Automated exo-glycosidase-based glycan sequencing on a digital microfluidic system with integrated electrochemical detection of monosaccharides.

Glycans are one of the most important biomacromolecules and are essential to nearly all known life forms. However, their structural complexity makes structural analysis more challenging than nucleic acids or proteins, significantly limiting the opportunities for investigating their structure-function relationship. Here, we proposed a novel glycan sequencing strategy in which a suite of specific non-reducing-end exoglycosidases was integrated with electrochemical detection of monosaccharides in a digital microfluidic (DMF) chip.

Empowering health through tradition: A grounded theory study on the impact of traditional Chinese physical activities on the well-being of full-time mothers.

This study explores the impact of Traditional Chinese Physical Activities (TCPAs), such as Tai Chi and group dancing, on the well-being of non-working full-time mothers. Using a grounded theory approach, semi-structured in-depth interviews were conducted with 40 full-time mothers actively engaged in TCPAs. Data were analyzed through open, axial, and selective coding.

Universal 3D-Printing of Suspended Metal Oxide Nanowire Arrays on MEMS for AI-Optimized Combinatorial Gas Fingerprinting.

Additive manufacturing technology has the potential to provide great versatility in the design and fabrication of sensing devices. This prerequisite necessitates further technological improvement in precision and material diversity. Here, a universal meniscus-guided 3D printing method is reported that can fabricate freestanding metal oxide semiconducting nanowires with programmed compositions and compatible substrate options at the single-entity level.

Attention-based deep learning network for predicting World Health Organization meningioma grade and Ki-67 expression based on magnetic resonance imaging.

Objectives: Preoperative assessment of World Health Organization (WHO) meningioma grading and Ki-67 expression is crucial for treatment strategies. We aimed to develop a fully automated attention-based deep learning network to predict WHO meningioma grading and Ki-67 expression.

Materials And Methods: This retrospective study included 952 meningioma patients, divided into training (n = 542), internal validation (n = 96), and external test sets (n = 314).

Research on Ground Point Cloud Segmentation Algorithm Based on Local Density Plane Fitting in Road Scene.

In road scenes, the collected 3D point cloud data is usually accompanied by a large amount of interference mainly composed of ground point clouds and the property of uneven density distribution, which will bring difficulties to subsequent recognition and prediction. To address these problems, this paper proposes a ground point cloud segmentation algorithm based on local density plane fitting. Firstly, for the uneven density distribution of 3D point clouds, density segmentation is used to obtain several regions with balanced density.

3D imaging of moving cells within droplets with a staggered bifocal microlens array.

Three-dimensional (3D) observation of moving cells offers critical insights into fundamental biological processes. However, the limited temporal resolution of traditional scanning-based 3D imaging techniques constrains their use in live-cell imaging. Light-field microscopy (LFM) has emerged as a scanning-free alternative, enabling rapid 3D volumetric data acquisition.

mRNA-LNP Vaccines Targeting SmpA-PLD and OmpK-Omp22 Induce Protective Immunity Against .

Background: () has emerged as a critical human pathogen, causing high mortality rates among hospitalized patients and frequently triggering nosocomial outbreaks. The increasing prevalence of multidrug-resistant (MDR) poses a pressing threat to public health. To date, no commercially available vaccine against has been developed for clinical use.

Interfacial electron transfer triggered reversible hydrogen spillover effect boosts high-efficient hydrogen evolution.

The mechanistic role of interfacial electron transfer in enabling reversible hydrogen spillover for enhanced hydrogen evolution reaction (HER) kinetics remains fundamentally unexplored. Herein, we report a lattice-matched PtCoNiCuZn/ WCN heterostructure electrode, where interfacial electron transfer triggers reversible hydrogen spillover, thereby achieving exceptional HER performance. Advanced characterization reveals coherent and semicoherent interfaces that induce charge redistribution, creating electron-deficient Pt sites with optimally downshifted d-band centers.

Transarterial chemoembolization combined with apatinib in the treatment of advanced hepatocellular carcinoma: a prospective, multi-center, real-world study (Ahend-HAP02).

Purpose: To analyze the efficacy and safety of transarterial chemoembolization (TACE) with apatinib (TACE-A) for the treatment of advanced hepatocellular carcinoma (HCC).

