Mediating effect of moral resilience between good-death perception and coping with death competence of ICU nurses: a cross-sectional study.

Background: Moral resilience can help Intensive Care Unit (ICU) nurses overcome moral dilemmas caused by the death of patients, while enhancing their competence to cope with death. Death-coping competence is also related to the cognitive level of nurses about good death. However, few studies have focused on the relationships among between moral resilience, perception of good death, and death-coping competence.

Dual . isolated anti-Ro antibody positivity in rheumatoid arthritis.

Objective: This study aimed to evaluate the clinical and immunological significance of anti-Ro52/TRIM21 and anti-Ro60/SSA antibodies in rheumatoid arthritis (RA), particularly the association of dual antibody positivity with disease severity, systemic manifestations, and therapeutic resistance.

Methods: We conducted a cohort study involving 670 RA patients, stratified into four groups according to anti-Ro52 and anti-Ro60 antibody status: Ro52+/Ro60+, Ro52+/Ro60-, Ro52-/Ro60+, and Ro52-/Ro60-. Clinical characteristics, disease activity scores (DAS28-ESR, DAS28-CRP), systemic complications, and treatment responses were compared among groups.

Subpopulation-specific apoptotic responses of hemocytes to decapod iridescent virus 1 in Macrobrachium rosenbergii.

Decapod iridescent virus 1 (DIV1) is an emerging viral pathogen responsible for high mortality in cultured Macrobrachium rosenbergii. Despite the pivotal role of hemocytes in shrimp antiviral immunity, the subpopulation-specific apoptotic dynamics in response to DIV1 remain unclear. In this study, we integrated flow cytometry and single-cell RNA sequencing to dissect hemocyte responses during DIV1 infection.

Mendelian randomization and single-cell analysis reveal causal relationships between renal clear cell carcinoma, kidney fibrosis, and inflammatory factors.

Background: Renal clear cell carcinoma (RCC) is the most common type of kidney cancer, and its relationship with kidney fibrosis and inflammatory responses has attracted considerable attention. However, whether causal relationships exist among these associations remains unclear, as traditional observational studies are susceptible to confounding factors. To evaluate causal relationships between kidney cancer, kidney fibrosis, and inflammatory factors using Mendelian randomization, and explore tumor microenvironment heterogeneity through single-cell analysis.

Glycolysis to lactylation: Unraveling the metabolic and epigenetic landscape in tissue fibrosis (Review).

Tissue fibrosis represents a pathological condition characterized by excessive accumulation of extracellular matrix (ECM) components. Although historically considered a byproduct of glycolysis, lactate has emerged as a key signaling molecule influencing diverse physiological and pathological processes, including fibrosis. Roles have emerged for lactate metabolism and lactylation, a novel post‑translational modification, in regulating fibroblast activation, ECM deposition and fibrotic progression.

diversity from karst area in Yunnan, Shilin, and four new species.

spp. are widely distributed across diverse environments and play a significant role in both ecosystem stability and economic applications. In this study, 57 strains were isolated from karst desert soil, of which 47 strains were identified as nine known species, while 10 strains were characterized as belonging to four novel species.

Investigating the Role of Lactate-Related Genes in Radiotherapy Resistance of Lung Cancer by Integrated Bioinformatics and Experiment Validation.

Radiotherapy is a standard treatment for advanced lung cancer, but resistance remains a significant cause of treatment failure. This study aimed to investigate lactate-associated genes to identify patients likely to benefit from radiotherapy. RNA-seq data from 99 patients with lung cancer who underwent radiotherapy were analyzed to identify differentially expressed genes (DEGs) between resistant and sensitive cases.

LncRNA SNHG14 drives angiotensin-induced abdominal aortic aneurysm progression by regulating autophagy via the miR-27b-3p/Wnt/β-catenin/c-Myc axis.

Abdominal aortic aneurysm (AAA) is a global health concern associated with high mortality. It has been reported that long noncoding RNAs (lncRNAs) are involved in the development of AAA. In this study, our objective was to investigate the roles of lncRNA small nucleolar RNA host gene 14 (SNHG14) and the associated mechanisms in the development of AAA.

The role of innate lymphoid cells in kidney disease.

Innate lymphoid cells (ILCs) are crucial components of the innate immune system that facilitate rapid responses to tissue homeostasis, infection, injury, and inflammation. Recent studies have shown that ILCs can migrate from intestinal tissue to the kidneys, where they mediate immune function, playing an important role in the pathogenesis of acute and chronic kidney diseases. This review addresses the latest research progress on ILCs in mucosal immunity and acute and chronic kidney diseases, focusing on the potential mechanisms of ILCs in the pathogenesis and progression of diseases, such as acute kidney injury, immunoglobulin A (IgA) nephropathy, and lupus nephritis.

