A Novel Loop-Mediated Isothermal Amplification (LAMP) Assay for Detecting Salmonella Ser. Typhimurium in Egg Products.

Background: As a leading cause of foodborne illness worldwide, detection of Salmonella enterica subsp. enterica serovar Typhimurium is essential for food safety and public health.

Objective: This study aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Salmonella ser.

The Analgesic Effect of NLRP3 Inflammasome in the Relief of Inflammatory Pain by Electroacupuncture: A Systematic Review and Meta-Analysis of Animal Studies.

Introduction: Inflammatory pain originates from the inflammatory response triggered by tissue injury, accompanied by the release of pro-inflammatory mediators. NLRP3 inflammasome, as a key innate immune receptor, plays a central role in inflammatory pain. Electroacupuncture (EA) is a commonly used analgesic method in clinical practice.

Dissecting cross-lineage tumourigenesis under p53 inactivation through single-cell multi-omics and spatial transcriptomics.

Background: Tumour suppressor genes, exemplified by TP53 (encoding the human p53), function as critical guardians against tumourigenesis. Germline TP53-inactivating mutations underlie Li-Fraumeni syndrome, a hereditary cancer predisposition disorder characterised by early-onset pan-tissue malignancies. However, the context-dependent tumour-suppressive mechanisms of p53 remain incompletely elucidated.

Metabolomics navigates natural variation in pathogen-induced secondary metabolism across soybean cultivar populations.

e-induced root rot poses a major threat to soybean production. While the molecular mechanisms underlying soybean- interactions have been extensively studied, their biochemical basis remains largely unexplored. Previous research has identified key metabolic modules involved in pathogen defense, but structural diversity has largely been constrained by studies on single soybean accessions.

MYCN-mediated pyroptosis and inflammation in the molecular mechanism of MicroRNA-202-3P promote functional recovery in spinal cord injury.

Background: Spinal cord injury (SCI), which induces severe neurological dysfunction, triggers secondary damage driven primarily by neuroinflammation and pyroptosis. Although microRNA-202-3P (miR-202-3p) is recognized for its regulatory role in inflammatory pathways, its specific involvement in SCI and the underlying mechanisms remain poorly understood.

Methods: A rat SCI model was induced via T10 spinal cord compression, followed by intravenous miR-202-3p administration.

Source-sink relationship of petroleum hydrocarbons in the oilfield area of Northern Shaanxi: Multilayer perceptron-based modelling systems.

Petroleum contributes significantly to economic growth, but pollution near oilfields poses risks to ecosystems and human health. The soil petroleum content and sources in the Yanchang oilfield, located in northern Shaanxi, remain unclear and present an environmental threat. To address this, a comprehensive soil contamination survey was conducted in the surrounding area.

Luminescence characterization and performance modulation of CaScBO: Eu near-infrared phosphors for night vision lighting applications.

To facilitate the development of near-infrared light sources for photovoltaic and night-vision lighting applications, it is crucial to obtain novel near-infrared emitting phosphors. Herein, a novel Eu-activated broadband near-infrared emitting phosphor CaScBO: Eu is reported. Under 558 nm light excitation, CaScBO: Eu exhibits broadband near-infrared emission centered at 780 nm with a full width at half maxima of 147 nm.

Rb-He excimer involved in four-wave mixing process to produce redshifted collimated blue light.

The four-wave mixing (FWM) process is an important method for generating collimated blue light from alkali vapor. In this paper, we introduced high-pressure He gas to broaden the absorption linewidth of Rb atoms for the pump laser. By using an optical parametric oscillator (OPO) that outputs tunable wavelength pump laser, we performed two-photon excitation of the Rb-He mixture and achieved redshifted and asymmetric broadened collimated light (CL) around 420 nm.

Application of AI-assisted methodology to evaluate diet and ingredient formulations for rodent models in nutrition and health research.

