A magnetic flower-like hollow vinyl-functionalized covalent organic framework nanocomposite for efficient enrichment of fifteen organophosphate tri-esters in environmental water samples.

Background: Organophosphate tri-esters (tri-OPEs), widely used flame retardants, include alkyl-, halogenated-, and aryl-substituted types with distinct physicochemical properties. They may readily enter the ambient environment through volatilization, mechanical abrasion, and dissolution mechanisms occurring throughout the product lifecycle. To date, a range of monitoring methodologies incorporating sample pretreatment techniques have been developed to characterize the environmental distribution of tri-OPEs.

TRIM6 ablation reverses ICB resistance in MSS gastric cancer by unleashing cGAS-STING-dependent antitumor immunity.

Background: Gastric cancers are classified into four molecular subtypes according to The Cancer Genome Atlas (TCGA) classification: Epstein-Barr virus-positive (EBV-positive), microsatellite instability-high (MSI-H), chromosomal instability (CIN), and genomically stable (GS). Unlike MSI-H gastric cancer, GS and CIN subtypes exhibit immunologically inert microenvironments and demonstrate minimal response to immune checkpoint blockade (ICB), necessitating novel strategies to overcome immunotherapy resistance.

Methods: Through weighted gene co-expression network analysis (WGCNA), we identified the E3 ubiquitin ligase TRIM6 as inversely associated with MSI-H status.

Integrating 7-day D-dimer exposure into deep vein thrombosis risk prediction after gastrointestinal surgery.

Deep vein thrombosis (DVT) is a serious complication following gastrointestinal surgery. While D-dimer is a widely used biomarker for thrombosis, its postoperative specificity is limited due to inflammatory interference. This study introduces a novel cumulative metric-7-day D-dimer exposure (7dDDE)-to quantify perioperative coagulation burden.

USP3 stabilizes MIC19 by deubiquitination under hypoxic stress and promotes the progression of non-small cell lung cancer.

Hypoxia is a common phenomenon in the microenvironment of solid tumors; mitochondria, as the site of cellular oxidative respiration, are among the first organelles to be affected under hypoxic conditions. Mitochondrial cristae organizing protein 19 (MIC19), a core component of the mitochondrial contact site and cristae organizing system (MICOS), is essential for preserving mitochondrial activity. In this study, we investigated the effects of hypoxia on MIC19 and its regulatory mechanisms in non-small cell lung cancer (NSCLC).

Risk Assessment of Precancers and Cancers in Women with Atypical Glandular Cells of Endocervical, Endometrial, and Unknown Origin.

To assess the risk of precancers [high-grade squamous intraepithelial lesions (HSIL), adenocarcinoma (AIS) and atypical endometrial hyperplasia (AEH)] and cancers [squamous cell carcinoma (SCC) and adenocarcinoma (ADC)] in women with atypical glandular cells (AGC) cytology. : A total of 1,028 women diagnosed with abnormal cervical glandular cytology from January 2019 and December 2023 were enrolled. Of these, 670 underwent both HPV genotyping and cervical biopsy.

Assessing the Protective Role of Cheese Consumption Against Type 2 Diabetes and Its Complications: A Mendelian Randomization Study.

Type 2 diabetes mellitus (T2DM) is a major global health issue, with significant complications impacting patients' quality of life, including neuropathy, ophthalmic issues, nephropathy, and peripheral vascular complications. Although dietary factors influence T2DM risk, the specific impact of cheese consumption remains unclear. This study uses a two-sample Mendelian randomization (MR) approach to investigate the causal relationship between cheese intake and T2DM, along with specific complications, including ophthalmic and peripheral vascular issues.

Elucidating the mechanism of triphenyl phosphate interference in bone metabolism via network toxicology and molecular docking methodologies.

Objective: This study aims to elucidate the molecular mechanisms by which the widely used organophosphate flame retardant and plasticizer, triphenyl phosphate (TPhP), disrupts bone metabolism, highlighting the potential impact of environmental chemicals on bone homeostasis.

Methods: A combined approach of network toxicology and molecular docking was employed to investigate the molecular mechanisms underlying the effects of TPhP on bone metabolism. Potential targets associated with both TPhP and bone metabolism were identified through database searches in ChEMBL, STITCH, GeneCards, and OMIM.

Design of Integral-Based HOSM Controller Under Perturbations of Unknown Magnitudes.

This article is committed to the establishment of an integral-based high-order sliding mode (iHOSM) controller for one category of nonlinear systems when confronted with perturbations with unknown magnitudes. A striking characteristic is that an integral dynamics is deftly constructed and then incorporated into the original sliding mode system to dispose of the perturbations of unknown magnitudes. On account of the new sliding mode dynamics, we explicitly introduce a systematic design protocol to dexterously design a new iHOSM controller.

Application and mechanisms of targeting BRD4 in osteosarcoma.

Objectives: Metastasis is the primary cause of death in osteosarcoma, and current clinical treatments remain limited. BRD4, a key epigenetic regulator, has shown therapeutic promise in various cancers through its inhibition. However, the mechanistic role of BRD4 in osteosarcoma remains poorly understood.

enhances growth and salt tolerance in a short rotation woody crop, , under NaCl stress.

Salinization is a major environmental challenge that jeopardizes productivity and resilience of plants such as the short rotation woody crops (SRWC) and bioenergy crops. Leveraging beneficial microbes will enhance plant resistance to salinity with physiological adjustments. Here we investigated the efficacy of plant growth promoting fungus () on optimizing growth and salt tolerance of SRWCs and bioenergy tree crops, using as an example.

