Genomic and transcriptomic data reveal molecular differences between homologous recombination deficiency subgroups in Chinese ovarian cancer patients.

Background: Ovarian cancer (OV) has the highest mortality rate among gynecological cancers and shows varied responses to chemotherapy combined with PARP inhibitors based on homologous recombination deficiency (HRD) subtypes.

Methods: This study enrolled 143 Chinese OV patients to determine the HRD score grouping threshold using genomic features, dividing patients into HRD-high and HRD-low groups. Multi-omics sequencing was conducted on 70 patients receiving adjuvant chemotherapy with PARP inhibitors.

A strategy for challenging tumorous bone regeneration by borosilicate bioactive glass boosting moderate magnetic hyperthermia.

Osteosarcoma (OS), with a high tendency for recurrence and metastasis, is associated with severe impairment of bone regeneration. The inherent temperature-sensitive property of tumors positions magnetic hyperthermia (MH) as an increasingly significant area in non-pharmacological cancer treatments. However, the temperature threshold for tumor ablation often causes tissue damage and bone homeostasis imbalance.

Application of Serum sFlt-1/PlGF Ratio Combined with Uterine Artery Blood Flow Ultrasound in Predicting Early-Onset Preeclampsia.

Objective: This study aimed to evaluate the predictive efficacy of combining serum soluble fms-like tyrosine kinase-1/placental growth factor (sFlt-1/PlGF) ratio with uterine artery Doppler ultrasound for early-onset preeclampsia (PE) before 34 weeks' gestation.

Methods: A retrospective cohort analysis was conducted on 148 singleton pregnancies (control group: n = 76; early-onset PE group: n = 72) who delivered at our institution between July 2023 and June 2024. At 24-28 weeks, serum sFlt-1 and PlGF levels were quantified via electrochemiluminescence immunoassay (Roche Elecsys), and uterine artery pulsatility index (PI), resistance index (RI), and systolic/diastolic ratio (S/D) were measured by Doppler ultrasound (M22 system, standardized settings).

Hydroxyapatite injectable hydrogel with nanozyme activity for improved immunoregulation microenvironment and accelerated osteochondral defects repair via mild photothermal therapy.

Osteochondral defect repair presents a significant clinical challenge due to the immune microenvironment at the injury site, which impairs osteogenesis. In this study, a Ce/MnHAp was developed by integrating it into an HES hydrogel. This bi-layered hydrogel system comprises an upper cartilage-mimicking layer composed of pure hydrogel and a lower subchondral bone-mimicking layer containing Ce/MnHAp.

Research on the diagnosis model of osteoarthritis based on methylation-related genes using machine learning algorithms.

Objective: To construct a diagnostic model of osteoarthritis related to methylation genes using machine learning algorithms, and analyze its prognostic value and biological functions.

Methods: The GSE 63695 and GSE162484 datasets including human osteoarthritis (OA) and normal samples were downloaded from the GEO datasets. The microarray chip data of chondrocytes were analyzed using R software to obtain differentially methylated genes.

Potential use of plasma-activated water cleaning for aflatoxin B degradation in peanuts.

Aflatoxin B (AFB) is a prevalent mycotoxin in oil crops, such as peanuts, with potent teratogenic and carcinogenic properties, posing a threat to human health. Plasma-activated water (PAW) with abundant active species is an emerging food decontamination technology. Herein, this study investigated the degradation efficiency and products of PAW on AFB, as well as the peanut quality.

Sensitive enzymatic cycling assay for glutathione and glutathione disulfide content.

Background: It is crucial to develop reliable techniques for accurately detecting and measuring glutathione (GSH) and glutathione disulfide (GSSG) content in biological samples. However, there is currently not yet a full consensus on the best approach for preparing and analyzing samples to obtain accurate quantitative results.

Results: This study screened N-ethylmaleimide (NEM) as a masking agent and 5-sulfosalicylic acid (SSA) as a protein removal agent to establish a standard and stable method for GSH and GSSG determination.

CAR19-T/NK cell and circular aptamer-drug conjugate (ApDC) combination treatment increases immunotherapy efficacy.