The use of Artificial Intelligence (AI) for peer review is gaining interest by journals and editorial boards because of the length of time required for the scientific peer review process and large numbers of new submissions. The application of AI using a large language model (LLM) like OpenAI's ChatGPT is a valid, rapid means to search published articles that examine diets in rodent studies. The information gathered can be used to evaluate rodent diets and nutrients during peer review or in developing studies and preparing appropriate experimental designs for future nutrition and biomedical research with rodents.

Au-CuS Hierarchical Self-Assembled Multimodal Diagnosis and Treatment Platform for the Synergistic Treatment of Melanoma.

With the rapid development of nontraditional tumor treatment methods, a variety of nanosystems have been successfully developed. Here, a hierarchical self-assembled multimodal diagnosis and treatment platform was designed based on DNA for targeted imaging and treatment of melanoma (B16). First, gold nanorods (AuNRs) and copper sulfide nanoparticles (CuS NPs) were prepared by the seedless growth method and hydrothermal synthesis method, respectively.

Individual and combined effects of polystyrene and divalent cadmium on nitrogen removal performance, microbial community and functional gene in sequencing batch reactors.

This study evaluated the effects of individual and combined polystyrene (PS) and divalent cadmium (Cd) on pollutant removal efficiencies, microbial enzyme activity, microbial toxicity, microbial community structure and nitrogen removal genes in four sequencing batch reactors (SBRs). The results showed that the removal efficiencies of chemical oxygen demand and NH-N in the combined PS and Cd treatment decreased by 12.37 % and 29.

Piezoelectric-Driven Mechanochemical Halogenation: A Transformative Pathway for Sustainable Sandmeyer Reactions via Ball Milling.

The Sandmeyer reaction is a key method for synthesizing aryl halides but often requires complex procedures and large solvent volumes. This study introduces a mechanoredox system using ball milling to activate piezoelectric BaTiO, enabling solvent-minimized, metal-catalyst-free halogenation of aryl diazonium salts under air. The method features a broad substrate scope, scalability, and recyclable piezoelectric materials without significant activity loss.

Identification and behavioral bioassays of alarm pheromones from the onion aphid Neotoxoptera formosana.

Secreted from aphid cornicles, alarm pheromone is an important part of integrated pest management (IPM). As a destructive pest of economic crops, the onion aphid Neotoxoptera formosana threatens the production and quality of allium crops worldwide. However, the limited understanding of the alarm pheromone has hindered their control through IPM strategies.

GPT- 4 as a virtual fitness coach: a case study assessing its effectiveness in providing weight loss and fitness guidance.

Background: As obesity presents a growing public health challenge, demand for personalized fitness solutions has increased. This study evaluates GPT-4's effectiveness as a virtual fitness coach for creating personalized plans.

Method: A 24-year-old Chinese female's data was used by GPT-4 and three professional coaches to develop 16-week fitness plans.

Enantioselective Palladium-Catalyzed Domino Carboetherification Reaction: Access to Isoxazoline-Linked Bis-Heterocycles.

Herein, we present an enantioselective domino carboetherification of β,γ-unsaturated oxime derivatives under mild reaction conditions. This process, catalyzed by palladium in combination with Sadphos ligands, offers a concise and highly efficient route for the assembly of chiral isoxazoline-linked methylenedihydrobenzofuran and methyleneindolin bis-heterocyclic scaffolds. The methodology demonstrates excellent functional group tolerance, a broad substrate scope, and high chemoselectivity and enantioselectivity.

Effect of the implementation of HIV/AIDS prevention and treatment policies on the mortality rate of people living with HIV in Guilin, China.

To analyze the changing trend of HIV/AIDS-related mortality before and after the implementation of the "Prevention and Treatment of HIV/AIDS" policy in Guilin from 1996 to 2020, and to evaluate its effect on HIV/AIDS prevention and treatment. The data of people with HIV reported in Guilin up to December 31, 2020, were obtained from the Chinese Center for Disease Control and Prevention (CDC), and the two rounds of "Prevention and Treatment of HIV/AIDS" policy intervention boundary points were taken in 2010 and 2015, respectively. The interruption time series (ITS) analysis was used to analyze the trend of HIV/AIDS-related mortality before and after the implementation of the HIV/AIDS Prevention and Treatment Project.

