimized blood pressure control and glycemic management reducing the incidence of diabetic nephropathy (DN), significant residual risk remains, suggesting the contribution of pathogenic factors independent of glucose metabolism and hemodynamic disturbances.

Methods: Renal tissues from db/db mice underwent integrative multi-omics analysis, encompassing transcriptomics, metabolomics, and lipidomics. Orthogonal projection to latent structures-discriminant analysis (OPLS-DA) was applied to identify significant metabolic perturbations, while bidirectional O2PLS integration elucidated metabolic-transcriptomic correlations.

Detection of EEG dynamic complex patterns in disorders of consciousness.

Diagnosing Disorders of Consciousness (DoC) remains a critical challenge in cognitive neuroscience. In this study we introduce Electroencephalography (EEG)-based brain states as a real-time, bedside tool for assessing dynamic brain connectivity in DoC patients. We analyze EEG data from 237 acute and chronic DoC patients across three centers, identifying five recurrent functional connectivity patterns.

Remote ischaemic conditioning improves outcomes of ischaemic stroke treated by endovascular thrombectomy: the SERIC-EVT trial.

Background And Aims: Even after endovascular thrombectomy, more than half of patients with acute large vessel occlusion stroke do not achieve favourable outcomes. This study aimed to assess the efficacy and safety of remote ischaemic conditioning (RIC), a promising neuroprotective treatment, in patients with acute ischaemic stroke who received endovascular thrombectomy.

Methods: This participant-blinded, randomized controlled clinical trial was conducted at 25 hospitals.

Design and analysis of a 0.36 NA central obscuration extreme ultraviolet objective system.

The prevalent 0.33 NA unobstructed extreme ultraviolet lithography (EUVL) six-mirror objective system is characterized by a large average incidence angle at M5, which adversely affects imaging performance and energy transmission following coating. In order to improve the system performance of coated EUVL objectives, this paper presents an innovative 0.

Prediction of breast cancer HER2 status changes based on ultrasound radiomics attention network.

Background And Objective: Following Neoadjuvant Chemotherapy (NAC), there exists a probability of changes occurring in the Human Epidermal Growth Factor Receptor 2 (HER2) status. If these changes are not promptly addressed, it could hinder the timely adjustment of treatment plans, thereby affecting the optimal management of breast cancer. Consequently, the accurate prediction of HER2 status changes holds significant clinical value, underscoring the need for a model capable of precisely forecasting these alterations.