Evaluation of the lipolytic and antioxidant activities of different strains of coagulase-negative staphylococci in fermented sausages.

Six strains of coagulase-negative staphylococci, isolated from fermented meat products and identified as Staphylococcus hominis 9, Staphylococcus saprophyticus 18, Staphylococcus saprophyticus 27, Staphylococcus xylosus 37, Staphylococcus saprophyticus 120, and Staphylococcus cohnii 129, were evaluated for their lipid hydrolase and antioxidant enzyme activities. The effects of the selected strains on lipid hydrolysis and oxidation in fermented sausages were subsequently investigated. In meat simulation medium, Staphylococcus cohnii 129 exhibited the highest lipase and superoxide dismutase activities among all tested strains.

Dietary Interventions with Polysaccharides and/or Composite Polysaccharides Remodel Liver Lipid Profiles and Ameliorate Gut Metabolic Disturbances in High-Fat Diet-Induced Obese Mice.

The global obesity epidemic and associated metabolic disorders present urgent public health challenges. This study employed a multi-omics approach (lipidomics, metabolomics, and gut microbiome analysis) to investigate how polysaccharides (BSPs) and composite polysaccharides modulate liver lipid metabolism and gut microbiota in high-fat diet (HFD)-induced obese mice. HFD elevated hepatic phosphatidylcholines, cholesteryl esters (CEs), and acylcarnitines (CARs), alongside increased cecal choline and trimethylamine.

Emerging roles of KLF4 regulation of autophagy in different physiological and pathological processes.

Krüppel-like factor 4 (KLF4) is a zinc finger transcription factor that exhibits both transcriptional activation and inhibition effects. It participates in the occurrence and development of various diseases by regulating processes such as cell cycle arrest, differentiation, and the maintenance of stem cell pluripotency. Autophagy, as a conserved lysosome dependent degradation pathway in eukaryotes, maintains cellular homeostasis by clearing abnormal proteins and damaged organelles.

Characteristics of four natural poliovirus type 1 variants with six-nucleotide deletion (2,783-2,788 nt) in the VP1 region.

Since the establishment of the Chinese acute flaccid paralysis (AFP) case surveillance system in 1999, around 7,200 strains of poliovirus (PV) type 1 have been identified and isolated. Among these, the VP1 region of 5,649 strains has been sequenced. Based on the existing VP1 region sequence library, four strains of type 1 PV with six-nucleotide deletion in the VP1 region, identified from AFP cases, healthy children, and environmental sewage samples, were identified, and their biological characteristics were investigated.

The dual-edged sword: AlkB homolog 5-mediated autophagy regulation in cancers - molecular mechanisms and therapeutic implications: A review.

N-Methyladenosine (mA), the most prevalent internal modification in eukaryotic messenger RNA (mRNA), plays crucial, context-dependent (e.g., tumor type, stage, or microenvironmental conditions like hypoxia) roles in cancers.

Common bile duct dilatation on MRI in autosomal dominant polycystic kidney disease.

Purpose: Polycystic liver disease is the most prevalent extrarenal manifestation of autosomal dominant polycystic kidney disease (ADPKD). Non-obstructive asymptomatic common bile duct (CBD) dilatation has been observed anecdotally on CT scans, but CT is not optimal for biliary visualization. Here, we measured CBD diameter on T2-weighted MRI in ADPKD subjects to determine if CBD dilatation is associated with ADPKD.

Contrast-enhanced CT-Derived extracellular volume fraction predicts survival in postoperative pancreatic cancer patients.

Objective: To evaluate the prognostic value of extracellular volume (ECV) fraction derived from contrast-enhanced computed tomography (CECT) in predicting survival outcomes of resectable pancreatic ductal adenocarcinoma (PDAC).

Methods: The study cohort included a total of 166 patients with resectable PDAC who underwent CECT before operative excision between Jan 2018 and Mar 2021. Tumor CT-derived ECV fractions were calculated from unenhanced and equilibrium-phase CT images.

Effects of Pregnancy on Liver and Kidney Cyst Growth Rates in Autosomal Dominant Polycystic Kidney Disease: A Pilot Study.

