Design and Evolution of an Artificial Aldolase with an Abiological Catalytic Cofactor in Apo-Myoglobin.

Current research on artificial aldolases predominantly centers on aldehyde substrates with relatively limited exploration of ketone substrates. Here, we report the creation of a novel artificial aldolase based on apo-myoglobin by embedding an organocatalytic secondary amine cofactor in the protein's distal pocket. The designer enzyme exhibits good to excellent enantioselectivities (up to 97.

Novel ternary deep eutectic solvent coupled with in-situ-ultrasound synergistic extraction of flavonoids from Epimedium wushanense: machine learning, mechanistic investigation, and antioxidant activity.

This study innovatively developed a novel ternary deep eutectic solvent coupled with in-situ-ultrasound synergistic extraction (TDES-ISUSE)method for efficient extraction of total flavonoids (TF) fromEpimedium wushanense. Among 20 prepared DESs, the ternary system composed of choline bitartrate, urea, and glycerol (Chb:Ure:Gly) exhibited the highest extraction efficiency. Response surface methodology-artificial neural network-genetic algorithm (RSM-ANN-GA) optimization identified the optimal extraction parameters: water content of 32 %, vortex time of 10 min, liquid/solid ratio of 34:1 mL/g, ultrasound time of 30 min, and ultrasound power of 270 W.

Efflux pumps positively contribute to rifampin resistance in rpoB mutant Mycobacterium tuberculosis.

Background: While several recent studies have documented the importance of efflux pumps as mediators of rifampin (RIF) resistance, it remains uncertain which efflux pumps play major roles in rifampin-resistant Mycobacterium tuberculosis strains harboring rpoB gene mutations.

Methods: In this study, minimum inhibitory concentration (MIC) values for RIF were calculated and the expression of 13 efflux pump genes was evaluated across 35 clinical rifampicin-resistant M. tuberculosis isolates carrying the rpoB mutation before and after efflux pump inhibitor treatment.

Evaluating Temporomandibular Disorder Severity: A Four-Dimensional Model.

Background: This study developed and tested a multi-dimensional model for evaluating the severity of temporomandibular disorders (TMDs) based on the five core symptoms (5Ts) of the Diagnostic Criteria for TMDs (DC/TMD).

Methods: Study participants were sourced from a university hospital. The 5Ts screener was expanded to include the dimensions of duration, frequency, intensity, and interference with daily activities (5Ts-4D).

Filament elongation and pollen development regulated by auxin is crucial for male fertility in Arabidopsis.

The flavin monooxygenase encoded by YUCCA (YUC) is the rate-limiting enzyme in auxin biosynthesis, and its spatiotemporal expression regulates local auxin biosynthesis, resulting in a dose-dependent regulation of flower development. Previous research reported that decreased fertility in yuc2yuc6 double mutant was caused by abnormal development of pollen. In this study, we found that yuc2yuc6 showed different fertility between early (growth stage 6.

Coupling mechanisms of community assembly and pollutant removal in algal-bacterial granular sludge systems.

This study comparatively assessed algal-bacterial granular sludge (ABGS) decontamination performance and microbial community mechanisms under two cultivation modes: flocculated sludge transformed into ABGS (AS_ABGS) and aerobic granular sludge transformed into ABGS (AGS_ABGS). The results indicated that, AS_ABGS achieved superior pollutant removal (COD: 92.2 %, TN: 82.

A sonoelectric niche for noninvasive intervertebral disc regeneration via targeted cell cycle modulation.

Cell cycle regulation is pivotal for tissue regeneration yet remains challenging in degenerative microenvironments. We engineered a sonosensitive conductive hydrogel incorporating polypyrrole-encapsulated porphyrin derivatives {[Tetrakis (4-carboxyphenyl) porphyrin (TCPP)]@PPy} to regulate cell cycle dynamics. Upon ultrasound irradiation, TCPP@PPy generates free electrons, establishing a controlled microcurrent within degenerative tissues.

Physiological and psychological symptom management based on electronic patient-reported outcomes: the TD-WELLBEING randomized clinical trial.

Background: One-third of all lung cancer cases globally are reported in China. This study evaluated the symptom management efficacy of an electronic patient-reported outcomes (ePRO)-based intervention for postoperative symptoms like pain and psychological distress after lung cancer surgery.

Methods: We included lung cancer surgery patients (April 2022-October 2023; age, 18-75 years) with ECOG scores of 0-2 and expected survival of >6 months and randomized them into control and intervention groups.

