Partial Reprogramming in Senescent Schwann Cells Enhances Peripheral Nerve Regeneration via Restoration of Stress Granule Homeostasis.

Partial reprogramming (pulsed expression of reprogramming transcription factors) ameliorates multiple tissue functions in aged mice; however, its impact on peripheral nerve regeneration remains largely unexplored. In this study, the temporal dynamics of Schwann cells following sciatic nerve injury in young and aged rats are systematically examined using single-cell transcriptomics to identify a Runx2 cell population highly enriched with stress granules as transitional homeostatic cells during Schwann cell differentiation. It is found that pathological accumulation of this cluster during axonal regeneration constitutes a critical contributing factor to impaired neural repair in aging.

Disruptions of morphological brain networks and their associations with multi-symptoms in children with spastic cerebral palsy.

Background: Spastic cerebral palsy (SCP) is associated with extensive alterations in regional cortical morphology. However, the specific effects of SCP on the topological organization of morphological brain networks remain largely unknown. This study aimed to investigate these effects and explore their potential correlations with clinical manifestations in SCP children.

Epidemiological investigation of porcine in pig herds in Guangxi, China (2022-2023) and genetic diversity analysis based on multilocus sequence typing.

Introduction: Mycoplasma hyopneumoniae (Mhp) infection significantly challenges Guangxi's pig farms, yet its prevalence and molecular characteristics remain poorly understood. This study aimed to define circulating Mhp genotypes and their distribution in the region.

Methods: From 2022-2023, 1,362 pig lung samples were randomly collected from 14 Guangxi regions.

MiR-181d-5p affects skin wound healing processes via the Ikbkg/NF-κB axis.

The skin, a vital barrier against environmental damage, plays a crucial role in maintaining organismal homeostasis. However, the molecular mechanisms underlying skin wound repair remain incompletely understood, with microRNA regulation potentially serving as a key regulatory component. Utilizing the amphibian-derived cyclic peptide Cy as a molecular probe, we identified the miR-181d-5p/Ikbkg/NF-κB axis as significantly associated with wound healing through small RNA sequencing of early-stage skin injury.

Ultrasound and genetic findings in a case series of fetuses presenting vertebral defects.

Purpose: This study aimed to summarize the ultrasound and genetic features in a case series of fetuses presenting vertebral defects, to provide useful information for prenatal counseling and prognostic evaluation.

Methods: Fetuses with vertebral anomalies by a second or third trimester ultrasound screening between January 2020 and April 2024 at a single center were included in the study. Chromosome microarray analysis (CMA) as a first-line diagnostic test was performed.

Identification and validation of anoikis-related differentially expressed genes in nasopharyngeal carcinoma.

Background: Anoikis resistance is a critical feature enabling cancer cells to survive during detachment from the extracellular matrix. This study aimed to identify and validate anoikis-related differentially expressed genes (ARDEGs) in nasopharyngeal carcinoma (NPC), providing new insights into the molecular mechanisms underlying NPC progression and potential therapeutic targets.

Methods: Four gene expression datasets from the Gene Expression Omnibus (GEO) database were integrated to form the GEO-Combined dataset.

Prenatal Characterization of Houge-Janssens Syndrome Type 2: A Case Report and Systematic Review of Fetal Phenotypes Associated With PPP2R1A Mutations.

Background: Houge-Janssens syndrome type 2 (HJS2, OMIM 616362) is a rare neurodevelopmental disorder caused by pathogenic variants in PPP2R1A, typically characterized postnatally by hypotonia, developmental delay, intellectual disability, and distinctive craniofacial features.

Methods: We describe a 28-year-old pregnant woman referred for increased nuchal translucency (4.4 mm) and high risk on first trimester screening.

Genome-wide identification of family genes in sweet potato ( L.) and their expression patterns under abiotic stress.

GATAs, a type of zinc finger protein transcription factors, can bind to DNA regulatory regions to control the expression of target genes, thereby affecting plant growth and development under normal conditions or environmental stress. However, the gene family has not been identified in sweet potato. In this study, a total of 35, 33, 34, 39, 63, and 56 genes were identified in sweet potato, , , , , and , respectively.