: Polycystic liver disease (PLD) is the most common extrarenal manifestation of autosomal dominant polycystic kidney disease (ADPKD). PLD is more prevalent in women, and women have larger liver cysts, possibly due to estrogen-related mechanisms. Maternal estrogen levels normally increase during pregnancy.

[Clinical and genetic characteristics of osteopetrosis in children].

Objectives: To study the clinical and genetic characteristics of osteopetrosis (OPT) in children.

Methods: A retrospective analysis was performed on the clinical data of 14 children with OPT. Whole-exome sequencing was used to detect pathogenic genes, and clinical phenotypes and genotypic features were summarized.

Effects of Glutathione-Enriched Inactive Dry Yeast on the Flavor Profile of Kiwi Wine.

This study aimed to explore the influence of glutathione-enriched inactive dry yeast (g-IDY) addition on the changes in aroma and taste compounds in kiwi wine (KW), produced from green-, red-, and yellow-flesh kiwifruits. In total, 42 aroma compounds and 67 taste compounds were characterized using GC-IMS and H-NMR, respectively. Among them, six aroma compounds and thirty-one taste compounds were determined as key compounds based on a -test, variable importance in projection scores, and relative odor activity value.

Sensory and Omics approaches reveal the impact of heat treatment before fermentation on kiwi wine.

The study explores the effect of pre-fermentation heat treatment (PFHT) on the flavor and metabolomic profiles of kiwi wine (KW) derived from three kiwifruit cultivars. Six KW groups were involved, namely with/without PFHT for green (GWH/GW), yellow (YWH/YW), and red (RWH/RW) kiwifruit. E-tongue analysis effectively distinguished the taste profiles across these KW groups, identifying significant variations.

Physicochemical Properties, Antioxidant Activities, and Aromatic Profile of Yogurt Co-Fermented by G232 with Traditional Starters.

To improve the quality and functional properties of yogurts, a multi-starters co-fermentation system was used during yogurt preparation. In this work, G232 (added at 0%, 3%, 5%, and 7%) was involved as a co-fermenter with a traditional starter ( subsp. G119 and Q019).

Identification of a novel mutation in the gene in pediatric osteopetrosis: case report.

Osteopetrosis, also known as osteosclerosis and marble-bone disease, is a rare genetic metabolic bone disorder caused by the dysplasia or dysfunction of osteoclasts, usually caused by variants of chloride voltage-gated channel 7 ( gene. We retrospectively analyzed the clinical data of two children with osteopetrosis and their families. Whole-exome sequencing (WES) was used for genetic analysis, and Sanger sequencing confirmed possible pathogenic variants.

A semi-supervised convolutional neural network for diagnosis of pancreatic ductal adenocarcinoma based on EUS-FNA cytological images.

Background: The cytological diagnostic process of EUS-FNA smears is time-consuming and manpower-intensive, and the conclusion could be subjective and controversial. Moreover, the relative lack of cytopathologists has limited the widespread implementation of Rapid on-site evaluation (ROSE) presently. Therefore, this study aimed to establish an AI system for the detection of pancreatic ductal adenocarcinoma (PDAC) based on EUS-FNA cytological images.

The Role of Baseline Total Kidney Volume Growth Rate in Predicting Tolvaptan Efficacy for ADPKD Patients: A Feasibility Study.

: Although tolvaptan efficacy in ADPKD has been demonstrated in randomized clinical trials, there is no definitive method for assessing its efficacy in the individual patient in the clinical setting. In this exploratory feasibility study, we report a method to quantify the change in total kidney volume (TKV) growth rate to retrospectively evaluate tolvaptan efficacy for individual patients. Treatment-related changes in estimated glomerular filtration rate (eGFR) are also assessed.

166, Derived from Yak Yogurt from Tibetan Areas of Sichuan, Improves High-Fat-Diet-Induced Hyperlipidemia by Modulating Gut Microbiota and Liver- and Gut-Related Pathways.

The consumption of an unbalanced diet, such as a high-fat diet, is strongly associated with hyperlipidemia and significantly contributes to the development of cardiovascular and cerebrovascular diseases, which are the leading causes of death worldwide. Globally, about 17.9 million people die of cardiovascular disease each year (WHO 2023).

[Molecular Pathogenic Mechanism Study of Two Cases of Inherited Dysfibrinogenemia].

