Low-temperature plasma efficiently promotes blood coagulation with less thermal injury in porcine models.

How to maintain the efficacy of blood coagulation while minimizing thermal damage has been a significant challenge in the optimization of clinical electrosurgical units (ESUs). Recent studies have reported that reactive species produced by low-temperature plasma (LTP) can promote blood coagulation. In this study, two forms of home-made atmospheric pressure plasma jets (APPJs) were compared to argon plasma coagulation (APC) and utilized in three different porcine bleeding models.

Transcriptomic Insights into Adaptive Strategies of Co-Producing KPC-2 and NDM-5 Carbapenemases Under Meropenem Stress.

Background: The emergence of strains that co-produce multiple carbapenemases poses significant threats to clinical management; however, the molecular adaptations driving their resilience remain poorly characterized.

Methods: This study aimed to investigate the resistance and adaptive mechanisms of the and co-producing KP4 strains. The antimicrobial susceptibility of KP4 was determined using Vitek, a carbapenemase inhibitor enhancement assay, and single-cell Raman spectroscopy.

Non-Gaussianity of neurotransmitters co-released from mammalian adrenal chromaffin cells.

While synaptic currents in computational neuroscience are conventionally modeled as Gaussian processes, there tends to be theoretical assumption that non-Gaussian Lévy processes can better describe the stochastic nature of neurotransmitter release in real neurophysiological scenarios. To support this view, we conduct statistical inference with the recordings of the co-release currents of two neurotransmitters from mammalian adrenal chromaffin cells by two steps. First, both the deterministic part and the random part of the current time series are separated by local weighted regression based on the individual vesicle releases and the entire co-release process, respectively.

Projections of Heat-Related Mortality in Chinese Cities: The Roles of Climate Change, Urbanization, Socioeconomic Adaptation, and Landscape-Level Strategies.

Background: Physiological heat strain induced by extreme temperatures in cities has led to significant heat-related deaths. Although socioeconomic adaptation is suggested to mitigate this issue, its effectiveness is limited. Conversely, there is a lack of comprehensive evaluation on the effectiveness of landscape-level strategies for mitigating heat-related deaths.

Accelerated detection of carbapenem resistance in Klebsiella pneumoniae via single-cell Raman spectroscopy.

Antibiotic resistance poses a significant global public health challenge, particularly concerning carbapenem-resistant Klebsiella pneumoniae (CRKP). A Raman-based antimicrobial susceptibility testing (R-AST) method was developed, utilizing D₂O-labeled single-cell Raman spectroscopy alongside the Metabolic Inhibition Level (MIL) for rapid CRKP antimicrobial susceptibility testing and carbapenemase enzyme typing. By analyzing 30,000 single-cell Raman spectra from 57 K.

The mitochondrial protectant SS31 optimized decellularized Wharton's jelly scaffold improves allogeneic chondrocyte implantation-mediated articular cartilage repair.

Background: The process of allogeneic chondrocyte implantation entails obtaining donor chondrocytes, culturing them in a medium enriched with growth factors, and then introducing them-either individually or in conjunction with biocompatible scaffolds-into areas of cartilage damage. While promising, this approach is hindered by mitochondrial dysfunction in the implanted chondrocytes.

Methods: This research introduced an innovative approach by creating a new type of scaffold derived from Decellularized Umbilical Cord Wharton's Jelly (DUCWJ) extracted from human umbilical cords.

Antibacterial and antibiofilm activities of star anise-cinnamon essential oil against multidrug-resistant Thompson.

Introduction: The emergence of foodborne multidrug-resistant (MDR) has attracted considerable global attention. Given that food is the primary transmission route, our study focuses on , a freshwater snail that is commonly consumed as a specialty food in Guangxi, China.

Methods: Eight MDR strains were isolated from samples collected across various markets.

Unveiling the Hidden Mechanism: How LuxS/AI-2 quorum sensing system drives antimicrobial resistance in Salmonella pullorum through activation of the efflux pump AcrAB-TolC.

Understanding the antimicrobial resistance (AMR) mechanism of Salmonella pullorum (SP), which is widespread among yellow chickens in China, is crucial for reducing significant economic losses in the industry. In this study, we explored the AMR mechanism by which the LuxS/AI-2-mediated quorum sensing (QS) system regulates the AcrAB-TolC efflux pump in the SP. The results showed that the luxS gene and the signaling molecule AI-2 had no effect on the growth of the SP strains.

Multi-Structure-Based Refractive Index Sensor and Its Application in Temperature Sensing.

In this paper, a new sensor structure is designed, which consists of a metal-insulator-metal (MIM) waveguide and a circular protrusion and a rectangular triangular cavity (CPRTC). The characterization of nanoscale sensors is considered using an approximate numerical method (finite element method). The simulation results show that the sharp asymmetric resonance generated by the interaction between the discrete narrow-band mode and the continuous wideband mode is called Fano resonance.

Effect of High-Energy Electron Beam Irradiation on the Structure and Thermoelectric Properties of Polypyrrole.

The effects of different doses (10-100 kGy) of electron beams on the molecular structure, microstructure, and thermoelectric properties of polypyrrole (PPy) under high-energy electron beam irradiation (10 MeV) were studied. The results showed that after electron beam irradiation, the conductivity of PPy increased slightly, but the Seebeck coefficient and power factor remained relatively stable. The structural analysis of FTIR, Raman spectroscopy, and X-ray diffraction indicated that the molecular structure of PPy was strongly stable, and its microstructure was only slightly affected by electron beam irradiation.

Developmental dynamics mimicking inversely engineered pericellular matrix for articular cartilage regeneration.

