Eco-engineered recombinant collagen III-PEG hydrogels with curcumin synergy for accelerated skin regeneration: A green crosslinking strategy in wound therapeutics.

The development of immunologically safe collagen-based hydrogels remains challenging due to risks associated with animal-derived collagens and toxic crosslinkers. Here, we report an eco-friendly hydrogel platform integrating recombinant humanized collagen type III (PCIII) expressed in Pichia pastoris with 4-arm PEG-SS via a one-step green crosslinking strategy. The engineered PEG-PCIII hydrogels exhibit tunable mechanics, enzymatic degradability, and rapid self-gelation through amine-NHS ester conjugation, while curcumin loading endows it with adjustable antioxidant activity.

FARSB downregulation by SPI1 modulates lung adenocarcinoma progression and immune microenvironment via mTOR pathway.

Background: Phenylalanyl-TRNA Synthetase Subunit Beta (FARSB) is implicated in the progression of multiple cancers and represents a potential therapeutic target. However, its role in lung adenocarcinoma (LUAD) progression and the immune microenvironment remains poorly understood, warranting further investigation into its regulatory mechanisms.

Methods: We conducted bioinformatics analyses to investigate the expression levels of FARSB in LUAD, identify enriched pathways, and assess its correlation with patient prognosis and CD8+ T cell infiltration.

Peptide codes for organ-selective mRNA delivery.

Organ-selective delivery of messenger RNA (mRNA) is critical for fulfilling the therapeutic potential of mRNA-based gene and protein replacement technologies. Despite clinical advances in the hepatic delivery of mRNA using lipid nanoparticles (LNPs), current strategies for extrahepatic-organ-selective mRNA delivery still have limitations. Here we report a peptide-encoded organ-selective targeting (POST) method for the delivery of mRNA to extrahepatic organs after systemic administration, which is based on the modular tuning of LNPs through surface engineering with specific amino acid sequences (POST codes).

Mapping a New Blueprint of Multimodal Immunotherapy Against Prostate Cancer: Breakthroughs, Barriers, and Bold Frontiers.

Immunotherapies have achieved remarkable success in treating various malignant tumors in recent years; however, their application in prostate cancer remains in the early stages of development. This is mainly because the disease has low immunogenicity, and its complex immune microenvironment limits the efficacy of single immunotherapy. Researchers are increasingly exploring novel immunotherapy strategies for this disease.

Impact of high-speed rail opening on the site selection of renewable energy enterprises: empirical evidence from China.

High-speed rail is a crucial transportation infrastructure in China and has a significant impact on the location choice of renewable energy enterprises. However, it is unclear how substantial is this impact, and how can it be measured. Research on these topics is quite limited.

Psoralen alleviates ulcerative colitis by suppressing inflammation, modulating oxidative stress, and regulating ferroptosis.

Objective: This study aimed to systematically evaluate the therapeutic effects of Psoralen (PSO) on ulcerative colitis (UC) and investigate its underlying mechanisms, with a particular focus on intestinal barrier function, gut microbiota, inflammation, oxidative stress, and ferroptosis.

Methods: UC was induced in mice via 2.25 % dextran sulfate sodium (DSS).

Molecularly imprinted photoelectrochemical sensor based on Z-scheme SnInS/CdS heterojunction for ultrasensitive detection of colchicine.

Herein, a Z-scheme SnInS/CdS heterojunction with excellent photoelectric conversion efficiency as a photoelectric signal probe was prepared to construct a molecularly imprinted photoelectrochemical sensor (MIP-PECS) for ultrasensitive detection of colchicine (Col). The Z-scheme heterojunction exhibited remarkable photostability and photocurrent signals due to the synergistic optimization of broad-spectrum light absorption and high charge separation efficiency. Moreover, the molecularly imprinted polymer (MIP) film was formed by polymerization of the functional monomer m-phenylenediamine (m-PD), following by creating a tailored three-dimensional imprinted cavity with Col recognition sites.

Cystatin F-a key player in central nervous system disease.

Cystatin F is an endogenous cysteine protease inhibitor that belongs to the type II cystatin family. It has several unique characteristic structures that determine some of its specific functions. Cystatin F is expressed predominantly in peripheral immune cells and in the microglia of the central nervous system (CNS).

Sheehan's syndrome complicated with Graves' disease in a woman with a 20-year history of hypothyroidism.