Functionalizing walnut protein via consecutive purification and ultrasound modification: Self-assembled structure and interfacial properties.

The preparation of plant proteins generally involves three independent steps, which is time-consuming. Here, we integrated these three steps into one consecutive process (one-step preparation), which involved alkali extraction, membrane purification, and ultrasound modification. The obtained walnut protein was evaluated in terms of self-assembled structure, interfacial behaviors, and emulsifying properties.

Interleukin 21-Armed EGFR-VHH-CAR-T Cell Therapy for the Treatment of Esophageal Squamous Cell Carcinoma.

Esophageal squamous cell carcinoma (ESCC) is a common form of esophageal cancer with a poor prognosis and limited treatment options. Epidermal growth factor receptor (EGFR), an overexpressed oncogenic gene in all ESCC patients, is an attractive target for developing therapies against ESCC. There is an extremely urgent need to develop immunotherapy tools targeting EGFR for the treatment of ESCC.

Risk factors for surgical site infections following microwave ablation of the uterus: a retrospective cohort study.

Purpose: As microwave ablation (MWA) becomes an increasingly popular treatment for gynecological diseases, complications such as surgical site infections (SSIs) have emerged as critical concerns, particularly in high-risk patient populations. Identifying independent risk factors for SSIs is essential to implement effective preventive measures and enhance patient outcomes.

Methods: This retrospective study included women aged 18-55 with a diagnosis of uterine myoma or adenomyosis who underwent MWA at the First Affiliated Hospital of the University of Science and Technology of China between December 2020 and November 2023.

Structural and functional constraints on spike activation and host protease utilization limit cell entry of SARS-CoV-2-related bat coronaviruses.

Unlabelled: The persistent threat posed by SARS-CoV-2-related coronaviruses (SC2r-CoVs) in wildlife highlights the risk of zoonotic transmission. Cross-species infectivity is predominantly determined by spike (S) character and S-mediated cell entry. In this study, we systematically investigated BANAL-52 and BANAL-103, which exhibit the closest genetic proximity to SARS-CoV-2, focusing on their spike structures and functional characteristics.

Mechanistic Insights into CYP2A6 Inactivation by Visnagin and Its Impact on Pharmacokinetic Properties of Tegafur.

Visnagin (VNG), a furanochromone, is a major active component of the plant (L.) Lam often used for the preparation of tea products. This study aims to comprehensively investigate the mechanism of VNG-mediated CYP2A6 enzyme inactivation and the effects of VNG on the pharmacokinetics of the antitumor drug tegafur.

Influencing factors of self-reported near-miss in clinical nursing information system tasks among nurses: a multicenter cross-sectional study.

Background: Clinical nursing information systems have certain safety vulnerabilities in the complex interactions of the healthcare environment. Near-misses refer to patient safety incidents that have the potential to cause harm but do not result in adverse outcomes, serving as frontline sentinels for identifying risks. The aim of this study was to assess the current status of near misses in clinical nursing information system tasks and the factors that influence them, to provide insights for improving patient safety and enhancing care management.

Association of inactive pulmonary tuberculosis lesions with the risk of active disease development in the elderly: a population-based retrospective study.

Background: Individuals with inactive pulmonary tuberculosis (PTB) lesions were found to be high-risk populations for active PTB development. This retrospective study evaluated the association between different types of inactive PTB lesions and the development of active PTB aiming to provide epidemiological evidence for developing precise intervention strategies.

Methods: Based on a population-based PTB active-case-finding project conducted on the elderly, 154 028 subjects who had participated in the 2020 baseline survey were included in the current analysis.

Identification and validation of a prognostic signature of mC-related genes for esophageal cancer.

Background: Esophageal cancer (ESCA) is one of the most common malignancies, and has a poor prognosis. The role of 5-methylcytosine (mC) regulators has been linked to carcinogenesis in a number of cancers. However, the role of mC regulators in ESCA is unclear.

Novel TMEM53 missense variant generated a new ubiquitination site and cause Craniotubular dysplasia, Ikegawa type.

