Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress.

Cadmium (Cd), a highly toxic non-essential heavy metal, disrupts cellular processes even at low concentrations. The current studies of Cd stress rely on whole-plant models with prolonged treatments, obscuring early response signals. Tobacco, a typical model plant, lacks protoplast-level investigations under Cd stress.

Hierarchical flower-like CoO/AlO as headspace solid-phase microextraction fiber coating for enrichment and detection of polychlorinated biphenyls.

Polychlorinated biphenyls (PCBs) are known to pose serious risks to human health, including carcinogenicity, neurotoxicity and endocrine disruption. Due to these hazards to human health, development of sensitive and reliable methods for analysis of PCBs attracts considerable attention. Herein, a hierarchical flower-like CoO/AlO composite was employed as new coating material of headspace solid-phase microextraction (HS-SPME) for the first time.

Two functionalized metal-organic frameworks with multiple hydrogen-bond donors as SALDI-TOF-MS matrix for sensitive and rapid determination of pesticides.

Surface-assisted laser desorption/ionization-time of flight-mass spectrometry (SALDI-TOF-MS) generally possess difficulties for analysis of small-molecules. The development of novel matrices as alternatives to conventional matrices has shown to be an effective strategy for mitigating the interference in low molecular weight. In this study, two functionalized metal-organic frameworks with multiple hydrogen-bond donors (UIO-66(Zr)-MHD and MIL-100(Fe)-MHD) were synthesized and used as matrices in the laser desorption/ionization (LDI) process.

Whirly Transcription Factor NtWHY1 Positively Regulates the Biosynthesis of Cembranoid Diterpenoids by Directly Targeting NtCBTS in Tobacco.

Cembranoid diterpenoids, as crucial secondary metabolites in tobacco, play significant physiological roles and exhibit notable biological activities, while the transcriptional regulators governing their biosynthesis remain largely unexplored. A whirly transcription factor NtWHY1 is screened out by DNA pull down using the promoter of NtCBTS (cembratrien-ol synthase), a known key gene in the pathway of cembranoid diterpenoid biosynthesis. Further experiments revealed that NtWHY1 encodes a protein with dual localization in chloroplasts and the nucleus, and it is highly transcriptionally active in tobacco's glandular trichomes and leaves.

3D microporous metal-organic framework Ca-TCPB as a fiber coating for headspace solid-phase microextraction of polycyclic aromatic hydrocarbons.

Polycyclic aromatic hydrocarbons (PAHs) as a type of persistent organic pollutants in environment can pose serious threat to ecosystem and human health. Herein, a microporous metal-organic framework (MOF) material namely Ca-TCPB [HTCPB: 1,2,4,5-tetrakis(4-carboxyphenyl)-benzene] with three-dimensional (3D) structure was synthesized via simple solvothermal method. The Ca-TCPB served as fiber coating of solid-phase microextraction (SPME) to extract and enrich PAHs prior to gas chromatography analysis.

UiO-66 with missing cluster defects for high-efficient extraction and enrichment of benzoylurea insecticides.

The creation of defects in crystalline structures can tune metal-organic frameworks (MOFs) properties, such as improving their adsorptive and catalytic performance with producing more porosity and active sites. In this work, the bimetallic UiO-66 containing Zn and Zr was prepared. And then UiO-66 with missing cluster defects (UiO-66-1/3) were obtained by acid washing to remove the Zn nodes.

Enhancing coverage of annotated compounds in traditional Chinese medicine formulas: Integrating MS and Fast-DDA molecular network with AntDAS-Case study of Xiao Jian Zhong Tang.

The chemical characterisation of traditional Chinese medicine formulas (TCMFs) using mass spectrometry poses notable challenges owing to their complex and diverse chemical compositions. While acquisition modes such as data-dependent acquisition (DDA) and data-independent acquisition (DIA) offer new insights, DDA's tendency to overlook low-abundance ions and DIA's complicated data processing, particularly in matching MS and MS information, limit the effective annotation of valuable compounds in TCMFs. Herein, we present a new integrated strategy to enhance the coverage of annotated compounds in TCMFs, using Xiao Jian Zhong Tang (XJZ) as a case study.

