CCAAT-enhancer binding protein delta functions as a tumor suppressor gene in acute myeloid leukemia.

There is a continued need for identification of novel disease drivers of acute myeloid leukemia as many patients experience relapse and have poor clinical outcomes. Analyses from our study and publicly available datasets predicted CEBPD as a novel tumor suppressor gene in acute myeloid leukemia. Consistent with the analyses, CEBPD knockdown experiments showed activation of MAPK signaling with concomitant increase in cell growth rate, while upregulation experiments suggested induction of myeloid differentiation marker CD14 expression in AML cell lines OCI-AML2 and OCI-AML5.

Risk Assessment and Fertility Counseling for Hereditary Gynecological Cancer Syndromes.

Objective: To review the genetic basis, clinical characteristics, and management strategies of hereditary gynecologic cancers associated with hereditary cancer syndromes.

Methods: Literature on germline mutations, inheritance patterns, clinical manifestations, and fertility preservation strategies was reviewed.

Results: Germline pathogenic mutations, predominantly inherited in an autosomal dominant manner, increase susceptibility to gynecologic tumors with varying risks.

Zeaxanthin augments CD8 effector T cell function and immunotherapy efficacy.

The detailed mechanisms underlying the regulatory significance of dietary components in modulating anti-tumor immunity remain largely unknown. Here, we apply a co-culture-based screening approach using a blood nutrient compound library and identify zeaxanthin (ZEA), a dietary carotenoid pigment found in many fruits and vegetables and known for its role in eye health, as an immunomodulator that enhances the cytotoxicity of CD8 T cells against tumor cells. Oral supplementation with ZEA, but not its structural isomer lutein (LUT), enhances anti-tumor immunity in vivo.

Soyasaponin and vertical microbial transmission: Maternal effect on the intestinal development and health of early chicks.

Multiple factors, including genetics, nutrition, and health, influence the vertical transmission of microbiota from mothers to their offspring. Recent studies have shown that avian microbiota can be passed to the next generation via the eggshell and egg albumen. However, it remains unclear whether these microbial communities are regulated by nutrition and how they are associated with the host genotype.

Machine-Learning Models With Multiple Imputation With Sequential Nearest Neighbors Imputation for Predicting the Prognosis of Idiopathic Sudden Sensorineural Hearing Loss Patients.

Objectives: The prognosis of idiopathic sudden sensorineural hearing loss (ISSNHL) is heterogeneous. The study aimed to investigate the prognostic factors of ISSNHL and develop machine-learning models with multiple imputation to predict the prognosis of ISSNHL.

Design: A retrospective study was undertaken on a cohort of 600 patients with ISSNHL who underwent standardized treatment protocols.

A molecular dynamics protocol for rapid prediction of EGFR overactivation and its application to the rare mutations S768I, S768N, D761N.

Hyperactivation caused by mutations in the Epidermal Growth Factor Receptor (EGFR) kinase domain is implicated in various diseases, including cancer. However, the structural mechanisms underlying overactivation in many EGFR mutations remain poorly understood, and exploring these mechanisms through conventional experiments or simulations is often labor- and cost-intensive. Here, we establish a Molecular Dynamics (MD) protocol capable of rapidly revealing EGFR mutant modes of action using multiple short simulations.

Emerging roles of KLF4 regulation of autophagy in different physiological and pathological processes.

Krüppel-like factor 4 (KLF4) is a zinc finger transcription factor that exhibits both transcriptional activation and inhibition effects. It participates in the occurrence and development of various diseases by regulating processes such as cell cycle arrest, differentiation, and the maintenance of stem cell pluripotency. Autophagy, as a conserved lysosome dependent degradation pathway in eukaryotes, maintains cellular homeostasis by clearing abnormal proteins and damaged organelles.

Rapid identification of high-viscosity rice soup quality using neutral desorption extractive electrospray ionization mass spectrometry.

High-viscosity rice soup is not only a popular food item but also a traditional Chinese medicinal remedy. Herein, we developed a high-throughput characterization platform for high-viscosity rice soup analysis using Neutral Desorption Extractive Electrospray Ionization Mass Spectrometry (ND-EESI-MS) with untargeted metabolomics. The chemical fingerprint of high-viscosity rice soup was directly analyzed by ND-EESI-MS, with exogenous (, heavy metals and pesticides) and endogenous pollutants detected qualitative and quantitative tandem mass spectrometry.

Risk Factors for Postoperative Neurologic Dysfunction in Patients with Spinal Tuberculosis: A Retrospective Study.

Purpose: Unexplained neurological dysfunction often occurs in patients after spinal tuberculosis(STB) surgery; therefore, this study aimed to analyze the causes of this dysfunction from the perspectives of the patient's preoperative inflammatory state, carrier bacterial state, and increased degree of autoimmunity.

