The Femoral Neck-Shaft Offset: A Key Element in the Reconstruction of Intertrochanteric Fracture.

Background: Anteromedial cortex reduction and accurate placement of the cephalomedullary nail is the key point to confront implant failure of intertrochanteric fractures. Existing intramedullary nails cannot compensate for femoral neck-shaft offset (FNSO), potentially undermining surgical outcome. This study aimed to investigate the effect of FNSO on anteromedial cortex reduction and accurate placement of the cephalomedullary nail for intertrochanteric fractures.

Schisandrol A-loaded mesenchymal stem cell exosomes modulate FTO/miR-21/WNT5A axis to promote skin healing.

Background: Schisandrol A (SA), the primary active component of Schisandra chinensis, exhibits antioxidant properties, safeguarding cells from oxidative damage. However, its effect on wound healing remains elusive, and its low water solubility restricts its utilization efficiency.

Objective: This study aimed to explore the impact of SA on HaCaT cell proliferation and migration through in vitro experiments, thereby providing deeper insights into its role in skin wound healing.

Changes of microbial life history strategies to soil nutrient limitations following vegetation restoration and its impact on carbon utilization efficiency.

Vegetation restoration often leads to changes in soil nutrients, prompting adjustments in microbial growth strategies and compositions. However, how microbial adaptive strategies affect microbial carbon use efficiency (CUE) remains unclear. This study utilized the space-for-time substitution method and involved three forest types in the Taiyi Mountain area as research objects for vegetation restoration: Platycladus orientalis (L.

Single-nucleotide Resolution Epitranscriptomic Profiling Uncovers Dynamic mA Regulation in Bovine Preimplantation Development.

RNA -methyladenosine (mA) plays a crucial role in regulating gene expression during early embryonic development. However, the mA dynamics at single-nucleotide resolution in preimplantation development remain uncharacterized, and the functional significance of site specific mA modifications in key developmental regulators is largely unknown. Here, using SAC-seq, a single-base resolution, antibody-independent mA profiling method, we generate the first comprehensive mA landscape in bovine oocytes and preimplantation embryos.

The Role of Bedtime Procrastination, Rumination, Loneliness, and Positive Body Image in Predicting Sleep Quality Among University Students: A Sex-Specific Analysis.

Objective: This study aimed to analyze the impact of bedtime procrastination, rumination, loneliness, and positive body image on university students' sleep quality, and to explore potential mediating pathways and sex differences.

Methods: A total of 674 students from a university in southern China were recruited. Assessments of participants' body measurements were conducted, followed by the completion of a general information questionnaire, Pittsburgh Sleep Quality Index, Bedtime Procrastination Scale, Body Appreciation Scale, Body Image-Acceptance and Action Questionnaire, Ruminative Responses Scale, and University of Loneliness Scale.

Unanticipated Global Emergence of Multiple Mutants Selected by Mycophenolic Acid Driving Increasing Outbreaks in Solid Organ Transplant Recipients.

Background: Classified by the WHO as one of the 19 most dangerous fungal pathogens, has been associated with increasing outbreaks of pneumonia (PCP) among solid organ transplant (SOT) recipients worldwide. Mycophenolic acid (MPA), an inosine monophosphate dehydrogenase (IMPDH) inhibitor commonly used as an immunosuppressant to prevent organ rejection, is a risk factor for PCP. However, MPA also displays antifungal activity, potentially protecting against PCP, despite not being used to treat it.

A head-to-head NLR gene pair from wild emmer confers stripe rust resistance in wheat.

Wheat stripe rust poses a major threat to global food security. Discovery of disease resistance genes from wild relatives enables multigene stacking that could enhance durability. Here we use map-based cloning and long-read sequencing to isolate two adjacent nucleotide-binding and leucine-rich repeat (NLR) receptors from wild emmer wheat.

Superhydrophobic ROS biocatalytic metal coatings for the rapid healing of diabetic wounds.

In diabetic patients, hyperglycemia-induced elevated reactive oxygen species (ROS) accumulation severely impairs chronic wound healing by causing cellular component oxidation, inducing DNA damage, triggering cell death, exacerbating inflammatory responses, disrupting vascular endothelial function, reducing local blood supply, and inhibiting angiogenesis. This cascade results in a vicious cycle that delays the healing process. In this study, we developed a novel multifunctional composite dressing by depositing a transition-metal catalytic coating onto a superhydrophobic polydimethylsiloxane layer via magnetron sputtering.

