Development of Blocking ELISA Kit Based on Recombinant Monoclonal Antibody for the Serological Diagnosis of Peste Des Petits Ruminants Virus.

Background: Peste des petits ruminants (PPR) was a highly contagious and economically devastating disease affecting sheep and goats. The performance and cost of serological diagnostic kit remained a major challenge for PPR eradication.

Objective: The nucleocapsid (N) protein of PPR virus and its monoclonal antibody (mAb) were engineered for development of a blocking ELISA.