Long-Read Sequencing Reveals Tumor-Specific Splicing Isoforms as Therapeutic Targets In NSCLC.

Despite extensive transcriptomic alterations observed in tumors, the global landscape of isoform-level alternative splicing in cancer remains largely unexplored. Leveraging long-read sequencing, we successfully identified and characterized full-length isoforms, along with tumor-specific splicing events in non-small cell lung cancer (NSCLC). Our analysis identified 38,058 previously unannotated isoforms, which were subsequently validated using orthogonal multi-omics datasets to confirm their transcriptional and translational activities.

Dynamic molecular landscape in dorsal root ganglion for peripheral nerve regeneration promoted by tissue engineered nerve graft.

Introduction: The limited regenerative capacity of the nervous system represents a significant clinical challenge in the context of peripheral nerve injuries. An innovative strategy for sciatic nerve repair has been developed using tissue-engineered nerve grafts (TENGs) composed of skin-derived precursor Schwann-like cells (SKP-SCs) and a silk fibroin-chitosan scaffold. However, the reason why SKP-SCs-TENG demonstrated superior enhanced nerve regeneration compared to the autograft and scaffold groups remains unclear.

The protective role of vitamin B1 in alcohol related headaches induced by disturbance of blood flow and direct cytotoxicity to hippocampal neuron.

Objectives: To evaluate vitamin B1 in alcohol-induced headaches and elucidate its underlying mechanisms and potential preventive applications.

Methods: In vitro cellular assays assessed the cytotoxicity of alcohol and the protective effects of vitamin B1 on components of the mouse neurovascular units, including endothelial cells, astrocytes, and neurons. Murine models were used to study the effects of alcohol on cerebral blood flow and neurons, as well as the neuroprotective efficacy of vitamin B1.

Dual production of biconvex polymer particles via surfactant-laden microfluidic ternary droplets.

Lens-shaped particles with uniform size and precisely controlled shape are essential for applications ranging from controlled self-assembly to advanced micro-optical elements. We present a microfluidic method for generating surfactant-laden ternary droplets that enables the simultaneous fabrication of two biconvex polymeric particles with independently controlled sizes and shapes. This approach uses two photocurable acrylate monomer streams, separated by a non-curable silicone oil stream containing a surfactant, which are emulsified into nanoliter-sized ternary droplets within an aqueous polyvinyl alcohol solution.

Optical imaging of flavor order in flat band graphene.

Spin- and valley flavor polarization plays a central role in the many-body physics of flat band graphene, with Fermi surface reconstruction - often accompanied by quantized anomalous Hall and superconducting state - observed in a variety of experimental systems. Here we describe an optical technique that sensitively and selectively detects flavor textures via the exciton response of a proximal transition metal dichalcogenide layer. Through a systematic study of rhombohedral and rotationally faulted graphene bilayers and trilayers, we show that when the semiconducting dichalcogenide is in direct contact with the graphene, the exciton response is most sensitive to the large momentum rearrangement of the Fermi surface, providing information that is distinct from and complementary to electrical compressibility measurements.

[Application of multi-scale spatiotemporal networks in physiological signal and facial action unit measurement].

Multi-task learning (MTL) has demonstrated significant advantages in the field of physiological signal measurement. This approach enhances the model's generalization ability by sharing parameters and features between similar tasks, even in data-scarce environments. However, traditional multi-task physiological signal measurement methods face challenges such as feature conflicts between tasks, task imbalance, and excessive model complexity, which limit their application in complex environments.

Celastrol Induces Ferroptosis by Regulating CERKL to Exert Anti-Gastric Cancer Effect.

Gastric cancer is a significant global health issue. Celastrol, a natural compound, has shown antitumor potential, but its molecular mechanism in gastric cancer remains unclear. In this study, we treated HGC-27 cells with celastrol and employed CCK8, colony formation, and Transwell assays, revealing its inhibitory effect on cell proliferation and migration.

Superconductivity and spin canting in spin-orbit-coupled trilayer graphene.

Graphene and transition metal dichalcogenide flat-band systems show similar phase diagrams, replete with magnetic and superconducting phases. An abiding question has been whether magnetic ordering competes with superconductivity or facilitates pairing. For example, recent studies of Bernal bilayer graphene in the presence of enhanced spin-orbit coupling show a substantial increase in the observed domain and critical temperature T of superconducting states; however, the mechanism for this enhancement remains unknown.

Two new species of larval Erythraeidae (Parasitengona) ectoparasites of leafhoppers from Southwestern China.

In this study, three species were examined. Among them, two new species, and from Southwestern China, were described and illustrated based on larvae. The two new species can be distinguished from the known species by the following characteristics: the anterior sensilla are nude, and the gnathosoma has two pairs of nude hypostomalae in ; the anterior sensilla and posterolateral scutalae are located in the posterior half of the scutum, and the posterior hypostomalae with barbs on the proximal half in In addition, a new host and distribution range of Xu & Jin, 2022 is reported.

SWI/SNF complex-mediated ZNF410 cooperative binding maintains chromatin accessibility and enhancer activity.

The clustering of multiple transcription factor binding sites (TFBSs) for the same TF has proved to be a pervasive feature of cis-regulatory elements in the eukaryotic genome. However, the contribution of binding sites within the homotypic clusters of TFBSs (HCTs) to TF binding and target gene expression remains to be understood. Here, we characterize the CHD4 enhancers that harbor unique functional ZNF410 HCTs genome wide.

Fluctuating magnetism and Pomeranchuk effect in multilayer graphene.