Genetic mutations in the TMEM53 gene have been linked to Craniotubular Dysplasia, Ikegawa Type (CTDI). To elucidate the etiology in a consanguineous family exhibiting the typical clinical phenotype of CTDI, trio whole exome sequencing (Trio-WES) was conducted, and a homozygous missense variant in the TMEM53 gene (NM_024587.4: c.

Emerging epidemic of the predominant clusters of FC428-like Neisseria gonorrhoeae in Guangdong, China.

Objectives: This study aimed to investigate the molecular characteristics of predominant epidemic clusters of FC428-like N. gonorrhoeae in Guangdong, China in 2022.

Methods: Minimum inhibitory concentrations (MICs) were determined for N.

Combined oxybenzone and UVB exposure induces metabolic and neural dysregulation in Mugilogobius chulae embryos and larvae.

The widespread use of sunscreens has contaminated aquatic environments with UV filters like oxybenzone (BP-3), which act as endocrine disruptors in aquatic organisms and exhibit enhanced toxicity under UV radiation. This study employed a multi-omics approach integrating responses from genes to whole organisms to investigate UVB-potentiated toxicity of BP-3 in embryos of the marine fish Mugilogobius chulae. Results demonstrated that UVB significantly amplified BP-3 toxicity, reducing LC from 5674 μg L⁻¹ to 3653 μg L⁻¹ and impairing key developmental endpoints: spontaneous movement, heart rate, and body length.

Recycled Aggregates for Sustainable Construction: Strengthening Strategies and Emerging Frontiers.

The transformative trajectory of urban development in the contemporary era has engendered a substantial escalation in construction waste generation, particularly in China, where it constitutes approximately 40% of the total solid waste stream. Traditional landfill disposal methodologies pose formidable ecological challenges, encompassing soil contamination, groundwater pollution, and significant greenhouse gas emissions. Furthermore, the unsustainable exploitation of natural sandstone resources undermines energy security and disrupts ecological balance.

The effect of disease uncertainty on stress perception among parents of critically ill children in the PICU:the mediating effect of coping styles.

Objective: To investigate the mediating role of coping styles in the relationship between disease uncertainty and stress perception among parents of critically ill children in the PICU.

Methods: This cross-sectional study recruited 273 parents of children admitted to the PICU of a tertiary hospital in Guangdong Province, China. Standardized scales were used to assess disease uncertainty, coping style, and perceived stress.

Electrochemical Synthesis of 5-Amino-1,2,4-thiadiazoles from Elemental Sulfur, Isocyanides, and Amidines.

1,2,4-Thiadiazoles, an important five-membered heterocyclic ring containing nitrogen and sulfur, are widely present in various functional molecules. Hence, a facile electrochemical three-component reaction of isocyanides, elemental sulfur, and amidines is reported for the construction of 1,2,4-thiadiazoles. A series of 5-amino-1,2,4-thiadiazoles are prepared in a simple undivided cell at room temperature with good functional group tolerance and high atom economy.

8-Epidiosbulbin E acetate triggers apoptosis via metabolic activation-mediated crosstalk between mitochondria and endoplasmic reticulum.

8-Epidiosbulbin E acetate (EEA), a furan-containing diterpenoid lactone and key component of Dioscorea bulbifera L., has been found to induce notable liver injury in mice through CYP3A-mediated metabolic activation to a cis-enedial reactive metabolite. EEA exposure at 25, 50, 100, or 200 µM triggered apoptosis in cultured mouse primary hepatocytes, evidenced by alterations in mitochondrial and endoplasmic reticulum (ER) pathway protein levels.

Metabolic activation and cytotoxicity of bavachin mediated by CYP3A in mice.

Bavachin (BVC), a flavonoid, is found in Psoraleae fructus (PF) which has been reported to induce various adverse effects, particularly hepatotoxicity, such as increases of serum alanine transaminase (ALT) and aspartate transaminase (AST) in mice given BVC. However, the mechanisms underlying its hepatotoxicity remain unclear. During the incubation of mouse microsomes with BVC in the presence of glutathione (GSH) or N-acetylcysteine (NAC), one oxidative metabolite (M1), one GSH conjugate (M2), and one NAC conjugate (M3) were observed.