SOBIR1 Links Potato Responses to Potato Tuber Moth Attack and High Temperature Stress.

Herbivory and high temperature stress affect plant performance and frequently co-occur under natural conditions. The molecular mechanisms by which plants coordinate responses to these two stresses deserve more attention. Here, we explored how StSOBIR1, a leucine-rich repeat receptor-like kinase gene, modulates plant responses to herbivory and high temperature stress using genetic, molecular, biological, and chemical analysis approaches.

Effect of aquaculture water temperature on the physicochemical and functional properties of fish scale gelatins.

The effects of culture temperatures on the preparation and properties of fish gelatins remain unclear. Herein, three gelatins (TScG: Tropical tilapia scale gelatin. GScG: Warm-water grass carp scale gelatin.

Enzymatic hydrolysis combined with chemiluminescence immunoassay: Foresight for automated and rapid urinary aldosterone detection.

Background: The 24-hour urinary aldosterone test offers superior stability in assessing aldosterone secretion compared to single-point plasma measurements, suggesting its potential utility as a diagnostic tool for primary aldosteronism. However, its clinical utility is hampered by labor-intensive manual urine treatment and the lack of diagnostic cut-off, with the former demonstrating considerable inter-laboratory variability. There is an urgent need for an automated assay to facilitate rapid and standardized 24-hour urinary aldosterone testing, accompanied by method-specific diagnostic cut-offs.

Unveiling the UHR threshold in American adult: a new biomarker for early diabetes detection from NHANES study.

Background And Aims: This study examines the association between the serum uric acid high-density lipoprotein cholesterol ratio (UHR) biomarker and the risk of prediabetes and diabetes, a relationship that remains under-researched, to provide insights for diabetes prevention.

Methods And Results: This study utilized data from 13,975 American participants in the National Health and Nutrition Examination Survey (2011-2018). Logistic regression models, restricted cubic splines (RCS) analysis and subgroup analyses were employed to examine the association between UHR and prediabetes/diabetes.

Alpine radish rhizosphere microbiome assembly and metabolic adaptation under PBAT/PLA humic acid biodegradable mulch films.

Introduction: Alpine agroecosystems present unique crop production challenges due to extreme environmental conditions, where rhizosphere microbiomes significantly influence plant adaptation.

Methods: To investigate mulch-induced microbial changes in high-altitude agriculture, this study analyzed a radish field in China using SMRT sequencing (16S rRNA/ITS) and metagenomics, comparing PBAT/PLA biodegradable films with/without humic acid (HA) at varying thicknesses.

Results: Results demonstrated that radish cultivation selectively enriched and while depleting and , with fungal communities shifting from -to -dominance.

Translating a Preclinically Tested 15 Hz rTMS Protocol to Humans With Chronic Spinal Cord Injury: A Safety and Feasibility Study.

Objectives: Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive brain stimulation strategy with a demonstrated potential to reinforce the residual pathways after a spinal cord injury (SCI). A preclinically tested high-frequency (15 Hz) rTMS (15 Hz rTMS) protocol was shown to induce corticospinal tract axon regeneration growth and sprouting, resulting in improved voluntary motor control and performance. In a translational perspective, we aimed to investigate the safety and feasibility of the 15 Hz rTMS paradigm as an adjunct therapeutic intervention in individuals with chronic SCI.

Preparation and characterization of low-phenylalanine polypeptides from whey protein.

Low-Phe protein-based substances are an important clinical requirement for the development of medical foods. Herein, a combination method of two-step enzymatic hydrolysis with activated carbon adsorption removal was applied to prepare low-Phe whey protein polypeptides (LWPs). The two-step enzymatic hydrolysis was affected by enzyme/casein mass ratios and times.

Dietary Choline Intake and Chronic Kidney Disease: Evidence from NHANES 2005 to 2020.

Objective: Chronic kidney disease (CKD) is a prevalent condition with substantial morbidity and mortality, and dietary and nutritional factors often play a key role in its progression. Choline, an essential nutrient, has been linked to various health outcomes, including cardiovascular diseases and nonalcoholic fatty liver disease. However, the association between dietary choline intake and CKD remains unclear.

