The role of lipid metabolism disorder in the progression and treatment of ocular vascular diseases.

Lipid metabolism plays a critical role in maintaining normal physiological functions and is strongly linked to the pathogenesis of ocular vascular diseases. This review examines how disorders of lipid metabolism drive progression in ocular vascular diseases, including diabetic retinopathy, age-related macular degeneration, retinal vascular occlusive diseases, and retinopathy of prematurity. These disorders are classified as a related group due to their common feature of impaired ocular vascularization.

Weakly hydrophobic antibiotics leaching in an alpine soil of the Tibetan Plateau in responding to macropore flow.

In alpine meadow regions, macropore flow is a critical but inadequately understood pathway for antibiotic transport. The complex relationship between macropore structure, flow dynamics, and solute properties presents a significant research gap. Methodological limitations hinder the accurate characterization of solute migration mechanisms due to complex macropore structures.

Emerging opportunities and challenges in small molecule development for non-HSP90 chaperones.

Heat shock proteins (HSPs) are pivotal regulators of proteostasis, with their dysregulation implicated in cancer, neurodegeneration, and infectious diseases. Significant progress has been made in targeting HSP90, particularly in oncology, where inhibitors have demonstrated considerable therapeutic potential and validated HSP90 as a promising drug target. However, other HSP families remain relatively underexplored as drug targets despite their critical biological roles.

Novel ternary deep eutectic solvent coupled with in-situ-ultrasound synergistic extraction of flavonoids from Epimedium wushanense: machine learning, mechanistic investigation, and antioxidant activity.

This study innovatively developed a novel ternary deep eutectic solvent coupled with in-situ-ultrasound synergistic extraction (TDES-ISUSE)method for efficient extraction of total flavonoids (TF) fromEpimedium wushanense. Among 20 prepared DESs, the ternary system composed of choline bitartrate, urea, and glycerol (Chb:Ure:Gly) exhibited the highest extraction efficiency. Response surface methodology-artificial neural network-genetic algorithm (RSM-ANN-GA) optimization identified the optimal extraction parameters: water content of 32 %, vortex time of 10 min, liquid/solid ratio of 34:1 mL/g, ultrasound time of 30 min, and ultrasound power of 270 W.

Engineered Exosomes Complexed with Botulinum Toxin Type A for Enhanced Anti-Aging Effects on Skin.

Skin aging is commonly characterized by increased wrinkles, loss of elasticity, and hyperpigmentation, significantly affecting personal appearance and quality of life. Although botulinum toxin type A (BTX-A) has been widely applied in cosmetic anti-wrinkle treatments, its intrinsic cytotoxicity limits broader clinical applications. In this study, we developed a novel exosome-based BTX-A composite delivery system designed to synergize the anti-aging properties of exosomes with the wrinkle-reducing effects of BTX-A while reducing toxicity.

Citric Acid and Polyethylene glycol Modified Na-Cyclodextrin MOFs for Efficient Antibiotic Removal and In-Depth Investigation of Adsorption Mechanisms.

Antibiotics in wastewater have a significant threat to human health and ecosystems. Metal-organic frameworks (MOFs) are among the most promising adsorbents for removing antibiotics from wastewater. In the present study, an environmentally friendly sodium-based cyclodextrin metal organic framework (CD-MOF) was synthesized and further modified with citric acid (CA) and polyethylene glycol (PEG) to enhance its water stability and adsorption capacity.

Progress and trends on machine learning in proteomics during 1997-2024: a bibliometric analysis.

Objective: Despite growing interest in the application of machine learning (ML) in proteomics, a comprehensive and systematic mapping of this research domain has been lacking. This study addresses this gap by conducting the first large-scale bibliometric analysis focused exclusively on ML-driven proteomics, aiming to elucidate its knowledge structure, development trajectory, and emerging research trends.

Methods: A total of 5,156 publications from the Web of Science Core Collection (1997-2024) were retrieved and analyzed.

Enhanced diagnostic performance of WE-SHARP-DWI compared with SPAIR-DWI for focal liver lesion evaluation at 3T.

Purpose: This study aimed to compare the diagnostic performance of 2 fat suppression techniques in diffusion-weighted imaging (DWI) for detecting and assessing focal liver lesions (FLLs): water excitation spectral heterogeneity adaptive radiofrequency pulses (WE-SHARP) and conventional spectral adiabatic inversion recovery (SPAIR).

Materials And Methods: This prospective study enrolled eligible participants between October 2023 and August 2024. Various DWI techniques at 3T, SPAIR-DWI, WE-SHARP-DWI, and WE-SHARP-DWI with correction algorithms (WE-SHARP-DWI*), acquired at b values of 50, 400, 800, and 1200 s/mm², were used to evaluate FLLs.

Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis.

