Genotype-Phenotype Correlation in Fibrous Dysplasia/McCune-Albright Syndrome Patients With Craniofacial Lesions.

Objectives: We aimed to investigate genotype-phenotype correlations, variant prevalence, and prognostic factors in a Chinese cohort with craniofacial FD/MAS.

Methods: A retrospective study of 93 histologically confirmed FD/MAS cases (2003-2024) analyzed GNAS mutations via direct sequencing. Clinical data, including disease onset, lesion activity (active vs.

[Application of the jigsaw classroom method in oral histology and pathology course].

Purpose: This study applied jigsaw classroom method to reform some contents of oral histology and pathology course, aiming at exploring new methods of undergraduate education in stomatology, achieving more dimensional learning goals and improving learning effects.

Methods: Twenty-four undergraduates from the School of Stomatology of Fudan University were selected as the study objects. Under the guidance of teachers, students studied oral squamous cell carcinoma cases online and offline for 3 weeks.

Dimension-Tunable Supramolecular Organic Frameworks with Chiral Pore Channels for Selective Guest Encapsulation and Enhanced Circularly Polarized Luminescence.

Here, we report a dimension-tunable supramolecular organic framework (SOF) with chiral pore channels, which shows selective guest encapsulation and enhanced circularly polarized luminescence (CPL). SOFs from a chiral para-substituted bis-naphthalene bis-urea (PNU) are fabricated by both vapor diffusion crystallization and an antisolvent self-assembly approach, leading to the formation of one-dimensional (1D) micrometer-sized single crystal and two-dimensional (2D) plate-like and three-dimensional (3D) octahedral self-assembled microstructures, respectively. The similar permanent porosity of these dimension-tunable chiral SOFs is collectively proved by N adsorption-desorption experiments, single-crystal structure, high-resolution transmission electron microscopy (HR-TEM), and density functional theory (DFT) calculations.

Extracellular Vesicles of Salivary Adenoid Cystic Carcinoma Suppress Immune Microenvironment in Lung.

Objective: Salivary adenoid cystic carcinoma (SACC) frequently metastasizes to the lung and affects patient survival. Extracellular vesicles (EVs) have significant effects in the tumor pre-metastatic niche (PMN). In this study, we aimed to investigate immune changes in the lung pre-metastatic microenvironment of SACC.

Swept-Source Optical Coherence Tomography Angiography for Retinal Neurovascular Structural Evaluation in a Chronic Unpredictable Mild Stress Rat Model.

Purpose: The purpose of this study was to evaluate the retinal thickness and vascular changes in a chronic unpredictable mild stress (CUMS) rat model using swept-source optical coherence tomography angiography (SS-OCTA).

Methods: Eight-week-old male Sprague Dawley rat models of CUMS (n = 11) and age-matched control rats (n = 12) were imaged by a customized SS-OCTA system. Two groups were imaged after modeling stimulation for 12 weeks.

[Progress in clinicopathological diagnosis of oral potentially malignant disorders].

As the field of oral pathology has evolved, the nomenclature and classification of oral mucosal diseases with a remarkable risk of malignant transformation have undergone several modifications. In 2005, the World Health Organization (WHO) introduced the concept of oral potentially malignant disorders (OPMDs) as an alternative to the terms for oral precancerous lesions and precancerous conditions. In the consensus report by the WHO Collaborating Center for Oral Cancer of 2021, OPMD is defined as "any oral mucosal abnormality that is associated with a statistically increased risk of developing oral cancer.

Inactivation of RhoA for Hypertension Treatment Through the TRPV4-RhoA-RhoGDI1 Axis.

Background: The RhoA (Ras homolog family member A) signaling pathway is pivotal in regulating vascular smooth muscle cells (VSMCs) function and blood pressure homeostasis. Current inhibitors of the RhoA signaling pathway are limited in hypertension treatment, suffering from poor efficacy, insufficient specificity, and developmental challenges.

Methods: Cryo-electron microscopy (EM), proximity ligation assay (PLA), and site-directed mutagenesis were used to explore the mechanism of RhoA activity regulation.

Integrating Dynamical Systems Modeling with Spatiotemporal scRNA-Seq Data Analysis.

