MLKL PARylation in the endothelial niche triggers angiocrine necroptosis to evade cancer immunosurveillance and chemotherapy.

Chemoresistance is the leading cause of cancer-related death. How chemotherapy subjugates the cellular crosstalk in the tumour microenvironment to cause chemoresistance remains to be defined. Here we find chemotherapy enables immunosuppressive SDF1 endothelial niche to evade immunosurveillance in ovarian and breast cancers.

Metabolomics navigates natural variation in pathogen-induced secondary metabolism across soybean cultivar populations.

e-induced root rot poses a major threat to soybean production. While the molecular mechanisms underlying soybean- interactions have been extensively studied, their biochemical basis remains largely unexplored. Previous research has identified key metabolic modules involved in pathogen defense, but structural diversity has largely been constrained by studies on single soybean accessions.

m6A Modified-CYP1B1 Promotes HCC Cell Proliferation by Inhibiting Ferroptosis.

Introduction: CYP1B1, a crucial drug-metabolizing enzyme, metabolizes both endogenous compounds and clinical drugs. The present study investigated the effects of CYP1B1 on the proliferation, migration, apoptosis, and ferroptosis of HCC cells. It further elucidated the regulatory role of m⁶A modification, particularly via the methyltransferase METTL14-in regulating CYP1B1 mRNA stability and translation efficiency.

Actinobacteria mediate anaerobic methane oxidation in iron-rich environment.

Recent research has identified the Actinobacterium Candidatus Mycobacterium methanotrophicum, which oxidizes methane aerobically in extremely acidic environments. In a previous study, we observed significant enrichment of Actinobacteria in an anaerobic iron-rich methane-oxidation reactor. Therefore, in this study, we assessed the anaerobic methane utilization potential of Actinobacteria using microcosm experiments, isotope labeling, and DNA stable isotope probing.

Transmembrane nanogold energy transfer ruler enables synchronized projecting of receptor dimerization-phosphorylation signaling.

Nanoscale dimerization geometries dictate transmembrane receptor phosphorylation dynamics, yet current methods fail to directly correlate spatial receptor organization with real-time phosphorylation states . Receptor tyrosine kinase (RTK) activation, essential for cellular signaling, is dynamically controlled by dimerization at the nanoscale, but existing tools cannot simultaneously resolve spatial dimer configurations and phosphorylation activity. Here, we developed a transmembrane nanogold surface energy transfer ruler (T-Nanoruler) to enable tracking of RTK activation.

-Palmitoleic Acid Regulates Lipid Metabolism via Diacylglycerol Metabolic Shunting.

Obesity and related metabolic disorders are closely linked to dysregulated lipid metabolism, where the metabolic balance of diacylglycerol (DAG) played a pivotal role. Although -palmitoleic acid (POA) exhibits anti-obesity effects, its efficacy varies across dietary conditions, and its molecular mechanisms remains unclear. In this study, we investigated the dose-dependent regulatory effects of POA on DAG metabolic shunting in db/db mice, employing lipidomics, pathway analysis, and gene/protein expression assays.

The antibody-drug conjugate SHR-A1904 for targeting CLDN18.2 in advanced gastric or gastroesophageal junction cancer: a phase 1 trial.

Claudin-18 isoform 2 (CLDN18.2), a tight junction protein expressed in non-malignant gastric epithelium and exposed on tumor cell surface during malignant transformation, is a promising therapeutic target for gastric and gastroesophageal junction (G/GEJ) cancers. SHR-A1904 is an antibody-drug conjugate comprising CLDN18.

Evaluating the tumor response assessment of modified-RECIST 1.1 in advance non-small cell lung cancer: a analysis of 1,147 patients from the EAST-LC trial.

Background: Response Evaluation Criteria in Solid Tumors version 1.1 (RECIST 1.1) provides conventional response evaluation in solid tumors.

Advances in Targeted Therapy for Brain Metastases in Human Epidermal Growth Factor Receptor 2 (HER2)-Positive Breast Cancer: A Focus on ADCs and TKIs.

Aim: Brain metastasis remains a significant therapeutic challenge in HER2-positive breast cancer, contributing to poor prognosis and limited treatment options. This review aims to summarize recent advancements in targeted therapies for brain metastasis in HER2-positive breast cancer, with a focus on the efficacy, mechanisms, and clinical implications of antibody-drug conjugates (ADCs) and tyrosine kinase inhibitors (TKIs).

Methods: We conducted a comprehensive review of clinical trials, real-world studies, and preclinical research.

Context-dependent anaerobic oxidation of methane: Insight for methane emission mitigation.

Anaerobic oxidation of methane (AOM) exhibits context-dependent metabolic versatility, governed by electron acceptor heterogeneity and anthropogenic perturbations. This study investigates the AOM potential by simulating three environments, high-dissolved organic carbon (DOC), high-nitrate with moderate sulfate, and sulfate-enhanced conditions, to investigate AOM potential under controlled perturbations. Substrate conversion dynamics were observed by monitoring the variation of methane, sulfate, nitrate, iron, etc.

RNA-binding proteins DND1 and NANOS3 cooperatively suppress the entry of germ cell lineage.

Specification of primordial germ cells (PGCs) establishes germline development during early embryogenesis, yet the underlying mechanisms in humans remain largely unknown. Here, we reveal the functional roles of germline-specific RNA-binding protein (RBP) DND1 in human PGC (hPGC) specification. We discovered that DND1 forms a complex with another RBP, NANOS3, to restrict hPGC specification.

Advanced fluidized bed-ultrafiltration-reverse osmosis process for near-complete fluorine recovery from photovoltaic fluoride-laden wastewater: system parameters and mechanisms.