Sheehan's syndrome is a disorder caused by ischemic necrosis of the pituitary gland following postpartum hemorrhage, typically resulting in pituitary dysfunction that can affect the adrenal, thyroid, and gonadal axes. Clinically, patients may present with dysfunction of a single axis or multiple axes. The co-occurrence of Sheehan's syndrome and Graves' disease is extremely rare, posing a significant diagnostic challenge.

Nanog overexpression enhances the therapeutic efficacy of ADMSCs in AMI rats via the upregulation of JAK/STAT3 signaling and cyclin-mitochondrial expression.

This study investigated whether Nanog-overexpressing adipose-derived mesenchymal stem cells (Nanog-ADMSCs) are superior to unmodified ADMSCs in improving the left ventricular ejection fraction (LEVF) in acute myocardial infarction (AMI) patients. We utilized silencing and overexpression of Nanog gene in ADMSCs and performed a wound healing assay/transwell migration assay/MTT cell viability assay/left coronary artery ligation for AMI induction. Additionally, we categorized the cells into three classes [i.

Dynamic molecular landscape in dorsal root ganglion for peripheral nerve regeneration promoted by tissue engineered nerve graft.

Introduction: The limited regenerative capacity of the nervous system represents a significant clinical challenge in the context of peripheral nerve injuries. An innovative strategy for sciatic nerve repair has been developed using tissue-engineered nerve grafts (TENGs) composed of skin-derived precursor Schwann-like cells (SKP-SCs) and a silk fibroin-chitosan scaffold. However, the reason why SKP-SCs-TENG demonstrated superior enhanced nerve regeneration compared to the autograft and scaffold groups remains unclear.

Faces Blind Your Eyes: Unveiling the Content-Irrelevant Synthetic Artifacts for Deepfake Detection.

Data synthesis methods have shown promising results in general deepfake detection tasks. This is attributed to the inherent blending process in deepfake creation, which leaves behind distinct synthetic artifacts. However, the existence of content-irrelevant artifacts has not been explicitly explored in the deepfake synthesis.

Indigo naturalis for inflammatory bowel disease: evidence from animal studies and molecular mechanisms.

Background: Indigo naturalis (IN) has been extensively used in prescriptions of traditional Chinese medicine to treat inflammatory bowel disease (IBD), particularly ulcerative colitis (UC). However, there is a lack of quantitative, evidence-based assessments from preclinical trials.

Aims: Quantitative statistical evidence regarding the efficacy of IN in animal models of IBD remains insufficient.

Identification and validation of integrated stress-response-related genes as biomarkers for age-related macular degeneration.

Background: Age-related macular degeneration (AMD) is a prevalent ocular condition associated with aging, serving as a significant contributor to vision loss among middle-aged and older individuals. Studies have shown that AMD and integrated stress response (ISR) are associated with oxidative stress, but no specific molecular mechanisms have been identified. Therefore, this study aimed to identify potential biomarkers for AMD through bioinformatics analysis based on the transcriptome database and integrated stress response related genes (ISR-RGs).

Systematic Summary of Best Evidence for Preventing Delayed Recovery After General Anesthesia in Older Patients.

Purpose: To summarize the best evidence for preventing delayed recovery (DR) after general anesthesia in older patients.

Design: Summary of best evidence.

Methods: A comprehensive literature search was conducted in both Chinese and international databases to identify evidence on preventing DR after general anesthesia in older patients.

Evaluation of Pupal Parasitoids and as Potential Biological Control Agents of .

Parasitoid wasps are vital for biological control, and while new species continue to be discovered, evaluating their biological characteristics is crucial for realizing their potential for pest management. (Rondani) (Hymenoptera: Pteromalidae) is a well-studied parasitoid of dipteran pests, while Sureshan & Narendran (Hymenoptera: Pteromalidae) has been only morphologically described. To assess its biocontrol potential, we compared the biological traits of and using Meigen (Diptera: Drosophilidae) and (Hendel) (Diptera: Tephritidae) as hosts.

Severe ARDS Complicated by Active Pulmonary Tuberculosis and Recurrent Nosocomial Infections: Therapeutic Challenges and Clinical Outcomes.

Acute respiratory distress syndrome (ARDS) secondary to tuberculosis (TB) is rare and associated with high mortality. Management is further complicated by comorbidities and ICU-related complications. We report a 43-year-old woman with post-polio sequelae and uncontrolled diabetes who developed ARDS due to pulmonary TB, complicated by recurrent nosocomial infections and gastrointestinal bleeding.

Probiotic E10 Ameliorates Dextran Sulfate Sodium-Induced Colitis by Enhancing the Intestinal Barrier and Modulating Microbiota.

