Nanoplastics reshape lipid metabolism in marine microalgae with potential ecological consequence.

Nanoplastics (NPs) in marine ecosystems have garnered increasing attention for their interference with the physiological processes of aquatic organisms. An in-depth examination of the toxicological responses of Nannochloropsis oceanica, a species vital to marine ecosystems, is essential due to the crucial role of lipid metabolism in carbon sequestration and energy allocation in microalgae. This study analyzed the toxicological responses of N.

Discovery of an allosteric 14-3-3 inhibitor for suppressing NRF2-driven cancer phenotypic screening and chemoproteomic-based target deconvolution.

The NRF2 transcription factor is constitutively active in various cancers, functioning as an oncogenic driver for tumor progression and chemo/radiotherapy resistance. Despite the well-documented role of NRF2 overactivation in cancer, no targeted therapy is currently available. In this study, using a combination of phenotypic screening, chemoproteomics, and biochemical and cellular assays, we identified WS3 as a potent allosteric inhibitor of 14-3-3 that selectively inhibits NRF2 activity in tumor cells.

Alterations of plasma neural-derived extracellular vesicles microRNAs in patients with bipolar disorder.

Background: MicroRNAs (miRNAs) alterations in patients with bipolar disorder (BD) are pivotal to the disease's pathogenesis. Since obtaining brain tissue is challenging, most research has shifted to analyzing miRNAs in peripheral blood. One innovative solution is sequencing miRNAs in plasma extracellular vesicles (EVs), particularly those neural-derived EVs emanating from the brain.

Study on the residual dynamics of diazepam in freshwater fish of Zhejiang, China and its implications for human health.

This study provides an in-depth evaluation of diazepam (DZP) and its metabolites in freshwater fish from Zhejiang Province, China, during 2020-2024, with a focus on contamination trends, source attribution, bioaccumulation behavior, and potential human health risks. The results reveal a significant upward trend in DZP detection, rising from 0.33 % to 4.

Symbiosis with and mimicry of corals were facilitated by immune gene loss and body remodeling in the pygmy seahorse.

A remarkable example of symbiosis involves the pygmy seahorse (). It lives obligatorily on gorgonian corals, mimicking their polyps with pink coloration and skin protuberances. Unique for seahorses, pygmy seahorses retain juvenile paedomorphic stunted snouts, resembling the coral's polyps.

Research progress and therapeutic strategies in hepatocellular carcinoma metabolic reprogramming.

Background: Hepatocellular carcinoma (HCC) remains a leading cause of cancer-related mortality worldwide, with therapeutic resistance posing a significant clinical challenge. Metabolic reprogramming, a hallmark of cancer, enables tumor cells to alter metabolic pathways to meet their increased energy and biosynthetic demands, playing a critical role in HCC initiation, progression, and drug resistance.

Aim Of Review: This review aims to elucidate the mechanisms underlying metabolic alterations in HCC, including fatty acid, protein, and amino acid metabolism, and to explore their implications in tumor progression and therapeutic resistance.

Targeting VCAM-1 with chimeric antigen receptor and regulatory T cell for abdominal aortic aneurysm treatment.

Abdominal aortic aneurysm (AAA) is a life-threatening condition characterized by the dilation of the abdominal aorta, leading to a high risk of rupture. Current treatment options are limited, particularly for patients ineligible for surgical interventions. This study explores a novel immunotherapeutic approach using chimeric antigen receptor Treg cells targeting vascular cell adhesion molecule-1 (VCAM-1) to mitigate AAA progression.

Applying Oral Panoramic Radiography to Health Screening of the Aging Population: Cross-sectional and Longitudinal Associations between Adverse Oral Conditions and Intrinsic Capacity Deficiency.

Background: Intrinsic capacity (IC) and oral health are crucial for healthy aging and are linked to future adverse health outcomes. Panoramic radiography provides comprehensive oral assessments and may have broader applications in health screening. However, the cross-sectional and longitudinal relationships between adverse oral conditions detected through panoramic radiographs and deficient IC remain insufficiently explored.

Prediction of parenchymal hematoma after mechanical thrombectomy by asymmetrical prominent veins: a retrospective cohort study based on susceptibility-weighted imaging.

Background: Asymmetrical prominent veins (APVs) can help predict perfusion deficits and collateral circulation in large vessel occlusion acute ischemic stroke. Therefore, this study aimed to investigate the predictive value of APVs for parenchymal hematoma (PH) following mechanical thrombectomy (MT).

Methods: This study retrospectively included consecutive patients with ischemic stroke due to middle cerebral artery occlusion who underwent MT.