Patients And Methods: We collected 247 patients with STB and 270 patients with degenerative diseases of the spine admitted from May 2015 to December 2024 at the Second Hospital of Shanxi Medical University. According to the exclusion criteria, 132 patients for each disease were included in this study.

Dual-mode fluorescence and electrochemiluminescence sensor based on functionalized EDTA-HOFs for ultrasensitive detection of Hg.

Leveraging the unique properties of functionalized hydrogen-bonded organic frameworks (EDTA-HOFs), this study develops a dual-mode sensor platform-combining fluorescence and electrochemiluminescence-for the swift and sensitive detection of mercury ions (Hg⁺) in homogeneous solutions. We use tris(4,4'-dicarboxy-2, 2'-bipyridine)ruthenium(II) [Ru(dcbpy)₃⁺] as the signal emitter, introducing a heavy metal ion chelator (EDTA) into its structure and synthesizing the hydrogen-bonded organic framework compound EDTA-HOFs through intermolecular hydrogen bonding. EDTA-HOFs exhibit a large specific surface area and a robust hydrogen-bonding network, offering optimal N,N'-chelation sites for efficient metal ion recognition, thereby markedly improving the stability of both fluorescence (FL) and electrochemiluminescence (ECL) signals.

High-value utilization of volcanic rock as NH-SCR denitration catalyst loaded with Mn: denitration effect and mechanism.

Volcanic rock is a natural mineral with a porous structure and rich in various metal oxides. It has been used in environmental applications such as water and soil pollution remediation as stable chemical properties and low cost. In this work, volcanic rock was used in the field of nitrogen oxide control, and the feasibility of preparing NH- Selective Catalytic Reduction catalyst was evaluated.

Comprehensive analysis of 1,771 transcriptomes from 7 tissues enhance genetic and biological interpretations of maize complex traits.

By reanalyzing 1,771 RNA-seq datasets from 7 tissues in a maize diversity panel, we explored the landscape of multi-tissue transcriptome variation, evolution patterns of tissue-specific genes, and built a comprehensive multi-tissue gene regulation atlas to understand the genetic regulation of maize complex traits. Through an integrative analysis of tissue-specific gene regulatory variation with genome-wide association studies, we detected relevant tissue types and several candidate genes for a number of agronomic traits, including leaf during the day for the anthesis-silking interval, leaf during the day for kernel Zeinoxanthin level, and root for ear height, highlighting the potential contribution of tissue-specific gene expression to variation in agronomic traits. Using transcriptome-wide association and colocalization analysis, we associated tissue-specific expression variation of 74 genes to agronomic traits variation.

Gut microbiome dysbiosis accelerates osteoarthritis progression by inducing IFP-SM inflammation in "double-hit" mice.

Background: This study investigates the complex interplay between gut microbiome dysbiosis and systemic inflammation as a critical risk factor in the pathogenesis of osteoarthritis (OA). Furthermore, it elucidates the role of gut microbiota (GMB) dysbiosis in driving OA progression.

Methods: A refined "double-hit" murine model was developed to explore this relationship.

Supporting clinical reasoning through visual summarization and presentation of patient data: a systematic review.

Objectives: Clinicians retrieve data from electronic health record (EHR) systems and summarize them into clinical information to accomplish clinical reasoning and decision-making tasks. Visualization, using meaningful summarization methods and intuitive presentation approaches, can enhance this process. This systematic review examines how EHR data are summarized, visualized, and aligned with the 7 clinical reasoning and decision-making tasks shared by clinicians.

Effects of computer mouse control-display gain on upper extremity muscle fatigue, subjective fatigue and user performance.

BackgroundOperating a mouse for long periods of time increases the risk of muscle fatigue in the upper limbs of office workers.ObjectiveThis study aimed to contribute to the field of knowledge by providing guidance on the selection of a suitable computer mouse control-display gain (CDG) for office workers, and to reduce muscle fatigue and fatigue accumulation in office workers who use computer mice.MethodThirty healthy office workers used five different control-display gain settings to complete five sessions of a 30-min Fitts' task.

Cobalt Doping of NaVTi(PO) Enables a High-Energy NASICON-Type Cathode Material for Sodium-Ion Batteries.

Natrium superionic conductor (NASICON) compounds have emerged as a rising star in the field of sodium-ion batteries (SIBs) owing to their stable framework structure and high Na ionic conductivity. The NASICON-structured NaVTi(PO) manifests significant potential as Na storage material, characterized by decent rate capability and cyclability. However, the low redox potential of Ti/Ti and undesirable energy density limit its practical applications.

