Next-generation sequencing identifies the mutational signature of double primary and metastatic malignancies: A case report.

We present the case of a 59-year-old Chinese man diagnosed with stage III clear cell renal cell carcinoma who developed 2 suspicious lung lesions 5 years after follow-up. Pathological evaluation revealed 2 distinct types of cancer: lung adenocarcinoma in situ and clear cell renal carcinoma with lung metastasis. Lung tissue samples were sequenced using a panel of 1267 cancer-related genes.

Digital camouflage encompassing optical hyperspectra and thermal infrared-terahertz-microwave tri-bands.

Modern reconnaissance technologies, including hyperspectral and multispectral intensity imaging across optical, thermal infrared, terahertz, and microwave bands, can detect the shape, material composition, and temperature of targets. Consequently, developing a camouflage technique that seamlessly integrates both spatial and spectral dimensions across all key atmospheric windows to outsmart advanced surveillance has yet to be effectively developed and remains a significant challenge. In this study, we propose a digital camouflage strategy that covers the optical (0.

Investigation on the Inhibitory Mechanism of Chai Hu Hua Ji Tang on Liver Cancer Based on a Network Pharmacology Analysis.

Hepatocellular carcinoma (HCC) poses a serious threat to human health due to its high incidence and mortality rates. In recent years, the application of traditional Chinese medicine (TCM) in the comprehensive treatment of liver cancer has gained increasing attention. Clinical practices have demonstrated the mighty efficacy of Chai Hu Hua Ji Tang (CHHJT) in liver cancer treatment, yet its underlying mechanism remains unexplored.

Identification and Experimental Validation of Oxidative Stress-Related Biomarkers in Ulcerative Colitis Using Machine Learning.

Purpose: Ulcerative colitis (UC) remains challenging to diagnose and treat due to a lack of reliable biomarkers. This study investigates oxidative stress-related targets in UC using bioinformatics and experimental validation.

Methods: We analyzed four GEO datasets and oxidative-stress genes from MSigDB, applying differential analysis, LASSO regression (for feature selection), and random forest (for robust biomarker identification).

Light-Controlled Oscillation of Microplates Leveraging Contact Adhesion.

Miniaturized mechanical oscillation is mostly realized in microelectromechanical systems (MEMS). Its routine implementation involves carefully configuring a driving force, such as electrostatic force, while concurrently mitigating contact adhesion that tends to anchor moving parts. Though often undesirable, contact adhesion plays a significant role at micronano scales and may be harnessed as a driving force for miniaturized oscillators.

Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting.

Chimeric antigen receptor (CAR) T cell therapy has shown promise in treating hematologic malignancies, but it still faces challenges, including high costs, a time-consuming manufacturing process, and the necessity of lymphodepletion. Here, we generate circular RNAs (circRNAs) encoding CAR proteins, referred to as circRNA, which mediates remarkable tumor killing in human primary T cells. We demonstrate that circRNA, delivered with immunocyte-tropic lipid nanoparticles (LNPs), can form in vivo panCAR cells (CAR-T, CAR-natural killer [NK], and CAR-macrophage), significantly inhibit tumor growth, and reshape the tumor microenvironment in mice.

The effect of Akkermansia in patients with pregnancy induced hypertension.

Backgrounds: Intestinal dysbiosis in the second trimester is associated with pregnancy-induced hypertension (PIH) in the first trimester. However, the consequences and underlying mechanisms remain unclear.

Methods: In a follow-up cohort study, a nested case-control design was employed.

Oligometastatic Renal Ewing Sarcoma With Fusion: A Case Report And Literature Review.

Ewing sarcoma (ES) is an undifferentiated round cell sarcoma arising in skeletal and extraskeletal locations usually diagnosed in the second decade of life. It is typically characterized by a monomorphic round blue cell morphology, positive membranous CD99 immunohistochemical staining, and gene fusion with a partner gene from the ETS family. Less frequently, other genes from the FET family of genes substitute for .

