Advances in Research on Monogenic Hereditary Hypertension.

Hypertension is one of the most significant preventable health risk factors globally. Among its various forms, monogenic hypertension, although rare, warrants attention. As awareness of monogenic hypertension grows within both the public and medical communities, research efforts are intensifying.

Quality Evaluation of Literature Reports on Clinical Randomized Controlled Trials of Acupuncture Treatment for Stable Angina Pectoris.

Objective: To evaluate the reporting quality of randomized controlled trials on acupuncture for the treatment of stable angina pectoris.

Methods: A systematic search was conducted in both Chinese and English databases, including CNKI, Wanfang, VIP, SinoMed, PubMed, Embase, Web of Science, and the Cochrane Library, with a focus on studies published from the inception of each database to March 4, 2025. This search aimed to identify clinical RCTs exploring the effectiveness of acupuncture in treating stable angina pectoris.

Detection of phosphatidylserine exposure on Mycoplasma hyorhinis by flow cytometry.

Mycoplasma often contaminates eukaryotic cell cultures. Determining Mycoplasma cell viability is important when evaluating the cellular response to drug treatment. One method to test cell viability is using a membrane-impermeant nucleic acid dye that is generally excluded from viable cells.

Macrophage-derived VISTA engages with LRIG1 and hinders gut epithelial repair in colitis.

Disruption of the intestinal epithelial barrier and incomplete repair are critical for the development of colitis. V-domain immunoglobulin domain suppressor of T-cell activation (VISTA), encoded by Vsir, functions as an immune checkpoint. In the present work, we report that VISTA is predominantly upregulated in macrophages from patients with inflammatory bowel disease (IBD) and in mice with dextran sulfate sodium (DSS)-induced colitis.

Molecular landscape, subtypes, and therapeutic vulnerabilities of central nervous system solitary fibrous tumors.

Solitary fibrous tumors (SFTs) of the central nervous system (CNS) are rare, aggressive mesenchymal neoplasms with high recurrence and metastasis rates. Here, we perform a comprehensive multi-omics analysis of 189 cases of CNS SFTs integrating 94 whole genome sequencing, 88 transcriptomics, 7 single-nucleus RNA sequencing and 3 spatial transcriptome sequencing. We find that receptor tyrosine kinase mutations are significantly more prevalent besides the widespread NAB2-STAT6 fusion and correlate with tumor grade.

Celastrol-Loaded Targeted Antioxidative Nanozyme for Improving Lipid Metabolism and the Renal Microenvironment in Diabetic Nephropathy.

Oxidative stress and abnormal lipid metabolism in podocytes and renal tubules are closely associated with the progression of diabetic nephropathy (DN). Conventional therapeutic agents for DN exhibit limited clinical efficacy due to inherent drawbacks such as poor target selectivity, short half-life, and unfavorable physicochemical properties. Therefore, the development of a safe and efficient targeted therapeutic strategy for DN has become urgently necessary.

Iron‑catalyzed divergent synthesis of carbazole-based di-/triarylmethanes.

A simple and efficient iron-catalyzed divergent dehydrative coupling of carbazoles and benzylic alcohols has been developed. By using different iron catalysts, mono-substituted or di-substituted carbazole-based di-/triarylmethanes, some of which are bioactive and potential pharmaceutical molecules or potential molecular precursors for optoelectronic materials, were synthesized site-selectively from the same set of reactants. This method was demonstrated with 68 examples, in up to 98% yield and with broad functional group compatibilities.

A novel hybrid approach for quantitative source apportionment and source-oriented health risk of heavy metal(loid)s pollution in an old industrial city.

Heavy metal(loid)s (HMs) contamination in soil is a major environmental problem worldwide due to its threat to the environment and human health, particularly in old industrial cities. However, the accurate identification of sources, drivers and risks of HMs remains to be further studied. In this paper, a novel hybrid approach combining self-organizing map (SOM), Positive Matrix Factorization (PMF), Gradient Boosting Decision Tree (GBDT) and Monte Carlo simulation was applied to evaluate quantitative source identification, driving factors and source-oriented health risks of HMs in an old industrial city.

[Occurrence Characteristics and Ecological Risk Assessment of Microplastic Pollution in the Yellow River Basin].

