Asparaginase and Autophagy Inhibitors Effectively Remove Senescent Cells by Synergistically Limiting Asparagine Supply.

The accumulation of senescent cells (SNCs) contributes to tissue dysfunction and age-related diseases, creating an urgent need for effective senolytic strategies. We identified a metabolic vulnerability in SNCs characterized by marked downregulation of asparagine synthetase (ASNS), rendering them uniquely dependent on exogenous asparagine (Asn). This vulnerability was exploited through combined treatment with L-asparaginase (ASNase) and autophagy inhibitors, which synergistically deplete Asn via complementary mechanisms: ASNase degrades extracellular Asn pools, while autophagy inhibition blocks intracellular protein recycling as an alternative Asn source.

Correlation between the hemoglobin-to-hematocrit ratio and 3-month outcomes in patients with acute ischemic stroke: a secondary analysis based on a prospective cohort study.

Background: Our study dedicated to clarify the connection between the hemoglobin (HB) to hematocrit (HCT) (HB/HCT) ratio and adverse outcomes (AOs) in patients with acute ischemic stroke (AIS) within 3 months.

Methods: A total of 1,906 individuals with AIS were encompassed at a hospital in South Korea. A binary logistic regression model (BLRM) was utilized to explore the linear relationship between the HB/HCT ratio and AOs in patients with AIS.

GLUT3 enhances chemosensitivity in glioblastoma by transporting temozolomide and capecitabine.

Glioblastoma multiforme (GBM), the most aggressive brain cancer, is highly resistant to chemotherapy, which profoundly affects patient survival and prognosis. Temozolomide (TMZ), the sole first-line chemotherapeutic agent for GBM, faces substantial challenges in overcoming this resistance. Despite the belief that TMZ is well-absorbed in the small intestine and can effectively cross the blood-brain barrier due to its small molecular size, emerging evidence suggests that its uptake is not merely through passive diffusion across the lipid bilayer but is regulated by Wnt signaling.

Antioxidant capacity is a key factor in the difference in thermal tolerance between massive Porites lutea and branching Acropora formosa.

Identifying the reasons behind differences in thermal tolerance among reef-building corals is pivotal to projecting their ability to withstand increasing seawater temperatures. Antioxidant responses are common in marine organisms and are strongly linked with coral thermotolerance. However, the mechanism by which the antioxidant response affects the thermotolerance of coral holobionts remains unclear.

The U shape relationship between glucose and potassium ratio and mortality in patients with subarachnoid hemorrhage in the US population.

The prognostic value of the glucose-potassium ratio (GPR) in non-traumatic subarachnoid hemorrhage (SAH) remains undetermined. We investigated the association between the GPR at admission and all-cause mortality (ACM) in critically ill patients with SAH. We identified critically ill patients with SAH from the Medical Information Mart for Intensive Care database and stratified them into quartiles based on the GPR levels at admission.

AD16 Modulates Microglial Activation and Polarization to Mitigate Neuroinflammation in Ischemic Stroke Models Through α7nAChR-ERK-STAT3 Signaling.

Background: Neuroinflammation constitutes a critical pathological event subsequent to ischemic stroke. AD16, a novel anti-neuroinflammatory compound, has demonstrated efficacy in alleviating neuroinflammation in neonatal rats induced by ischemia-hypoxia. This study aims to elucidate the therapeutic utility and underlying mechanisms of AD16 in an adult ischemic stroke rat model.

Hospital Readmissions for All-Causes and Fluid Overload in Severely Reduced Kidney Function Not Requiring Dialysis-A Retrospective Cohort Study.

Aim: Chronic kidney disease is associated with frequent readmissions for ambulatory-sensitive conditions such as fluid overload. There is a paucity of literature to identify individuals at high risk of fluid overload or all-cause readmissions.

Methods: We performed a single-centre retrospective cohort study involving 783 patients with an estimated glomerular filtration rate of 11-30 mL/min/1.

Deep Learning-Enhanced Noninvasive Detection of Pulmonary Hypertension and Subtypes via Chest Radiographs, Validated by Catheterization.

Background: Pulmonary hypertension (PH) is a complex, life-threatening condition requiring noninvasive, accessible, and accurate diagnostic tools, particularly in resource-limited settings. Early and precise identification of PH and its subtypes is critical for effective management and timely intervention.

Research Question: Can deep learning (DL) methods applied to chest radiography (CXR) accurately detect PH and its subtype, congenital heart disease-associated pulmonary arterial hypertension (CHD-PAH)?

Study Design And Methods: A retrospective cohort study was conducted with 4,576 patients, including 2,288 patients with PH, who underwent CXR followed by right heart catheterization (RHC) or transthoracic echocardiography.

Re-Understanding Silk Aggregates as Bioactive Agents.

Bioactive agents play a pivotal role in determining the function and performance of biomedical products and devices across tissue engineering, regenerative medicine, and cosmetic applications. Silk fibroin (SF) is an extensively used structural biomaterial, yet its inherent bioactive potential remains unexplored for material design. In this study, we developed β-sheet-rich silk nanofiber aggregates (BSNFs) with controlled diameters at ∼20 nm through structural bioinspiration from native silk fibers.

The HbA1c/HDL-C ratio as a screening indicator of NAFLD in U.S. adults: a cross-sectional NHANES analysis (2017-2020).

Background: Non-alcoholic fatty liver disease (NAFLD), a metabolic liver disorder closely associated with obesity and diabetes, urgently requires early screening. This population-based study is the first to explore the relationship between glycemic control and a novel dyslipidemia composite index-the glycated hemoglobin/high-density lipoprotein cholesterol (HbA1c/HDL-C) ratio in individuals with NAFLD and liver fibrosis.

