Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress.

Cadmium (Cd), a highly toxic non-essential heavy metal, disrupts cellular processes even at low concentrations. The current studies of Cd stress rely on whole-plant models with prolonged treatments, obscuring early response signals. Tobacco, a typical model plant, lacks protoplast-level investigations under Cd stress.

Impact of Gut and Reproductive Tract Microbiota on Estrogen Metabolism in Endometriosis.

Background: The incidence of endometriosis is rising, particularly among younger populations, yet current diagnostic and therapeutic approaches remain limited. This highlights the urgent need for novel diagnostic tools and effective treatments. Recent studies have shown that intestinal and reproductive tract bacterial dysbiosis is closely associated with the development of endometriosis and plays a key role in the regulation of estrogen metabolism.

A cascaded tapered seven-core fiber biosensor for rapid detection of the p53 protein in 293T cell lysate.

P53 protein is one of the main cancer biomarkers, and its detection is of great significance for early diagnosis and treatment of various cancers such as breast, rectal, and lung cancer. In this paper, a cascaded tapered seven-core fiber (CTSCF) biosensor is proposed for rapid detection of p53 protein. The experiment results show that the proposed CTSCF biosensor can effectively detect the p53 protein at concentrations of 0.

NtSHN3 directly inhibits the expression of NtF6'H1 and NtTOGT1 genes to prevent the accumulation of scopoletin in Nicotiana tabacum.

Scopoletin is a kind of coumarins, which play essential roles in plant development and human health. SHN1/WIN1 has been shown to promote polyphenols biosynthesis in tobacco, but the roles of its homologous protein SHN3 in regulating secondary metabolite accumulation are still unclear. In this study, we identified a new SHINE-type transcription factor NtSHN3 from N.

Efficient Sleep, Enhanced Attention: Exploring the Interplay With RBC-Inflammation Mechanisms in Hypoxic High-Altitude Areas.

Purpose: The complex interplay between sleep and attention, especially in the suppression of environmental information, is not well understood. This study investigates the bidirectional influence between sleep quality and executive control-an essential aspect of attention-and seeks to uncover the biological pathways involved in this relationship in hypoxic high-altitude areas.

Patients And Methods: We recruited 140 han Chinese juniors from Tibet University, all originally from lowland areas.

PD-L1 levels, TP53 mutation profiles, and survival outcomes in pancreatic cancer differ by immune-nutritional status.

Background: Pancreatic ductal adenocarcinoma (PDAC) frequently exhibits an immunosuppressive microenvironment coupled with malnutrition status. These features are instrumental in clinical management strategies for PDAC.

Methods: Immune-nutrition status of patients was evaluated by integrating systemic immune-inflammatory index (SII) and prognostic nutritional index (PNI).

The transhiatal tunnel valvuloplasty technique following laparoscopic proximal gastrectomy: the single-center experience in a retrospective cohort.

Background: The debate over the optimal reconstruction technique following proximal gastrectomy continues. Transhiatal tunnel valvuloplasty (ThTV) is a novel esophagogastrostomy method. This study aimed to evaluate the feasibility and safety of ThTV.

Survival and Metastatic Lymph Node Patterns in Gastric Carcinoma with Exocrine and Neuroendocrine Components.

Background: Gastric carcinoma with coexisting exocrine and neuroendocrine components (GC-EN) is a rare and aggressive subtype of gastric cancer that presents unique challenges in diagnosis, treatment, and prognosis. The effect of lymph node metastasis with different components on survival in patients with GC-EN is unknown.

Patients And Methods: This retrospective study included 98 patients diagnosed with GC-EN at the China National Cancer Center between January 2004 and December 2020.

Both direct and indirect suppression of MCL1 synergizes with BCLXL inhibition in preclinical models of gastric cancer.

Despite the progress of treatment in gastric cancer (GC), the overall outcomes remain poor in patients with advanced diseases, underscoring the urgency to develop more effective treatment strategies. BH3-mimetic drugs, which inhibit the pro-survival BCL2 family proteins, have demonstrated great therapeutic potential in cancer therapy. Although previous studies have implicated a role of targeting the cell survival pathway in GC, the contribution of different pro-survival BCL2 family proteins in promoting survival and mediating resistance to current standard therapies in GC remains unclear.

Association between adjuvant radiotherapy in adults with gastric cancer and risk of second primary malignancy: a retrospective cohort study using the Surveillance, Epidemiology and End Results database.

Objectives: This study aims to assess the association between adjuvant radiotherapy and the development of second primary malignancies (SPMs) and identify its determinants in patients who have undergone surgical treatment for gastric cancer.

Design: Retrospective cohort study using the Surveillance, Epidemiology and End Results (SEER) database.

Setting: Cohorts (18 registries, 2000-2018, from SEER) were screened for any malignancy that developed after sufficient latency from diagnosis of surgically treated non-metastatic gastric cancer.

Transcriptomic profiling and machine learning reveal novel RNA signatures for enhanced molecular characterization of Hashimoto's thyroiditis.