Cancer biomarkers discovered using pan-cancer plasma proteomic profiling.

Mass-spectrometry-based proteomic data of tumour patient plasma samples present opportunities for improving cancer detection. Here we generate plasma proteomic profiles from 2,251 pan-cancer patient samples and investigate potential diagnostic biomarkers. Proteomic subtyping with different dominant tumour types links proteomic features and clinical indicators such as tumour stage.

Prognostic and Therapeutic Value of Metabolism-Related Genes in Nephroblastoma: A Focus on the Key Gene NNMT and Its Regulative Effect on Metabolism.

Metabolic reprogramming plays an essential role in the initiation and aggressiveness of malignant tumors. This study aims to establish a prognostic signature for Wilms tumor (WT) based on metabolism-related genes (MRGs) and to explore potential molecular mechanisms. RNA sequencing data of WT samples and MRGs were sourced from GEO, TCGA and KEGG, respectively.

Changes in meat consumption can improve groundwater quality.

Large-scale, centralized livestock production is recognized as a significant contributor to environmental pollution, including groundwater contamination. Here we assess the impact of traditional meat production on nitrate contamination in groundwater across the contiguous USA from 1985 to 2020. In addition, we evaluate potential changes in groundwater quality resulting from the substitution of traditional meat sources with three alternative meat options.

Proteogenomic characterization reveals that lipid droplet formation promotes esophageal squamous cell cancer progression.

Esophageal cancer is one of the most common cancers and a leading cause of cancer-related mortality in the world. Here, we have performed comprehensive characterization of genetic, transcriptomic, proteomic, and phosphoproteomic features in 293 patients with esophageal squamous cell carcinoma (ESCC). Chromosome 12q13.

Targeting lactylation reinforces NK cell cytotoxicity within the tumor microenvironment.

Dysfunction of natural killer (NK) cells can be associated with tumor-derived lactate in the tumor microenvironment. Lactate-induced lysine lactylation (Kla) is a posttranslational modification, and strategies aimed at augmenting NK cell resistance to Kla might enhance cytotoxicity. Here we show that increased Kla levels in NK cells are accompanied by impaired nicotinamide adenine dinucleotide metabolism, fragmented mitochondria and reduced cytotoxicity.

Ionotropic receptors participate in the perception of sinigrin in Plutella xylostella adults.

Glucosinolates serve as the key gustatory cue for crucifer specialists to select their host plants, however, the molecular mechanisms underlying the gustation remain poorly understood. Here, we demonstrated that two ionotropic receptor co-receptors (Ircos) were involved in the perception of a major glucosinolate, sinigrin, in the adult Plutella xylostella. First, we cloned 13 gustatory receptors (GRs) expressed in adults (antennae and tarsi) and larvae (heads), and analyzed their functions using the Xenopus oocyte expression system.

Screening and Identification of Basement Membrane-Related Gene Signatures for Diagnosis in Keratoconus Through WGCNA and Machine Learning.

Keratoconus (KC) can lead to severe vision loss, impacting daily life. The etiology of KC is not yet clear, and early diagnosis and treatment are crucial for prognosis. This study aimed to explore basement membrane (BM)-related gene signatures for the diagnosis and therapy of KC and provide novel insights into its pathogenesis.

Long-term results of cervical disc arthroplasty: a single-center retrospective study with a minimum 10-year follow-up.

Objective: This study aimed to retrospectively evaluate the long-term clinical and radiological outcomes following cervical disc arthroplasty (CDA).

Methods: This study included 74 patients who underwent single- or two-level CDA between November 2004 and December 2013, with a minimum 10-year follow-up (22 in the Bryan disc group and 52 in the Prestige LP disc group). Patient-reported outcomes and radiological parameters were collected for comparisons.

AI in fungal drug development: opportunities, challenges, and future outlook.

The application of artificial intelligence (AI) in fungal drug development offers innovative strategies to address the escalating threat of fungal infections and the challenge of antifungal resistance. This review evaluates the current landscape of fungal infections, highlights the limitations of existing antifungal therapies, and examines the transformative potential of AI in drug discovery and development. We specifically focus on how AI can enhance the identification of new antifungal agents and improve therapeutic strategies.

Prenatal supplementation with the gut-derived tryptophan metabolite indole-3-propionic acid alleviates colitis susceptibility in maternal immune-activated offspring mice.

Introduction: Maternal immune activation (MIA) impairs gut immune function in offspring, with maternal microbiota and their metabolites influencing intestinal development. Indole-3-propionic acid (IPA), a microbial metabolite derived from tryptophan, promotes gut health by enhancing epithelial proliferation. However, the impact of prenatal IPA supplementation on offspring gut outcomes remains unclear.

Integrated plasma and vegetation proteomic characterization of infective endocarditis for early diagnosis and treatment.

Infective endocarditis, a life-threatening condition, poses challenges for early diagnosis and personalized treatment due to insufficient biomarkers and limited understanding of its pathophysiology. Here, we performed proteomic profiling of plasma and vegetation samples from 238 patients with infective endocarditis and 100 controls, with validation in two external plasma cohorts (n = 328). We developed machine learning-based diagnostic and prognostic models for infective endocarditis, with area under the curve values of 0.

Integrated clinical and proteomic-based model for diagnostic and prognostic prediction in pRCC.

Papillary renal cell carcinoma (pRCC), a main pathological subtype of non-clear cell RCC (nccRCC), has strong heterogeneity. Comparing to other nccRCC subtypes, advanced pRCC has the poorest prognosis. Due to its lower incidence compared to ccRCC, clinical research and exploration of non-invasive biomarkers for pRCC are limited, and it is often misclassified.