CuSO protects hepatocytes from lipotoxicity by promoting autophagic degradation of lipid droplets to attenuate oxidative stress.

Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) is a prevalent chronic liver condition that often progresses to Metabolic Dysfunction-Associated Steatohepatitis (MASH), ultimately leading to liver fibrosis and hepatocellular carcinoma (HCC). Consequently, the early diagnosis and treatment of MASLD are particularly crucial. Currently, the pathogenic mechanisms underlying MASLD remain incompletely understood, and there is no optimal therapy available for this condition. The liver serves as the primary organ for copper storage, maintaining copper levels within a stable range under physiological conditions. Both copper deficiency and excess can result in cellular damage and liver dysfunction. In this study, we demonstrate that hepatic copper deficiency is closely associated with the pathogenesis of MASLD. Furthermore, we found that CuSO influence the progression of MASLD by modulating the Chaperone-Mediated Autophagy (CMA) pathway, thereby restoring the autophagic degradation of lipid droplets, reducing oxidative stress, enhancing cell viability, and alleviating lipid deposition. In summary, by investigating the potential relationship between intrahepatic copper deficiency and MASLD, we have explored the protective role of copper ions in hepatocytes and laid the groundwork for developing molecular markers for diagnosing and treating MASLD.