Exploring the masking of berberine bitterness by natural polysaccharides based on a "net-hook" dual-effect mechanism.

Bitterness masking is a crucial technical hurdle in the development and application of oral liquid medicines. Small-molecule excipients, such as cyclodextrin, often provide insufficient bitterness masking, thus prompting the exploration of large-molecule excipients, especially polysaccharides. In this study, the bitterness-masking effects of 10 polysaccharides were assessed using berberine as a model.

Versatility of LNPs across different administration routes for targeted RNA delivery.

The advent of lipid nanoparticle (LNP) technology has marked a significant breakthrough in the field of drug delivery, offering unprecedented opportunities in gene therapy, vaccine delivery, and personalized medicine. The use and effectiveness of LNPs greatly depend on their optimization, often tailored to specific routes of administration. Different routes can significantly influence organ distribution, expression kinetics and therapeutic outcomes of LNPs, with the choice of the route dependent on LNP properties, target sites, and therapeutic indications.

The association between circulating palmitic acid levels and risk of premature coronary artery disease in Chinese patients: a case-control study.

Background: Palmitic acid (PA) is a risk factor for atherosclerosis but its significance in premature coronary artery disease (PCAD) remains poorly understood. Herein, we investigated the impact of circulating PA levels on the risk of PCAD occurrence in Chinese patients.

Methods: In this case control study, we included patients diagnosed with PCAD and age-matched non-CAD controls between June 2022 and December 2023.

Glucosamine alleviates liver fibrosis through modulation of sinusoidal blood flow and hepatic immune responses.

Liver fibrosis, a key precursor to cirrhosis, arises from chronic liver injury and is marked by excessive deposition of extracellular matrix proteins, primarily collagen. This fibrotic remodeling is driven by activation of hepatic stellate cells (HSCs), which are influenced by immune responses from diverse immune cell types, particularly from macrophages. Despite considerable advances in understanding fibrosis pathophysiology, no effective antifibrotic therapies are currently available.

Honokiol in the treatment of triple-negative breast cancer: a network pharmacology approach and experimental validation.

Triple-negative breast cancer (TNBC) is a rare and highly metastatic form of cancer. Honokiol (HNK), a biphenolic compound, has been utilized in TNBC treatment, though its specific targets remain unclear. This study aimed to elucidate the effects of HNK on TNBC by combining network pharmacology predictions and experimental validation to uncover its mechanisms.

Prominent supramolecular systems for cancer Therapy: From structural design to tailored applications.

Supramolecular materials represent a powerful class of platforms in cancer diagnosis and therapy, owing to their dynamic architectures, stimuli responsiveness, and high biocompatibility. This review focused on three representative categories-Pillarene-based systems, virus-mimetic nanoparticles (VMNs), and metal-organic frameworks (MOFs)-each offering unique structural and functional properties. Pillarene-based assemblies enable precise host-guest interactions, by being classified into amphiphilic, ionic, and chiral varieties, the robust drug loading and controlled release capabilities of the Pillarene family were emphasized.

Clinical features of non-compaction cardiomyopathy across age groups: a retrospective study of 415 patients.

Background: Non-compaction cardiomyopathy (NCM) is a rare inherited cardiac disorder associated with adverse cardiovascular outcomes, including heart failure, arrhythmias and sudden cardiac death. Currently, the clinical manifestations of NCM lack comprehensive characterisation across different age groups in large-scale studies. This investigation aims to systematically analyse the clinical characteristics of patients with NCM across various age demographics.

Commercially Available Blue Diode Laser Engraver Operating at 455 nm as an Affordable LD-REIMS Ionization Source.

Lasers are commonly used for mass spectrometric applications to perform laser ablation-desorption and ionization; however, the use of visible light is not very common. Here, we report a commercially available visible light laser engraver operating at 455 nm as an ionization source, generating rich spectral profiles featuring predominantly lipid species, such as fatty acids and glycerophospholipids. Laser settings such as the speed of movement over the sample and laser power were tested, resulting in an optimum laser speed of 300 mm/min and a laser power of 30-50% for the analysis of fresh salmon tissue samples.