MRLF-DDI: A Multi-view Representation Learning Framework for Drug-Drug Interaction Event Prediction.

Accurately predicting drug-drug interaction events (DDIEs) is critical for improving medication safety and guiding clinical decision-making. However, existing graph neural network (GNN)-based methods often struggle to effectively integrate multi-view features and generalize to novel or understudied drugs. To address these limitations, we propose MRLF-DDI, a multi-view representation learning framework that jointly models information from individual drug features, local interaction contexts, and global interaction patterns.

Cognitive Improvement in Children With Cerebral Palsy Following Combined Scalp Acupuncture and High-Frequency Repetitive Transcranial Magnetic Stimulation (rTMS): A Prospective Observational Study.

Objective:  To evaluate the clinical efficacy of combining scalp acupuncture and high-frequency repetitive transcranial magnetic stimulation (rTMS) for improving cognitive function in children with cerebral palsy (CP) and cognitive impairment.  Methods: In this prospective observational study, 60 children with CP and cognitive impairment were recruited from January 2023 to June 2024 at a tertiary rehabilitation center. Participants were divided into two groups (n=30 each): the observation group received scalp acupuncture plus high-frequency rTMS (3 sessions/week), while the control group received scalp acupuncture alone (same frequency).

A novel immunoprecipitation-based targeted liquid chromatography-tandem mass spectrometry analysis for accurate determination for copeptin in human serum.

Objectives: Copeptin, a stable and simple-to-measure surrogate marker for arginine vasopressin (AVP), demonstrates excellent clinical values, particularly in diagnosing polyuria-polydipsia syndromes. However, conventional immunoassay methods are limited and lack comparability. Our aim was to establish a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying copeptin in human serum.

Development of an interpretable machine learning model for frailty risk prediction in older adult care institutions: a mixed-methods, cross-sectional study in China.

Objective: To develop and validate an interpretable machine learning (ML)-based frailty risk prediction model that combines real-time health data with validated scale assessments for enhanced decision-making and targeted health management in integrated medical and older adult care institutions (IMOACIs) in central China.

Design: Mixed-methods, cross-sectional study.

Setting: 13 IMOACIs across seven cities in Hunan province, central China, from 8 to 16 July 2022.

Adipose-derived mesenchymal stem cell-derived extracellular vesicles carry microRNA-214-3p to target GSTZ1 to curb ferroptosis in lung epithelial cells during sepsis.

Ferroptosis pitches in sepsis-caused pulmonary diseases. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) pitches in ferroptosis. This study explored the mechanism of adipose-derived MSC-EVs (ADMSC-EVs) protecting against ferroptosis in lung epithelial cells during sepsis.

Simplification of the oral sodium loading test: 2-day method replacing 3-day method.

Objectives: As one of the diagnostic tests for primary aldosteronism (PA), the oral sodium loading test (OSLT) usually takes 3 days, which is time-consuming.This study aims to investigate the possibility of the 2-day method replacing the 3-day method in the OSLT.

Methods: In this prospective study, both PA patients and healthy participants were recruited and underwent an oral high-sodium diet for 3 consecutive days from December 2023 to December 2024.

[Neuroprotective Effects of Anisodine Hydromide in a Rat Model of Vascular Dementia and the Antioxidative Stress Mechanisms Involved].

Objective: Vascular dementia (VD) is a common cognitive dysfunction associated with cerebrovascular disease. This study is aimed at investigating the therapeutic effect of anisodine hydromide (AH) on VD and the potential antioxidative stress mechanisms involved.

Methods: A VD model was established in Sprague-Dawley (SD) rats through permanent bilateral common carotid artery occlusion.

Neuropathic Pain-Like Responses in a Chronic CNS Injury Model Are Mediated by Corticospinal-Targeted Spinal Interneurons.

Chronic neuropathic pain is a persistent and debilitating outcome of traumatic central nervous system injury, affecting up to 80% of individuals. Postinjury pain is refractory to treatments due to the limited understanding of the brain-spinal cord circuits that underlie pain signal processing. The corticospinal tract (CST) plays critical roles in sensory modulation during skilled movements and tactile sensation; however, a direct role for the CST in injury-associated neuropathic pain is unclear.