Objective: To analyze two families with inherited dysfibrinogenemia, and explore the molecular pathogenic mechanisms.

Methods: The coagulation indexes of the probands and their family members were detected. The , and exons and their flanking sequences were amplified by PCR, and the mutation sites were identified by sequencing.

Model-Assisted Spleen Contouring for Assessing Splenomegaly in Myelofibrosis: A Fast and Reproducible Approach to Evaluate Progression and Treatment Response.

: Accurate and reproducible spleen volume measurements are essential for assessing treatment response and disease progression in myelofibrosis. This study evaluates techniques for measuring spleen volume on abdominal MRI. : In 20 patients with bone marrow biopsy-proven myelofibrosis, 5 observers independently measured spleen volume on 3 abdominal MRI pulse sequences, 3D-spoiled gradient echo T1, axial single-shot fast spin echo (SSFSE) T2, and coronal SSFSE T2, using ellipsoidal approximation, manual contouring, and 3D nnU-Net model-assisted contouring comparing coefficients of variation.

Insights into the Flavor Profile of Yak Jerky from Different Muscles Based on Electronic Nose, Electronic Tongue, Gas Chromatography-Mass Spectrometry and Gas Chromatography-Ion Mobility Spectrometry.

It is well known that different muscles of yak exhibit distinctive characteristics, such as muscle fibers and metabolomic profiles. We hypothesized that different muscles could alter the flavor profile of yak jerky. Therefore, the objective of this study was to investigate the differences in flavor profiles of yak jerky produced by (LT), (TB) and (BF) through electronic nose (E-nose), electronic tongue (E-tongue), gas chromatography-mass spectrometry (GC-MS) and gas chromatography-ion mobility spectrometry (GC-IMS).

Real-world effectiveness of adjuvant octreotide therapy in patients with pancreatic neuroendocrine tumors at high recurrence risk: A multicenter retrospective cohort study.

Adjuvant therapy for pancreatic neuroendocrine tumors (PanNETs) after radical resection lacks evidence-based data and remains controversial. This study aimed to validate whether long-acting octreotide is a potential candidate for adjuvant therapy in patients with G2 PanNETs at high recurrence risk by clustering real-world data. A retrospective review of patients with nonmetastatic grade 2 PanNETs who underwent radical resection at six research centers between 2008 and 2020 was conducted.

Characterization of flavor profile of Steamed beef with rice flour using gas chromatography-ion mobility spectrometry combined with intelligent sensory (Electronic nose and tongue).

The intelligent senses (Electronic nose and tongue), were combined with headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and free amino acid were used in combination to determine the aroma and taste components during the processing of Chinese traditional dish Steamed beef with rice flour (SBD). The findings revealed that E-nose and E-tongue, could clearly distinguish and identify the aroma and taste of SBD. A total of 66 volatile substances and 19 free amino acids were identified by HS-GC-IMS and amino acid analyzer, respectively.

[Clinical phenotypes and genotypes of congenital fibrinogen disorder: an analysis of 16 children].

Objectives: To investigate the clinical phenotypes and genotypes of children with congenital fibrinogen disorder (CFD).

Methods: A retrospective analysis was conducted on the clinical data of 16 children with CFD. Polymerase chain reaction was used to amplify all exons and flanking sequences of the , , and genes, and sequencing was performed to analyze mutation characteristics.

Automatically Detecting Pancreatic Cysts in Autosomal Dominant Polycystic Kidney Disease on MRI Using Deep Learning.

Background: Pancreatic cysts in autosomal dominant polycystic kidney disease (ADPKD) correlate with PKD2 mutations, which have a different phenotype than PKD1 mutations. However, pancreatic cysts are commonly overlooked by radiologists. Here, we automate the detection of pancreatic cysts on abdominal MRI in ADPKD.

Prevalence of Spinal Meningeal Diverticula in Autosomal Dominant Polycystic Kidney Disease.

Background And Purpose: Patients with autosomal dominant polycystic kidney disease (ADPKD) develop cysts in the kidneys, liver, spleen, pancreas, prostate, and arachnoid spaces. In addition, spinal meningeal diverticula have been reported. To determine whether spinal meningeal diverticula are associated with ADPKD, we compared their prevalence in subjects with ADPKD with a control cohort without ADPKD.