Magnetism typically arises from the effect of exchange interactions on highly localized fermionic wavefunctions in f- and d-atomic orbitals. By contrast, in rhombohedral multilayer graphene (RMG), magnetism-manifesting as spontaneous polarization into one or more spin and valley flavours-originates from itinerant electrons near a Van Hove singularity. Here we show experimentally that the electronic entropy in this system indicates signatures typically associated with disordered local magnetic moments, unexpected for electrons in a fully itinerant metal.

Robotic-based Experimental Procedure for Colorimetric Gas Sensing Development.

This paper presents a robot-based experimental program aimed at developing an efficient and fast colorimetric gas sensor. The program employs an automated Design-Build-Test-learning (DBTL) approach, which optimizes the search process iteratively while optimizing multiple recipes for different concentration intervals of the gas. In each iteration, the algorithm generates a batch of recipe suggestions based on various acquisition functions, and with the increase in the number of iterations, the values of weighted objective function for each concentration interval significantly improve.

LoreX: A Low-Energy Region Explorer Boosts Efficient Crystal Structure Prediction.

Machine learning has boosted the remarkable development of crystal structure prediction (CSP), greatly accelerating modern materials design. However, slow location of the low-energy regions on the potential energy surface (PES) is still a key bottleneck for the overall search efficiency. Here, we develop a low-energy region explorer (LoreX) to rapidly locate low-energy regions on the PES.

Imbalance of bladder neurohomeostasis by Myosin 5a aggravates diabetic cystopathy.

Background: Diabetic cystopathy (DCP) is linked to bladder nerve conduction disorders, with diabetes-induced neuropathy impairing nerve signal transmission and causing bladder dysfunction. Myosin 5a, vital for neuronal transport, has been linked to neurological disorders, though its role in DCP remains unclear. The objective of this study was to investigate whether Myosin 5a plays a potential regulatory role in Diabetic Cystopathy.

Astragalus Polysaccharides Ameliorate Diabetic Bladder Dysfunction via Normalization of Neuromuscular Conduction.

Neuromuscular conduction dysfunction-induced underactive bladder (UAB) is a major urological complication associated with diabetes mellitus (DM), and there remain deficiencies in reliable pharmacological treatment options. Astragalus polysaccharides (APS), as an edible active substance in , have a therapeutic effect on diabetes and its complications. We investigated the effects and mechanism of APS in high-fat-diet-induced (HFD) diabetic UAB mice.

Suoquan Wan mitigates bladder overactivity via modulation of neuroimmune homeostasis in perimenopausal rats.

Background: Overactive bladder (OAB) is a common disorder in perimenopausal women, involving detrusor regulation by bladder neuro-immune interactions. While Suoquan Wan (SQW) has shown efficacy in alleviating OAB symptoms, its underlying mechanisms remain unclear.

Purpose: The aim of this study was to investigate the therapeutic effects of SQW on perimenopausal overactive bladder (OAB) and elucidate its underlying mechanisms in restoring bladder neuro-immune homeostasis.

Triptolide's impact on ACER1 signaling: Inducing autophagy for triple-negative breast cancer suppression.

Given the absence of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (Her-2) in triple-negative breast cancer (TNBC) cells, the efficacy of targeted therapies is limited. In this study, we uncovered that triptolide (TP) effectively suppresses the migration and invasiveness of MDA-MB-231 cells by activating autophagic pathways. Western blotting analysis revealed that TP significantly reduced the expression levels of p62 protein, while simultaneously markedly increasing the expression levels of LC3B-II, BNIP3, BNIP3L, ATG5, and ULK1 proteins, strongly suggesting an enhancement of autophagic activity in the cells.

Genome-wide analysis of Class III peroxidase (PRX) family core genes and functional mechanism of GhPRXR1-A for seed development in Gossypium hirsutum.

Class III peroxidases (PRXs) play critical roles in plant growth and development by oxidizing various substrates with HO. Although many PRXs have been identified and their roles in biotic and abiotic stress responses have extensively investigated in plants. However, functional mechanisms of PRXs in seed development remain poorly understood.

Unveiling the molecular blueprint of SKP-SCs-mediated tissue engineering-enhanced neuroregeneration.

Peripheral nerve injury poses a significant challenge to the nervous system's regenerative capacity. We previously described a novel approach to construct a chitosan/silk fibroin nerve graft with skin-derived precursor-induced Schwann cells (SKP-SCs). This graft has been shown to promote sciatic nerve regeneration and functional restoration to a level comparable to that achieved by autologous nerve grafts, as evidenced by behavioral, histological, and electrophysiological assessments.

Four new species of Gallerucida Motschulsky, 1861 from China (Coleoptera: Chrysomelidae: Galerucinae).

Four new species of the genus Gallerucida Motschulsky, 1861 from China, G. mantillerii sp. nov.

Non-Contact Cross-Person Activity Recognition by Deep Metric Ensemble Learning.

In elderly monitoring or indoor intrusion detection, the recognition of human activity is a key task. Owing to several strengths of Wi-Fi-based devices, including their non-contact and privacy protection, these devices have been widely applied in the area of smart homes. By the deep learning technique, numerous Wi-Fi-based activity recognition methods can realize satisfied recognitions, however, these methods may fail to recognize the activities of an unknown person without the learning process.

Establishing a Multifaceted Comprehensive Maternity Cohort Facilitates Understanding of How Environmental Exposures Impact Perinatal Health.

China's "three-child policy", implemented in response to population aging, has made the protection of maternal and infant health an urgent priority. In this environmental and medical big-data era, the Zhejiang Environmental and Birth Health Research Alliance (ZEBRA) maternity cohort was established with the aim of identifying risk factors for perinatal morbidity and mortality from the perspectives of both observational epidemiology and experimental etiology. Compared with conventional birth cohorts, the inclusion of a maternity cohort allows greater scope for research and places an emphasis on maternal health.