Biofluid flow generates fluid shear stress (FSS), a mechanical force widely present in the tissue microenvironment. How brain tumour growth alters the conduit of biofluid and impacts FSS-regulated cancer progression is unknown. Dissemination of medulloblastoma (MB) cells into the cerebrospinal fluid initiates metastasis within the central nervous system.

Efficient Synthesis of Protein-Polymer Conjugates with Open-Air Fabrication and Facile Purification Driven by the Thermoresponsive Protein-Polymer Conjugate.

A simple, efficient, and broadly applicable method for the preparation of protein-polymer conjugates is of critical importance for their further development. However, existing approaches are often limited by challenges such as insufficient deoxygenation, complex purification processes, and poor compatibility of monomers and proteins. Herein, we present a comprehensive strategy for the synthesis of protein-polymer conjugates utilizing a thermosensitive, recyclable glucose oxidase-poly-(di-(ethylene glycol) methyl ether methacrylate) conjugate (GOX-PDEGMA) as part of the deoxygenation system.

Efficient Copper Biosorption by sp. ZC255: Strain Characterization, Kinetic-Equilibrium Analysis, and Genomic Perspectives.

Heavy metal pollution, particularly copper contamination, threatens the ecological environment and human survival. In response to this pressing environmental issue, the development of innovative remediation strategies has become imperative. Bioremediation technology is characterized by remarkable advantages, including its ecological friendliness, cost-effectiveness, and operational efficiency.

Integrated Transcriptomic and Metabolomic Analyses Shed Light on the Regulation of Aromatic Amino Acid Biosynthesis in a Novel Albino Tea () Mutation.

Off-white or yellowish shoots are common in tea plants ( L.), and such albino variations are often accompanied by metabolic reprogramming, including increased contents of amino acids and lower levels of polyphenols. Nonetheless, the molecular mechanisms that underlie these albino variations remain to be fully clarified.

Constructing a norm for children's scientific drawing: Distribution features based on semantic similarity of large language models.

The use of children's drawings to examining their conceptual understanding has been proven to be an effective method, but there are two major problems with previous research: (i) The content of the drawings heavily relies on the task, and the ecological validity of the conclusions is low. (ii) The interpretation of drawings relies too much on the subjective feelings of the researchers. To address this issue, this study uses the Large Language Model (LLM) to identify 1420 children's scientific drawings (covering nine scientific themes/concepts) and uses the word2vec algorithm to calculate their semantic similarity.

MoMtg1 Acts as a Novel Transcriptional Repressor of MoSwi6 During Appressorium-Mediated Penetration in the Rice Blast Fungus.

Appressoria are specialized penetration structures for many plant pathogenic fungi, including the rice blast fungus Magnaporthe oryzae, which evolves a set of complicated regulatory mechanisms to control appressorium development and function. Cell cycle control is essential for appressorium-mediated penetration, but the mechanism underlying its role remains largely elusive. Here, a conserved protein MoMtg1 is identified in filamentous fungi as a novel transcriptional repressor that plays a crucial role in cell cycle regulation.

encapsulation of coumarin/fluorescent bleacher/carbazole derivatives@Eu MOF materials for white light-emitting diodes.

Metal-organic frameworks (MOFs), characterized by their high porosity, large specific surface area, and tunable pore structures, have emerged as promising host materials for advanced functional applications. In this study, two organic dyes, fluorescent bleacher (CBS) and coumarin (C6), exhibiting blue and green fluorescence, respectively, were co-encapsulated into europium (Eu)-based MOFs, where Eu served as the central metal to introduce red emission. The ligand employed was 9-butylcarbazole-2-carboxylhydrazide thiophene (Cz).

MRI-Based Assessment of Margin Status Determinants, Preoperative Predictors, and Prognostic Outcomes in Breast-Conserving Surgery Following Neoadjuvant Therapy.

Background And Purpose: This MRI-based study investigates factors influencing and predictors of margin status in neoadjuvant therapy followed by patients with breast-conserving surgery (NAT-BCS) and evaluates associated locoregional/distant control outcomes.

Materials And Methods: We conducted a retrospective analysis of 117 consecutive patients with breast cancer (BC) who underwent NAT-BCS between June 2014 and June 2024. All patients received magnetic resonance imaging (MRI) examinations both before and after NAT.

Healthy sleep patterns and risk of incident cardiovascular disease, liver-related events, and all-cause mortality among individuals with metabolic dysfunction--associated steatotic liver disease: A large prospective cohort study.

Objectives: This study aimed to investigate the association between overall healthy sleep patterns and cardiovascular disease, liver-related events, and all-cause mortality among individuals with metabolic dysfunction--associated steatotic liver disease.