Methods: Data from advanced HCC patients treated with TACE-A between January 2019 and June 2022 were evaluated. The patients from 8 medical centers were included.

Eosinophil counts predict 3-year risk of major adverse cardiovascular and cerebrovascular events in carotid artery stenosis patients.

Eosinophils are a part of the human immune system and have traditionally been thought to be associated with allergic reactions. Recent research has revealed their potentially important role in cardiovascular disease. We retrospectively collected data on 1155 patients who were diagnosed with carotid artery stenosis at our center from January 2016 to June 2021.

Enhancement mechanism of electron-phonon coupling in B ( = K and Rb) compounds with Kagome lattice.

Kagome superconductors are a recent hot topic in superconductivity, while most studies focus on ternary systems. This study investigated the Kagome lattice and other structure properties of binary B ( = K and Rb) compounds in the pressure range of 0-30 GPa using crystal structure prediction and first-principles calculations. The crystal properties and bonding characteristics of the , 4/, 6/, and 6/ phases were analyzed.

Metabolomic profiling uncovers diagnostic biomarkers and dysregulated pathways in Parkinson's disease.

Background: Parkinson's disease (PD) is the second most common neurodegenerative disorder, and it has an unclear pathogenesis and lacks validated, specific biomarker-based diagnostic approaches, particularly in PD patients with rapid eye movement (REM) sleep behavior disorder (PD-RBD).

Methods: Using untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics, serum profiles of 41 drug-naïve PD patients [including 19 PD-RBD and 22 PD without RBD (PD-nRBD) patients] and 20 healthy controls (HCs) were analyzed.

Results: Comparative analyses revealed 144 dysregulated metabolites in PD patients versus HCs, with 7 metabolites-sodium deoxycholate, S-adenosylmethionine, L-tyrosine, 3-methyl-L-tyrosine, 4,5-dihydroorotic acid, (6Z)-octadecenoic acid, and allantoin-demonstrating high classification accuracy [area under the curve (AUC) > 0.

RETINA: Reconstruction-based pre-trained enhanced TransUNet for electron microscopy segmentation on the CEM500K dataset.

Electron microscopy (EM) has revolutionized our understanding of cellular structures at the nanoscale. Accurate image segmentation is required for analyzing EM images. While manual segmentation is reliable, it is labor-intensive, incentivizing the development of automated segmentation methods.

Study on remediation capacity of typical forage grasses for Cu, Pb, and Cd contamination in coal gangue-accumulated soils.

Based on the ecological restoration needs of coal gangue accumulation areas, this study focuses on the potential application of in situ remediation techniques using forage plants. Taking the heavy metal pollution characteristics of the shallow soil (0-20 cm) in a typical coal gangue accumulation area in the Fengfeng mining district of Hebei as a prototype, pot experiments were conducted to investigate the heavy metal content in soil and plants under different heavy metal concentration gradients of Cu (14, 64, 100 mg/kg), Cd (1, 4, 8 mg/kg), and Pb (15, 38, 170 mg/kg) for both single and composite pollution types, revealing the heavy metal accumulation coefficients and translocation capabilities of alfalfa Medicago sativa L.(alfalfa) and Lolium perenne L.

The impact of nuchal ligament ossification resection on cervical stability after modified laminoplasty: a long-term follow-up study.

Study Design: A retrospective study.

Objectives: This study aims to investigate the relationship between the resection of ossification of the nuchal ligament (ONL), its morphological features, and cervical stability following modified laminoplasty.

Methods: We retrospectively reviewed the data of patients diagnosed as degenerative cervical myelopathy (DCM) who underwent modified laminoplasty with muscle-ligament complex (MLC) reconstruction in our hospital between July 2018 and October 2022.

Impact of frailty on short- and long-term outcomes following cervical laminoplasty for ossification of the posterior longitudinal ligament: a retrospective cohort study.

Purpose: To evaluate the impact of frailty, as measured by the 5-item modified frailty index (mFI-5), on short- and long-term postoperative outcomes in patients undergoing cervical posterior laminoplasty for ossification of the posterior longitudinal ligament (OPLL).

Study Design: A retrospective cohort study.

Methods: 198 patients who underwent cervical posterior laminoplasty were divided into three groups based on mFI-5 scores: Group A (0 point), Group B (1 point), and Group C (2 or more points).