NtERF283 positively regulates water deficit tolerance in tobacco (Nicotianatabacum L.) by enhancing antioxidant capacity.

Ethylene responsive factor (ERF) is a plant-specific transcription factor that plays a pivotal regulatory role in various stress responses. Although the genome of tobacco harbors 375 ER F genes, the functional roles of the majority of these genes remain unknown. Expression pattern analysis revealed that NtERF283 was induced by water deficit and salt stresses and mainly expressed in the roots and leaves.

Genome-wide analysis of long noncoding RNAs in response to salt stress in Nicotiana tabacum.

Background: Long noncoding RNAs (lncRNAs) have been shown to play important roles in the response of plants to various abiotic stresses, including drought, heat and salt stress. However, the identification and characterization of genome-wide salt-responsive lncRNAs in tobacco (Nicotiana tabacum L.) have been limited.

Identification and characterization of TMV-induced volatile signals in Nicotiana benthamiana: evidence for JA/ET defense pathway priming in congeneric neighbors via airborne (E)-2-octenal.

Plants release a mixture of volatile compounds when subjects to environmental stress, allowing them to transmit information to neighboring plants. Here, we find that Nicotiana benthamiana plants infected with tobacco mosaic virus (TMV) induces defense responses in neighboring congeners. Analytical screening of volatiles from N.

Metabolic engineering of a 1,8-cineole synthase enhances aphid repellence and increases trichome density in transgenic tobacco (Nicotiana tabacum L.).

Background: The green peach aphid (Myzus persicae Sulzer) is a harmful agricultural pest that causes severe crop damage by directly feeding or indirectly vectoring viruses. 1,8-cineole synthase (CINS) is a multiproduct enzyme that synthesizes monoterpenes, with 1,8-cineole dominating the volatile organic compound profile. However, the relationship between aphid preference and CINS remains elusive.

Temporal transcriptome analysis reveals several key pathways involve in cadmium stress response in L.

Tobacco has a strong cadmium (Cd) enrichment capacity, meaning that it can absorb large quantities from the environment, but too much Cd will cause damage to the plant. It is not yet clear how the plant can dynamically respond to Cd stress. Here, we performed a temporal transcriptome analysis of tobacco roots under Cd treatment from 0 to 48 h.

Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious Maxim Leaves during Reproductive Stages.

Leaves are essential vegetative organs of plants. Studying the variations in leaf nutrient content and microbial communities of male and female plants at reproductive stages helps us understand allocation and adaptation strategies. This study aimed to determine the nutrient characteristics and microbial differences in the leaves of male and female at reproductive stages.

CAR modulates plasma membrane nano-organization and immune signaling downstream of RALF1-FERONIA signaling pathway.

In Arabidopsis, the receptor-like kinase (RLK) FERONIA (FER) senses peptide ligands in the plasma membrane (PM), modulates plant growth and development, and integrates biotic and abiotic stress signaling for downstream adaptive responses. However, the molecular interplay of these diverse processes is largely unknown. Here, we show that FER, the receptor of Rapid Alkalinization Factor 1 (RALF1), physically interacts with C2 domain ABA-related (CAR) proteins to control the nano-organization of the PM.

Genome-wide identification and characterization of NPF family reveals NtNPF6.13 involving in salt stress in .

Proteins of the Nitrate Transporter 1/Peptide Transporter (NPF) family transport a diverse variety of substrates, such as nitrate, peptides, hormones and chloride. In this study, a systematic analysis of the tobacco () family was performed in the cultivated 'K326'. In total, 143 genes were identified and phylogenetically classified into eight subfamilies, NPF1 to NPF8, based on the classification of NPF families in other plant species.

Soil Quality Assessment in Tourism-Disturbed Subtropical Mountain Meadow Areas of Wugong Mountain, Central Southeast China.

Meadow soil is a vital ecosystem component and can be influenced by meadow vegetation. Evaluating soil quality in mountain meadows subjected to different levels of tourism disturbance is essential for scientific research, ecological restoration, and sustainable management. This study aimed to evaluate meadow soil quality at different tourism-disturbance levels and attempted to establish a minimum data set (MDS) with compatible indicators for soil quality assessment of subtropical mountain meadows.