Methods: This cohort study included 123,228 participants with metabolic dysfunction--associated steatotic liver disease from the UK Biobank, who had detailed sleep assessment at baseline. The healthy sleep pattern was constructed by a composite healthy sleep score that incorporated different sleep behaviors.

The effects of landscape on visual preference and fatigue recovery among university students: Differences in gender, grade level and major.

Exposure to natural landscapes has been shown to affect both physiological and psychological well-being, with the extent of these effects varying across different landscape types. However, the underlying mechanisms remain poorly understood. The association among stress reduction, environments characteristics and individual differences requires further investigation, particularly considering the complexity of landscape attributes and the variability of personal responses.

Contribution of Protected Area Networks to Achieving Global Biodiversity Framework Targets and Sustainable Development Goals: Evidence From Coastal Shandong, China.

The protected area network (PAN) is a vital strategy for enhancing ecological integrity and connectivity. However, its contributions to the Kunming-Montreal Global Biodiversity Framework (GBF) 2030 Targets and Sustainable Development Goals (SDGs) remain understudied due to limited indicator assessments and regional case comparisons. This study applied circuit theory to construct and optimize a PAN in China's coastal region.

Evaluating the utility of enhanced T1 mapping MR imaging in assessing depth of myometrial invasion and detecting DNA mismatch repair status in endometrial cancer: a pilot study.

Objectives: To evaluate the value of enhanced T1 mapping MR imaging in assessing the depth of myometrial invasion (MI) and in detecting DNA mismatch repair (MMR) status in endometrial cancer (EC) as a non-invasive imaging biomarker.

Methods: This prospective study enrolled 46 patients with pathologically confirmed EC who underwent pelvic MRI and surgery within two weeks. Each patient underwent multiparametric MRI including T2-weighted imaging (T2WI), diffusion-weighted imaging (DWI), dynamic contrast-enhanced imaging (DCE), native T1 mapping and enhanced T1 mapping.

Rapid screening and annotation of the carbonyl submetabolome via bromine isotope-based derivatization sensitization assisted by structure-guided molecular network strategy.

Background: Carbonyl compounds (CCs) are extensively present in biological and food samples with broad concentration range and high reactivity. Despite advances in chemical isotope labelling assistant liquid chromatography-mass spectrometry (CIL-LC-MS) to address poor ionization efficiency and weak chromatographic retention, challenges remain in defining potential CCs by report ion and further accurately annotating labelled products due to the lack of experimental spectra. Improved strategies are needed to enhance the annotation of CCs.

Corticospinal Excitability in Bilateral M1 Hand Areas: Association with Neuropathic Pain After Spinal Cord Injury.

Purpose: Neuropathic pain (NP) is a major and debilitating complication of spinal cord injury (SCI), frequently occurring bilaterally below the injury level. Repetitive transcranial magnetic stimulation (rTMS) targeting the primary motor cortex (M1) is suggested as a treatment for NP following SCI (SCI-NP). However, the specific changes in corticospinal excitability (CSE) within the bilateral M1 remain unclear, hindering the development of optimized rTMS parameters for SCI-NP.

The biomechanical changes of facet joint violation after transforaminal lumbar interbody fusion combined with decompression surgery: a finite element study.

Introduction: Facet joint violation (FJV) is a common complication of intervertebral fusion surgery, altering the load-bearing capability of the facet joints and ultimately contributing to segmental instability. Furthermore, adjacent segment degeneration is one of the potential long-term complications following lumbar spinal intervertebral fusion. For patients with a history of lumbar intervertebral fusion who developed symptomatic spinal stenosis at adjacent segments, adjacent segment decompression surgery is a clinically viable option.

Global trends in machine learning applications for single-cell transcriptomics research.

Background: Single-cell RNA sequencing (scRNA-seq) has revolutionized cellular heterogeneity analysis by decoding gene expression profiles at individual cell level, while machine learning (ML) has emerged as core computational tool for clustering analysis, dimensionality reduction modeling and developmental trajectory inference in single-cell transcriptomics(SCT). Although 3,307 papers have been published in past two decades, there remains lack of bibliometric review comprehensively addressing methodological evolution, technical challenges and clinical translation pathways. This study aims to fill research gap through bibliometric and visual analysis, revealing technological evolution trends and future development directions.

Effects of injecting micro-fragmented adipose tissue (MFAT) in the tendon-bone junction region during anterior cruciate ligament reconstruction on postoperative tendon-bone healing: a protocol for a randomised controlled trial in China.

Introduction: Anterior cruciate ligament (ACL) injuries are among the most common sports-related injuries, often requiring surgical reconstruction to restore joint stability and function. However, inflammation and graft-to-bone healing remain critical challenges in postoperative recovery. Micro-fragmented adipose tissue (MFAT), rich in mesenchymal stem cells and anti-inflammatory factors, has shown potential in promoting tissue repair and reducing inflammation.