Inhalation of 4% and 67% hydrogen ameliorates oxidative stress, inflammation, apoptosis, and necroptosis in a rat model of glycerol-induced acute kidney injury.

Acute kidney injury (AKI) is the major complication of rhabdomyolysis (RM) clinically, which is usually mimicked by glycerol injection in basic research. Oxidative stress, inflammatory response and apoptosis are recognized to play important roles in development of this disease. Recently, numerous studies have reported the therapeutic effects of molecular hydrogen (H) on oxidative stress and inflammation-related diseases.

Secreted Glycosyltransferase RsIA_GT of AG-1 IA Inhibits Defense Responses in .

Anastomosis group AG-1 IA of Khün has a wide host range and threatens crop production. Various glycosyltransferases secreted by phytopathogenic fungi play an essential role in pathogenicity. Previously, we identified a glycosyltransferase RsIA_GT (AG11A_09161) as a secreted protein-encoding gene of AG-1 IA, whose expression levels increased during infection in rice.

Topographic Orientation of Scaffolds for Tissue Regeneration: Recent Advances in Biomaterial Design and Applications.

Tissue engineering to develop alternatives for the maintenance, restoration, or enhancement of injured tissues and organs is gaining more and more attention. In tissue engineering, the scaffold used is one of the most critical elements. Its characteristics are expected to mimic the native extracellular matrix and its unique topographical structures.

Microflower-like CoS@MoS heterostructure as an efficient bifunctional catalyst for overall water splitting.

The development of a distinguished and high-performance catalyst for H and O generation is a rational strategy for producing hydrogen fuel electrochemical water splitting. Herein, a flower-like CoS@MoS heterostructure with effective bifunctional activity was achieved using a one-pot approach the hydrothermal treatment of metal-coordinated species followed by pyrolysis under an N atmosphere. The heterostructures exhibited a 3D interconnected network with a large electrochemical active surface area and a junctional complex with hydrogen evolution reaction (HER) catalytic activity of MoS and oxygen evolution reaction (OER) catalytic activity of CoS, exhibiting low overpotentials of 295 and 103 mV for OER and HER at 10 mA cm current density, respectively.

Ligand-variable metal clusters charge transfer in Ce-Por-MOF/AgNWs and their application in photoelectrochemical sensing of ronidazole.

A photoelectrochemical sensing platform based on ligand-variable metal clusters charge transfer was established for the quantitative assay of ronidazole (RNZ) using Ce-porphyrin-metal-organic frameworks/silver nanowires (Ce-Por-MOFs/AgNWs). Rod-like Ce-Por-MOFs and well-dispersed sub-50 nm AgNWs were prepared using a hydrothermal method and polyol strategy, and then through simple drop coating to yield Ce-Por-MOFs/AgNWs nanocomposites. We investigated the intrinsic semiconducting properties of the composites.

Risk Assessment of Triflumezopyrim and Imidacloprid in Rice through an Evaluation of Residual Data.

Triflumezopyrim, a novel mesoionic insecticide used to control planthoppers, is a potential substitute for imidacloprid. In this study, triflumezopyrim and imidacloprid residues in rice were determined using a quick, easy, cheap, effective, rugged, and safe procedure combined with ultra-high-performance liquid chromatography-tandem mass spectrometry. The limit of quantification of both triflumezopyrim and imidacloprid was 0.

Application of Dual-Energy Spectral Computed Tomography in Bone Mineral Density Measurement: Phantom and Clinical Research.

Introduction: Early detection and treatment of osteoporosis through bone mineral density (BMD) measurement could aid in the prevention of osteoporosis-related fractures. We aimed to assess the parameter of dual-energy spectral CT (DesCT) consistency with BMD determination using quantitative computed tomography (QCT), thereby establishing a basis for further DesCT application for BMD determination.

Methods: We subjected the European spine phantom, which contains three vertebral bodies (V1, V2, and V3), to DesCT with different radiation doses.

Composition and antioxidant activity of anthocyanins from Aronia melanocarpa extracted using an ultrasonic-microwave-assisted natural deep eutectic solvent extraction method.