Correlation study on the risk of cardiovascular adverse events in diabetic foot patients based on machine learning - a retrospective cohort study.

Introduction: Diabetic Foot (DF), as a serious complication of diabetes, is closely related to major adverse cardiovascular events (MACE) and mortality. However, research on predictive models for the MACE risk in DF patients is not sufficient. The purpose of this study is to construct a prognostic model for the MACE risk in patients with diabetic foot ulcers and provide a reference tool for clinical individualized management.

Dual positivity for Anti-Ro52 and Anti-Ro60 antibodies is linked to greater organ involvement and disease progression in systemic sclerosis.

Objectives: To investigate the distinct and combined effects of anti-Ro52/TRIM21 and anti-Ro60/SSA antibodies on the clinical features and prognosis of patients with systemic sclerosis (SSc).

Methods: A single-center observational study was conducted using data from the Renji Scleroderma Longitudinal Cohort (Renji-SLOC), including 379 SSc patients with at least 1 year of follow-up. The patients were categorized into four groups based on serological status: double-negative (Ro52/Ro60), isolated anti-Ro52/TRIM21 positive (Ro52), isolated anti-Ro60/SSA positive (Ro60), double-positive (Ro52/Ro60).

Ma Xing Shi Gan Decoction alleviates lipopolysaccharide-induced pneumonia by inhibiting NLRP3 inflammasome activation via AMPK/mTOR/ULK1-mediated autophagy.

Ethnopharmacological Relevance: Pneumonia represents a common acute respiratory infection that presents a major health concern worldwide. The Ma Xing Shi Gan Decoction (MXSG) is a famous formulation utilized for respiratory ailments for millennia and celebrated for its impressive therapeutic benefits. However, the specific mechanism of MXSG alleviating pneumonia remains unclear.

Innovative emulsification and freeze-drying techniques for developing heat-resistant chocolates with tunable textures and sensory attributes.

The development of heat-resistant chocolate that meets customer preferences has been a longstanding challenge for both industry and academia. This study presented an innovative method for producing freeze-dried (FD) chocolate with enhanced heat resistance by integrating emulsification and freeze-drying technologies without altering the chocolate composition. Cryo-electron microscopy and X-ray microscopy revealed a unique microstructure in the product, where water-soluble components, such as sugar, formed a matrix that encapsulated fat-soluble components such as cocoa butter, providing heat resistance up to 70 °C.

Physical fitness status and associated determinants among Chinese children aged 9-12 years in Shandong province: a population-based cross-sectional study.

Children's physical fitness is a critical determinant of lifelong health, yet comprehensive investigations into its multi-level influencing factors remain limited, particularly in developing regions. Guided by the Ecological Model of Health Behavior, this cross-sectional study assessed the physical fitness status and associated determinants among 29,856 children aged 9-12 years in Shandong Province, China. Data were obtained from two primary sources: (1) standardized physical fitness assessments based on the National Physical Fitness Standards for Students of China (Revised 2014), and (2) validated questionnaires capturing individual behaviors, family environments, and lifestyle factors across multiple ecological levels.

[Analysis of a neonate with Hypoparathyroidism-sensorineural deafness-renal dysplasia syndrome and a literature review].

Objective: To analyze the phenotype and genotype of a neonate with Hypoparathyroidism-sensorineural deafness-renal dysplasia syndrome (HDR).

Methods: A female neonate with HDR syndrome and thyroid deficiency detected at the First Affiliated Hospital of Zhengzhou University on December 6,2023 was selected as the study subject, Low-coverage whole-genome sequencing (Lc WGS) and whole exome sequencing (WES) were carried out. Using "hypoparathyroidism""sensorineural deafness""renal dysplasia""HDR""Barakat" and"GATA3" as keywords, the CNKI, Wanfang Data Knowledge Service Platform and PubMed database were searched, and the retrieval time was set from the establishment to March 2025.

Co-fermentation of and enhances the flavor characteristic of mature coconut water: Insights from volatile and non-volatile profiles.

The mature coconut water (MCW) is rich in nutrients, but its sour and astringent taste makes it unsuitable for direct consumption. In this study, a co-fermentation system employing and was developed to enhance the flavor of MCW, wherein an indigenous 1-7-1 was screened for optimal sensory characteristics when co-fermented with A33. Co-fermentation changed odor and taste characteristics of MCW with a broad range of organic compounds and the high richness taste attribute.