The RALF1-FERONIA complex interacts with and activates TOR signaling in response to low nutrients.

Target of rapamycin (TOR) kinase is an evolutionarily conserved major regulator of nutrient metabolism and organismal growth in eukaryotes. In plants, nutrients are remobilized and reallocated between shoots and roots under low-nutrient conditions, and nitrogen and nitrogen-related nutrients (e.g.

A new platform for untargeted UHPLC-HRMS data analysis to address the time-shift problem.

Substantial deviations in retention times among samples pose a great challenge for the accurate screening and identifying of metabolites by ultrahigh-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). In this study, a coarse-to-refined time-shift correction methodology was proposed to efficiently address this problem. Metabolites producing multiple fragment ions were automatically selected as landmarks to generate pseudo-mass spectra for a coarse time-shift correction.

Proteomic and metabolomic analysis of Nicotiana benthamiana under dark stress.

Exposure to extended periods of darkness is a common source of abiotic stress that significantly affects plant growth and development. To understand how Nicotiana benthamiana responds to dark stress, the proteomes and metabolomes of leaves treated with darkness were studied. In total, 5763 proteins and 165 primary metabolites were identified following dark treatment.

Reconstruction of the full-length transcriptome of cigar tobacco without a reference genome and characterization of anion channel/transporter transcripts.

Background: Cigar wrapper leaves are the most important raw material of cigars. Studying the genomic information of cigar tobacco is conducive to improving cigar quality from the perspective of genetic breeding. However, no reference genome or full-length transcripts at the genome-wide scale have been reported for cigar tobacco.

Overexpression of OsRLCK241 confers enhanced salt and drought tolerance in transgenic rice (Oryza sativa L.).

Receptor-like cytoplasmic kinases (RLCKs) have been demonstrated to be involved in the regulation of growth, development, and pathogen responses in plants. However, the identity of RLCKs involved in abiotic tolerance remains elusive. In this study, we present data on OsRLCK241, a receptor-like cytoplasmic kinase that is induced by salt and drought stresses.

Characterization of the soybean R2R3-MYB transcription factor GmMYB81 and its functional roles under abiotic stresses.

R2R3-type MYBs are a key group of regulatory factors that control diverse developmental processes and stress tolerance in plants. Soybean is a major legume crop with the richness of seed protein and edible vegetable oil, and 244 R2R3-type MYBs have been identified in soybean. However, the knowledge regarding their functional roles has been greatly limited as yet.

Integrating transcriptome and metabolome reveals molecular networks involved in genetic and environmental variation in tobacco.

Tobacco (Nicotiana tabacum) is one of the most widely cultivated commercial non-food crops with significant social and economic impacts. Here we profiled transcriptome and metabolome from 54 tobacco samples (2-3 replicates; n = 151 in total) collected from three varieties (i.e.

FERONIA phosphorylates E3 ubiquitin ligase ATL6 to modulate the stability of 14-3-3 proteins in response to the carbon/nitrogen ratio.

The ratio between carbon (C) and nitrogen (N) utilization must be precisely coordinated to enable plant growth. Although numerous physiological studies have examined carbon/nitrogen (C/N) ratios, the mechanisms of sensing the C/N balance and C/N signaling remain elusive. Here, we report that a mutation of FERONIA (FER), a receptor kinase that plays versatile roles in plant cell growth and stress responses, caused hypersensitivity to a high C/N ratio in Arabidopsis.

Metabolomics Analysis Identifies Sphingolipids as Key Signaling Moieties in Appressorium Morphogenesis and Function in Magnaporthe oryzae.

The blast fungus initiates infection using a heavily melanized, dome-shaped infection structure known as the appressorium, which forcibly ruptures the cuticle to enter the rice leaf tissue. How this process takes place remains not fully understood. Here, we used untargeted metabolomics analyses to profile the metabolome of developing appressoria and identified significant changes in six key metabolic pathways, including early sphingolipid biosynthesis.