The Role of Methylmalonic Acid in the Risk of Sarcopenia and All-Cause Mortality Among Individuals With Sarcopenia: Evidence From NHANES.

Mitochondrial dysfunction is increasingly recognized as a driver of sarcopenia pathogenesis, progression, and prognosis. Muscle mass is a fundamental and objective component of sarcopenia. In some studies, relative muscle loss has been used to define sarcopenia.

Rapid screening of glyphosate in environmental water based on Ponceau 4R-Cu competitive complexation.

Glyphosate, a widely used organophosphorus herbicide in agriculture, poses potential threats to aquatic ecosystems and human health due to its long-term environmental persistence. This study presents a spectroscopic detection system based on a competitive reaction utilizing the Ponceau 4R (P4R)-Cu complex. Leveraging glyphosate's high affinity for chelating copper ions, the method enables indirect, rapid, and visual quantitative analysis of glyphosate.

Anti-PS IgG Immune Complexes Impair Macrophage Phagocytosis in SLE via LOX-Dependent Oxidative Stress.

Purpose: Systemic lupus erythematosus (SLE) is a severe autoimmune disease with systemic complications mediated by immune-complex formation. The elevated level of anti-phosphatidylserine (PS) IgG has been implicated in SLE pathogenesis. In this study, we aimed to explore the effector mechanisms of PS immune-complex during lupus development.

NSUN2 promotes colorectal cancer progression by stabilizing PHGDH mRNA to promote serine metabolism reprogramming.

Purpose: Cancer cells rely on serine biosynthesis for growth, but its regulation in colorectal cancer (CRC) remains not well understood. This study identifies the mC methyltransferase NSUN2 (NOP2/Sun domain family, member 2) as a key regulator of serine biosynthesis, revealing a novel mechanism driving CRC progression.

Methods: The expression and prognostic value of NSUN2 were evaluated using bioinformatics analyses and immunohistochemistry (IHC) assays.

Potential Cosmetic Applications of the Combined Extract of Panax ginseng, Ganoderma lucidum, Cordyceps militaris, and Several Asian Plants.

Objective: Although bioactive compounds from single herbs are extensively explored in cosmetics, the synergistic potential of herbal combinations remains understudied. This study aimed to evaluate the stability of a combined extract of Panax ginseng, Ganoderma lucidum, Cordyceps militaris, and several Asian plants (PGC), and its multifunctional efficacy for acne-related skin dysfunction.

Methods: PGC was analyzed using high-performance liquid chromatography (HPLC) for batch consistency and bioactive quantification.

Phyllotaxy and environmental factors influences on leaf trait dimensions in : a multidimensional approach within temperate forests.

Introduction: Light and soil nutrients are strong drivers of leaf trait variation, but the relative importance in shaping intraspecific trait variation across leaf developmental gradients remains poorly assessed. Previous studies mostly focused on single traits, while fewer have employed multidimensional trait syndromes framework to explore how plants optimize resource use and maintain physiological homeostasis.

Methods: We measured leaf economic traits (e.

Genome-wide characterization and expression analysis of the JRL gene family in response to hormones and abiotic stress in tomato ( L.).

Jacalin-related lectins (JRLs), a newly discovered subfamily of plant lectins, play an important role in plant growth and development and abiotic stress response. However, in the most important model and economic plant, the tomato, little is known about this gene family. Here, we conducted a genome-wide identification and characterization of the JRL gene family in tomato.

Overcoming E3 Ligase-Mediated Resistance: Development of Novel Hydrophobic Tagging-Based Degraders Targeting ALK Protein.

Traditional PROTACs, despite their groundbreaking role in targeted protein degradation (TPD), rely on E3 ubiquitin ligases and are vulnerable to resistance. In this study, we discovered norbornene- and bornane-based hydrophobic tags (HyTs) that efficiently degrade anaplastic lymphoma kinase (ALK). Notably, a novel hydrophobic tag, bornane was first identified.

Comprehensive analysis of smart bed comfort across varied resting conditions using quantitative measures.

Smart beds have become increasingly accepted, and are concurrently reshaping their lifestyles. Addressing the limited ability of smart beds to cater to health requirements, this study investigated smart bed comfort across diverse typical conditions. Objective body pressure distribution and participant-reported perceived comfort were recorded during typical smart bed usage.