Liver metastasis or peritoneal metastasis: single-cell RNA sequencing reveals the organotropism in colorectal cancer is driven by distinct partial-EMT processes.

Liver and peritoneum are the most common metastatic organs in colorectal cancer. However, the mechanisms of metastatic organotropism remain unexplored. Here we used single-cell RNA-sequencing to reveal the intermediate EMT state of tumor cells from colorectal cancer liver metastasis (CRLM) and colorectal cancer peritoneum metastasis (CRPM).

The GSK3β inhibitor attenuate APAP-induced liver injury by regulating PGC1α-mediated oxidative stress.

Acetaminophen (N-acetyl-p-aminophenol, APAP), is the most frequent cause of drug-induced liver injury. Reducing APAP-induced oxidative stress is an effective strategy to improve acute liver injury. Glycogen synthase kinase-3β (GSK3β), is sensitive to redox homeostasis and is involved in oxidative stress-related injuries.

Multi-laboratory validation study of a real-time PCR method for detection of Salmonella in frozen fish.

A multi-laboratory validation (MLV) study was performed to validate a quantitative PCR (qPCR) method developed by the FDA for detecting Salmonella in frozen fish. Frozen fish represents foods that use blending sample preparation procedure in the BAM Salmonella method. This study was carried out after a successful MLV study with baby spinach which represents foods that use soaking sample preparation procedure.

The gut microbiota mediates epoxy eicosanoid metabolism in the colon.

The metabolism of polyunsaturated fatty acids by cytochrome P450 (CYP) mono-oxygenases generates fatty acid epoxides, which are endogenous lipid mediators with potent actions to regulate inflammation, immune responses, vascular tone, and other critical biological processes. While previous research about their biosynthesis has focused on host metabolic enzymes, the role of gut microbiota remains largely unknown. Here, we demonstrate that the gut microbiota directly participates in the metabolism of fatty acid epoxides in the colon by catalyzing their conversion to fatty acid diols, thereby modulating colonic concentrations of these lipid mediators and associated biological actions.

Visible-light-induced radical 1,3-hydrosulfonylation of allylketones with sulfonyl chlorides.

A radical 1,3-hydrosulfonylation of allylketones with sulfonyl chlorides 1,2-carbonyl migration has been achieved under visible-light-irradiation. This reaction proceeds efficiently under mild conditions and exhibits broad substrate scope and excellent functional group tolerance. The synthetic utility of this method is demonstrated by scale-up synthesis and late-stage modification of biologically relevant molecules.

Screening and identification of intestinal lipid-lowering probiotics and their effect on regulating lipid metabolism in C57BL/6J mice.

Recent research shows intestinal microbiota is closely related to the host lipid metabolism. Discovering new lipid-lowering probiotic strains is important for effectively preventing obesity. In this study, five probiotic strains were identified among 108 strains screened from the fecal samples of healthy individuals (18.

Meso-structural evolution of sandstone under uniaxial loading: A study on microdefect compaction and transgranular crack formation mechanisms.

Rock deterioration under uniaxial compression is significantly influenced by changes in meso-structure, which plays a key role in determining the mechanical behavior and stability of rock materials. Understanding how different loading stresses affect the evolution of meso-structure is crucial for assessing rock stability in engineering applications, such as tunneling and landslide prevention. This study investigates the damage mechanisms and meso-structural evolution of sandstone subjected to uniaxial compression at different loading stresses (0, 5, 15, 30, and 40 MPa).

Self-knowledge distillation for prediction of breast cancer molecular subtypes based on digital breast tomosynthesis.

This study aims to investigate the effectiveness of self-knowledge distillation (self-KD) with progressive refinement in the early prediction of molecular subtypes of breast cancer (BC) using digital breast tomosynthesis (DBT) images. This study conducted a retrospective analysis of 368 patients who underwent breast DBT and/or magnetic resonance imaging (MRI) scans at our hospital. Among these patients, 303 underwent DBT scans and 119 underwent MRI scans.