A time-saving, efficient, and environmentally friendly ultrasonic-microwave-assisted natural deep eutectic solvent (UMAE-NADES) extraction method was developed for the extraction of anthocyanins from Aronia melanocarpa. Eight different natural eutectic solvents were screened initially, and choline chloride-glycerol was selected as the extraction solvent. The extraction conditions were optimized using the response surface methodology, and the extraction rate of anthocyanins was higher than those achieved using the traditional ethanol method, natural deep eutectic solvent extraction method, and ultrasonic-microwave-assisted ethanol method.

Determination of trichlorfon using a molecularly imprinted electrochemiluminescence sensor on multi-walled carbon nanotubes decorated with silver nanoparticles.

Considering the limitations associated with existing methods for the detection of trace amounts of trichlorfon, this paper proposes a novel molecularly imprinted electrochemiluminescence (ECL) sensor for the detection of trichlorfon by utilizing the double enhancement effect of trichlorfon and Ag nanoparticles supported by multi-walled carbon nanotubes (MWCNTs/Ag NPs) in a luminol-HO ECL system. Here, trichlorfon was electropolymerized on the surface of the MWCNT/Ag NP-modified gold nanoelectrode with o-phenylenediamine to prepare the molecularly imprinted polymer-based sensor. After eluting the trichlorfon, imprinted holes for the identification of trichlorfon were retained on the sensor, which were used as signal switches to obtain different ECL intensities through the adsorption of different concentrations of trichlorfon.

Synthesis of 1,3,5-trisubstituted pyrazoles 1,3-dipolar cycloaddition of nitrile imines with ninhydrin-derived Morita-Baylis-Hillman carbonates.

An effective synthetic method for 1,3,5-trisubstituted pyrazoles 1,3-dipolar cycloaddition reaction has been developed. This reaction could smoothly proceed between ninhydrin-derived Morita-Baylis-Hillman carbonates and nitrilimines to provide a wide scope of differently substituted pyrazoles in high yields (up to 95%). In addition, the reaction mechanism was also proposed to explain its regioselectivity.

Retrospective validation of ultrasound characteristics at 19-24 weeks as predictive tools for selective intrauterine growth restriction in monochorionic diamniotic twin pregnancies: a diagnostic study.

Background: Seeking an optimal time point for ultrasound examination is important for the diagnosis of late selective intrauterine growth restriction (sIUGR) at birth in monochorionic diamniotic (MCDA) twin pregnancies. We aimed to assess the role of ultrasound characteristics at 19-24 weeks as predictive tools for late sIUGR at birth in MCDA twin pregnancies.

Methods: We retrospectively recruited 32 sIUGR and 56 normal patients with MCDA twin pregnancies.

Cyanidin-3-galactoside from ameliorates silica-induced pulmonary fibrosis by modulating the TGF-β/mTOR and NRF2/HO-1 pathways.

Cyanidin-3-galactoside (C3G), the most abundant anthocyanin in a, has many beneficial health effects, such as antioxidation. C3G was extracted from and applied (100, 200, and 400 mg/kg body weight) to 50-μl silica particles (SP) solution-exposed mice to research its antifibrotic properties using histological analysis, hydroxyproline assay, quantitative real-time polymerase chain reaction, and western blot analysis. The results showed that C3G treatment significantly ameliorated pulmonary fibrosis and cell infiltration into the lungs of mice.

Paraneoplastic Pemphigus Autoantibodies Against C-terminus of Desmoplakin Induced Acantholysis and .

Paraneoplastic pemphigus (PNP) is an autoimmune bullous disease associated with underlying neoplasms and characterized by antibodies against desmoglein 3 (Dsg 3) and plakins. Autoantibodies against desmoglein 3 in sera of patients with PNP have been proven to cause acantholysis in neonatal mice. As a member of the plakin family, autoantibodies against desmoplakin were detected frequently by immunoprecipitation in the sera of PNP.

A straightforward plant prime editing system enabled highly efficient precise editing of rice Waxy gene.

CRISPR Cas9-mediated genome editing is highly efficient at targeted site-specific gene knock-out through NHEJ (Non-Homology End Joining), but ineffective for specific DNA integration through HDR (Homology Directed Repair) for precise gene editing. Base editors can make limited base substitutions but only within restricted small windows of the protospacer. Prime editing has been applied in plants with various degrees of success.