Chimeric antigen receptor (CAR)-based cell therapies have transformed the treatment of haematological malignancies, especially acute lymphoblastic leukaemia (ALL). However, drug resistance limits long-term efficacy. This study aimed to develop a novel combination therapy using aptamer‒drug conjugates and CAR19-T/natural killer (NK) cells to eliminate tumour cells completely and improve the efficacy of CAR-based cell therapies.

On Patient Quality of Life: Impacts of Knee Osteoarthritis on Pain, Anxiety, Depression, Fatigue and Sleep Disorders.

Aim: Patients repeatedly experiencing a sustained disease course, recurrent chronic pain, and serious sleep disorder will weaken their physical resistance and self-care ability. Further evidence on patients with knee osteoarthritis (KOA) and related latent classes (LCs) of symptom clusters is needed in order to clarify its significance for symptom management and quality of life (QoL) enhancement.

Design: This study aimed to generate evidence on differences in symptoms experienced by patients with knee osteoarthritis, identify related LCs, and explore the differences of each LC by demographic, disease, individual characteristics, and QoL.

Atomically Engineered Acridine Derivatives Serve as Metal-Free and Self-Sensitized Catalysts for Solar-Driven CO to Formic Acid with High-Efficiency and Near-Perfect Selectivity.

Achieving efficient and selective light-driven CO conversion to formic acid is a significant scientific challenge, particularly when utilizing purely organic, metal-free, and earth-abundant element-based molecule photocatalysts. Herein, we first reported the discovery of acridine derivatives (DADN, PXZN, and PTZN) as new-type, metal-free, self-sensitized molecule catalysts that enabled exceptional performance in solar-driven CO reduction to formic acid. Notably, the atomically engineered sulfur-containing heterocycle PTZN demonstrated unprecedented formate yield rate of 47.

Initial stiffness of integrally-formed GFRP T-joint in GFRP transmission tower.

It is recognized that the semi-rigidity of the glass fiber reinforced polymer (GFRP) joint should be considered in the structural analysis of GFRP structures, because of the relatively small initial stiffness resulting from the intrinsic property of GFRP material. Regarding the integrally-formed GFRP T-joint in the GFRP transmission tower, the study of its semi-rigidity remains inadequate. This paper aims to develop the relevant method for approximating the initial stiffness of the integrally-formed GFRP T-joint, in which the joint connects a vertical column with circular hollow section (CHS) and a horizontal bracing beam with rectangle section (RS).

Correlation between excessive sugar intake and overactive bladder in children.

Purpose: The aim of this study was to explore the relationship between excessive sugar intake and symptoms of paediatric overactive bladder (OAB).

Methods: Clinical data was collected from 97 patients diagnosed with OAB at our hospital between July 2024 and January 2025. The relationships between sugar intake and OAB symptom scores were analysed.

Circulating-Water Mattress Enhances Thermal Comfort and Patient Satisfaction but Shows Non-Superiority in Temperature Maintenance in Ophthalmic Day-Case Surgery: A Randomized Controlled Trial.

Purpose: Perioperative hypothermia, a common complication of general anesthesia, is associated with adverse outcomes. While active warming methods are recommended, the effectiveness of circulating-water mattresses during ophthalmic surgeries remains understudied. This randomized controlled trial assessed whether a circulating-water mattress combined with a cotton quilt (Group W) was superior to a cotton quilt alone (Group C) in maintaining patient body temperature during ophthalmic day-case surgery.

Microfluidic-Enabled Production of DNA Barcoded APC Library (MEDAL) for High Throughput T Cell Epitope Screening.

Screening for peptide fragments that can be displayed on antigen-presenting cells is an essential step in vaccine development. The current approach for this process is slow and costly as it involves separately pulsing cells with chemically synthesized peptides. This study presents Microfluidic-Enabled production of DNA-barcoded APC Library (MEDAL), a high throughput microfluidic droplet platform for parallel production of DNA-barcoded Antigen Presenting Cells (APCs) loaded with enzymatically synthesized peptides.

The Halo Vest Immobilization for Craniocervical Junction Tuberculosis: A Comparison of Treatment Options.

Objective: The treatment of tuberculosis at the craniovertebral junction (CVJ) remains challenging, with significant debate surrounding therapeutic approaches. Halo vest (HV) therapy provides a non-invasive immobilization alternative, while occipitocervical fusion (OCF) offers a surgical option. However, limited evidence exists comparing the efficacy of HV therapy with OCF for CVJ tuberculosis.

