Functional Spermatogenesis Across Testicular Developmental Stages in Neomale Large Yellow Croaker () Revealed by Histology and Gonadal Specific Cellular Markers.

Gonadal development and spermatogenesis critically influence fish reproductive performance. Neomales (genetically female but functionally male) are indispensable for generating all-female populations, yet their spermatogenesis remains understudied. In the present study, we systematically investigated gonadal maturation in neomales of the large yellow croaker (), an economically important marine species exhibiting sexually dimorphic growth.

Research progress and therapeutic strategies in hepatocellular carcinoma metabolic reprogramming.

Background: Hepatocellular carcinoma (HCC) remains a leading cause of cancer-related mortality worldwide, with therapeutic resistance posing a significant clinical challenge. Metabolic reprogramming, a hallmark of cancer, enables tumor cells to alter metabolic pathways to meet their increased energy and biosynthetic demands, playing a critical role in HCC initiation, progression, and drug resistance.

Aim Of Review: This review aims to elucidate the mechanisms underlying metabolic alterations in HCC, including fatty acid, protein, and amino acid metabolism, and to explore their implications in tumor progression and therapeutic resistance.

Sulfonic Acid-Based Covalent Organic Frameworks with Efficient Zn Transport and Storage for Aqueous Zinc-Ion Batteries.

Covalent organic frameworks (COFs) with well-ordered nanopores and numerous accessible redox sites exhibit significant promise in aqueous zinc-ion batteries (AZIBs). However, challenges such as complex synthesis, limited capacity, and poor cycling stability still persist. In this study, we present a Zn preintercalation strategy enabling the one-pot synthesis of long-range interconnected COFs functionalized with -SO groups (COFs-Zn).

Rb-He excimer involved in four-wave mixing process to produce redshifted collimated blue light.

The four-wave mixing (FWM) process is an important method for generating collimated blue light from alkali vapor. In this paper, we introduced high-pressure He gas to broaden the absorption linewidth of Rb atoms for the pump laser. By using an optical parametric oscillator (OPO) that outputs tunable wavelength pump laser, we performed two-photon excitation of the Rb-He mixture and achieved redshifted and asymmetric broadened collimated light (CL) around 420 nm.

Clinical efficacy of magnet-assisted removal for dental high-speed dental handpiece broken Burs in mandibular third molar surgery: a retrospective study.

Background: Breakage and displacement of the high-speed dental handpiece bur is one of the intraoperative complications during mandibular third molar extraction. The retained metallic fragments can trigger issues such as pain and infection. Traditional retrieval methods require extensive tissue dissection, increasing the risk of nerve injury and postoperative complications.

Molecular characterization of the receptor subunits of IL-10 in a perciform fish, Nibea albiflora.

The transmembrane receptor complex of Interleukin-10 (IL-10), comprising the IL-10Rα subunit and IL-10Rβ subunits, belong to the class Ⅱ cytokine receptor family. In the present study, IL-10Rα and IL-10Rβ were characterized in Nibea albiflora (referred to as NaIL-10Rα (MZ620685.1) and NaIL-10Rβ (OK149228.

Cathepsin L-Activatable Fluorescent Probe for Real-Time Monitoring of Drug-Resistant Breast Cancer Cells.

Precise identification of drug-resistant tumor cells remains a critical challenge for optimizing therapeutic strategies and improving clinical outcomes. Herein, we present FR-DCO, a cathepsin L (CTSL)-activatable fluorescent probe, engineered for targeted imaging of doxorubicin-resistant breast cancer cells (MCF-7/ADR). Capitalizing on the overexpression of CTSL in chemoresistant malignancies, FR-DCO integrates a CTSL-specific dipeptide substrate (Phe-Arg, FR) with a dicyanoisophorone (DCO) fluorophore, enabling fluorescence activation via a single-step enzymatic cleavage.

Analysis of the mutation rates and male relative differentiation ability of RM Y-STRs in the Chinese Han population.

Rapidly mutating Y-STRs (RM Y-STRs) have significant application value in differentiating both related and unrelated males. In this study, 26 RM Y-STRs from 367 father-son pairs revealed a total of 224 repeat mutations across all the markers and pairs. The average mutation rate per marker was 18.

Drug treatment of breast cancer brain metastases: progress and challenges.