Cerebral venous hemodynamic responses in a mouse model of traumatic brain injury.

Traumatic brain injury (TBI) is a serious public health problem that endangers human health and is divided into primary and secondary injuries. Previous work has confirmed that changes in cerebral blood flow (CBF) are related to the progression of secondary injury, although clinical studies have shown that CBF monitoring cannot fully and accurately evaluate disease progression. These studies have almost ignored the monitoring of venous blood flow; however, as an outflow channel of the cerebral circulation, it warrants discussion.

Optimization of Naringenin Nanoparticles to Improve the Antitussive Effects on Post-Infectious Cough.

Naringenin (NRG) is a natural compound with several biological activities; however, its bioavailability is limited owing to poor aqueous solubility. In this study, NRG nanoparticles (NPs) were prepared using the wet media milling method. To obtain NRG NPs with a small particle size and high drug-loading content, the preparation conditions, including stirring time, temperature, stirring speed, and milling media amount, were optimized.

A single-cell transcriptomic atlas tracking the neural basis of division of labour in an ant superorganism.

Ant colonies with permanent division of labour between castes and highly distinct roles of the sexes have been conceptualized to be superorganisms, but the cellular and molecular mechanisms that mediate caste/sex-specific behavioural specialization have remained obscure. Here we characterized the brain cell repertoire of queens, gynes (virgin queens), workers and males of Monomorium pharaonis by obtaining 206,367 single-nucleus transcriptomes. In contrast to Drosophila, the mushroom body Kenyon cells are abundant in ants and display a high diversity with most subtypes being enriched in worker brains, the evolutionarily derived caste.

Long non-coding RNA (LncRNA) CHROMR promotes the expression of the gene by adsorbing hsa-miR-1299 to obtain drug resistance in diffuse large B lymphoma cells.

Background: Long non-coding RNA (LncRNA) play roles in different diseases, LncRNA is differentially expressed in diffuse large B cell lines with varying degrees of resistance to rituximab.

Methods: In the GEO database (GSE159852), we found that CHROMR (cholesterol induced regulator of metabolism RNA) may be differentially expressed in different rituximab-resistant diffuse large B lymphoma cell lines. We also verified the expression level in cell lines and verified the role of CHROMR in acquiring cell drug resistance through various biological function experiments.

Silk-based hydrogel incorporated with metal-organic framework nanozymes for enhanced osteochondral regeneration.

Osteochondral defects (OCD) cannot be efficiently repaired due to the unique physical architecture and the pathological microenvironment including enhanced oxidative stress and inflammation. Conventional strategies, such as the control of implant microstructure or the introduction of growth factors, have limited functions failing to manage these complex environments. Here we developed a multifunctional silk-based hydrogel incorporated with metal-organic framework nanozymes (CuTA@SF) to provide a suitable microenvironment for enhanced OCD regeneration.

The impact of climate aid on carbon emissions reduction and the role of renewable energy: evidence from the Belt and Road countries.

Full mobilization and effective use of climate aid is of great importance for the low carbon transition of Belt and Road Initiative (BRI) countries. This study utilizes the two-step system generalized method of moments model to evaluate the effects of climate aid on carbon emissions reduction and the impact mechanism through energy structure optimization. The panel data of 93 Belt and Road countries from 2000 to 2018 were used for empirical analysis.

An Antigen: Its Functions on Stimulating Th1 Cytokines and Protective Efficacy Against Coccidiosis.

A consensus is that the Th1 immune response plays a predominant role against avian coccidiosis. Therefore, an antigen with the ability to induce Th1 cytokine responses is an ideal candidate for the development of coccidiosis vaccines. In our previous study, EmARM-β, a Th1 cytokines-stimulating antigen, was screened from the cDNA expression library of ().

Effects of Zinc, Magnesium, and Iron Ions on Bone Tissue Engineering.

Large-sized bone defects are a great challenge in clinics and considerably impair the quality of patients' daily life. Tissue engineering strategies using cells, scaffolds, and bioactive molecules to regulate the microenvironment in bone regeneration is a promising approach. Zinc, magnesium, and iron ions are natural elements in bone tissue and participate in many physiological processes of bone metabolism and therefore have great potential for bone tissue engineering and regeneration.