Nanoplastics reshape lipid metabolism in marine microalgae with potential ecological consequence.

Nanoplastics (NPs) in marine ecosystems have garnered increasing attention for their interference with the physiological processes of aquatic organisms. An in-depth examination of the toxicological responses of Nannochloropsis oceanica, a species vital to marine ecosystems, is essential due to the crucial role of lipid metabolism in carbon sequestration and energy allocation in microalgae. This study analyzed the toxicological responses of N.

Alternative Splicing of the NMDA Receptor Subunit GluN1 Mediated by Polypyrimidine Tract-Binding Protein Dimerization in the Trigeminal Ganglion Contributes to Orofacial Allodynia.

Orofacial neuropathic pain, a debilitating condition associated with trigeminal nerve injury, is often characterized by allodynia. N-methyl-d-aspartate receptors (NMDARs), particularly the GluN1 subunit, play a central role in mediating this pain. The GluN1 subunit undergoes alternative splicing at exon 5, generating isoforms GluN1a (lacking the exon 5-encoded N1 cassette) and GluN1b (retaining the N1 cassette), which have distinct functional roles.

Research progress of NLRP3 in immune cells of systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) represents a multifaceted autoimmune disorder characterised by widespread organ involvement and abnormal autoantibody production. Its underlying mechanisms are closely related to immune system dysfunction. Recent advancements in immunological research have highlighted the pivotal role of the NLRP3 inflammasome, which acts as a key regulator of both innate and adaptive immunity.

Lithium Exposure Causes Trophoblast Cuproptosis by Upregulating FOXO1/STEAP4 Axis in Unexplained Miscarriage.

Lithium (Li) batteries have been used worldwide, but few are recycled, thus, the waste Li has been widely spread into the environment and finally accumulated in human body. Cuproptosis is a newly reported Cu-dependent and programmed cell death form. The unclear pathogenesis of unexplained miscarriage (UM) largely restricts its clinical treatment and global human reproduction.

Cysteine mediates the association of essential trace elements with polycystic ovary syndrome: Does ambient particulate matters exposure modify the mediation effect of cysteine.

Objective: In this study, we aimed to investigate the relationship between essential trace element levels in FF and PCOS and to determine whether this association was mediated by the antioxidant stress indicator cysteine (Cys). In addition, the effect of ambient PM exposure on the mediation effect of Cys was examined.

Methods: A case-control study was conducted, which involved 420 patients with PCOS and 408 controls.

EGFR Targeted Liposomal PROTAC Assisted With Epigenetic Regulation as an Efficient Strategy for Osimertinib-Resistant Lung Cancer Therapy.

Osimertinib resistance is a global problem for NSCLC patients mediated by new EGFR mutations or bypass mechanisms. Proteolysis targeting chimeras (PROTACs) have been utilized to overcome drug resistance by degrading mutant EGFR, but most are restricted to their poor cell permeability and insufficient tumor-targeting ability. Meanwhile, these PROTACs has little effect on bypass resistance mechanisms.

Cost-effective micro/mesoporous biochar derived from soda residue-assisted hydrocarbonization and activation of crop waste for sulfamethoxazole removal from water environments.

The low-cost transformation of agricultural biowaste into high-value biochar materials presents substantial technological and economic challenges. In this study, we report for the first time that soda residue can effectively substitute for chemical activation agents to produce micro/mesoporous biochars (MMBs) with a well-developed specific surface area (473.73 m g) and pore volume (0.

Lycium barbarum glycopeptide and luteolin synergistically protect mouse photoreceptors against N-nitroso-N-methylurea-induced degeneration.

Photoreceptor degeneration is a major cause of vision impairment in retinal diseases, for which no effective treatment currently exists. Previous research by our team demonstrated that Lycium barbarum glycopeptide and luteolin can independently promote photoreceptor survival and function in degenerated mouse retinas, although with limited efficacy. This study evaluated whether a combination of Lycium barbarum glycopeptide and luteolin provides enhanced therapeutic benefits compared with either compound alone.

Melatonin Protects against LPS-Induced Mitochondrial Dyshomeostasis and Ovarian Damage through JNK Signaling Pathway in Mouse Ovary.