Discovery of novel selective CYP1B1 inhibitors: Design, synthesis and biological evaluation.

Cytochrome P450 1B1 (CYP1B1), a key regulator implicated in tumor drug resistance, is aberrantly overexpressed in various malignancies, including non-small cell lung cancer (NSCLC). This study initially elucidated the correlation between CYP1B1 protein levels and paclitaxel resistance in A549 cells using techniques such as Western blotting. Subsequently, leveraging the molecular scaffold of the stilbene-type inhibitor TMS, we employed a computer-aided scaffold hopping strategy to design and synthesize 28 novel derivatives.

A "Signal Relay" Mode Triggered by Bimetallic Synergistic Enhancement in an MOF-Based Multiemission Fluorescence Sensing/Adsorption Platform for Broad-Concentration-Range Tetracycline Antibiotics Visual Detection.

The widespread misuse of tetracycline antibiotics (TCs) and their persistent low-concentration residues in animal-derived foods pose critical threats to human health, while existing analytical methods lack the capability for continuous therapeutic drug monitoring. Herein, a multiemission MOF-on-MOF fluorescence sensing platform codoped with Eu and Zn was engineered via epitaxial growth for simultaneous adsorption and detection of tetracycline, oxytetracycline, chlortetracycline, and doxycycline. By leveraging the differential coordination affinities of Eu and Zn toward TCs, a "signal relay" response mechanism was innovatively designed: At trace concentrations (0.

Fluorescent Three-Dimensional Covalent Organic Frameworks with pcu Topology Based on Triangular Antiprism Node.

The design of highly connected fluorescent polyhedral building blocks is essential for expanding the structural and functional diversity of three-dimensional covalent organic frameworks (3D COFs) but remains largely underexplored due to synthetic and characterization challenges. Here, we report a hexatopic fluorescent building block with triangular antiprismatic geometry, developed via steric-hindrance-directed conformational control and the integration of triphenylamine units. Its imine condensation with linear diamines afforded two crystalline 3D COFs with unambiguously resolved 6-fold Class IIa interpenetrated pcu topology, as determined by continuous rotation electron diffraction.

Prediction of T2/T3 Staging in Patients with Volume-Equivalent Esophageal Squamous Cell Carcinoma on the Basis of PET/CT Radiomics.

Objectives: To develop and externally validate PET/CT-based radiomic models for predicting tumor invasion depth (≤T2 vs. ≥T3) in patients with esophageal squamous cell carcinoma (ESCC) with volume-matched tumors.

Methods: Semiautomatic segmentation was performed on F-FDG PET images, and radiomic features were extracted from PET and coregistered CT scans.

Development of a population of digital brain phantoms for radionuclide imaging research in Parkinson's disease.

Purpose: Dopamine transporter (DAT) SPECT is a powerful tool for early diagnosis of Parkinson's disease (PD), while digital phantoms and Monte Carlo (MC) simulations can serve as important research tools. This study aims to develop a novel digital brain phantom population for Tc-TRODAT-1 (Tc) and I-ioflupane (I) brain SPECT, and to assess attenuation correction (AC) and scatter correction (SC) in DAT SPECT.

Methods: Striatum, brain background (gray and white matter), and cold regions (skull and cerebrospinal fluid) were segmented from 200 T1 MRI brain images from the PPMI dataset.

Hair Metabolomic Profiling of Diseased Forest Musk Deer () Using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS/MS).

Hair, as a non-invasive biospecimen, retains metabolic deposits from sebaceous glands and capillaries, reflecting substances from the peripheral circulation, and provides valuable biochemical information linked to phenotypes, yet its application in animal disease research remains limited. This work applied ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to compare the hair metabolomic characteristics of healthy forest musk deer (FMD, ) and those diagnosed with hemorrhagic pneumonia (HP), phytobezoar disease (PD), and abscess disease (AD). A total of 2119 metabolites were identified in the FMD hair samples, comprising 1084 metabolites in positive ion mode and 1035 metabolites in negative ion mode.

A case report of primary mucinous adenocarcinoma of the thymus and review of the literature.

Thymic mucinous adenocarcinoma is an extremely rare and aggressive malignant tumor of the thymus with a very low 5-year survival rate. Here, we report a case of surgically resected primary mucinous adenocarcinoma of the thymus in a patient in his 60s who was admitted to the hospital with chest tightness for 2 weeks. The patient underwent median sternotomy and thymectomy, including resection of the brachiocephalic vein and partial resection of the superior vena cava wall.

ImmuProgML: machine learning-based dissection of cancer-immune dynamics during tumor progression to improve immunotherapy.