Free-space high-Q nanophotonics.

High-Q nanophotonic devices hold great importance in both fundamental research and engineering applications. Their ability to provide high spectral resolution and enhanced light-matter interactions makes them promising in various fields such as sensing, filters, lasing, nonlinear optics, photodetection, coherent thermal emission, and laser stealth. While Q-factors as large as 10 have been achieved experimentally in on-chip microresonators, these modes are excited through near-field coupling of optical fibers.

WWP1-SHARP1-C/EBPβ positive feedback loop modulates development of metabolic dysfunction-associated steatotic liver disease.

Background & Aims: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a significant global health threat. The molecular mechanisms underlying regulation of MASLD remain largely unknown. This study aimed to investigate the role of the WW domain-containing ubiquitin E3 ligase 1 (WWP1)-enhancer-of-split and hairy-related protein 1 (SHARP1)-C/EBPβ signal loop in MASLD.

Specific selection on XEG1 and XLP1 genes correlates with host range and adaptability in Phytophthora.

In diverse Phytophthora-plant pathosystems, Phytophthora secretes XLP1 (PsXEG1-Like Protein), a non-enzymatic paralog that functions as a decoy to protect XEG1 (Xyloglucan-specific Endoglucanase) from host inhibitors. Here, we show that the genus-specific selection pressures on the XEG1/XLP1 gene pair are crucial for host adaptation and are closely linked to Phytophthora host range. Our findings reveal that the XEG1/XLP1 gene pair originated within Phytophthora and subsequently evolved into genus-specific genes, undergoing functional divergence driven by preferential selection.

Dimethylamino-based synthetic lipidoid nanoparticles for selective mRNA delivery to splenic antigen-presenting cells.

Targeted systemic mRNA delivery to extrahepatic tissues remains a formidable challenge, especially in the absence of targeting ligands on lipid nanoparticles. In this study, we introduce a series of dimethylamino-based ionizable lipidoids (DMA-Lipidoids) engineered for selective mRNA delivery to the spleen. Using a combinatorial approach, we synthesized 48 chemically distinct lipidoids by pairing four DMA-containing amine heads with 12 newly designed hyperbranched tails.

Carrageenan-xanthan gum composite gel achieves the elimination of bitterness and easy swallowability of Coptis chinensis based on electrostatic induction and interpenetrating network interaction.

Natural polysaccharides possess the potential function of masking bitterness due to their three-dimensional network structures. However, they also have relatively high mechanical strength, which is not conducive to swallowing. Taking Coptis chinensis as an example, this study developed a composite gel composed of 0.

Insights from structural characterization for understanding isolation and impact of roasting on allergenic potential of sesame Oleosins.

A systematic extraction protocol involving multiple oil body washing with buffers of NaHCO₃ (pH 10), NaCl, and urea was investigated, and the enrichment of α-helix-rich hydrophobic proteins were monitored by the spectroscopic techniques, while the isolated proteins were identified as oleosin with a relative content of 81.05 %. Dot-blot analysis revealed the allergenicity was enhanced at 150 °C compared to the unroasted state, followed by a decline at 180 °C.

Two-Step Synthesis of a Dolutegravir Intermediate DTG-6 in a Microfluidized Bed Cascade System: Route Design and Kinetic Study.

In the existing two-step method for the preparation of DTG-6 (i.e., an important intermediate of the anti-HIV drug Dolutegravir (DTG)), a strong base is required to neutralize the homogeneous strong acid catalyst of the first step to make the reaction solution weakly acidic for the DTG-5 cyclization in the second step.

Patterning on Living Tardigrades.

Micro/nanofabrication techniques have revolutionized modern photonics and electronics. However, conventional methods remain incompatible with living organisms due to inherent constraints including nonconformal coating, radiation damage, and toxic solvent requirements. Here, we present ice lithography for direct fabrication of micro/nanoscale patterns on the surfaces of tardigrades in their cryptobiotic state.