A case of intestinal MALT lymphoma characterized by diffuse capillary hyperplasia nodules with hemorrhage.

A 52-year-old man presented to our department, complaining of recurrent episodes of hematochezia for half a month. Colonoscopy showed diffuse nodular mucosal eminences of approximately 2-3 mm in size in the colorectum and terminal ileum, presenting a "cobblestone" appearance, with visible capillary hyperplasia that are prone to rupture and bleeding upon light touch. Narrow-band imaging (NBI) showed that the hyperplastic capillaries presented "root-like" branching.

Integrating single-cell RNA-seq, bulk RNA-seq, and Mendelian randomization to elucidate the role of HLA-DPA1 expression levels and non-classical monocytes in the pathogenesis of idiopathic pulmonary arterial hypertension.

Aberrant immune and inflammatory responses are critical in idiopathic pulmonary arterial hypertension (IPAH). Thus, investigating immune-related therapeutic targets of IPAH is imperative. By integrating single-cell RNA sequencing (scRNA-seq), genome-wide association studies (GWAS), expression quantitative trait loci (eQTL), and single-cell eQTL (sc-eQTL) data, we identified a non-classical monocyte (NCM)-specific gene that was causally linked to PAH.

Within-host resistance evolution of ST15 Klebsiella pneumoniae in an ICU immunosuppressed patient under antibiotic pressure of polymyxins, ceftazidime-avibactam, and meropenem.

Objectives: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major pathogen in healthcare-associated infections, posing a severe and potentially fatal threat to critically ill patients. This study aims to investigate the evolution of antimicrobial resistance under the selective pressure from multiple antibiotics in immunocompromised patients in intensive care unit (ICU).

Methods: In this study, we report the complex dynamic evolution of resistance in ST15 CRKP in an immunocompromised critically ill patient, driven by adjustments in antibiotic regimens involving polymyxin, ceftazidime-avibactam (CZA), and meropenem.

Rescuing vascular dysfunction in dorsal pancreatic arteries prevents tacrolimus-induced glucose metabolism disorder in mice.

Long-term adverse effects of the immunosuppressant tacrolimus (Tac), such as nephrotoxicity, hepatotoxicity and diabetes, have been widely reported. Up to 33.6% of solid organ transplantation patients receiving Tac treatment develop hyperglycemia; however, the underlying mechanisms remain poorly understood.

A modified CO-related EIT parameter was used to evaluate pulmonary ventilation-perfusion ratio during prone position and inhaled nitric oxide therapy: a case report.

Introduction: Assessment of the V/Q ratio is crucial for understanding the pathophysiology of iNO therapy and prone position in ARDS patients. Recently, the concept of the absolute V/Q ratio measured by EIT has emerged. In this study, we first describe a case where a modified EIT parameter was employed to clinically monitor the absolute V/Q ratio in an ARDS patient during both prone positioning and iNO therapy.

Coronary Artery Spasm as a Rare Manifestation of Hypereosinophilic Syndrome: A Case Report.

Hypereosinophilic syndrome (HES) is a rare hematologic disorder characterized by persistently elevated eosinophil counts, leading to organ involvement. While eosinophilic myocarditis and endocarditis are common manifestations, coronary artery spasm as a presenting feature of HES is rare. We present the case of a 41-year-old female with a 4-year history of asthma and chronic sinusitis who presented with a 6-month history of dizziness, headaches, and syncope.

Intelligent Prediction Platform for Sepsis Risk Based on Real-Time Dynamic Temporal Features: Design Study.

Background: The development of sepsis in the intensive care unit (ICU) is rapid, the golden rescue time is short, and the effective way to reduce mortality is rapid diagnosis and early warning. Therefore, real-time prediction models play a key role in the clinical diagnosis and management of sepsis. However, the existing sepsis prediction models based on artificial intelligence still have limitations, such as poor real-time performance and insufficient interpretation.

Acetylcholine from tuft cells promotes M2 macrophages polarization in Hirschsprung-associated enterocolitis.

Background: Hirschsprung-associated enterocolitis (HAEC) is one of the most severe complications in patients with Hirschsprung's disease (HSCR). Previous research has indicated that acetylcholine (ACH) plays an anti-inflammatory role during inflammation by acting on the α7 nicotinic acetylcholine receptor(α7nAchR) to promote the secretion of anti-inflammatory factors. However, the specific role of ACH in HAEC remains unclear.