Breast cancer is one of the most prevalent malignancies among women worldwide, with brain metastasis occurring in approximately 10-16% of cases, significantly contributing to poor prognosis and reduced quality of life. The treatment of breast cancer brain metastasis requires a multidisciplinary approach, prioritizing local therapies such as surgery and radiotherapy to address central nervous system lesions. However, local treatments often struggle to effectively control the progression of brain metastases and are associated with multiple complications, necessitating adjunctive systemic and supportive therapies.

The association between alpha-1-acid glycoprotein and asthma in United States: a cross-sectional study from NHANES.

Background: Alpha-1-acid glycoprotein (AGP), also known as orosomucoid (ORM), is an acute-phase protein associated with asthma. However, research on the relationship between AGP and asthma risk is limited. This study aims to investigate the association between AGP and asthma in the United States.

Potential of bone marrow mesenchymal stem cells in iodine-induced autoimmune thyroiditis therapy.

Objective: Hashimoto's thyroiditis (HT) is a prevalent autoimmune disease without a cure. Mesenchymal stem cells (MSCs) may offer the opportunity to improve autoimmune thyroiditis.

Methods: We replicated the pathogenic factors of HT and established a stable autoimmune thyroiditis model in NOD.

The accelerating effect of sugar on wound healing delayed by bevacizumab: A case report.

Rationale: Bevacizumab, a recombinant humanized monoclonal antibody targeting vascular endothelial growth factor is associated with delayed wound healing. Sugar, a traditional remedy for wound healing, has shown significant efficacy in accelerating recovery. Here, we developed a new strategy for managing bevacizumab-associated delayed wound healing with the topical application of sugar.

Energy dissipation and dilation processes of rock mass under incremental cyclic loading and unloading.

The deterioration of the mechanical properties of rock mass in underground engineering due to energy dissipation and microfracture accumulation under cyclic loading and unloading (CLU) has become a hot research topic in recent years. In order to elucidate the relationship between energy dissipation and the dilation processes, an incremental CLU mode is proposed and employed in triaxial tests in this paper. First, the methods and the procedures of the test were proposed to enhance the success rate, and the triaxial tests under incremental CLU were performed at confining pressures ranging from 0 MPa to 50 MPa.

Macrophage-membrane-engineered NIR II biomimetic nanomaterials for enhanced synergistic chemo-photothermal immunotherapy in cancer treatment.

Nanoparticle encapsulated with PEG-based polymers face limitations in their circulation stability and tumor tissue accumulation during blood transport due to the production of anti-PEG antibodies and their inherent nature as foreign substances, which leads to immune surveillance and clearance by the body. The design of biomimetic nanomaterials based on cell membranes offers a solution to these issues. In this context, we have successfully developed a biomimetic nanomaterials designed for the near-infrared region II (NIR II), which leverage the combined power of chemotherapy and photothermal therapy to activate an immune response against tumors.

Chromosome-level genome assembly and annotation of Chinese herring (Ilisha elongata).

The Chinese herring (Ilisha elongata) is a commercially and scientifically significant fish species. In this study, we conducted high-precision whole-genome sequencing using two high-throughput platforms: second-generation MGI and third-generation PacBio. Hi-C technology assisted in assembling the contig sequences onto 24 chromosomes, resulting in a high-quality chromosome-level genome map with excellent continuity and coverage.

HGH1 Promotes Breast Cancer Progression Through the PI3K/AKT/NF-κB Signaling Pathway: Potential Role for Prognosis and Targeted Therapy.

Introduction: Previous studies have shown that the HGH1 gene is associated with poor prognosis in a variety of cancers, but its specific function and molecular mechanism in the pathological process of breast cancer remain unclear.

Methods: The relationship between expression of HGH1 and overall survival in BC patients was analyzed. Enrichment analysis of HGH1-related signaling pathways and immune infiltration was performed.

Morphological characterization of spermatogenesis and spermatogonial stem cells in Larimichthys crocea, a seasonal breeding teleost†.

Seasonal spermatogenesis in fish is a complex and highly regulated process in which spermatogonial stem cells (SSCs) undergo a series of cellular changes to differentiate into mature sperm. In this study, we systematically described testicular development and identified thirteen different germ cell types throughout the reproductive cycle in large yellow croaker (Larimichthys crocea), a commercially important marine cultured fish in East Asia. Using a set of specific antibodies (VASA, PCNA, DMC1, NANOS2 and GSDF), we developed a high-throughput immunohistochemistry method to identify different types of spermatogenic cells, with a particular focus on distinguishing spermatogonial subtypes.

Screened potential dsRNA targets in the digestive system of adult flea beetle Phyllotreta striolata: psJHBP-like and psaaNAT-like for enhancing RNAi pest control.