Effects of Er: YAG laser and acid etching on bond strength of clear aligner attachments to fluorotic enamel.

Background: There is a lack of research on the bonding ability of attachments to fluorosis enamel. This study evaluates Er: YAG laser-assisted acid etching as a potential optimization protocol.

Methods: Twenty healthy teeth and ninety fluorotic teeth (Thylstrup-Fejerskov Index = 4) were divided into a control group (healthy enamel + acid etching) and a fluorotic group (acid etching 30/60/90 seconds vs.

Prognosis and Factors Influencing Temporomandibular Joint Degenerative Disease in Adolescents: A Retrospective Study.

Background: Few studies have addressed the prognosis of temporomandibular joint degenerative joint disease (TMJ-DJD) in adolescents.

Objective: To retrospectively analyse clinical and imaging data of adolescent patients (10-19 years) with TMJ-DJD who had multiple follow-up visits, focusing on condylar bone outcomes and influencing factors.

Methods: Clinical records and CBCT scans of adolescent TMJ-DJD patients were reviewed.

Constructing Continuously-Distributed and Crystalline-NaF-Rich SEI on Hard Carbon Anode Through Binder Chemistry for High-Performance Sodium-Ion Batteries.

Constructing the continuously-distributed and crystalline-NaF-rich solid electrolyte interface (CC-NaF-SEI) is expected to greatly promote the sodium storage performance of hard carbon (HC) anodes. However, such an impressive concept remains extremely intractable to achieve and lacks an efficiently cost-less strategy. Herein, the application of the commercially available LA133 binder is pioneered to engineer such a CC-NaF-SEI.

Effects of co-metabolic carbon sources on the formation of Algal-Bacterial Granular Sludge and pollutant degradation under quinoline stress: sludge characteristics, degradation pathways, and molecular mechanisms.

Algal-bacterial granular sludge (ABGS) technology exhibits efficient pollutant metabolic capacity through synergistic algal-bacterial interactions, demonstrating substantial potential for sustainable industrial wastewater treatment. However, maintaining stable and efficient operation under quinoline stress remains a critical challenge. This study systematically examined the effects of co-metabolic carbon source type (starch or phenol) on sludge characteristics, pollutant removal performance, quinoline degradation pathways, and the microbial community ecology in ABGS under quinoline stress.

An artificial intelligence modeling framework based on microbial community structure prediction enhances the pollutant removal efficiency of the algae-bacteria granular sludge system.

Algae-bacteria granular sludge (ABGS) technology is a new energy-saving and low-carbon water treatment technology based on the algae-bacteria symbiotic system. However, due to its complex internal microbial system, the regulation mechanism of ABGS is unclear. To address this issue, the present study constructed a two-stage optimal control model for the ABGS system, which includes prediction of microbial community structure and planning of pollutant removal efficiency.

Addressing osteoblast senescence: Molecular pathways and the frontier of anti-ageing treatments.

Background: Osteoblast senescence is a central driverof age-related osteoporosis. Accumulating evidence shows that counteractingthis senescence can substantially mitigate bone loss. In this review, we summarize the hallmarks of osteoblast senescence, the signaling pathways involved, and therapeutic strategies that target osteoblast senescence tocombat age-related osteoporosis.

Limited benefit of adjuvant chemotherapy for Asian patients with stage IB lung adenocarcinoma: implications for clinical practice.

Backgroud: The benefit of adjuvant chemotherapy for patients with stage IB lung adenocarcinoma remains unclear. We compared adjuvant chemotherapy outcomes in Asian and non-Asian stage IB lung adenocarcinoma patients using multi-source clinical data.

Methods: Patients with stage IB lung adenocarcinoma from the 2004–2015 Surveillance, Epidemiology, and End Results (SEER) database and the 2020–2021 thoracic surgery department of three Chinese medical centers were included.

DDX1 methylation mediated MATR3 splicing regulates intervertebral disc degeneration by initiating chromatin reprogramming.

Low back pain (LBP), primarily driven by intervertebral disc degeneration (IVDD), has become a core challenge in public health. DDX1, an RNA-binding protein, plays key roles in RNA metabolism but its function in IVDD remains unclear. We identify DDX1 as a substrate of methyltransferase EZH2, which methylates DDX1 at lysine 234 (K234), promoting IVDD in vitro and in vivo.

Expert Consensus on Subxiphoid and Subcostal Arch Thoracoscopic Resection for the Treatment of Thymoma.