Background: Cancer progression involves distinct stages, with a critical tipping point marking the transition from early to advanced phases, driven by complex tumor-immune dynamics. While immunotherapy has significantly improved outcomes, current biomarker models lack integration of cancer-immune interactions and progression dynamics. Leveraging advances in machine learning, there is an urgent need for a comprehensive framework to systematically analyze these dynamics, predict immunotherapy responses, and improve patient outcomes.

Using Mendelian randomization analyses in osteoarthritis: state of art and future perspectives.

Aims: The etiology of osteoarthritis (OA) remains unclear, involving a complex interaction of environmental and genetic factors. Observational studies have contributed substantially to identify modifiable risk factors that were associated with OA risk, but conclusions about the causality are hard to draw as these observed associations are susceptible to be interfered with reverse causality and residual confounding.

Methods: Mendelian randomization (MR) is a valid approach to provide evidence on causal associations, by using genetic variants as 'proxies' for modifiable exposures to examine the effect of genetic susceptibility to the exposures on the outcomes.

Integrating 16S rDNA sequencing analysis and targeted tryptophan metabolomics to reveal potential mechanism of mulberry leaf extract in treating type 2 diabetes mellitus mice.

This study aims to explore the roles that the interplay between tryptophan (TRP) metabolism and gut microbiota plays in experimental type 2 diabetes mellitus (T2DM) mice treated with mulberry leaf extracts. Fecal and serum TRP metabolites were analyzed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) based targeted metabolomics approaches. Concurrently, gut microbiota composition was characterized through high-throughput sequencing of bacterial 16S rDNA.

Evaluation of real-world CO and NO emissions of various in-use heavy-duty vehicles.

As a major emission source in road transportation, carbon dioxide (CO) emissions from heavy-duty vehicles (HDVs) exacerbate climate change, while nitrogen oxide (NO) emissions pose serious health risks to human health. Therefore, it is critical to investigate in-depth their emission characteristics and evaluate the real-world emission levels of in-use HDVs, as they are an important source of mobile source emission. In this study, eleven in-use HDVs were tested using a Portable Emission Measurement System (PEMS).

Targeting HK3 in tumor-associated macrophages enhances antitumor immunity through augmenting antigen cross-presentation in cervical cancer.

Background: Tumor-associated macrophages (TAMs) are among the most prevalent cells within the tumor microenvironment (TME) of cervical cancer (CC). Although TAMs frequently exhibit an immunosuppressive phenotype, their plasticity enables them as an intriguing reprogrammable target for immunotherapy of CC.

Methods: Consensus clustering was employed to delineate immune infiltration patterns in a cohort of 119 patients with CC.

Facile and eco-friendly fabrication of edible camellia oil body-based propolis oleogels with growth-factor-like properties.

The eco-friendly fabrication of propolis is one of the key bottlenecks that limit the sustainable development of the bee products processing industry. This study successfully developed a straightforward and environmentally benign camellia oil-based propolis oleogel that leverage the "lipophilic" properties of the growth-factor-like components in propolis. The critical gelation concentration was determined to be 4 %.

Genetic Erosion in Captive Alpine Musk Deer Highlights the Challenges of Conserving Endangered Species in Closed Populations.

The alpine musk deer () is a critically endangered species in China whose wild populations have precipitously declined due to habitat degradation and poaching. In response, China established the world's first captive breeding facility for this species in 1990. Despite over three decades of closed breeding, the genetic consequences of long-term captivity remain unclear.

Pre-strain and release in MOF catalysts: Ligand-engineered strain storage for enhanced catalytic activity.

The strategic exploitation of structural tension effects in heterogeneous catalyst design remains underexplored, particularly in programmable frameworks. Here, we engineer a bipyridyl-based metal-organic framework (MOF) platform (-CH, H, -CF) anchoring Cu(OTf)₂ for regioselective ring-opening of epoxides, demonstrating how programmable ligand geometry controls catalysis via strain effects. Both CH- and CF-modified MOFs show significantly enhanced catalytic activity, achieving turnover frequencies 3.

Rapid Differential Diagnosis of Influenza A and B Viruses With RT-RPA Centrifugal Microfluidic Chip.

Influenza A H1N1 and Influenza B are two of the most prevalent airborne viruses, capable of triggering seasonal epidemics that affect millions of individuals globally. Rapid on-site identification of these two viruses is crucial to accurate clinical diagnosis and prompt control of public health risks. This study presents a novel one-step reverse transcription recombinase polymerase amplification (RT-RPA) method integrated with a centrifugal microfluidic chip for rapid differential diagnosis of Influenza A H1N1 and Influenza B viruses.