The Yellow River, as the "mother river" of China, urgently needs research and analysis on the MPs pollution status and ecological risks in the Yellow River Basin. The aim of the study was to explore the spatial distribution and composition characteristics of MPs in the Yellow River Basin, establish a comprehensive investigation and evaluation system for MPs at the watershed scale, and based on a two-dimensional risk assessment matrix, evaluate the absolute ecological risk of MPs at each sampling point within the watershed. The results showed that the abundance range of MPs in the study area was （0.

HERV-K TM Subunit Elicits CD8 T Cell Anergy and Tumor Immune Evasion via Targeting CD3 Coreceptor ε in AML and PDAC.

CD8 T cell anergy is a critical driver of cancer immune evasion, but the underlying causes and mechanisms remain elusive. Here, the functional human endogenous retroviruses-K envelope (HERV-K Env) subunit transmembrane (K-TM) is identified as a potent viral immune checkpoint that induces CD8 T cell anergy and elicits immune evasion in acute myeloid leukemia (AML) and pancreatic duct adenocarcinoma (PDAC). K-TM subunits are highly expressed in CD8 T cells and enriched in sera of cancer patients.

Comparison of Haploidentical-Hematopoietic Stem Cell Transplantation Using Modified Post-Transplantation Cyclophosphamide and Eltrombopag Combined With Immunosuppression for the Treatment of Severe Aplastic Anemia.

First-line treatments for severe aplastic anemia (SAA) patients include HLA-matched sibling donor hematopoietic stem cell transplantation (MSD-HSCT) for young patients and immunosuppressive therapy (IST) for those without suitable donors or with advanced age. Nevertheless, MSD-HSCT is limited by donor availability, while IST is associated with high relapse and clonal evolution risks. Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) is commonly recommended as salvage therapy for SAA patients following primary IST failure.

Insight into pericytes in glioblastoma angiogenesis: In vivo tracking by two-photon microscopy and proteomic profiling.

Background: Glioblastoma (GBM) is a highly aggressive brain tumor characterized by aberrant angiogenesis and an immunosuppressive microenvironment. Pericytes are aberrantly recruited but their spatiotemporal roles and molecular changes remain unclear. This study investigated platelet-derived growth factor receptor beta-positive (Pdgfrb) pericyte dynamics and reprogramming in GBM vasculature.

[Effect of heterologous expression of malic enzyme gene on photosynthetic carbon fixation and lipid accumulation in tobacco leaves].

Microalgae possess high photosynthetic efficiency, robust adaptability, and substantial biomass, serving as excellent biological resources for large-scale cultivation. Malic enzyme (ME), a ubiquitous metabolic enzyme in living organisms, catalyzes the decarboxylation of malate to produce pyruvate, CO, and NAD(P)H, playing a role in multiple metabolic pathways including energy metabolism, photosynthesis, respiration, and biosynthesis. In this study, we identified the malic enzyme gene () and its biological functions, aiming to provide excellent target genes for the genetic improvement of higher plants.

Sphk2 in ischemic stroke pathogenesis: Roles, mechanisms, and regulation strategies.

Ischemic stroke, a leading cause of mortality and long-term disability worldwide, is characterized by acute cerebral artery occlusion leading to neuronal death and functional deficits. Despite advances in reperfusion therapies, the lack of effective neuroprotective agents underscores the need for novel therapeutic strategies targeting secondary injury mechanisms. Sphingosine kinase 2 (Sphk2) has emerged as a pivotal regulator in ischemic stroke pathogenesis, mitigating blood-brain barrier leakage, neuroinflammation, and neuronal survival through its downstream metabolite, sphingosine-1-phosphate.

How to Implement Advance Care Planning for People With Dementia: A Scoping Review.

Background: Advance care planning for people with dementia is an important process to ensure that patient preferences are respected throughout disease progression. However, the complexity of advance care planning and the challenges in effective communication hinder its implementation. The lack of clear procedural guidance for health care teams and the limited research on practical issues such as building trust and resolving conflicts further complicate this process.

A new method for detecting mycoplasma in cell cultures using colocalization of DNA and membrane staining.

Mycoplasma often surreptitiously contaminates cell cultures without detection, mainly parasitizing the cell surface and thus interfering to some extent with the cells. Direct DNA staining of cell cultures often yields equivocal results and will only reliably detect heavily contaminated cultures. Interpretation is difficult in cases where degraded DNA from host cells may produce small spots of fluorescence under microscopy that mimic mycoplasma.