Inflammatory bowel disease (IBD) is a chronic gastrointestinal disorder associated with gut microbiota dysbiosis and impaired intestinal barrier function. Probiotic interventions have shown potential in alleviating intestinal inflammation and restoring microbial balance. This study explores the protective effects of () E10 in mice.

Sentinel-Site-Based Surveillance of Drug Resistance and Epidemiology in Sichuan, China.

Objectives: To investigate epidemiological/drug-resistance characteristics and identify potential factors related to drug-resistant and clustered tuberculosis in Sichuan.

Methods: A total of 295 (MTB) isolates were collected from surveillance sites in Sichuan from 2019 to 2021. The minimum inhibitory concentrations (MICs) of 12 anti-TB drugs were acquired using the broth microdilution method, followed by whole-genome sequencing (WGS) analysis.

Back to Work, Running on Empty? How Recovery Needs and Perceived Organizational Support Shape Employees' Vigor Upon Return to Work.

Returning to work after extended holidays poses significant challenges to employees' psychological adjustment, yet this phenomenon remains underexplored in organizational research. Drawing on the Conservation of Resources (COR) theory, this study develops and tests a moderated mediation model to examine how pre-holiday work-related irritation influences post-holiday workplace vigor through heightened need for recovery, and how perceived organizational support buffers this process. Data were collected through a four-wave time-lagged design surrounding the Chinese Spring Festival, with a final sample of 349 employees across diverse industries.

The ribonucleoprotein hnRNP K promotes hepatic steatosis by suppressing the nuclear hormone receptor PPARα.

Ectopic triglyceride accumulation resulting from impaired fatty acid β-oxidation (FAO) plays a crucial role in metabolic dysfunction-associated steatotic liver disease. The PPARα activation can promote FAO, thus reducing hepatic lipid levels. HnRNP K is known to act as an enhancer or repressor to regulate gene transcription, yet the relevance of hnRNP K to lipid metabolism remains elusive.

Gentiopicroside improves DSS-induced ulcerative colitis and secondary liver injury in mice by enhancing the intestinal barrier and regulating the gut microbiome.

Ulcerative colitis (UC) is a chronic inflammatory bowel disease frequently accompanied by intestinal barrier dysfunction and gut microbiome dysbiosis. Emerging evidence suggests that these impairments can contribute to secondary liver injury (SLI) by disrupting the gut-liver axis and promoting hepatic inflammation. Gentiopicroside (GPS), a natural iridoid glycoside, possesses antimicrobial, anti-inflammatory, and hepatoprotective properties.

Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis.

Peritoneal fibrosis (PF) is a major complication of long-term peritoneal dialysis, leading to ultrafiltration failure and technique dropout, highlighting the urgent need for therapies that can preserve peritoneal membrane function and longevity. The present study evaluated the effectiveness of dulaglutide in preserving the functional integrity and durability of the peritoneum while inhibiting PF. Met-5A cells showed significant upregulation of inflammatory, oxidative stress, intracellular and mitochondrial reactive oxygen species (ROS), fibrotic, intracellular cytoskeletal, apoptotic and epithelial-mesenchymal transition (EMT) biomarkers, and dipeptidyl peptidase 4 (DPP4), following stimulation with a uremic toxin (p-Cresol), PF inducer [chlorhexidine gluconate (CG)] or endotoxin [lipopolysaccharide (LPS)].

COVID-19 Surveillance in Madagascar and Urban Burkina Faso: Addressing Underreporting of Disease Burden Through Integrative Analysis of Diverse Data Streams.

Background: Coronavirus disease 2019 (COVID-19) caused substantial disease and death worldwide since December 2019, but the burden was lower in Africa than in high-income countries. To address potential underreporting, we modeled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and disease burden in Burkina Faso and Madagascar.

Methods: Prospectively enrolled patients who presented with fever at sentinel healthcare facilities were assessed for active SARS-CoV-2 infection.

The Intervention Effect of Digital Health Technology on Anxiety, Depression, and Treatment Adherence in Maintenance Hemodialysis Patients: A Meta-Analysis.

A systematic assessment was conducted to evaluate the effectiveness of digital health technology interventions on anxiety, depression, and treatment adherence in maintenance hemodialysis patients. A computerized search of 10 databases of randomized controlled trials of digital health technologies on anxiety, depression, and treatment adherence in maintenance hemodialysis patients was conducted for the period from database construction to December 5, 2024, and a meta-analysis of the included literature was performed using RevMan 5.4 and Stata 15.