A Universal 6iL/E4 Culture System for Deriving and Maintaining Embryonic Stem Cells Across Mammalian Species.

The derivation of authentic embryonic stem cells (ESCs) from diverse mammalian species offers valuable opportunities for advancing regenerative medicine, studying developmental biology, and enabling species conservation. Here, we report the development of a robust, serum-free culture system, termed 6iL/E4 that enables the derivation and long-term self-renewal of ESCs from multiple mammalian species, including mouse, rat, bovine, rabbit, and human. Using systematic signaling pathway analysis, we identified key regulators-including GSK3α, STAT3, PDGFR, BRAF, and LATS-critical for ESC maintenance across species.

Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation.

Extraembryonic endoderm stem cells (XENs) resemble the primitive endoderm of blastocyst and provide valuable alternatives for understanding hypoblast development and building stem cell-based embryo models. Here, we report that a chemical cocktail (FGF4, BMP4, IL-6, XAV939, and A83-01) enables the de novo derivation and long-term culture of bovine XENs (bXENs) from blastocysts. Transcriptomic and epigenomic analyses confirm the identity of bXENs and reveal that bXENs resemble the hypoblast lineages of early bovine peri-implantation embryos.

Emergence and transmission of Carbapenem-resistant gene in healthy pigs in Baise, Guangxi, China: a discovery.

A total of 366 tonsillar tissue samples were collected from healthy free-range pigs owned by farmers across 12 districts in Baise City, Guangxi, China. This initiative successfully isolated six strains of Carbapenem-Resistant (CRE), including four strains of , and one of and . Assessments utilizing Carbapenemase inhibitor enhancement test, in conjunction with the ResFinder resistance gene database, revealed that five strains carried the gene, while one strain possessed the gene.

Projections of Heat-Related Mortality in Chinese Cities: The Roles of Climate Change, Urbanization, Socioeconomic Adaptation, and Landscape-Level Strategies.

Background: Physiological heat strain induced by extreme temperatures in cities has led to significant heat-related deaths. Although socioeconomic adaptation is suggested to mitigate this issue, its effectiveness is limited. Conversely, there is a lack of comprehensive evaluation on the effectiveness of landscape-level strategies for mitigating heat-related deaths.

Unravelling consensus genomic regions conferring stem-related traits in wheat via meta-QTL analysis.

Background: Wheat stem is an important vegetative organ, that is responsible for supporting leaves and spikes, and transporting water, minerals and organic nutrients to spikes through vascular bundles, thereby promoting plant growth and development. Therefore, wheat stems contribute to plant structure and total yield.

Results: We constructed a high-density consensus map and performed a meta-analysis using 306 stem-related QTLs.

Exploring exercise, emotional eating, and body dissatisfaction in depression risk: Using structural equation modeling.

In order to fill the relevant research gap on the influence of body dissatisfaction on depression in Chinese college students, we used structural equation modeling to explore the relationships between physical activity, body dissatisfaction, eating behavior, and depression. Participants were 1,714 undergraduate students in Southern China. Exercise served as a significant mediator between body mass index and depression, as well as between body dissatisfaction and emotional eating, accounting for 30% and 19.

Organoid-Guided Precision Medicine: From Bench to Bedside.

Organoid technology, as an emerging field within biotechnology, has demonstrated transformative potential in advancing precision medicine. This review systematically outlines the translational trajectory of organoids from bench to bedside, emphasizing their construction methodologies, key regulatory factors, and multifaceted applications in personalized healthcare. By recapitulating physiological architectures and disease phenotypes through three-dimensional culture systems, organoids leverage natural and synthetic scaffolds, stem cell sources, and spatiotemporal cytokine regulation to model tissue-specific microenvironments.

Galangin exosomes induce cell apoptosis through miR-10b/P53 axis in gastric cancer.

Galangin is an extract of traditional Chinese medicine with anti-cancer effect. Exosomes (Exo) contain RNA, peptides, or proteins and communicate between cells through a bio-genesis pathway. In the present study, Gastric cancer (GC) cell exosomes containing galangin were obtained.