Development and in vitro characterization of humanized antibodies for blocking human TRPM4 channel.

Transient receptor potential melastatin 4 (TRPM4) channel, a monovalent cation channel, plays a crucial role in various neurological disorders. We previously showed that TRPM4 activity can be blocked by our antibodies: M4P, which targets rat TRPM4 and M4M, against a similar antigenic epitope of human TRPM4. M4P and M4M demonstrated efficacy in mitigating stroke reperfusion injury.

C-Axis Chiral 10,10'-Bibenzoquinoline Ligands Specifically Partnered with AgOTf Enable Catalytic Enantioselective Cyclopropanations of Diazo Carbonates.

A new kind of ()-10,10'-bibenzoquinoline (-BBQ) ligands with C-axis chirality and super-conjugated aromatic rings were rationally devised, and concisely and efficiently prepared in two steps by the classic Combes quinoline synthesis starting from the commercially available chiral ()-1,1'-binaphthalene-2,2'-diamine (BINAM). Specifically partnered with AgOTf, the new chiral ligands effectively and enantioselectively catalyzed the cyclopropanations of diazo oxindoles and α-arylidene diazo succinimides with aromatic alkenes in good to excellent enantioselectivities (up to 98%ee) with moderate to excellent yields (up to 89% yield). Our protocol has disclosed the first example of silver-catalyzed highly enantioselective cyclopropanation of diazo carbonates.

Epitranscriptomic Analysis of the Ventral Hippocampus in a Mouse Model of Post-Traumatic Stress Disorder Following Deep Brain Stimulation Treatment of the Basolateral Amygdala.

Background: Basolateral amygdala (BLA) deep brain stimulation (DBS) has been shown to alleviate the symptoms of post-traumatic stress disorder (PTSD), but the specific mechanisms remain incompletely understood. The hippocampus, a brain region closely connected to the amygdala, plays a key role in the pathological processes of PTSD. The N6-methyladenosine (mA) methylation of RNAs in the hippocampus is known to play a significant role in regulating the brain's response to stress and emotional disorders.

EHR Documentation Frequency Changes Across the COVID-19 Pandemic.

Documentation is crucial for patient care. However, the increased documentation burden raises questions about its clinical value. The COVID-19 pandemic provided an opportunity to explore nurses' documentation patterns due to increased patient care demands and the implementation of documentation relaxation policy.

Branching-Induced Intermolecular Repulsion Effects Drive Stable and Sustainable Flow Batteries on Condensed Nitroxyl Radicals.

Aqueous organic redox flow batteries (AORFBs) play a critical role in scalable energy storage applications where safety, cost, and lifetime matter most. However, harnessing the organics with redox chemistry is plagued by major molecular engineering principles in reversible processes and transformations. Herein, breaking from the conventional linear substituent structures, we report a chain-branched dual-ammonium nitroxyl radicals derivative as a stable and flowable catholyte for AORFBs.

Construction and evaluation of a nomogram prediction model for the risk of epistaxis following nasotracheal intubation: a single-center retrospective cohort study.

Background And Objective: Nasotracheal intubation (NTI) is a common procedure in oral and maxillofacial surgery; however, it is associated with a significant risk of post-procedural epistaxis. This study aims to identify risk factors for epistaxis following NTI and develop a nomogram-based prediction model to provide clinicians with a reliable risk assessment tool.

Methods: This single-center, retrospective study included 640 adult patients who underwent NTI at Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, between June 2020 and December 2021.

Millettia speciosa Champ. Leguminosae ameliorates testicular damage in mice by repairing of blood-testis barrier dysfunction and testosterone synthesis.

Ethnopharmacological Relevance: Millettia speciosa Champ. Leguminosae (MSC), a medicinal-edible plant in southern China, according to the records in Lingnan caiyaolu, MSC is known for its functions of "tonifying deficiency and consolidating essence." It is also empirically used to enhance male reproductive health.

Unveiling the molecular basis of selective fluorination of SAM-dependent fluorinases.

-Adenosylmethionine (SAM)-dependent fluorinases have emerged as environmentally friendly enzymatic alternatives for organofluorine chemical synthesis. However, their use remains limited by their rarity; only 16 fluorinases have been found in nature so far. Here we report two new fluorinases, FLA from and a modified FLA from .

Semi-automated pipeline to accelerate multi-site flowsheet alignment and concept mapping in electronic health records.

Objectives: Health-care institutions customize electronic health record (EHR) configurations to reflect their unique workflows and patient care priorities. Ensuring EHR alignment across sites facilitates seamless information exchange. We developed a pipeline for EHR flowsheet alignment between health-care organizations.