Industrial fluoride-laden wastewater has emerged as a critical environmental challenge due to the rapid growth of photovoltaics and battery industries. This study proposes a novel, integrated and expandable fluidized bed-ultrafiltration-reverse osmosis (FBUR) process. The fluidized bed component targets F crystallization, while ultrafiltration (UF) and reverse osmosis (RO) membrane units concentrates fluidized bed effluent.

Electrochemical-Genetic Programming of Protein-Based Magnetic Soft Robots for Active Drug Delivery.

Magnetic soft robots have the potential to revolutionize the field of drug delivery owing to their capability to execute tasks in hard-to-reach regions of living organisms. Advancing their functionality to perform active drug delivery and related tasks necessitates the innovation of smart substrate materials that satisfy both mechanical and biocompatibility requirements while offering stimuli-responsive properties. Optimization of the interaction between the substrate and magnetic components is also critical as it ensures robust actuation of the robot in complex physiological environments.

Safety, pharmacokinetics and efficacy of HA121-28 in patients with advanced solid tumors and RET fusion-positive non-small-cell lung cancer: a multicenter, open-label, single-arm phase 1/2 trial.

HA121-28, a promising multikinase inhibitor, mainly targets rearranged during transfection (RET) fusions and selectively targets vascular endothelial growth factor receptor-2, endothelial growth factor receptor, and fibroblast growth factor receptor 1-3. The safety, pharmacokinetics, and efficacy of HA121-28 were assessed in advanced solid tumors (phase 1, ClinicalTrials.gov NCT03994484) and advanced RET fusion-positive non-small-cell lung cancer (RET-TKI naive NSCLC, phase 2, ClinicalTrials.

Urinary metabolomics analysis of patients with renal tubular dysfunction after PCI surgery.

Purpose: Differences in metabolic profiles were used to search for urinary metabolic markers in patients with tubular dysfunction after percutaneous coronary intervention (PCI).

Methods: In this study, 50 patients with renal tubule dysfunction 6 h after PCI were collected and urine samples before PCI were used as control group and 6 h after PCI were used as observation group. The urine samples were processed by high-speed centrifugation, filtered by microporous membrane, and the superclear was obtained for LC-MS detection and analysis, and their metabolic profiles and related data were obtained.

Preparation of composite film based on corn starch and soybean fiber composites blended in 1-ethyl-3-methylimidazolium chloride.

Background: To overcome the deficiency of pure starch in film-forming performance, ionic liquids (ILs) have been introduced when preparing a homogeneous blend of starch and fiber. It is essential to select the appropriate ratio of starch to fiber and evaluate the film-forming property of the resulted composites. In the present study, 1-ethyl-3-methylimidazolium chloride ([EMIM]Cl) was used to prepare composites of soybean fiber (SF) and corn starch (CS) at various ratios.

Response of soil biochemical properties and ecosystem function to microplastics pollution.

Microplastics (MPs)-induced changes in soil nutrient cycling and microbial activity may pose a potential risk to soil ecosystem. Although some studies have explored these topics, there is still a large space for exploration and a relative lack of research on the mechanism by which soil health and its functions are affected by these changes. Thus, this study investigated the effects of polyethylene (PE) MPs with two particle sizes (13 μm and 130 μm) at five concentrations (0%, 1%, 3%, 6% and 10%, w/w) on soil biochemical properties and ecosystem function.

Molecular prevalence, subtype distribution, and zoonotic potential of sp. in wild rodents and shrews inhabiting Zhejiang province of China.

Introduction: Globally, rodents and shrew populations constitute crucial elements of diverse environments and animal communities. It is imperative to study their population dynamics to mitigate any potential negative impact on humans, as they can be involved in the transmission of critical zoonotic agents, such as . Therefore, this study aimed to identify the prevalence and genetic composition of in wild rodents and shrews residing in the Zhejiang provinces of China.

Decoding the biochemical dialogue: metabolomic insights into soybean defense strategies against diverse pathogens.

Soybean, a crucial global leguminous crop, confronts persistent threats from diverse pathogens, exerting a profound impact on global yields. While genetic dimensions of soybean-pathogen interactions have garnered attention, the intricate biochemical responses remain poorly elucidated. In this study, we applied targeted and untargeted liquid chromatography coupled to mass spectrometry (LC-MS) metabolite profiling to dissect the complex interplay between soybeans and five distinct pathogens.

Bioinspired Genetic and Chemical Engineering of Protein Hydrogels for Programable Multi-Responsive Actuation.

Protein hydrogels with tailored stimuli-responsive features and tunable stiffness have garnered considerable attention due to the growing demand for biomedical soft robotics. However, integrating multiple responsive features toward intelligent yet biocompatible actuators remains challenging. Here, a facile approach that synergistically combines genetic and chemical engineering for the design of protein hydrogel actuators with programmable complex spatial deformation is reported.

Comparative proteomics analysis of revealed a variety of regulatory systems on conidiospore formation.

is a typical parasitic medicinal fungus of the family Shiraiaceae. The fruiting bodies of cannot be cultivated artificially, and active substances can be effectively produced via fermentation. The mechanism of conidia production is a research hotspot in the industrial utilization and growth development of .

Factors associated with postoperative efficacy evaluation in patients with morbid obesity.

The global obesity problem is becoming increasingly serious, with eight of the top ten causes of death in Taiwan in 2020 being related to obesity. Morbid obesity poses a significant threat to one's health and well-being. In recent years, bariatric surgery has emerged as a more effective treatment option for patients with morbid obesity.