The combination of inactivated and subunit vaccines enhances protective efficacy against Mycoplasma synoviae.

Mycoplasma synoviae (MS) infection predominantly manifests as lower respiratory symptoms but also leads to reduced egg production and increased culling rates in laying hens, causing significant economic losses throughout the production cycle. Vaccination remains the most cost-effective strategy to protect layers from MS infection. Previous studies from our laboratory identified and screened immunogenic proteins of MS, culminating in the development of a subunit vaccine that provided robust protection with a two-dose immunization.

Molecular pharmacokinetic mechanism of JBP485 against aristolochic acid I (AAI) -induced nephrotoxicity.

Introduction: In this study, we investigated the protective effect of JBP485 against aristolochic acid I (AAI)-induced nephrotoxicity and explored the pharmacokinetic mechanisms. The effects of JBP485 on AAI-induced cytotoxicity and nephrotoxicity were evaluated and , respectively.

Methods: To ascertain the protective effect of JBP485 against AAI-induced nephrotoxicity, we measured levels of urea nitrogen (BUN), creatinine (CRE), and indoxol sulfate in blood and urine; determined kidney weight-to-body weight ratio; and performed hematoxylin and eosin (H&E) staining.

Downregulation of METTL3 enhances TRADD-mediated apoptosis in inflammatory bowel disease.

Dysregulation of RNA N-methyladenosine (mA) modification in intestinal epithelial cells (IECs) compromises intestinal homeostasis, which is critical for maintaining gastrointestinal functions, immunity, and barrier integrity in inflammatory bowel disease (IBD). Here we explored the role of mA modification, particularly through methyltransferase like 3 (METTL3), in IBD pathology and the apoptosis of intestinal stem cells (ISCs). Reduced mA RNA methylation and METTL3 expression were detected in IBD tissues, which correlated with increased ISC apoptosis and spontaneous enteritis in METTL3-deficient models; mechanistically, Mettl3 depletion increased TRADD expression in a mA-dependent manner, thereby augmenting the TNF-induced apoptosis pathway, whereas pharmacological inhibition of TRADD ameliorated the apoptotic phenotype in METTL3-deficient models and improved survival rates in the enteritis mouse model, suggesting a novel therapeutic avenue for IBD management.

SinoMAKS-protocol of a randomised controlled trial to evaluate the Chinese version of the non-pharmacological, multimodal MAKS intervention for people with mild to moderate dementia in Chinese nursing homes.

Introduction: The prevalence of dementia is increasing, both worldwide and in China. This disease is associated with numerous restrictions for those affected but also for caregivers and society. Due to the limited effectiveness of pharmacological interventions, more research on non-pharmacological interventions is needed.

Endoscopic misdiagnosis of a polypoid gastric neuroendocrine neoplasm: literature review.

A 75-year-old man presented to our department, complaining of recurrent abdominal pain for the past month. Esophagogastroduodenoscopy (EGD) revealed a single 0.6 cm polypoid lesion, classified as Yamada Type II, at the lesser curvature of the lower body of the stomach.

Rapid improvement in postpartum pulmonary hypertension associated with hereditary hemorrhagic telangiectasia: A case report and review of literature.

Background: Postpartum pulmonary arterial hypertension (PAH) complicated with hereditary hemorrhagic telangiectasia (HHT) is a rare condition. Diagnosing and treating PAH in patients with HHT can be challenging. To the best of our knowledge, no previous reports have investigated the efficacy of pulmonary vasodilators in improving hemodynamics in postpartum patients with this disease.

A Metabolic Signature Specific to the Patients with Type 2 Diabetes and its Association with the Pathogenesis of Diabetic-Foot Syndrome.

Oxidative stress and protein nonenzymatic glycation are key factors in diabetic-foot syndrome pathogenesis. Type 2 diabetes (T2DM) progression involves excessive gluconolactone (GDL) production, linked to endothelial injury and diabetic arteriosclerosis. This study explored GDL's role in diabetic-foot syndrome using high-performance liquid chromatography-tandem mass spectrometry to analyze sera from 75 T2DM patients (including 32 with diabetic-foot) and 36 healthy controls.