OsMATE7-mediated flavonol accumulation regulates pollen tube growth in rice.

Flavonols have been implicated in male sterility and pollen tube growth for over three decades; however, the molecular mechanisms mediating their accumulation in pollen grains remain poorly understood. In this study, a multidrug and toxic compound extrusion (MATE) transporter, OsMATE7, was identified as a key regulator of flavonol accumulation in mature pollen grains, thereby promoting pollen tube growth in rice (Oryza sativa). Mutation of OsMATE7 resulted in a significant reduction in seed setting rates.

The relationship between different missed care activities and patient outcomes: A systematic review.

Background: Missed care is a key indicator of nursing quality and has been linked to negative patient outcomes. However, the rates of missed care vary across different nursing activities. Treating missed care as a composite indicator may lack specificity in relation to patient outcomes.

Case Report: Compound heterozygous variants in cause complex syndactyly with oligodactyly, renal artery variation, and facial scar.

Background: The gene, a member of the basic helix-loop-helix (bHLH) family of transcription factors, plays a critical role in limb development. Mutations in have been associated with various limb malformations, including syndactyly and split-hand/foot malformation. This study aimed to identify and characterize novel variants in a fetus with complex limb and renal abnormalities, providing further insights into the genetic basis of developmental disorders.

Corticospinal Excitability in Bilateral M1 Hand Areas: Association with Neuropathic Pain After Spinal Cord Injury.

Purpose: Neuropathic pain (NP) is a major and debilitating complication of spinal cord injury (SCI), frequently occurring bilaterally below the injury level. Repetitive transcranial magnetic stimulation (rTMS) targeting the primary motor cortex (M1) is suggested as a treatment for NP following SCI (SCI-NP). However, the specific changes in corticospinal excitability (CSE) within the bilateral M1 remain unclear, hindering the development of optimized rTMS parameters for SCI-NP.

Performance of heavy metals in the intertidal zone surface sediments of the Yellow River Estuary under water-sediment regulation scheme.

This study investigates the distribution, sources, and ecological risks of heavy metals (Cr, Cu, Zn, Cd, Pb, and Hg) and metalloid (As) in surface sediments of the Yellow River Estuary intertidal zone during the 2023 water-sediment regulation scheme (WSRS) period (summer) and the non-WSRS period (autumn). The results revealed that metal accumulation in both periods was primarily controlled by the co-adsorption of organic matter and fine particles. Hydrodynamic enhancement during the WSRS period reduced metal accumulation through sediment flushing and dilution, whereas weakened flows in the non-WSRS period promoted metal buildup.

Facile fabrication of functional xerogel membranes from sodium alginate-chitosan microfibers with low crystallinity through one-step solvent-exchange.

Biomass xerogels with porous structures fabricated in ambient conditions possess great advantages including scalability, biocompatibility, and biodegradability, thus have shown demonstrated potential in various fields. However, disadvantages such as poor moisture resistance, poor mechanical properties, and low production efficiency challenge their practical applications. In this study, microfibers self-assembled from sodium alginate and chitosan are adopted to facilely fabricate biomass porous xerogel membranes with a fast one-step solvent-exchange, which is achieved due to the low crystallinity and small aspect ratio of the microfibers.

Ultrasound-Based Z Score and Pulmonary Valve/Aortic Valve Diameter Ratio Improving the Predictive Value of Fetal Aortic Stenosis.

Objective: This study aims to investigate the predictive value of the ultrasound-based Z score for the aortic valve (AV) diameter, pulmonary valve (PV) Z score, and the PV/AV ratio in diagnosing aortic stenosis (AS) in fetuses.

Methods: A prospective study of 24 singleton fetuses with suspected AS who underwent two or more fetal echocardiograms was enrolled. Based on the pregnancy outcomes, the prenatal cases were divided into two groups: the AS group and the false-positive group.

Fabrication of sodium alginate nanofibrous membranes with bead-on-string structure for effective air filtration.

Airborne dust particles carrying pathogens threaten public health, driving demand for eco-friendly air filters. While sodium alginate (SA) offers sustainability advantages over petroleum-based nanofibers, its conventional smooth, densely packed membranes suffer from limited performance. Inspired by biological micro/nanostructures, we engineered bead-on-string SA nanofibers via green electrospinning.