AI-driven discovery of brain-penetrant Galectin-3 inhibitors for Alzheimer's disease therapy.

Galectin-3 (Gal-3) has emerged as a critical regulator of neuroinflammation and a promising therapeutic target for Alzheimer's disease (AD). Nevertheless, the development of brain-penetrant small-molecule Gal-3 inhibitors poses a significant challenge. To address this, we employed an artificial intelligence (AI)-driven drug discovery platform, identifying FJMU1887 as a novel Gal-3 inhibitor possessing optimized pharmacokinetic properties and favorable blood-brain barrier (BBB) permeability.

PTGDS deficiency as a driver of M2 macrophage polarization and immunosuppressive microenvironment in esophageal squamous cell carcinoma: an experimental study.

Introduction: Esophageal squamous cell carcinoma (ESCC) exhibits poor survival linked to M2 macrophage-polarized microenvironment. This study aimed to elucidate the role of prostaglandin D2 synthase (PTGDS) in modulating tumor microenvironment (TME) and its prognostic implications in ESCC.

Materials And Methods: Transcriptomic datasets (GSE53624, GSE121931) were analyzed using immune deconvolution and weighted gene co-expression network analysis to identify genes associated with M2 macrophage infiltration.

Cascade Electrocatalytic Reduction of Nitrate to Ammonia Using Bimetallic Covalent Organic Frameworks with Tandem Active Sites.

Electrochemical nitrate reduction reaction (NORR) is a promising approach to simultaneously realize pollutant removal and ammonia generation. However, this process involves the transfer of eight electrons and nine protons along with multiple by-products, resulting in a significant challenge for achieving high ammonia yield and selectivity. Herein, we introduced bimetallic covalent organic frameworks catalysts with Cu and Co active sites to achieve a two-step tandem reaction, avoiding excessive nitrite accumulation and enabling efficient NORR.

Yishen-Huoxue formula alleviates renal interstitial fibrosis by attenuating hypoxia-induced renal cell injury and promoting angiogenesis via miR-210/HIF-1α pathway.

Background: Renal interstitial fibrosis (RIF) represents the final common pathway in nearly all progressive chronic kidney diseases. This study aimed to investigate the effects of Yishen-Huoxue formula (YHF) on RIF and its underlying mechanisms.

Methods: Unilateral ureteral obstruction (UUO) model was applied to induce RIF .

A deep learning-based method for predicting the frequency classes of drug side effects based on multi-source similarity fusion.

Motivation: Drug side effects refer to harmful or adverse reactions that occur during drug use, unrelated to the therapeutic purpose. A core issue in drug side effect prediction is determining the frequency of these drug side effects in the population, which can guide patient medication use and drug development. Many computational methods have been developed to predict the frequency of drug side effects as an alternative to clinical trials.

Controlled preparation of fish oil powders via complex coacervation and genipin crosslinking.

The application of fish oil is limited by its unpleasant fishy flavour, sensitivity to environments (e.g., oxygen, light, and heat), and poor water solubility.

Genome-wide identification and expression analyses of CYP450 genes in Chrysanthemum indicum.

Background: The cytochrome P450 superfamily comprises a large group of enzymes crucial for the biosynthesis and metabolism of diverse endogenous and exogenous secondary metabolites in plants. Chrysanthemum, an ornamental genus with considerable medicinal value, is one of the most economically important floricultural crops in the world. The characteristics and functions of CYP450 genes in Chrysanthemum species, however, remain largely unknown.

Comparison of the efficacy and safety of remimazolam and propofol for fiberoptic bronchoscopy in older patients: a prospective, randomized controlled study.

Objective: The aim of this study was to compare the efficacy and safety of remimazolam with those of propofol in older patients undergoing fiberoptic bronchoscopy with preserved spontaneous breathing.

Methods: Sixty older patients were randomly and equally divided into a remimazolam group (group R) and a propofol group (group P). Both groups received 0.