Surgery is the primary treatment for thymoma. Although subxiphoid and subcostal arch thoracoscopic thymoma surgery is widely used, there is currently a lack of consensus regarding its use, nor have standards been established. Based on the surgical experience of many domestic thoracic surgery centers, the Department of Thoracic Surgery of Tangdu Hospital of Air Force Medical University has formulated this expert consensus regarding key clinical issues related to thoracoscopic thymoma surgery, including preoperative evaluation, surgical indications, preoperative preparation, surgical details, perioperative management, postoperative treatment, and follow-up.

Machine Learning Models Based on CT Enterography for Differentiating Between Ulcerative Colitis and Colonic Crohn's Disease Using Intestinal Wall, Mesenteric Fat, and Visceral Fat Features.

Purpose: This study aimed to develop radiomic-based machine learning models using computed tomography enterography (CTE) features derived from the intestinal wall, mesenteric fat, and visceral fat to differentiate between ulcerative colitis (UC) and colonic Crohn's disease (CD).

Methods: Clinical and imaging data from 116 patients with inflammatory bowel disease (IBD) (68 with UC and 48 with colonic CD) were retrospectively collected. Radiomic features were extracted from venous-phase CTE images.

YAP Deficiency in Gli1 Osteogenic Progenitors Leads to Condylar Hypoplasia via β-Catenin Signaling.

Objectives: The pathogenesis of mandibular condylar hypoplasia has not been fully understood. Our previous studies provided evidence that Gli1 osteogenic progenitors contribute to condylar development. This study aimed to investigate the role of YAP in Gli1 cells during condylar development.

Theta oscillation synchronize VTA and mPFC during ethanol-induced conditioned place preference and stress-evoked anxiety.

Addiction and anxiety are two prevalent psychiatric conditions characterized by complex neurobiological mechanisms. It is supported by theta-range (4-12 Hz) neural oscillations that coordinate distant brain regions, such as ventral tegmental area (VTA) and medial prefrontal cortex (mPFC). However, the systematic investigation comparing the analogous and distinct mechanisms of theta oscillations in the mPFC and VTA across these two psychiatric disorders is still needed.

A study on the prediction of targeted therapy efficacy in advanced lung adenocarcinoma patients with EGFR mutations using CT-based delta-radiomics model.

Objective: This study aimed to evaluate the predictive performance of integrated clinical and CT-based radiomic models for assessing targeted therapy efficacy in advanced lung adenocarcinoma patients with EGFR (epidermal growth factor receptor) mutations.

Materials And Methods: This retrospective study included 106 EGFR-mutated advanced lung adenocarcinoma patients treated with targeted therapies at the Second Hospital of Jilin University (2020-2023). Patients were classified as responders (PR) or non-responders (SD/PD) based on RECIST (Response Evaluation Criteria in Solid Tumors) 1.

VTA-ACC dopaminergic circuit mediates trait anxiety-related observational learning of social avoidance in male mice.

Social animals encounter both environmental and social stress, yet the mechanisms by which individuals with different levels of trait anxiety cope with these stressors, as well as the neurobiological links between trait anxiety and social cognition, remain incompletely understood. Here, male mice are classified into high-trait anxiety (HTA) and low-trait anxiety (LTA) groups based on their anxiety responses to elevated platform exposure in the open field test. Under observational learning-based vicarious social defeat stress (VSDS), HTA mice exhibit less social avoidance behavior toward CD1 aggressors than LTA mice.

Early versus late pancreatic stent placement for preventing post-ERCP pancreatitis: protocol of a multicenter randomized clinical trial.

Background: Pancreatic stenting has been shown to effectively lower the occurrence of post-ERCP pancreatitis (PEP) and reduce its severity. To date, no evidence exists to determine the optimal timing for pancreatic stent placement in prospective trials. Our aim is to compare early versus late pancreatic stent placement in preventing PEP among patients with naive papilla.

Response of extracellular polymeric substances in algal-bacterial granular sludge under salinity stress: Secretion behavior, structural properties, and protective roles.

The algal-bacterial granular sludge (ABGS) technology exhibits the advantages of low energy consumption and high shock load resistance. While ABGS regulates extracellular polymeric substances (EPS) secretion to cope with adverse conditions, the role of EPS in resisting salinity stress remains uncertain. This study investigated the response ofEPS in ABGS to salinity stress (0-5 %) by examining secretion behavior, structural properties, and protective roles.