Both mitochondrial dysfunction and inflammation are closely associated with the pathogenesis of diminished ovarian reserve (DOR). While melatonin (MT) is known to protect against ovarian injury, its precise mechanism in counteracting lipopolysaccharide (LPS)-induced mitochondrial dysfunction and ovarian reserve impairment remains unclear. This study aimed to explore the effects and underlying mechanisms of MT on LPS-induced ovarian reserve dysfunction.

Passive social media use and depression among college students during public health emergencies: a chain mediation approach.

Introduction: Despite growing evidence linking passive social media use to depression during public health emergencies, the underlying psychological mechanisms remain unclear. This study investigates the impact of passive social media use on depression among college students during the COVID-19 pandemic, specifically examining the mediating roles of vicarious traumatization and fear of missing out (FoMO).

Methods: A structured survey was conducted among 322 college students.

Traditional medicine Kuanxiong Aerosol alleviates atherosclerotic plaque inflammation by mitigating CX3CR1 macrophage apoptosis via the cGAS-STING pathway.

Background: Atherosclerosis (AS), a chronic inflammatory vascular disease, represents the leading cause of lethal cardiovascular events and demands innovative therapies targeting plaque inflammation. Kuanxiong Aerosol (KX), a clinically used traditional Chinese medicine for angina, exhibits potent anti-inflammatory activities.

Purpose: This investigation aimed to explore the therapeutic potential and mechanism of KX for AS.

Folate mediates cognitive impairment of aged people with periodontitis.

Background: Periodontitis is associated with poorer cognitive function. It remains unclear whether folate plays a crucial role in periodontitis-promoted cognitive impairment (CI) in the elderly population.

Methods: Data for this cross-sectional population-based study were obtained from the National Health and Nutrition Examination Survey (NHANES), 2011-2014 database.

DLP 3D printing of hyperelastic photocurable perivascular scaffolds enabling patient-specific vascular remodeling.

Photopolymerization-based 3D printing, enabled the fabrication of complex, patient-specific scaffolds with high resolution and spatial precision. However, most photocurable biomaterials exhibited mechanical mismatch with soft tissues such as vascular, cartilage, and tendon tissues. To address this limitation, we developed a biodegradable and elastomeric resin (A-PLCL/4SH), composed of methacrylate-functionalized poly(L-lactide-co-ε-caprolactone) (A-PLCL) and pentaerythritol tetra(3-mercaptopropionate) (PETA-4SH), which enabled high-fidelity digital light processing (DLP) 3D printing.

Preconception paternal exposure to zearalenone impairs male fertility and IVF embryo survival in mice.

Zearalenone (ZEA), a fungal mycotoxin commonly found in various human foods, exhibits a structural similarity to naturally occurring estrogen, raising significant concerns regarding its reproductive toxicity. This study aimed to assess the impact of preconceptional ZEA exposure on male reproductive health and early embryonic development. Healthy B6D2F1 male mice were exposed to corn oil or varying doses of ZEA for two weeks.

Effects of accelerated intermittent theta-burst stimulation combined with cognitive training on brain function in amnestic mild cognitive impairment patients.

Objective: This double-blinded randomized trial investigated the effects of accelerated intermittent theta-burst stimulation (iTBS) and adaptive cognitive training on brain function in 24 patients with amnestic mild cognitive impairment (aMCI).

Methods: Participants received real (n=12) or sham (n=12) iTBS targeting the left dorsolateral prefrontal cortex (DLPFC) thrice daily alongside cognitive training for 14 days. The Montreal Cognitive Assessment (MoCA), Alzheimer's Disease Assessment Scale-Cognitive Subscale (ADAS-Cog), and functional near-infrared spectroscopy (fNIRS) during rest and N-back tasks, which measure cognitive function and brain activity respectively, were administered pre-and post-intervention.

Natural variation of AcEGY3 mediates chloroplastic ROS homeostasis to confer kiwifruit thermotolerance.

The genus of Actinidia consists of the popular kiwifruits consuming worldwide. Most kiwifruit species are naturally distributed in warm and moist environments. So, they are vulnerable to high levels of heat stress.

miR-423-3p inhibits CTNNBIP1/WNT preventing hyperandrogenic PCOS.

Polycystic ovary syndrome (PCOS) lacks the generally accepted diagnostic biomarkers and targeted therapy. Increasing evidence indicates that microRNAs (miRNAs) play a crucial role in PCOS. Hereby, we tested the functional implications of a novel miRNA (miR-423-3p) as a mediator in the progress of hyperandrogenic PCOS, as well as its potential as a new serum biomarker and therapeutic target for the PCOS.