Background: The flea beetle Phyllotreta striolata is a major pest of Cruciferae crops, exhibiting strong resistance to chemical pesticides. RNA interference (RNAi) has evolved into an innovative method for managing pests. This study aims to evaluate the potential of spray-induced gene silencing (SIGS) as a management strategy for P.

A gene-based predictive model for lymph node metastasis in cervical cancer: superior performance over imaging techniques.

Objective: Lymph node metastasis (LNM) critically impacts the prognosis and treatment decisions of cervical cancer patients. The accuracy and sensitivity of current imaging techniques, such as CT and MRI, are limited in assessing lymph node status. This study aims to develop a more accurate and efficient method for predicting LNM.

Enabling High Performance in a Positive Potential of Nitrate-to-Ammonia Electrocatalysis Over Mesoporous Core@Shell CuO/Cu@PdCu Nanozyme.

Electrocatalytic tandem nitrate reduction to ammonia (NO -to-NH) offers a promising pathway for energy and environmental sustainability. Although considerable efforts have been presented to modulate the reaction pathways for enhanced NO -to-NH electrocatalysis, these advances often require relatively high overpotentials to balance yield rate and selectivity of NH, resulting in a remarkable energy inefficiency. Inspired by enzyme catalysis in nature, herein a tandem enzyme-like electrocatalyst is designed consisting of a core of CuO/Cu heterojunction surrounded by mesoporous PdCu shell (CuO/Cu@mesoPdCu) that accelerated NO -to-NH electrocatalysis in positive potentials.

Chiral All-Inorganic Perovskite Subnanowires.

The phenomenon of chiral symmetry breaking during the crystallization of achiral molecules or ions, which leads to the formation of controllable enantiomerically pure crystals, has garnered significant interest but remains a challenge to fully overcome. This presents a particularly formidable obstacle in the creation of three-dimensional (3D) structured chiral all-inorganic perovskites, further complicated by their achiral crystalline space groups. In this report, we successfully synthesized right- or left-handed (P/M) chiral 3D P/M-CsPbX (X = Cl, Cl-Br, Br, Br-I) perovskite subnanowires (SNWs), in which Pb(II) can be partially substituted by hetero ions, such as Cu(II), Sn(II), and Mn(II).

Simultaneously enhance nutrient removal and sludge settleability through hydrocyclone-based technology in a full-scale high-inert containing activated sludge process.

Sludge densification technology through hydrocyclone is a promising solution to address the challenge related to increasing loading rate in existing municipal wastewater treatment plants (WWTPs). Although previous studies have investigated the positive effects of hydrocyclone on improving nutrient removal and sludge settleability, little is known if sludge densification technology is still function into the high inert containing activated sludge process in China. This study investigated technical feasibility and revealed underlying mechanisms to simultaneously enhance nitrogen and phosphorus removal and sludge settleability through installing a hydrocyclone-based sludge densification module in a full-scale WWTP with a designed capacity of 4 × 10 m/d.

Cytosolic Monodehydroascorbate Reductase 2 Promotes Oxidative Stress Signaling in Arabidopsis.

The antioxidative enzyme monodehydroascorbate reductase (MDHAR) is represented by five genes in Arabidopsis, including four that encode cytosolic and peroxisomal proteins. The in planta importance of these specific isoforms during oxidative stress remain to be characterised. T-DNA mutants for MDAR genes encoding cytosolic and peroxisomal isoforms were studied.

Effect of adjuvant cisternostomy on the prognosis of patients with severe traumatic brain injury: A single center's retrospective study.

This study evaluates the efficacy of adjuvant cisternostomy (AC) versus decompressive craniectomy (DC) in managing severe traumatic brain injury (sTBI). A single-center retrospective analysis of consecutive sTBI patients treated with AC or DC alone (2018-2019) revealed that the AC group exhibited significantly lower intracranial pressure and higher Glasgow Coma Scale scores compared with the DC group (P < .05).

Physiological effects of sulfadiazine and sulfamethoxazole on Skeletonema costatum and toxicological evaluation using IBR index.

Sulfonamide antibiotics, widely used in human and veterinary medicine as well as agriculture, pose environmental concerns due to their stability and poor biodegradability. This study fills a critical gap in understanding the ecological impact of sulfonamide antibiotics on marine microalgae, particularly Skeletonema costatum, a key primary producer in marine ecosystems. This study investigated the biological responses of the marine microalga Skeletonema costatum to sulfadiazine (SD) and sulfamethoxazole (SMX).