Semi-permeable membrane stabilized microfluidic plasma chip for continuous, tunable synthesis of sub-10 nm nanoparticles.

Atmospheric-pressure microplasma, characterized by its gaseous electrode containing tunable electrons and reactive species, can initiate reactions at the plasma/liquid interface. Integrating microplasma into a microfluidic chip can confine reactions to the microscale, enhancing uniformity and controllability. However, maintaining a stable gas/liquid interface in microchannels is inherently challenging due to Rayleigh-Plateau instability and perturbing pressure gradients.

DNMT1-Induced Downregulation of CBX7 Inhibits ERK Phosphorylation and Promotes Pancreatic Ductal Adenocarcinoma Progression.

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive cancer types, characterized by an alarmingly low 5-year survival rate. DNA methylation has been implicated in the progression of various tumors, with DNA methyltransferase 1 (DNMT1) being the most extensively studied enzyme in this context. However, the expression patterns and underlying mechanisms of DNMT1 in PDAC remain poorly understood.

Optimal dosage and modality of exercise on glycemic control in people with prediabetes: a systematic review and network meta-analysis.

Aims: This study aims to assess the effects of different exercise types and their specific doses on glycemic control among individuals with prediabetes.

Methods: Multiple databases were subjected to a comprehensive search for randomized controlled trials (RCTs) published until 15 July 2024. The study protocol was prospectively registered with PROSPERO (CRD42024573186).

Silencing of lncRNA PRR34-AS1 Alleviates Alzheimer's Disease by Targeting miR-29c-3p to Regulate Microglia Inflammation.

Objective: This study aims to investigate the role of long non-coding RNA (lncRNA) PRR34 antisense RNA 1 (PRR34-AS1) and microRNA (miR)-29c-3p in Alzheimer's disease (AD) and to explore their mechanisms.

Methods: The study included 35 AD patients and 35 healthy controls. In vitro experiments were conducted using microglial cell lines HMC3 and BV2, which were treated with Aβ25-35, and gene knockout or overexpression experiments were performed to verify the function of the target genes.

PD-L1 levels, TP53 mutation profiles, and survival outcomes in pancreatic cancer differ by immune-nutritional status.

Background: Pancreatic ductal adenocarcinoma (PDAC) frequently exhibits an immunosuppressive microenvironment coupled with malnutrition status. These features are instrumental in clinical management strategies for PDAC.

Methods: Immune-nutrition status of patients was evaluated by integrating systemic immune-inflammatory index (SII) and prognostic nutritional index (PNI).

Design, Synthesis, and Biological Evaluation of Selective STING Synergists That Enhance cGAMP-STING Pathway Activation without Inherent Agonist Activity.

The cGAS-STING pathway is pivotal for innate immunity and antitumor responses. However, the challenge of selectively targeting the diseased tissue without harming the healthy tissue has impeded the development of STING agonists. In this article, we tackle this issue by developing novel STING synergists that target the STING C-terminal domain pocket.

Homogeneous polymer-ionic solvate electrolyte with weak dipole-dipole interaction enabling long cycling pouch lithium metal battery.

Solid polymer electrolytes (SPEs) are considered as promising electrolytes for high-voltage lithium metal batteries. Whereas, the strong dipole-dipole interaction in polymer electrolytes limits the enhancement of the ionic conductivity. Here, we propose the 1,1,2,2-Tetrafluoroethyl-2,2,3,3-Tetrafluoropropylether (TTE) diluent to significantly regulate the dipole-dipole interaction in polymer-ionic solvate electrolytes (TPISEs).

Emergence of Small Nucleic Acids as Drugs in Precision Medicine.

Small nucleic acid drugs including antisense oligonucleotides, small interfering RNAs, microRNAs, and aptamers, among others, have revolutionized the pharmaceutical industry in the 21st century, offering novel therapeutic strategies for a myriad of diseases through precise gene expression regulation or protein synthesis modulation. As of December 2024, 19 small nucleic acid drugs have been approved globally, with applications primarily in treating rare and hereditary conditions, metabolic diseases, and ophthalmological disorders, demonstrating great clinical potential. This review provides an overview of the classification, mechanism of action, technical challenges, and addressing strategies for small nucleic acid drugs, with a focus on the indications for market-available drugs, aiming to equip healthcare professionals with a thorough understanding and practical guidance on their utilization.