Impact of HMGA1 on tumorigenesis, prognosis and immune microenvironment in HNSCC: a multi-omics study.

HMGA1 plays an important role in a variety of biological processes. However, it is still unclear what role HMGA1 plays in HNSCC. By integrating multi-omics data from public and private cohorts, we conducted a comprehensive analysis.

Analysis of clinical characteristics, diagnosis, treatment, and postoperative outcomes in deep infiltrating endometriosis: a retrospective study.

Objectives: To investigate the clinical characteristics of deep infiltrating endometriosis (DIE), summarize the experiences in its diagnosis and management, and identify potential peripheral blood indicators associated with the severity of pelvic adhesions.

Methods: Clinical data were collected from 185 patients with pathologically confirmed DIE admitted to Wuxi Maternal and Child Health Hospital between January 2015 and December 2024. A retrospective analysis was conducted on the general information, perioperative conditions, postoperative pregnancy outcomes, and recurrence status in patients with DIE.

Freeze-thaw durability and environmental risk assessment of silty soil improved using EICP combined with magnesite tailings powder.

Enzyme-induced carbonate precipitation (EICP) technology holds significant potential for applications in geotechnical engineering. While prior research has primarily focused on the mechanical enhancement of EICP-treated soils, limited attention has been paid to their long-term stability and durability. This study addresses the deterioration of foundation soils in the seasonally frozen region of the lower reaches of the Yellow River, where repeated freeze-thaw (FT) cycles pose a major challenge.

High-frequency repetitive transcranial magnetic stimulation protects against 6-OHDA-induced Parkinson's disease symptoms by modulating the proNGF-p75NTR-sortilin pathway.

Background: High-frequency repetitive transcranial magnetic stimulation (HF rTMS) is a promising non-invasive treatment for Parkinson's disease (PD) in clinical settings. However, the precise mechanisms are incompletely understood. The proNGF-p75NTR-sortilin signaling pathway is closely associated with nigrostriatal dopaminergic (DA) neuron degeneration, a hallmark feature of PD.

A learning behavior classification model based on classroom meta-action sequences.

The individual adaptive behavioral interpretation of students' learning behaviors is a vital link for instructional process interventions. Accurately recognizing learning behaviors and conducting a complete judgment of classroom meta-action sequences are essential for the individual adaptive behavioral interpretation of students' learning behaviors. This paper proposes a learning behavior classification model based on classroom meta-action sequences (ConvTran-Fibo-CA-Enhanced).

Cingulate retinoic acid signaling regulates neuropathic pain and comorbid anxiodepression via extracellular matrix homeostasis.

Neuropathic pain is often comorbid with affective disorders. Synaptic plasticity in anterior cingulate cortex (ACC) is assumed to be a crucial interface for pain perception and emotion. Laminin 1 (LAMB1), a key element of extracellular matrix (ECM) in ACC was recently revealed to convey extracellular alterations to intracellular synaptic plasticity and underlie neuropathic pain and aversive emotion.

The distribution patterns and clinical significance of serum immunoglobulin E in children with chalazion in Hubei, China.

Background: Chalazion is common in children which can lead to cosmetically disfiguring, ptosis, astigmatism, and affect the quality of life. The aim of this study was to investigate the distribution patterns of serum total immunoglobulin E (T-IgE) and specific immunoglobulin E (sIgE) levels in children with chalazia, further to explore the clinical significance of immunoglobulin E (IgE) in the development of chalazion.

Methods: This retrospective cross-sectional study enrolled children with chalazion or allergic conjunctivitis (AC) in Hubei, China, from June 2020 to December 2021.

Development and Application of an ACQUIRE Method for Direct Cloning of Superlarge Biosynthetic Gene Cluster.

Here, we developed a direct cloning method that aims to capture superlarge biosynthetic gene clusters (BGCs) (e.g., >150 kb), namely, ACQUIRE (dvanced as12a nuclease assisted precise and ck digestion of in-gel prepared genomic DNA in combination with the transformation-associated combination in yeast).

Transcriptome-wide gene-age interaction study and multi-omics comprehensive analysis reveals heterogeneous regulation of NLRC4 during aging on HNSCC prognosis and immune microenvironment.

