Antifungal effect and control of Alternaria fruit rot in blueberry through ε-polylysine hydrochloride.

Blueberry was easily infected by Alternaria sp. during storage time, leading to Alternaria fruit rot (AFR). ε-polylysine hydrochloride (ε-PL), a widely used food additive, was evaluated for its antifungal efficacy at different concentrations and its effects on the quality of blueberries treated with 3 g L ε-PL.

Effects of mannoprotein on the stability and in vitro digestion of cyanidin-3-glucoside.

Although mannoprotein (MP) is known to increase the stability of anthocyanins, the MP-anthocyanin interactions and structural changes induced by the same remain underexplored. To bridge this gap, this work examined the complexation of cyanidin-3-glucoside (C3G) by MP and probed its effect on C3G properties. As a result, this complexation was shown to induce the static fluorescence quenching of MP and increase the thermal stability and antioxidant activity of C3G while decreasing its susceptibility to ascorbic acid, sucrose, and Fe-induced degradation and increasing its bioavailability during simulated in vitro digestion.

Proteomic analyses revealed the antibacterial mechanism of isolated anthocyanins against O157: H7.

anthocyanins (AMAs), as a natural plant extract, can control pathogens and are attracting increasing attention. However, at the proteomic level, the antibacterial mechanism of AMAs against O157: H7 remains unclear. In this study, the tandem mass tag (TMT) quantitative proteomics was used to elucidate the potential antibacterial mechanisms of AMAs against O157: H7 cells.

Composition and antioxidant activity of anthocyanins from Aronia melanocarpa extracted using an ultrasonic-microwave-assisted natural deep eutectic solvent extraction method.

A time-saving, efficient, and environmentally friendly ultrasonic-microwave-assisted natural deep eutectic solvent (UMAE-NADES) extraction method was developed for the extraction of anthocyanins from Aronia melanocarpa. Eight different natural eutectic solvents were screened initially, and choline chloride-glycerol was selected as the extraction solvent. The extraction conditions were optimized using the response surface methodology, and the extraction rate of anthocyanins was higher than those achieved using the traditional ethanol method, natural deep eutectic solvent extraction method, and ultrasonic-microwave-assisted ethanol method.

Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.

The purpose of this study was to screen the method with the highest yield of mannoprotein (MP) without damaging their functional structure. The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods. The MP extracts recovery rate prepared by the thermal method was significantly higher (35.