Risk Factors for Pulmonary Embolism After Head and Neck Cancers Resection: A Retrospective Case Control Study.

Objective: Pulmonary embolism (PE) after surgery is a severe thrombotic event for patients suffering from head and neck malignancies. Our study analyzed the factors related to PE in such patients following radical resection. A case-control study was designed to retrospectively investigate the factors associated with the increased risk.

Monocular depth estimation via a detail semantic collaborative network for indoor scenes.

Monocular image depth estimation is crucial for indoor scene reconstruction, and it plays a significant role in optimizing building energy efficiency, indoor environment modeling, and smart space design. However, the small depth variability of indoor scenes leads to weakly distinguishable detail features. Meanwhile, there are diverse types of indoor objects, and the expression of the correlation among different objects is complicated.

Does income have a non-linear impact on residents' BMI? Re-examining the obesity Kuznets curve.

Background: After more than 40 years of growth, the income of Chinese residents has greatly increased; however, the problems of overweight and obesity among residents have become increasingly prominent.

Methods: We used data from the China Family Panel Studies (CFPS) to study the relationship between residents' income and obesity using the instrumental variable (IV) method.

Results: The impact of income on residents' body mass index (BMI) is an inverted U-shape; that is, when income is low, BMI significantly rises with income, and when per capita income exceeds 57,066 yuan in 2023 prices (equivalent to 8,098 dollars), further increases in income will lead to a decrease in BMI.

Corilagin enhances wound healing by modulating the macrophage phenotype in diabetic mice.

Excessive inflammation is a prominent issue in diabetic wounds, leading to delayed healing or amputation. Corilagin (Cori) is a natural polyphenolic compound with diverse pharmacological activities, particularly its anti-inflammatory properties. The aim of this study was to evaluate the anti-inflammatory effect of Cori on diabetic wounds and to explore the potential underlying mechanisms.

Prevalence of Japanese encephalitis in pigs in Mainland China during 2000-2024: a systemic review and meta-analysis.

Background: Japanese encephalitis (JE) is an acute viral disease transmitted mainly by mosquitoes, primarily affecting Southeast Asia, and the Western Pacific. This study aimed to analyze the factors contributing to JE occurrence in pigs across China.

Methods: A systematic search was done using six databases for the published epidemiological studies on porcine JE, including the Chinese Web of Knowledge (CNKI), Wan Fang Database, ScienceDirect, Web of Science, VIP Chinese Journal Database, and PubMed.

Carbon black imprinted o-diaminobenzene sensing platform for sensitive electrochemical detection of interleukin 6.

As a typical proinflammatory cytokine, interleukin 6 (IL-6) exhibits great influence on treating diseases (e.g., urocystitis) and adjusting immune.

[An experimental study on distal locking of femoral intramedullary nail assisted by an intelligent orthopedic robot].

Objective: To explore the advantages and effectiveness of the independently developed intelligent orthopedic robot-assisted distal locking of femoral intramedullary nails.

Methods: Thirty-two adult cadaveric femur specimens were randomly divided into two groups, with 16 specimens in each group. The experimental group used the intelligent orthopedic robot to assist in the distal locking of femoral intramedullary nail holes, while the control group used the traditional method of manual locking under X-ray fluoroscopy.

Study on the expression of S100A4 and HMGA1 in endometrial carcinoma and their correlation with metastasis.

Objectives: To investigate the relationship between the expression of S100 calcium-binding protein A4 (S100A4), high mobility group protein A1 (HMGA1) and clinicopathological features, as well as postoperative recurrence and metastasis in endometrial cancer patients.

Methods: Sixty endometrial cancer patients (observation group) were selected for this study, along with 40 patients who underwent hysterectomy for benign diseases (control group) during the same period. Surgically resected endometrial cancer tissues and normal endometrial tissues were collected.

Umbilical Cord Mesenchymal Stem Cells Could Reduce Lung Damage Caused by H1N1 Influenza Virus Infection.

Influenza A virus infection can cause acute respiratory distress syndrome (ARDS), and to date, viral pneumonia has been the main cause of ARDS. Bone marrow mesenchymal stem cells have shown promise for treating lung injury caused by avian influenza virus infection. At present, studies of the use of other stem cell types to treat human influenza virus-mediated lung damage are sparse.