Promoting Social Participation in Cognitive Decline: A Systematic Review and Meta-Analysis of Intervention Effectiveness and Behavior Change Mechanisms.

Background: Cognitive decline, including subjective cognitive decline (SCD), mild cognitive impairment (MCI), and dementia, significantly affects social participation, leading to social isolation and reduced quality of life. Enhancing social participation through interventions may mitigate these effects, yet evidence on intervention effectiveness and mechanisms remains inconsistent.

Aims: To evaluate the effectiveness of social participation interventions for individuals with cognitive decline and identify effective behavior change techniques (BCTs) supporting social participation.

Effects of telemedicine interventions on cognitive function in post-stroke cognitive impairment: A systematic review and meta-analysis.

BackgroundPost-stroke cognitive impairment is characterized by cognitive dysfunction occurring within 6 months post-stroke. Telemedicine uses communication technologies to deliver healthcare remotely and has shown efficacy in improving cognitive impairment. However, a systematic review specifically evaluating telemedicine's effects on cognitive outcomes in post-stroke cognitive impairment is lacking.

Overexpression of the ABA Synthesis Gene OsABA2 Enhances Seed Storability in Rice.

Seed storability is critical for seed production and germplasm conservation. Numerous studies have linked seed storability to abscisic acid (ABA) metabolism; however, the use of biotechnological approaches to strategically and rapidly enhance seed storability in rice through manipulation of ABA biosynthesis genes remains unexplored. In the current study, we developed overexpression lines (OE) of OsABA2, which encodes a XANTHOXIN DEHYDROGENASE, a key enzyme in the ABA synthesis pathway.

Condition-controlled divergent trifluoroalkylation: a diversity-oriented synthesis strategy for efficient construction of CF-decorated carbazole libraries.

A condition-controlled divergent trifluoroalkylation strategy for carbazole derivatives has been developed via alkylation with trifluoropyruvate. Employing trifluoroacetic acid (TFA) promoter, FeCl catalyst, or AgSbF catalyst, a wide scope of mono-trifluoromethylated carbazolylethanols (27 examples, up to 99% yield), di-trifluoromethylated carbazolylethanols (16 examples, up to 93% yield), and trifluoromethylated bis(carbazolyl)propionates (8 examples, up to 76% yield) were efficiently and selectively synthesized respectively for the first time. The features of these transformations include (1) precise control over product divergence through systematic condition modulation, (2) efficient construction of three distinct trifluoromethylated architectures from identical substrates, and (3) operational simplicity under mild reaction conditions.

GRIN2B alleviates mid-gestational sevoflurane exposure-induced early differentiation of rat neural stem cells by interacting with KIF17.

General anesthetic exposure during pregnancy has neurotoxic effects on the developing brain, causing long-term cognitive dysfunction in the offspring. Sevoflurane exposure during mid-gestation results in premature differentiation of neural stem cells (NSCs), being the crucial factor affecting normal hippocampal functions and contributing to neurocognitive impairment. However, the related molecular mechanism remains unclear.

Human induced neural progenitor cells generated from three-dimensional aggregate-based culture significantly improve post-stroke recovery in tMCAO mice.

Background: Despite the high prevalence of cerebral ischemic stroke, effective clinical treatments remain limited. With the development of regenerative medicine, induced neural progenitor cells (iNPCs) demonstrate ideal potential and good availability for autologous transplantation therapy. However, current differentiation protocols for iNPCs still have room for improvement in terms of purity, reproducibility, scalability and differentiation potential.

Effects of Lipid Metabolites and Cross Organ Lipid Metabolism Regulation on Bone Homeostasis.

Osteoporosis is a metabolic bone disease characterized by the loss and destruction of bone microstructure. Its incidence rate increases with age and is an important public health problem. Recently, the relationship between lipid metabolism and osteoporosis has received widespread attention.

The role of lncRNA-ZFAS1 in liver fibrosis: insights into the miR-1953/TAZ axis.

Long non-coding RNA-Zinc finger antisense 1 (lncRNA-ZFAS1) is involved in the progression of several cancer types, yet its function in liver fibrosis remains unclear. The purpose of this study was to examine ZFAS1 expression in liver fibrosis and explore its possible molecular mechanism. ZFAS1 expression was measured in a liver fibrosis model and in activated hepatic stellate cells (HSCs).