Understanding the dynamic nature of biological systems is fundamental to deciphering cellular behavior, developmental processes, and disease progression. Single-cell RNA sequencing (scRNA-seq) has provided static snapshots of gene expression, offering valuable insights into cellular states at a single time point. Recent advancements in temporally resolved scRNA-seq, spatial transcriptomics (ST), and time-series spatial transcriptomics (temporal-ST) have further revolutionized our ability to study the spatiotemporal dynamics of individual cells.

MLIF inhibits inflammation and maintains intestinal flora homeostasis in a dextran sulfate sodium (DSS)-induced colitis mouse model.

Ulcerative colitis (UC) is a chronic inflammatory disease primarily affecting the colon, characterized by mucosal inflammation and ulceration. Monocyte locomotion inhibitory factor (MLIF), a heat-stable pentapeptide derived from Entamoeba histolytica, has demonstrated the anti-inflammatory capacity. The aim of the current work was to test the protective effects of MLIF in a dextran sulfate sodium (DSS)-induced colitis mouse model.

Benthic foraminiferal community structure and its response to heavy metals revealed using environmental DNA/RNA metabarcoding in Yueqing Bay, East China Sea.

Foraminifera are among the most important components of benthic communities widely used as indicators of marine environmental changes. Determining the effect of heavy metal pollution on benthic foraminifera is a key requirement for evaluating the health of coastal ecosystems. In this study, environmental DNA (eDNA) and RNA (eRNA) metabarcoding was used to evaluate the benthic foraminiferal community structure and diversity in Yueqing Bay, East China Sea.

Monthly Variation, Environmental Drivers, and Ecological Functions of Marine Bacterial Community in a Eutrophic Coastal Area of China.

This study investigated the monthly variations of bacterial communities in the surface seawater of the Wenzhou coastal area and their influencing factors, while exploring the ecological functions of microbial communities. The results indicated that the surface seawater bacterial communities in this region exhibited high diversity, with significantly higher diversity observed in the winter half-year compared to the summer half-year. The bacterial community structures showed distinct monthly variations, with high similarity between adjacent months, particularly from June to September.

Spectral stripe-based large-range chromatic confocal displacement system with nanometer uncertainty.

We present a novel, to the best of our knowledge, design of a chromatic confocal displacement system (CCDS) based on spectral stripe. In this system, confocal light collected by the dispersive lens is shaped by a Powell lens and then diffracted onto a detector in the form of a thin straight stripe. The displacement variant is converted into the change of stripe positions on the detector.

Profiling heavy metals distribution in surface sediments from the perspective of coastal industrial structure and their impacts on bacterial communities.

Heavy metal pollution of marine sediments along the coastal industrial parks have always received extensive attention due to their persistent hazard to local marine ecosystem. Despite this, our knowledge about the influence of geography and coastal industrial structures on heavy metal distributions remains little. In this study, surface sediment samples were collected from the coastal zone of the industrial park in Ningbo.

HEGM: Hierarchical Ensemble Generation Model for nuclear reaction cross sections generation.

Accurate and reliable nuclear reaction cross section data are crucial for nuclear reactor design, nuclear energy development, and nuclear safety assessments. Traditional methods for generating nuclear data face significant challenges, including high costs, long time frames, and incomplete coverage. Recent advances in machine learning (ML) offer new opportunities for nuclear data generation, but existing methods struggle with the scarcity of experimental data, limiting their ability to generate high-precision and broadly applicable cross section data.

Clinical Characteristics and Prognostic Factors of 199 Salivary Acinic Cell Carcinoma.

Objects: This retrospective study aimed to investigate the clinical characteristics and prognostic features of acinic cell carcinoma (AciCC) of the salivary gland.

Methods: One Hundred Ninety-nine cases of AciCC were retrospectively reviewed. Clinical characteristics and outcomes were systematically collected.

Distribution characteristics and source analysis of dissolved organic matter with different molecular weights in the coastal waters of Zhejiang Province.

Dissolved organic matter (DOM) is a crucial component of the carbon cycle in ecosystems, with its composition, functional groups, and chemical structures varying significantly depending on source and molecular weight (MW). In this study, the content, composition characteristics, and sources of DOM in the coastal waters of Zhejiang Province were investigated using total organic carbon analysis, ultraviolet-visible (UV-Vis) absorption spectroscopy, and excitation-emission matrix (EEM) spectroscopy combined with parallel factor analysis (PARAFAC). The results indicated regional differences in the content and composition of DOM.

