The ribonucleoprotein hnRNP K promotes hepatic steatosis by suppressing the nuclear hormone receptor PPARα.

Ectopic triglyceride accumulation resulting from impaired fatty acid β-oxidation (FAO) plays a crucial role in metabolic dysfunction-associated steatotic liver disease. The PPARα activation can promote FAO, thus reducing hepatic lipid levels. HnRNP K is known to act as an enhancer or repressor to regulate gene transcription, yet the relevance of hnRNP K to lipid metabolism remains elusive.

Lattice Boltzmann simulation for phase separation with chemical reaction controlled by ultrasound field.

In this work, the phase separation behavior and pattern formation in binary fluids with chemical reactions controlled by ultrasonic radiation were systematically investigated. We incorporated the density-dependent Arrhenius equation into a novel and modified model for phase separation. The coupling effects of the pre-exponential factor K, density, and frequency on the phase separation under the condition of ultrasonic field-regulated chemical reactions were evaluated.

Metal Armor-Decorated Neutrophil Micromotors with Self-Propelled and Targeting Function for Brain Injury Precise Therapy.

Radiation-induced brain injury (RIBI) is a common brain injury following radiotherapy to the head and neck region, which is often accompanied by severe cognitive dysfunction, seriously affecting the quality of life of patients. Studies have established that excessive free radicals produced by radiation are mainly responsible for RIBI. However, there are currently no clinically effective drugs for RIBI treatment.

Self-Propulsion of Biomimetic Nanomotors Promotes Diffusion and Convection Transport for Enhanced Radiotherapy in Solid Glioblastoma.

As a principal adjuvant therapy for glioblastoma (GBM), radiotherapy (RT) is instrumental in extending patient survival. Radiosensitizers could enhance the cytotoxic effects of radiation on tumor cells. However, their accumulation and penetration are significantly hindered by the blood-brain barrier (BBB) and weak convection and diffusion due to elevated interstitial pressure and the dense extracellular matrix within GBM, which reduces the effectiveness of RT.

Pan-Genome-Wide Association Study Identifies Genetic Factors Associated with the Pathogenicity of Invasive Serotype 19F Streptococcus Pneumoniae.

Background: is a common respiratory pathogen that poses significant health concerns in children, particularly serotype 19F strains that demonstrate high level of invasiveness in China. To investigate the genetic variations associated with high invasiveness of serotype 19F strains isolated from children in Shenzhen.

Methods: We compared the genomic profiles of 42 invasive and 162 noninvasive strains from children's respiratory tracts and employed pan-genome-wide association methods to elucidate the origins of genetic variation.

EPHA5 regulates antifungal innate immunity by phosphorylating EPHB2 and Dectin-1.

Invasive fungal infections (IFIs) have emerged as a significant health threat and cause approximately 3.75 million deaths per year globally. Understanding the detailed mechanism of the immune response to eliminate invasive fungal pathogens may help to provide new insights for the development of antifungal methods and drugs.

The role of IL-18 and IL-33 in the bronchoalveolar lavage fluid of children with severe community-acquired pneumonia complicated with pleural effusion.

Background: To investigate the evaluative role of interleukin (IL)-1 family cytokines in bronchoalveolar lavage fluid (BALF) among children with severe community-acquired pneumonia (SCAP) and identify cytokines with clinical relevance for pediatric SCAP.

Methods: Children with SCAP hospitalized at Shenzhen Children's Hospital (2019-2020) were studied. IL-1 family cytokines in the BALF were measured via CBA or ELISA.

Epidemiological Shifts in Children Respiratory Pathogens in Shenzhen, China: A Comparative Analysis Before and After the Relaxation of COVID-19 Non-Pharmaceutical Interventions.

Background: The COVID-19 pandemic and associated non-pharmaceutical interventions significantly altered the epidemiology of respiratory pathogens. This study aimed to evaluate the changes in the prevalence and distribution of respiratory pathogens among children with acute respiratory infections (ARIs) before and after the relaxation of COVID-19 restrictions in Shenzhen, China.

Methods: This study enrolled hospitalized children with ARIs in Shenzhen Children's Hospital during the COVID-19 epidemic and those post-epidemic period.

Massage-Mimicking Nanosheets Mechanically Reorganize Inter-organelle Contacts to Restore Mitochondrial Functions in Parkinson's Disease.

Parkinson's disease (PD) is exacerbated by dysfunction of inter-organelle contact, which depends on cellular responses to the mechanical microenvironment and can be regulated by external mechanical forces. Delivering dynamic mechanical forces to neural cells proves challenging due to the skull. Inspired by the effects of massage; here PEGylated black phosphorus nanosheets (PEG-BPNS), known for their excellent biocompatibility, biodegradability, specific surface area, mechanical strength, and flexibility, are introduced, which are capable of adhering to neural cell membrane and generating mechanical stimulation with their lateral size of 200 nm, exhibiting therapeutic potential in a 1-methyl-4-phenyl-1,2,3,6-te-trahydropyridine-induced PD mouse model by regulating inter-organelle contacts.

Clinical characteristics of severe community-acquired pneumonia in children with virus mono-detection versus co-detection with bacteria.

Backgroud: By analyzing the etiological distribution and clinical characteristics of severe community-acquired pneumonia in children with virus mono-detection and co-detection with bacteria and other pathogens, to explore the clinical characteristics that can help identify mixed infections, thereby providing a basis for the more precise use of antimicrobial drugs.