The mechanical mismatch of scaffold matrix-mesenchymal stem cells (MSCs) has been a longstanding issue in the clinical application of MSC-based therapy for articular cartilage (AC) regeneration. Existing tissue-engineered scaffolds underestimate the importance of the natural chondrocyte pericellular matrix (PCM). Here, we reveal the temporal and spatial characteristics of collagen distribution around the chondrocytes.

The complete chloroplast genome of and comparative analysis with related species from Magnoliaceae and Illiciaceae.

(Illiciaceae), an ecologically significant endemic plant, predominantly grows in Guangxi, China, which is the primary region for its cultivation. This area accounts for more than 80% of the total cultivation and yield in China. Despite its importance, comprehensive studies on the chloroplast (cp) genome of are limited.

Multi-Cavity Nanorefractive Index Sensor Based on MIM Waveguide.

Within this manuscript, we provide a novel Fano resonance-driven micro-nanosensor. Its primary structural components are a metal-insulator-metal (MIM) waveguide, a shield with three disks, and a T-shaped cavity (STDTC). The finite element approach was used to study the gadget in theory.

Alarin regulates RyR2 and SERCA2 to improve cardiac function in heart failure with preserved ejection fraction.

Heart failure with preserved ejection fraction (HFpEF), a complex disease that is increasingly prevalent due to population aging, pose significant challenges in its treatment. The present study utilized the HFpEF rat model and H9C2 cells as research subjects to thoroughly investigate the potential mechanisms of alarin in protecting cardiac function in HFpEF. The study shows that under HFpEF conditions, oxidative stress significantly increases, leading to myocardial structural damage and dysfunction of calcium ion channels, which ultimately impairs diastolic function.

The role of hematological parameters in asymptomatic and non-severe cases of Omicron variant infection.

Background: Omicron variants are currently the predominant circulating lineage worldwide and most cases are mild or asymptomatic. The Omicron variant is characterized by high transmissibility and immune evasion. Early identification of Omicron cases in clinical settings is crucial for controlling its spread.

Enhanced n-Type Thermoelectric Properties and Structure Evolution of Carbonized Metal-Coordination Polydopamine.

Carbonized polydopamine (cPDA) exhibits a nitrogenous graphite-like structure with n-type semiconductor property. However, the low electrical conductivity and Seebeck coefficient of cPDA cannot meet the needs of flexible thermoelectric devices. In this study, a series of metal ions were coordinated with cPDA to enhance n-type thermoelectric properties.

Magnetic multilayer hydrogel oral microrobots for digestive tract treatment.

Oral administration is a convenient drug delivery method in our daily lives. However, it remains a challenge to achieve precise target delivery and ensure the efficacy of medications in extreme environments within the digestive system with complex environments. This paper proposes an oral multilayer magnetic hydrogel microrobot for targeted delivery and on-demand release driven by a gradient magnetic field.

PML::RARA and GATA2 proteins interact via DNA templates to induce aberrant self-renewal in mouse and human hematopoietic cells.

The underlying mechanism(s) by which the PML::RARA fusion protein initiates acute promyelocytic leukemia is not yet clear. We defined the genomic binding sites of PML::RARA in primary mouse and human hematopoietic progenitor cells with V5-tagged PML::RARA, using anti-V5-PML::RARA chromatin immunoprecipitation sequencing and CUT&RUN approaches. Most genomic PML::RARA binding sites were found in regions that were already chromatin-accessible (defined by ATAC-seq) in unmanipulated, wild-type promyelocytes, suggesting that these regions are "open" prior to PML::RARA expression.

[ Subtype Allele Identification and Protein Structure Analysis in Newborns].

Objective: To study the serological characteristics of subtype and explore its genetic molecular mechanism.

Methods: ABO blood group identification was performed on proband and her family members by routine serological methods. ABO genotyping and sequence analysis were performed by polymerase chain reaction-sequence specific primer (PCR-SSP), and direct sequencing of PCR products from exons 6 and 7 of gene were directly sequenced and analyzed.

Proteogenomic analysis reveals cytoplasmic sequestration of RUNX1 by the acute myeloid leukemia-initiating CBFB::MYH11 oncofusion protein.

Several canonical translocations produce oncofusion genes that can initiate acute myeloid leukemia (AML). Although each translocation is associated with unique features, the mechanisms responsible remain unclear. While proteins interacting with each oncofusion are known to be relevant for how they act, these interactions have not yet been systematically defined.

Immunotherapy combined with local therapy in the late-line treatment of repair-proficient (pMMR)/microsatellite stable (MSS) metastatic colorectal cancer.

Colorectal cancer (CRC) is one of the most common malignancies, and at the initial visit, most patients are diagnosed with metastatic CRC (mCRC). However, immunotherapy is only and highly effective in a very small proportion of patients with mCRC having mismatch repair defect (dMMR)/high microsatellite instability, and the majority of the patients with mCRC having mismatch repair proficient (pMMR)/microsatellite stability (MSS) cannot benefit from it. At present, many clinical studies of immunotherapy combined with tyrosine kinase inhibitors (TKIs) are trying to regulate the immune microenvironment of pMMR/MSS mCRC, transforming a "cold tumor" into a "hot tumor," which has not only surprising effects but also certain limitations, i.

Investigation of biofilm formation and the associated genes in multidrug-resistant in China (2018-2022).

The study explored the biofilm (BF) formation capacity, BF-related gene profiles, and the trends in antimicrobial resistance (AMR) of (SP) strains over several years. A total of 627 SP strains were isolated from 4,540 samples collected from chicken farms in Guangxi, China during 2018-2022. The BF-forming capacity of these isolates was assessed using crystal violet staining, and the presence of eight BF-related genes (, and ) in BF formation-positive strains was determined through Polymerase Chain Reaction (PCR) analysis.