While ultrasonography effectively diagnoses Hashimoto's thyroiditis (HT), exploring its transcriptomic landscape could reveal valuable insights into disease mechanisms. This study aimed to identify HT-associated RNA signatures and investigate their potential for enhanced molecular characterization. Samples comprising 31 HT patients and 30 healthy controls underwent RNA sequencing of peripheral blood.

Genome-wide analysis of IDD genes identifies as a regulator of leaf angle.

The INDETERMINATE DOMAIN (IDD) gene family, encoding a class of C2H2 transcription factor, played diverse roles in land plants. The IDD family in tobacco () has not been characterized. In this study, 26 NtIDDs were identified in the tobacco genome.

Preparation and Performance Evaluation of CO Foam Gel Fracturing Fluid.

The utilization of CO foam gel fracturing fluid offers several significant advantages, including minimal reservoir damage, reduced water consumption during application, enhanced cleaning efficiency, and additional beneficial properties. However, several current CO foam gel fracturing fluid systems face challenges, such as complex preparation processes and insufficient viscosity, which limit their proppant transport capacity. To address these issues, this work develops a novel CO foam gel fracturing fluid system characterized by simple preparation and robust foam stability.

A sequential stimuli-responsive hydrogel promotes structural and functional recovery of severe spinal cord injury.

Utilizing drug-loaded hydrogels to restore nerve conductivity emerges as a promising strategy in the treatment of spinal cord injury (SCI). However, many of these hydrogels fail to deliver drugs on demand according to the dynamic SCI pathological features, resulting in poor functional recovery. Inspired by the post-SCI microenvironments, here we report a time-sequential and controllable drug delivery strategy using an injectable hydrogel responsive to reactive oxygen species (ROS) and matrix metalloproteinases (MMPs).

Probe on the pivotal role of green rust in improving the enzymatic activity and facilitating the formation of O in Fenton-like process for degradation of 4-chlorophenol.

Iron-based materials have demonstrated significant efficacy in catalyzing hydrogen peroxide (HO) for the removal of antibiotics from aquatic environments. Green rust (GR), a hybrid valence state iron-based catalyst, was synthesized. By exploiting the catalytic properties of glucose oxidase (GOx) to generate HO from glucose (Glu), a GR-GOx/Glu system for the removal of recalcitrant organic compound 4-chlorophenol (4-CP) was constructed.

Clinical and chest CT manifestations of Talaromycosis marneffei: a retrospective study comparing anti-IFN-γ autoantibodies-positive and HIV-positive cases.

Background: To explore the clinical and chest CT features of Talaromycosis marneffei (TSM), and to compare the differences between anti‑IFN‑γ autoantibodies‑positive and HIV-positive cases.

Methods: Clinical data and chest CT images of 54 HIV-negative patients with Talaromyces marneffei (TM) infection and positive anti-interferon-γ (anti-IFN-γ) autoantibody were retrospectively analyzed. Ninety-three HIV-positive patients with TM infection during the same period were included as controls.

Integrated Transcriptome Analysis Reveals Novel Molecular Signatures for Schizophrenia Characterization.

Schizophrenia (SCZ) is a complex psychiatric disorder presenting challenges for characterization. The current study aimed to identify and evaluate disease-responsive essential genes (DREGs) to enhance the molecular characterization of SCZ. RNA-sequencing data from PsychENCODE (536 SCZ patients, 832 controls) and peripheral blood transcriptome data from 144 recruited subjects (59 SCZ patients, 6 non-SCZ psychiatric patients, 79 controls) are analyzed.

Downstaging Effect Rather than the Full Intended Cycles of Perioperative Chemotherapy Determines the Value of Adjuvant Chemotherapy in Gastric Cancer.

Background: Perioperative chemotherapy is the standard treatment modality for locally advanced gastric cancer. However, the efficacy and indication of adjuvant chemotherapy in patients who have already received neoadjuvant chemotherapy remain unclear. This study aims to explore the association between adjuvant chemotherapy with patient prognosis in those who have received neoadjuvant chemotherapy plus D2 gastrectomy in a real-world setting, and whether this association is affected by the duration of neoadjuvant treatment.

Perioperative chemotherapy improves survival of patients with locally advanced diffuse gastric cancer.

Background: Whether patients with diffuse gastric cancer, which is insensitive to chemotherapy, can benefit from neoadjuvant or adjuvant chemotherapy has long been controversial.

Aim: To investigate whether perioperative chemotherapy can improve survival of patients with locally advanced diffuse gastric cancer.

Methods: A total of 2684 patients with locally advanced diffuse gastric cancer from 18 population-based cancer registries in the United States were analyzed.

Ultrasensitive detection of SCCA employing a graphene oxide integrated microfiber ring laser biosensor.

Squamous cell carcinoma antigen (SCCA) is one of the most commonly detected cancer biomarkers for a variety of cancers. In this paper, a microfiber ring laser biosensor with a graphene oxide linking layer for SCCA detection was proposed and experimentally demonstrated. SCCA antibody immobilized on graphene oxide surface binds specifically to SCCA, and induces refractive index variation over the surface of the microfiber biosensor, which leads to a wavelength shift of the microfiber ring laser biosensor.