The Multi-Target Action Mechanism for the Anti-Periodontitis Effect of Based on Bioinformatics Analysis and In Vitro Verification.

is a traditional Chinese medicine with potential therapeutic effects on periodontitis; however, its underlying mechanisms require further investigation. We employed network pharmacology, molecular docking, molecular dynamics simulations, and in vitro experiments to explore the potential actions and mechanisms of in treating periodontitis. A total of 17 compounds (including the most prevalent one, Kaempferol) from and 464 corresponding targets were identified, from which five major active ingredients were selected based on the drug-active ingredient and periodontitis gene network.

Physiological effects of sulfadiazine and sulfamethoxazole on Skeletonema costatum and toxicological evaluation using IBR index.

Sulfonamide antibiotics, widely used in human and veterinary medicine as well as agriculture, pose environmental concerns due to their stability and poor biodegradability. This study fills a critical gap in understanding the ecological impact of sulfonamide antibiotics on marine microalgae, particularly Skeletonema costatum, a key primary producer in marine ecosystems. This study investigated the biological responses of the marine microalga Skeletonema costatum to sulfadiazine (SD) and sulfamethoxazole (SMX).

Total flavonoids isolated from can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats.

Ethnopharmacological Relevance: , recognized as a traditional Chinese medicinal herb, can tonify liver and kidney and strengthen bones and muscles. Modern pharmacological research has proved that could prohibit the occurrence of osteoporosis and arthritis.

Aim: To investigate the effect and action mechanism of total flavonoids isolated from the leaves of (TFEL) on bone loss in ovariectomized (OVX) rats, and to study its effect on intestinal flora.

Locally Delivered Hydrogel with Sustained Release of Flavonol Compound Kaempferol Mitigates Inflammatory Progression of Periodontitis and Enhances the Gut Microflora Composition in Rats.

Objective: This study aimed to investigate the effects of a sustained-release composite containing gelatin methacryloyl (Gel) and kaempferol (Ka, K) on experimental periodontitis symptoms in rats.

Methods: Forty 6-week-old male rats were randomly assigned to four treatment groups in a specific pathogen-free (SPF) environment: Control group (C), periodontitis model group (M), Gel alone group (G), and Gel_Ka composite-treated group (G_K). Treatment effects on the periodontal status of bilateral maxillary second molars in each rat group were assessed by micro-CT imaging and histology.

Supramolecular rosette intermediated homochiral double helix.

Precise organization of organic molecules into homochiral double-helix remains a challenge due to the difficulty in controlling both self-assembly process and chirality transfer across length scales. Here, we report that a type of bisnaphthalene bisurea molecule could assemble into chirality-controlled nanoscale double-helices by a supramolecular rosette-intermediated hierarchical self-assembly mechanism. A solvent-mixing self-assembly protocol is adopted to direct bisnaphthalene bisurea cyclization into chiral discrete rosettes through cooperative intramolecular and intermolecular hydrogen bonds.

Assessment of responses of cultured benthic foraminiferal communities to copper pollution through environmental RNA metabarcoding analysis.

Copper (Cu) is an essential trace element and can accumulate in sedimentary environments. However, beyond a certain concentration threshold, Cu can pose toxicity risks to biota. Benthic foraminifera are widely distributed and exhibit characteristics such as high biodiversity, short life cycle, good preservation potential, and high pollutant sensitivity, making them good bioindicators.

Aspartate Metabolism-Driven Gut Microbiota Dynamics and RIP-Dependent Mitochondrial Function Counteract Oxidative Stress.

Aspartate (Asp) metabolism-mediated antioxidant functions have important implications for neonatal growth and intestinal health; however, the antioxidant mechanisms through which Asp regulates the gut microbiota and influences RIP activation remain elusive. This study reports that chronic oxidative stress disrupts gut microbiota and metabolite balance and that such imbalance is intricately tied to the perturbation of Asp metabolism. Under normal conditions, in vivo and in vitro studies reveal that exogenous Asp improves intestinal health by regulating epithelial cell proliferation, nutrient uptake, and apoptosis.

The Impact of on Oral and Systemic Health: A Comprehensive Review of Recent Research.

Oral diseases, particularly dental caries and periodontal disease, pose significant global health challenges. The imbalance of the oral microbiota plays a key role in the occurrence of these diseases, prompting researchers to seek new strategies to restore oral ecological balance. is a Gram-positive rod-shaped bacterium that exists in various body parts of humans, including the gastrointestinal tract, urinary tract, skin, and so on.