Analysis and verification of potential ferroptosis-related diagnostic markers in the early stage of metabolic dysfunction-associated steatohepatitis.

Metabolic dysfunction-associated steatohepatitis (MASH) is currently the fastest-growing cause of liver-related deaths. Intervention in the early stage of MASH will effectively reverse the condition and avoid huge health and economic burdens. To date, our understanding of MASH remains quite limited.

Exploring the alterations in microstate dynamics during the migraine cycle and detecting pre-ictal phases.

Background: Microstate analysis captures brief but critical fluctuations in brain activity, making it a powerful tool for exploring the cyclic nature of migraine. In this study, we aimed to investigate microstate features during different migraine phases and develop a classification model to identify the pre-ictal phase.

Methods: From May 2023 to June 2024, we conducted a cross-sectional study with consecutive recruitment, collecting resting-state electroencephalography data from 174 individuals with migraine without aura and 50 healthy controls, followed by classification of migraine phases.

Immunofocusing design of a fusion glycoprotein monomer vaccine for respiratory syncytial virus.

Respiratory syncytial virus (RSV) can cause severe lower respiratory tract infections in infants and the elderly. Previously, most RSV vaccine antigen design strategies have focused on the stability of the prefusion (PreF) protein trimer conformation. We hypothesized that the RSV monomer might serve as a vaccine candidate immunogen.

Stromatolites and pulsed oxygenation events in the Mesoproterozoic Longjiayuan formation of western Henan: evidence for life-environment co-evolution.

The oxygen level in the ocean and atmosphere played a crucial role in the development of Precambrian stromatolites. The Mesoproterozoic represents a critical interval in Earth's history, characterized by persistently low atmospheric and oceanic oxygen levels under which stromatolites flourished. Stromatolites are particularly abundant in Member II of the Mesoproterozoic Longjiayuan formation in western Henan Province.

Extracellular vesicles enhance the efficacy of ceftiofur against intracellular bacterial infections.

Bacterial infections pose a major threat to human health and economic stability. In particular, intracellular bacterial infections present significant clinical challenges due to antibiotic resistance and poor drug penetration. Therefore, there is an urgent need to develop novel therapeutic strategies to address the problem of intracellular bacterial infections.

A High-Calorie Diet Aggravates Lipopolysaccharide-Induced Pulmonary Inflammation in Juvenile Rats via Hypothalamic-Pituitary-Adrenal Axis-Related Pathways.

The hypothalamic-pituitary-adrenal (HPA) axis plays an important regulatory role in inflammatory responses to systemic or local infection in the host. A high-calorie diet, which can aggravate pediatric pneumonia and delay recovery, is intimately associated with HPA axis disorder; however, its underlying mechanisms remain unknown. This study examined whether the mechanism by which a high-calorie diet aggravates pneumonia is related to HPA axis disorder.

Emp3 promotes the progression of renal fibrosis in mice with unilateral ureteral obstruction by activating TGF-β/Smad3 signaling pathway.

Background: Epithelial membrane protein 3 (Emp3), an N-glycosylated tetraspanin, is known to regulate tumor progression; however, its role in renal fibrosis (RF) remains unclear. This study aims to elucidate the function of Emp3 in RF.

Methods: To evaluate Emp3's role, we utilized electroporation to deliver the Emp3-targeting RfxCas13d knockdown plasmid into the kidney cells of C57BL/6 mice, followed by the well-established unilateral ureteral obstruction (UUO) model.

High-speed imaging of CNT deagglomeration in aqueous solution with surfactant.

This study investigates the mesoscale deagglomeration mechanisms of multi-walled carbon nanotubes (MWCNTs) in aqueous solutions with and without added surfactant (Triton X-100), using high-speed imaging and numerical simulations. High-speed observations revealed that within the cavitation zone (CZ, defined as the region of high bubble intensity), the addition of surfactant had no obvious effects on deagglomeration behaviour, with most agglomerates remaining intact and only occasional fragmentation events observed. In contrast, in regions outside the CZ, surfactant addition significantly increased the number and stability of microbubble clusters, leading to more frequent interactions with MWCNT agglomerates.

PCSK9 inhibitor improved cardiac function after acute myocardial infarction in rats.

Background: This study investigated the effects and possible mechanisms of Protein expression of protein convertase subtilisin/kexin type 9 (PCSK9) inhibitor evolocumab on cardiac function in rats with acute myocardial infarction (AMI).

Methods: The AMI model was established by ligating the left anterior descending coronary artery in rats. The mRNA expression of PCSK9 in myocardial tissues was detected by real-time fluorescent quantitative PCR (RT-qPCR).