Methods: A retrospective study was conducted on hospitalized children aged 1 month to 14 years with severe community-acquired pneumonia who underwent bronchoscopy in Shenzhen Children's Hospital from January to December 2018. The distribution of 19 pathogens detected by nucleic acid detection in bronchoalveolar lavage fluid was analyzed.

Modeling of lung organoid-based fibrosis for testing the sensitivity of anti-fibrotic drugs.

Background: Pulmonary fibrosis models play crucial roles in research of pulmonary fibrosis and anti-fibrosis drug screening. Despite the establishment of several pulmonary fibrosis models including lung fibrosis animals, stem cell differentiation, pulmospheres and so on, the one that mimic the personalized native lung lacks.

Methods: We here developed a lung organoid-based fibrosis (LOF) model from native lung tissue, and the potential of the LOFs for the sensitivity test of anti-fibrotic drugs was evaluated.

RTGN: Robust Traditional Chinese Medicine Graph Networks for Patient Similarity Learning.

Traditional Chinese Medicine (TCM) boasts a long history and a unique diagnostic and therapeutic paradigm. Integrating TCM with Western medicine and modern medical devices has yielded numerous successful cases in recent years. TCM treatment has developed a special knowledge framework focusing on precise differentiation based on multidimensional information such as the patient's diseases, symptoms, and syndromes.

Oral iron sulfide prevents acute alcohol intoxication by initiating the endogenous multienzymatic antioxidant defense system.

Acute alcohol intoxication could cause multiorgan damage, including nervous, digestive, and cardiovascular systems, and in particular, irreversible damage to the brain and liver. Emerging studies have revealed that the endogenous multienzymatic antioxidant defense system (MEAODS) plays a central role in preventing oxidative stress and other toxicological compounds produced by alcohol. However, few available drugs could quickly regulate MEAODS.

Correction of severe varus deformity in total knee arthroplasty with the technique of tibial plateau reduction.

Background: The optimal soft tissue release technique for severe varus total knee arthroplasty (TKA) remains controversial. The technique of tibial plateau reduction was suggested for severe varus deformity during TKA. The purpose of this study was to evaluate the clinical and radiological outcomes of the technique of tibial plateau reduction.

Nanoenzymes-Integrated and Microenvironment Self-Adaptive Hydrogel for the Healing of Burn Injury and Post-Burn Depression.

Burn injuries often cause prolonged oxidative stress and inflammatory pain due to an initial increase in inflammatory responses, consequently exacerbating depressive disorders and severely impairing patients' quality of life. The primary function of traditional burn dressings is to prevent infection and facilitate tissue repair. However, these dressings are not intended for the inflammatory pain and depression that often occur during recovery.

MYO1F positions cGAS on the plasma membrane to ensure full and functional signaling.

Cyclic guanosine monophosphate (GMP)-adenosine monophosphate (AMP) synthase (cGAS) detects viral or endogenous DNA, activating the innate immune response to infections and autoimmune diseases. Upon binding to double-stranded DNA, cGAS synthesizes 2'3' cGMP-AMP, which triggers type I interferon production. Besides its presence in the cytosol and nucleus, cGAS is found at the plasma membrane, although its significance remains unclear.

Inflammatory Signaling Induces Mitochondrial Dysfunction and Neuronal Death in Traumatic Brain Injury via Downregulation of OXPHOS Genes.

Traumatic brain injury (TBI) is a major cause of neurological dysfunction and disability. This study aimed to investigate the transcriptomic changes and the functional consequences in TBI, focusing on the interplay between inflammation and mitochondrial impairment. Brain tissue samples from TBI patients and healthy controls were subjected to RNA-sequencing analysis.

Construction of a Prognostic Model for Mitochondria and Macrophage Polarization Correlation in Glioma Based on Single-Cell and Transcriptome Sequencing.

Background: Numerous diseases are associated with the interplay of mitochondrial and macrophage polarization. However, the correlation of mitochondria-related genes (MRGs) and macrophage polarization-related genes (MPRGs) with the prognosis of glioma remains unclear. This study aimed to examine this relationship based on bioinformatic analysis.

LncRNA-Snhg3 regulates mouse hepatic glycogenesis under normal chow diet.

Long noncoding RNAs (lncRNAs) are strongly associated with glucose homeostasis, but their roles remain largely unknown. In this study, the potential role of lncRNA-Snhg3 in glucose metabolism was evaluated both in vitro and in vivo. Here, we found a positive relationship between Snhg3 and hepatic glycogenesis.

Observation and Management of Juvenile Myelomonocytic Leukemia and Noonan Syndrome-Associated Myeloproliferative Disorder: A Real-World Experience.

Juvenile Myelomonocytic Leukemia (JMML) is a rare and clonal hematopoietic disorder of infancy and early childhood with myeloproliferative/myelodysplastic features resulting from germline or somatic mutations in the RAS pathway. Treatment is not uniform, with management varying from observation to stem cell transplant. The aim of our retrospective review is to describe the treatment and outcomes of a cohort of patients with JMML or Noonan Syndrome-associated Myeloproliferative Disorder (NS-MPD) to provide management guidance for this rare and heterogeneous disease.