Methods: Data from 5,891 adults in the 2017-2020 National Health and Nutrition Examination Survey (NHANES) were analyzed.

Icaritin promotes brain functional rehabilitation in ischemic stroke rats by regulating astrocyte activation and polarization via GPER.

Background: Cerebral ischemic injury induces the polarization of astrocytes toward two different phenotypes, i.e., the proinflammatory A1 phenotype and the protective, anti-inflammatory A2 phenotype, affects the prognosis of cerebral ischemia.

Identification and Validation of an Explainable Prediction Model of Sepsis in Patients With Intracerebral Hemorrhage: Multicenter Retrospective Study.

Background: Sepsis is a life-threatening condition frequently observed in patients with intracerebral hemorrhage (ICH) who are critically ill. Early and accurate identification and prediction of sepsis are crucial. Machine learning (ML)-based predictive models exhibit promising sepsis prediction capabilities in emergency settings.

The non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio and its combination with obesity indicators as a predictor of all cause and cardiovascular mortality in non-diabetic individuals.

Background: The non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (NHHR) represents a novel composite lipid marker for atherosclerosis and cardiovascular disease (CVD). Nevertheless, the correlation between NHHR and mortality in the non-diabetic population remains indistinct.

Methods: This study included 20,774 non-diabetic individuals from the 1999-2018 National Health and Nutrition Examination Survey (NHANES).

Development and validation of multimodal deep learning algorithms for detecting pulmonary hypertension.

Transthoracic echocardiography (TTE), commonly used for initial screening of pulmonary hypertension (PH), often lacks sufficient accuracy. To address this gap, we developed and validated a multimodal fusion model for improved PH screening (MMF-PH). The study was registered in the ClinicalTrials.

Global burden of drowning and risk factors across 204 countries from 1990 to 2021.

Drowning is a major global public health issue with marked variations in incidence, mortality, and disability-adjusted life years (DALYs) across regions, age groups, and sexes. Although previous studies have examined global trends in drowning, few have comprehensively analyzed regional and national disparities while addressing potential reporting biases and confounding factors. Data from the Global Burden of Disease (GBD) Study 2021, focusing on drowning-related incidence, death rates, and DALYs, and examined trends from 1990 to 2021 across various regions and countries.

Hypnotic drug use and intraoperative fluid balance associated with postoperative delirium following pancreatic surgery: A retrospective, observational, single-center study.

Background: Postoperative delirium is a common complication after various types of major surgery. The aim of this study was to identify risk factors associated with delirium following pancreatic surgery.

Methods: Data from the patients who had pancreatic surgery between July 2020 and March 2021 in Ruijin Hospital affiliated with Shanghai Jiao Tong University School of Medicine were retrospectively analysed.

Fecal microbiota transplantation restores gut microbiota diversity in children with active Crohn's disease: a prospective trial.

Background: Clinical data on oral fecal microbiota transplantation (FMT), a promising therapy for Crohn's disease (CD), are limited. Herein, we determined the short-term safety and feasibility of FMT for pediatric patients with active CD.

Methods: In this open-label, parallel-group, single-center prospective trial, patients with active CD were treated with oral FMT capsules combined with partial enteral nutrition (PEN) (80%).

A comprehensive validation study on the influencing factors of cough-based COVID-19 detection through multi-center data with abundant metadata.

Objective: In recent years, COVID-19 has placed enormous burdens on healthcare systems. Currently, hundreds of thousands of new cases are reported monthly. World Health Organization is managing COVID-19 as a long-term disease, indicating that an efficient and low-cost detection method remains necessary.

Duodenal fluid analysis is an excellent differential diagnosis method of diseases with enterohepatic circulation disturbance.

Enterohepatic circulation is essential for maintaining a constant bile acid concentration. Diseases with enterohepatic circulation disturbances are usually difficult to diagnose definitively without the time-consuming and expensive genetic tests. This study analyzed and compared duodenal fluid in patients with biliary atresia (BA), familial intrahepatic cholestasis 2 (FIC2), and sodium taurocholate cotransporting polypeptide (NTCP) deficiency.

Therapeutic Effects and Mechanisms of Icaritin in Parkinson's Disease.

Parkinson's Disease (PD) is a neurodegenerative disorder of the central nervous system (CNS). Given the increasing age of the general population, PD has emerged as a significant public health and societal concern, impacting both individual well-being and socioeconomic progress. The present interventions have proven insufficient in impeding the progressive nature of PD.

The Role and Molecular Mechanism of Icaritin in the Treatment of Alzheimer's Disease.

Alzheimer's disease (AD), a degenerative disease of the central nervous system, affects approximately 70 million individuals worldwide. As the number of elderly in the population increases, the prevalence and incidence of AD are increasing annually. Although the drugs are currently used to alleviate certain cognitive symptoms, their overall therapeutic efficacy remains unclear.

Implementing the Nurse-Led Optimization of Volume and Blood Pressure - Enabling at Multi-Levels Using Technology Program for Chronic Kidney Disease: A Prospective Cohort Study.

Background: Fluid overload is a common manifestation of chronic kidney disease (CKD) and is associated with increased hospitalizations and death. However, severe symptomatic fluid overload is potentially preventable with early recognition of mild fluid overload and timely institution of appropriate pharmacotherapy and fluid restriction. We implemented and evaluated the outcomes of a nurse-led clinic that incorporated objective fluid volume assessment using body impedance analysis (BIA) into structured patient education and action plan coaching to patients with CKD and fluid overload.

Effect of metformin on the clinical outcomes of stroke in patients with diabetes: a systematic review and meta-analysis.

Objectives: Stroke is a major cause of death and disability globally, especially among diabetic patients. In this study, we aim to scrutinise the effects of metformin on the clinical outcomes of stroke in diabetic patients.

Design: This study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.