Substantial age-related heterogeneity exists in the treatment of head and neck squamous cell carcinoma (HNSCC) patients, effective predictive guidance is needed to improve survival of elderly HNSCC patients, but it is unclear whether the effect of gene expression on HNSCC overall survival varies with age. By integrating transcriptome data from multiple centers, we identified one gene, NLRC4, whose effect was significantly modified by age on a transcriptome-wide scale (HR = 1.025, P = 8.

Targeting iron overload and macrophage polarization to treat diabetic periodontitis: Mechanisms and therapeutic strategies.

Diabetic periodontitis (T2DM-CP, type 2 diabetes mellitus-associated chronic periodontitis) is exacerbated by iron overload and macrophage imbalance, but therapeutic strategies remain limited. We analyzed macrophage polarization and iron metabolism markers in T2DM-CP patients and high glucose-treated macrophages, then evaluated deferoxamine (DFO) and chitosan hydrogel containing black phosphorus (BP) nanosheets and Dl-3-n-butylphthalide (NBP) (CH-BPNs-NBP) as targeted therapies. Clinical T2DM-CP samples and high glucose-stimulated macrophages showed increased M1 markers (iNOS) with decreased M2 markers (ARG-1), along with iron dysregulation (elevated TfR1, reduced FPN1 and GPX4).

OsPPR8, a pentatricopeptide repeat protein, regulates splicing of mitochondrial nad2 intron 3 to affect grain quality and high-temperature tolerance in rice.

Pentatricopeptide repeat (PPR) proteins, a large family of plant proteins, play critical roles in regulating various biological functions. However, their contributions to rice grain quality and high-temperature (HT) tolerance remain poorly understood. In this study, we identified OsPPR8, a canonical P-type PPR protein characterized by eight PPR motifs.

Structural variation in the promoter regulates the hull color of rice.

Unlabelled: Rice hull color (HC) is crucial for improving the mechanization efficiency of hybrid rice seed production. However, the genetic resources for hull color currently available for practical production are limited, highlighting an urgent need to explore natural variations that can be utilized in breeding. In this study, we conducted a genome-wide association study (GWAS) on the hull color of 301 rice germplasm resources and identified a significant quantitative trait locus (QTL) on chromosome 3.

Large Grain 2, an NHL Domain-Containing Protein, Interacts with FUWA and Regulates Plant Architecture and Grain Size Through the Brassinosteroid Signaling Pathway in Rice.

Plant architecture and grain size are critical traits for rice breeding. Brassinosteroid (BR), a class of plant hormones, regulates these traits by modulating cell elongation, division, and differentiation. Therefore, exploring BR-related genes to leverage their pleiotropic effects is crucial for crop improvement.

Locus Coeruleus Noradrenergic-Spinal Projections Contribute to Electroacupuncture-Mediated Antinociception in Postoperative Pain in Mice.

Postoperative pain remains a significant challenge in healthcare. Electroacupuncture (EA) has gained polarity in helping manage surgical pain and showed beneficial effects on enhancing postoperative analgesia, decreasing opioid requirement. Despite this, the precise mechanisms underlying these actions are poorly understood.

p16INK4a downregulation alleviates temporomandibular joint osteoarthritis combined with type 2 diabetes by driving M2 polarization.

Temporomandibular joint osteoarthritis (TMJOA), sometimes combined with type 2 diabetes (T2DM-TMJOA), has a restricting effect on quality of life and currently has few efficacious treatment options. As such, this project sought to determine the role of p16INK4a (p16) in T2DM-TMJOA development. In vivo, p16 knockout (P16KO) mice experienced less condylar bone loss and altered macrophage polarities from the inflammatory M1 to anti-inflammatory M2.

Poly(methyl methacrylate) Nanosphere-Based Photocrosslinked Hydrogels with Ultralong Phosphorescence Lifetimes for High-Precision 3D Printing.

Hydrogel-based afterglow materials offer significant potential for broadening the application field of organic room-temperature phosphorescence (RTP) materials owing to their tissue-mimetic flexibility and superior biocompatibility. However, achieving a colorful and efficient RTP in a water-rich hydrogel environment remains challenging. Here, we present a general strategy to fabricate colorful and efficient RTP hydrogels by incorporating compact and hydrophobic nanospheres loaded with chromophores, synthesized via emulsion polymerization, into photocrosslinked hydrogels with oxygen barrier properties.