The associations between BMI, body appreciation, body image flexibility, sleep duration and loneliness in workers in southern China.

Background: Loneliness is a growing public health problem worldwide that can affect mental and emotional health and increase mortality. This study examined the relationship between body mass index (BMI), body image flexibility, body appreciation, and loneliness among Chinese workers, aiming to propose effective measures for reducing loneliness and promoting mental health.

Methods: A total of 464 workers were recruited for this study.

The effects of body dissatisfaction, lifestyle, and loneliness on emotional eating among older adults in Northeast China.

Objectives: Emotional eating is a psychological aspect of eating disorders. Recent studies have found that emotional eating is prevalent among older adults. This study aimed to investigate the current situation of emotional eating and explore the factors influencing emotional eating in older adults.

Exploring the relations between body dissatisfaction, rumination, physical activity, and restrained eating: a latent moderated structural equation approach.

Background: Previous studies have shown that body dissatisfaction is closely related to restrained eating, however, the specific mechanisms and conditions of body dissatisfaction need to be further explored. Therefore, this study aims to explore the mediating and moderating variables influencing the relations between body dissatisfaction and restrained eating, providing recommendations for the prevention of restrained eating among university students.

Methods: Participants comprised 1213 university students who completed the Negative Physical Self Scale, Ruminative Responses Scale, Restrained Eating Scale, and Physical Activity Scale.

Biosynthesized Fe-C-dots nanozymes modulate growth, physiological and phytochemical peculiarity to improve saline-alkaline stress tolerance in wheat.

Nanotechnology has shown great potential to improve agricultural production and increase crop tolerance to abiotic stresses, including saline-alkaline environments. This study focuses on the biological mechanism of biocompatible iron-doped carbon dots (Fe-C-dots) nanozyme biosynthesized from artemisinin extract to alleviate saline-alkaline stress in wheat (Triticum aestivum L.).

Pollution and health risk assessment of rare earth elements in soil, water and vegetables from an ion-adsorption rare earth mining area in Ganzhou, China.

Rare earth elements (REEs) are increasingly recognized as significant environmental pollutants due to their environmental persistence, bioaccumulation, and chronic toxicity. This study assessed REEs pollution in soil, water, and vegetables in an ion-adsorption rare earth mining area in Ganzhou, and evaluated the associated health risks to the local population. Results indicated that the REEs content in soil ranged from 168.

Exercise addiction in college students: the impact of body dissatisfaction, stress, physical activity and gender.

Introduction: Engaging in physical activity is commonly regarded as beneficial to health. However, exercise addiction may arise when enthusiasm for exercise reaches a level that disrupts life balance and overall well-being. Factors influencing college students' exercise addiction remain largely unknown.

Adipose-derived stem cells attenuate rheumatoid arthritis by restoring CXCR1 synovial lining macrophage barrier.

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease and the integrity of CXCR1 synovial macrophage barrier significantly impacts its progression. However, the mechanisms driving the dynamic changes of this macrophage barrier remain unclear. Traditional drug therapies for RA have substantial limitations.

[An experimental study on distal locking of femoral intramedullary nail assisted by an intelligent orthopedic robot].

Objective: To explore the advantages and effectiveness of the independently developed intelligent orthopedic robot-assisted distal locking of femoral intramedullary nails.

Methods: Thirty-two adult cadaveric femur specimens were randomly divided into two groups, with 16 specimens in each group. The experimental group used the intelligent orthopedic robot to assist in the distal locking of femoral intramedullary nail holes, while the control group used the traditional method of manual locking under X-ray fluoroscopy.

Development and characterization of small translocations between the Pm13 introgression from Aegilops longissima and wheat homoeologous A, B and D chromosomes.

Background: Wild relatives have substantial impacts on the resistance of wheat to biotic and abiotic stresses. The genetic diversity of these wild varieties can be employed to widen the wheat gene pool by introducing wild allele introgression and genome structure variations. The powdery mildew resistance gene Pm13 was derived from the wheat wild relative species Aegilops longissima (SS, 2n = 2x = 14) and was transferred into wheat chromosome 3BS over 30 years.