CDDO-imidazolide ameliorates sepsis-induced ARDS by enhancing mitophagy via the Nrf2 pathway to prohibit alveolar macrophage pyroptosis and HMGB1 release.

Accumulating evidence suggests that NLRP3-mediated alveolar macrophage (AM) pyroptosis and subsequent high mobility group box protein 1 (HMGB1) secretion play significant roles in the pathogenesis of acute respiratory distress syndrome (ARDS). Nrf2 has been shown to be individually involved in regulating pyroptosis. In this study, we investigate the ability of CDDO-imidazolide, a potent Nrf2 activator, to regulate AM pyroptosis and HMGB1 secretion in sepsis-associated ARDS, along with its underlying mechanism.

Triplet Regimen of Metronomic Capecitabine Plus Antiangiogenic Drug and PD-1 Inhibitor as Later-Line Salvage Treatment for Patients With MSS/pMMR Metastatic Colorectal Cancer: A Retrospective Study.

Later-line treatment has demonstrated limited survival benefits in patients with metastatic colorectal cancer (mCRC). This retrospective study evaluated the efficacy and safety of a triplet regimen combining metronomic capecitabine, antiangiogenic drugs, and PD-1 inhibitors in patients with mCRC. Between January 2021 and December 2023, 21 patients with mCRC received a triplet regimen as later-line treatment.

The GmbHLH13-GmCHS7 module positively regulates isoflavones accumulation in soybean (Glycine max. L).

Isoflavonoids are widely regarded as phytoalexins and plant estrogens, with applications in plant defense and human healthcare. However, the mechanism of soy isoflavone synthesis remains unclear. In this study, we identified a gene from Glycine max, designated as basic helix-loop-helix 13 (GmbHLH13), which is a member of the bHLH transcription factor family.

MSC-Derived Extracellular Vesicles: Roles and Molecular Mechanisms for Tissue Repair.

Mesenchymal stem cells (MSCs) are pluripotent stem cells that have great potential in the field of regenerative medicine. Extracellular vesicles that derived from MSCs inherit the bioactive molecules, including lipids, proteins, nucleic acids, from the donate cells. With similar biological functions as MSCs, MSC-EVs show lower toxicity, better biocompatibility, stability, and less immune rejection.

Ultrasonic Cavitation-Manipulated Macrophages Hitchhiking Augments Delivery of Nanocarriers for Active and Efficient Cancer Therapy.

Cell-derived vehicles have shown promise in drug delivery for cancer therapy, but they are hindered by lack of effective methods to release drug from cellular vehicles. To overcome this dilemma, this work develops nanodrug-hitchhiking macrophages (OGNLN@MACs) that can unload drugs under ultrasound irradiation. The OGNLN is perfluoropentane-loaded liposomal nanodroplet which consists of lipophilic prodrugs of elaidate-conjugated gemcitabine and NLG919.

Theoretically improved biodegradability of fluoroquinolones in sewage and sludge.

Fluoroquinolones (FQs), recognized for their potent antibacterial activity, have been extensively used in clinical and veterinary medicine. However, their low removal efficiency during sewage treatment and the emergence of drug resistance have raised significant concerns. In this study, a molecular "design-screening-validation-mechanism analysis" approach was developed to design FQ alternatives with improved removal efficiency and reduced drug resistance.

Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening.

Background: Lung cancer exhibits a high degree of heterogeneity, rendering its treatment one of the most difficult challenges. Current laboratory lung cancer models cannot fully preserve the diversity of the disease and predict drug responses. Owing to the rarity of human-derived samples, transgenic mouse models can be extensively used in fundamental research.

Modified laminoplasty versus laminectomy with fusion in different K-line status among patients with high occupation rate of cervical ossification of longitudinal ligament.

Background Context: For patients with high occupation rate cervical ossification of the posterior longitudinal ligament (HOR-cOPLL) that are K-line negative, laminoplasty often results in poor outcomes due to postoperative loss of cervical curvature. Preserving the posterior muscle-ligament complex (PMLC) on the hinge side during laminoplasty has been shown to reduce cervical curve loss and improve postoperative cervical alignment. We hypothesized that a modified laminoplasty (mLP) with preserving the PMLC could maintain cervical curvature and achieve similar neurological recovery to that of laminectomy with fusion (LF), regardless of K-line status.